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The map label for this gene is gatD [H]

Identifier: 226225215

GI number: 226225215

Start: 2708895

End: 2709947

Strand: Reverse

Name: gatD [H]

Synonym: Lm4b_02637

Alternate gene names: 226225215

Gene position: 2709947-2708895 (Counterclockwise)

Preceding gene: 226225216

Following gene: 226225214

Centisome position: 93.04

GC content: 41.12

Gene sequence:

>1053_bases
ATGAGAGCAGCTGTGTTATACGAGAATAATGTAATAAAAGCAGAACAAATTGATGAAGCGACTTGTGGGAAAGATCAAGT
TCGTGTCGAAGTAAAAGCAGTCGGAATATGCGGATCAGATATTCATAAAATGCAGACTCGCTGGAAATACCCACTGCCTG
CTGTAATGGGACATGAATTCGCAGGTGTAGTTACGGAAATTGGTAGCGAAGTAACAAACGTAGCTATTGGTGACCGTGTT
GCAGGGATTCCGCTCGAGCCTTGTATGGAATGTAATTATTGTAAAGCGGGCGACTTTGCATTATGTGACAACTACCGGAT
GGTGGGATCACATTTTCACGGCGGATTTGCTGAAAATGTCGTCATGAAAGCCGACAATGTCATTTCTATCGGCGACCTTG
ATTTTGAAGAAGGTGCGATGATTGAACCACTTGCTGTATCAATGCACGGAGTACTTGGTATTCAGCCGAGACTTGGCGAT
ACGGTGATTGTCTTCGGAATTGGAACCATCGGAATCTTGGTTGTACAATGCTTACTTCTTGCGGGAGTAAAAGATATTAT
CGCGGTTGATATCAGTGATAAAAAGTTAGCAGACGCGCGGGAATTCGGTTGTAAATACACCATTAATCCAAAAAATGAAG
ACTTAAAAGAACGCGTTTTTGCTTATACAAATGGTCTTGGGGCAGATATAGCGCTCGAGTGTGCTGGTTCCAAAATAACG
CAAGAACAATGTCTTCTTGTAACAAAGAAAAAAGGTAAAGTTGGTTTTCTAGGAATAGCTTACGCAGATGTGCTTTTACA
CGAGGAAGCTTTTGAAAATATTTTTAGACGTGAACTTACGCTGAAAGGTTTTTGGAATTCTTACTCGGCACCATTTCCAG
GTGAGGAGTGGCGTACGTCGATTGAATTCGTGAAACAAGGTCGAATTAAACTGAAACCACTAATTTCGCATCGTTATAAG
CTAGAAGAAACGAAAGAAGCTTTTGATATGATTCTTTCCAGAGAGCATGATTATAACAAAGTGATGATATTGCCGCAGAA
AGGTGACGATTAA

Upstream 100 bases:

>100_bases
GGGTGGTAACTTCTTTAACTGGGTTATTCTTAAATTCTCTCAAGCTTGGGCAGCTATTTTCTAAGTTTTGTCTTAATGAT
ATAGAAGAGGTGATAGTAAC

Downstream 100 bases:

>100_bases
TTTGAAAGCAGTAGTAAAAACAAATCCCGGATATGATCAAATGGAGCTAAGAGATGTAGAAGAACCACAGGTCTATGGCG
ACAAAGTAAAAATCAAAGTA

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MRAAVLYENNVIKAEQIDEATCGKDQVRVEVKAVGICGSDIHKMQTRWKYPLPAVMGHEFAGVVTEIGSEVTNVAIGDRV
AGIPLEPCMECNYCKAGDFALCDNYRMVGSHFHGGFAENVVMKADNVISIGDLDFEEGAMIEPLAVSMHGVLGIQPRLGD
TVIVFGIGTIGILVVQCLLLAGVKDIIAVDISDKKLADAREFGCKYTINPKNEDLKERVFAYTNGLGADIALECAGSKIT
QEQCLLVTKKKGKVGFLGIAYADVLLHEEAFENIFRRELTLKGFWNSYSAPFPGEEWRTSIEFVKQGRIKLKPLISHRYK
LEETKEAFDMILSREHDYNKVMILPQKGDD

Sequences:

>Translated_350_residues
MRAAVLYENNVIKAEQIDEATCGKDQVRVEVKAVGICGSDIHKMQTRWKYPLPAVMGHEFAGVVTEIGSEVTNVAIGDRV
AGIPLEPCMECNYCKAGDFALCDNYRMVGSHFHGGFAENVVMKADNVISIGDLDFEEGAMIEPLAVSMHGVLGIQPRLGD
TVIVFGIGTIGILVVQCLLLAGVKDIIAVDISDKKLADAREFGCKYTINPKNEDLKERVFAYTNGLGADIALECAGSKIT
QEQCLLVTKKKGKVGFLGIAYADVLLHEEAFENIFRRELTLKGFWNSYSAPFPGEEWRTSIEFVKQGRIKLKPLISHRYK
LEETKEAFDMILSREHDYNKVMILPQKGDD
>Mature_350_residues
MRAAVLYENNVIKAEQIDEATCGKDQVRVEVKAVGICGSDIHKMQTRWKYPLPAVMGHEFAGVVTEIGSEVTNVAIGDRV
AGIPLEPCMECNYCKAGDFALCDNYRMVGSHFHGGFAENVVMKADNVISIGDLDFEEGAMIEPLAVSMHGVLGIQPRLGD
TVIVFGIGTIGILVVQCLLLAGVKDIIAVDISDKKLADAREFGCKYTINPKNEDLKERVFAYTNGLGADIALECAGSKIT
QEQCLLVTKKKGKVGFLGIAYADVLLHEEAFENIFRRELTLKGFWNSYSAPFPGEEWRTSIEFVKQGRIKLKPLISHRYK
LEETKEAFDMILSREHDYNKVMILPQKGDD

