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The map label for this gene is tufA

Identifier: 226225203

GI number: 226225203

Start: 2695720

End: 2696907

Strand: Reverse

Name: tufA

Synonym: Lm4b_02625

Alternate gene names: 226225203

Gene position: 2696907-2695720 (Counterclockwise)

Preceding gene: 226225204

Following gene: 226225202

Centisome position: 92.59

GC content: 41.75

Gene sequence:

>1188_bases
ATGGCAAAAGAAAAATTTGACCGCTCTAAACCCCATGTTAACATTGGTACTATTGGACACGTTGACCATGGTAAAACAAC
TTTAACTGCTGCAATTACAACTGTACTTGCTAAAAAAGGCTATGCTGATGCACAAGCTTATGACCAAATTGATGGTGCTC
CGGAAGAGAGAGAACGTGGTATCACAATCTCTACTGCTCACGTTGAGTACCAAACTGACAGCCGTCACTATGCACACGTT
GACTGCCCAGGACATGCCGATTACGTTAAAAACATGATCACTGGTGCTGCACAAATGGACGGAGCTATCTTAGTAGTATC
TGCTGCTGATGGCCCAATGCCACAAACTCGTGAACATATCTTACTTTCACGTCAAGTTGGTGTTCCATACATCGTTGTAT
TCATGAACAAATGTGACATGGTTGACGATGAAGAATTACTAGAATTAGTTGAAATGGAAATTCGTGATCTATTAACTGAA
TATGAATTCCCTGGCGATGACATTCCTGTAATCAAAGGTTCAGCTCTTAAAGCACTTCAAGGTGAAGCTGACTGGGAAGC
TAAAATTGACGAGTTAATGGAAGCTGTAGATTCTTACATTCCAACTCCAGAACGTGATACTGACAAACCATTCATGATGC
CAGTTGAGGATGTATTCTCAATCACTGGTCGTGGAACAGTTGCAACTGGACGTGTTGAACGTGGACAAGTTAAAGTTGGT
GACGAAGTAGAAGTTATCGGTATTGAAGAAGAAAGCAAAAAAGTAGTAGTAACTGGAGTAGAAATGTTCCGTAAATTACT
AGACTACGCTGAAGCTGGCGACAACATTGGCGCACTTCTACGTGGTGTTGCTCGTGAAGATATCCAACGTGGTCAAGTAT
TAGCTAAACCAGGTTCGATTACTCCACACACTAACTTCAAAGCTGAAACTTATGTTTTAACTAAAGAAGAAGGTGGACGT
CACACTCCATTCTTCAACAACTACCGCCCACAATTCTATTTCCGTACTACTGACGTAACTGGTATTGTTACACTTCCAGA
AGGTACTGAAATGGTAATGCCTGGTGATAACATTGAGCTTGCAGTTGAACTAATTGCACCAATCGCTATCGAAGACGGTA
CTAAATTCTCTATCCGTGAAGGCGGACGTACAGTAGGCGCTGGCGTTGTTTCTAACATCAGCAAATAA

Upstream 100 bases:

>100_bases
TTTCGCAACATCAAGTATAACTTTAGTTAGAAGTATTACTTAGTTTAAATTTAAGCTAAGTAAAAAATAATTATCGAATT
ATCGAGGAGGATATTTTAAA

Downstream 100 bases:

>100_bases
TATCTGATACGATTCAAACCGACAAAGTCATTTGGCTTTGTCGGTTTTTTTTGTCTAAAAAAAGCTGAATAATCTAGTAA
TAAAGAATTAGTTCATATTT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGYADAQAYDQIDGAPEERERGITISTAHVEYQTDSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKCDMVDDEELLELVEMEIRDLLTE
YEFPGDDIPVIKGSALKALQGEADWEAKIDELMEAVDSYIPTPERDTDKPFMMPVEDVFSITGRGTVATGRVERGQVKVG
DEVEVIGIEEESKKVVVTGVEMFRKLLDYAEAGDNIGALLRGVAREDIQRGQVLAKPGSITPHTNFKAETYVLTKEEGGR
HTPFFNNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNIELAVELIAPIAIEDGTKFSIREGGRTVGAGVVSNISK

Sequences:

>Translated_395_residues
MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGYADAQAYDQIDGAPEERERGITISTAHVEYQTDSRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKCDMVDDEELLELVEMEIRDLLTE
YEFPGDDIPVIKGSALKALQGEADWEAKIDELMEAVDSYIPTPERDTDKPFMMPVEDVFSITGRGTVATGRVERGQVKVG
DEVEVIGIEEESKKVVVTGVEMFRKLLDYAEAGDNIGALLRGVAREDIQRGQVLAKPGSITPHTNFKAETYVLTKEEGGR
HTPFFNNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNIELAVELIAPIAIEDGTKFSIREGGRTVGAGVVSNISK
>Mature_394_residues
AKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGYADAQAYDQIDGAPEERERGITISTAHVEYQTDSRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKCDMVDDEELLELVEMEIRDLLTEY
EFPGDDIPVIKGSALKALQGEADWEAKIDELMEAVDSYIPTPERDTDKPFMMPVEDVFSITGRGTVATGRVERGQVKVGD
EVEVIGIEEESKKVVVTGVEMFRKLLDYAEAGDNIGALLRGVAREDIQRGQVLAKPGSITPHTNFKAETYVLTKEEGGRH
TPFFNNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNIELAVELIAPIAIEDGTKFSIREGGRTVGAGVVSNISK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.3604060913706, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=30.9633027522936, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=30.9633027522936, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4503475, Length=452, Percent_Identity=27.6548672566372, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503471, Length=452, Percent_Identity=28.0973451327434, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI54607086, Length=312, Percent_Identity=28.8461538461538, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194018522, Length=348, Percent_Identity=27.2988505747126, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI46094014, Length=348, Percent_Identity=27.0114942528736, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI194018520, Length=348, Percent_Identity=27.2988505747126, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI194097354, Length=323, Percent_Identity=28.1733746130031, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4503507, Length=336, Percent_Identity=29.1666666666667, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI157426893, Length=251, Percent_Identity=27.8884462151394, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=74.8091603053435, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=74.8091603053435, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=30.3430079155673, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994988, Length=272, Percent_Identity=32.7205882352941, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789108, Length=270, Percent_Identity=34.0740740740741, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=150, Percent_Identity=32, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.6290726817043, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=43.2642487046632, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=30.1762114537445, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=30.1762114537445, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=28.4090909090909, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=427, Percent_Identity=27.6346604215457, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=427, Percent_Identity=27.6346604215457, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=354, Percent_Identity=29.0960451977401, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=27.9411764705882, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17509919, Length=273, Percent_Identity=28.9377289377289, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI32566301, Length=161, Percent_Identity=36.0248447204969, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.7056277056277, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=394, Percent_Identity=64.7208121827411, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=446, Percent_Identity=28.2511210762332, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=446, Percent_Identity=28.2511210762332, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=352, Percent_Identity=25.8522727272727, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320863, Length=297, Percent_Identity=27.9461279461279, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=347, Percent_Identity=28.5302593659942, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=30.7053941908714, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=141, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=392, Percent_Identity=57.9081632653061, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI17864358, Length=392, Percent_Identity=57.9081632653061, Blast_Score=469, Evalue=1e-132,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=47.5452196382429, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45553807, Length=459, Percent_Identity=28.7581699346405, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553816, Length=459, Percent_Identity=28.7581699346405, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24651721, Length=459, Percent_Identity=28.7581699346405, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17864154, Length=459, Percent_Identity=28.7581699346405, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24652838, Length=458, Percent_Identity=28.6026200873362, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137572, Length=458, Percent_Identity=28.6026200873362, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=31.9078947368421, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI45550900, Length=348, Percent_Identity=29.5977011494253, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137380, Length=330, Percent_Identity=28.7878787878788, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24647046, Length=356, Percent_Identity=28.0898876404494, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24647048, Length=356, Percent_Identity=28.0898876404494, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI78706572, Length=246, Percent_Identity=25.609756097561, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28572034, Length=228, Percent_Identity=32.0175438596491, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43343; Mature: 43211

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGYADAQAYDQIDGAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHCC
ITISTAHVEYQTDSRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKCDMVDDEELLELVEMEIRDLLTEYEFPGDDIPVIKGSALKALQ
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GEADWEAKIDELMEAVDSYIPTPERDTDKPFMMPVEDVFSITGRGTVATGRVERGQVKVG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCEEEEC
DEVEVIGIEEESKKVVVTGVEMFRKLLDYAEAGDNIGALLRGVAREDIQRGQVLAKPGSI
CEEEEEEECCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCC
TPHTNFKAETYVLTKEEGGRHTPFFNNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNIEL
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
AVELIAPIAIEDGTKFSIREGGRTVGAGVVSNISK
EEEEECCEEECCCCEEEECCCCCEECCHHHHCCCC
>Mature Secondary Structure 
AKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLAKKGYADAQAYDQIDGAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHCC
ITISTAHVEYQTDSRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKCDMVDDEELLELVEMEIRDLLTEYEFPGDDIPVIKGSALKALQ
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GEADWEAKIDELMEAVDSYIPTPERDTDKPFMMPVEDVFSITGRGTVATGRVERGQVKVG
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCEEEEC
DEVEVIGIEEESKKVVVTGVEMFRKLLDYAEAGDNIGALLRGVAREDIQRGQVLAKPGSI
CEEEEEEECCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCC
TPHTNFKAETYVLTKEEGGRHTPFFNNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNIEL
CCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
AVELIAPIAIEDGTKFSIREGGRTVGAGVVSNISK
EEEEECCEEECCCCEEEECCCCCEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]