Specific function: Converts galactitol 1-phosphate to tagatose 6-phosphate [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=328, Percent_Identity=28.3536585365854, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI71565152, Length=365, Percent_Identity=25.7534246575342, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4501929, Length=365, Percent_Identity=28.2191780821918, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI4501933, Length=365, Percent_Identity=28.7671232876712, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI34577061, Length=365, Percent_Identity=28.7671232876712, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI71565154, Length=368, Percent_Identity=26.9021739130435, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI71743840, Length=236, Percent_Identity=29.6610169491525, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI262073058, Length=236, Percent_Identity=29.6610169491525, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1788407, Length=347, Percent_Identity=34.0057636887608, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI1788073, Length=328, Percent_Identity=30.7926829268293, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1790045, Length=316, Percent_Identity=28.1645569620253, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787863, Length=346, Percent_Identity=27.4566473988439, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI226510992, Length=318, Percent_Identity=26.4150943396226, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1790718, Length=310, Percent_Identity=28.3870967741935, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1788075, Length=335, Percent_Identity=24.7761194029851, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082125, Length=347, Percent_Identity=26.8011527377522, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786552, Length=364, Percent_Identity=28.8461538461538, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786825, Length=397, Percent_Identity=24.4332493702771, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081918, Length=228, Percent_Identity=27.6315789473684, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786518, Length=134, Percent_Identity=30.5970149253731, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=350, Percent_Identity=33.7142857142857, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17562878, Length=350, Percent_Identity=32.2857142857143, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25146526, Length=371, Percent_Identity=26.4150943396226, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71997431, Length=373, Percent_Identity=26.2734584450402, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17565904, Length=318, Percent_Identity=26.4150943396226, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562584, Length=268, Percent_Identity=28.3582089552239, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71988145, Length=244, Percent_Identity=29.0983606557377, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562582, Length=332, Percent_Identity=26.2048192771084, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319955, Length=334, Percent_Identity=31.1377245508982, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6322619, Length=334, Percent_Identity=31.1377245508982, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323099, Length=327, Percent_Identity=30.2752293577982, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319257, Length=368, Percent_Identity=25.2717391304348, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320033, Length=364, Percent_Identity=26.6483516483516, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6319258, Length=384, Percent_Identity=24.21875, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319520, Length=346, Percent_Identity=27.4566473988439, Blast_Score=83, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=318, Percent_Identity=25.1572327044025, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=338, Percent_Identity=25.7396449704142, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=318, Percent_Identity=26.1006289308176, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319949, Length=130, Percent_Identity=36.1538461538462, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324486, Length=243, Percent_Identity=27.5720164609054, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17137530, Length=358, Percent_Identity=28.7709497206704, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI17737897, Length=358, Percent_Identity=29.3296089385475, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI17737895, Length=373, Percent_Identity=28.4182305630027, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.251 [H]

Molecular weight: Translated: 38728; Mature: 38728

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAAVLYENNVIKAEQIDEATCGKDQVRVEVKAVGICGSDIHKMQTRWKYPLPAVMGHEF
CCEEEEECCCEEEHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCHHHCCHH
AGVVTEIGSEVTNVAIGDRVAGIPLEPCMECNYCKAGDFALCDNYRMVGSHFHGGFAENV
HHHHHHHHHHHHEEEECCEECCCCCHHHCCCCCCCCCCEEEECCCEEECCCCCCCHHHCE
VMKADNVISIGDLDFEEGAMIEPLAVSMHGVLGIQPRLGDTVIVFGIGTIGILVVQCLLL
EEECCCEEEECCCCCCCCCEECHHHHHHCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHH
AGVKDIIAVDISDKKLADAREFGCKYTINPKNEDLKERVFAYTNGLGADIALECAGSKIT
HCCCCEEEEECCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCC
QEQCLLVTKKKGKVGFLGIAYADVLLHEEAFENIFRRELTLKGFWNSYSAPFPGEEWRTS
CCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHH
IEFVKQGRIKLKPLISHRYKLEETKEAFDMILSREHDYNKVMILPQKGDD
HHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MRAAVLYENNVIKAEQIDEATCGKDQVRVEVKAVGICGSDIHKMQTRWKYPLPAVMGHEF
CCEEEEECCCEEEHHHCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCHHHCCHH
AGVVTEIGSEVTNVAIGDRVAGIPLEPCMECNYCKAGDFALCDNYRMVGSHFHGGFAENV
HHHHHHHHHHHHEEEECCEECCCCCHHHCCCCCCCCCCEEEECCCEEECCCCCCCHHHCE
VMKADNVISIGDLDFEEGAMIEPLAVSMHGVLGIQPRLGDTVIVFGIGTIGILVVQCLLL
EEECCCEEEECCCCCCCCCEECHHHHHHCCEEECCCCCCCEEEEEEHHHHHHHHHHHHHH
AGVKDIIAVDISDKKLADAREFGCKYTINPKNEDLKERVFAYTNGLGADIALECAGSKIT
HCCCCEEEEECCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCCC
QEQCLLVTKKKGKVGFLGIAYADVLLHEEAFENIFRRELTLKGFWNSYSAPFPGEEWRTS
CCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHH
IEFVKQGRIKLKPLISHRYKLEETKEAFDMILSREHDYNKVMILPQKGDD
HHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]