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The map label for this gene is yclM [H]

Identifier: 226224924

GI number: 226224924

Start: 2420767

End: 2422128

Strand: Direct

Name: yclM [H]

Synonym: Lm4b_02344

Alternate gene names: 226224924

Gene position: 2420767-2422128 (Clockwise)

Preceding gene: 226224922

Following gene: 226224927

Centisome position: 83.11

GC content: 40.53

Gene sequence:

>1362_bases
ATGAAAGTAATTAAATTTGGCGGAAGCTCTTTAGCATCGGGGATCCAATTAAATAAGGTTTTCCAACTTGTAGCGGAGGA
TCCTGATAGGAAAATCGTTGTTGTTTCTGCACCAGGTAAACGTTTCAAGGAAGATACAAAAGTGACTGACCTACTTATTG
ATTGCGCGGCAAAGGCACTTCTCGGGGAAGATACAAGTGAATTGTTTGAATCTGTCATTGCAAGATATGCAGGGATAGCG
CTAGATACAGGGATGAGCGACGAAATTATCAATCAAATTCGCGCAGATTTACAAGCAACTATCTCATCGGACAAAAGCGA
TCCTGATAAATTTTTAGATCGAATGAAAGCTAGTGGCGAAGATAATAACGCAAAATTAATCGCCGCTTACTTTAAATTTA
AAGGCTTAAACGCCAATTATATAAATCCAAAAGATGCCGGTTTACTTGTGACAGATGAACATGCTAGCGCGCAAGTTTTA
CCAGAATCCTATGATCGTTTGTTTGCACTTCGAGAAAGAGAAGGCATTATTGTTTTCCCAGGATTTTTCGGTTATACGAA
AGACGGCGAAATTAGCACGTTTTCTAGAAGTGGCTCTGATATTACTGGAGCGATTGTGGCAAACGGCGCCCAAGCCGAGT
TATACGAGAACTTTACGGATGTGGATGCAGTCTACGCAGTCAATCCCGCTATCGTGAAAAATCCGAAGAAAGTTCTTGAA
CTAACTTACCGGGAAATGCGTGAACTTTCATATGCCGGCTTCTCTGTTTTTCATGATGAAGCTTTAATTCCGGCTTTCCA
TGCAGGTATTCCGGTGCATATTAAAAATACGAACAATCCTGATTCTTGTGGTACGCGTGTCGTTCATGAACGCGAAAATA
ACAATGGTCCAGTCGTTGGAATTGCTAGTGATGACGGCTTTTGTAGCATTTATATTAGTAAATATTTGATGAACCGAGAA
ATCGGCTTCGGACGGAAAGTGTTGCAAATTCTGGAAGATGCTGGGTTGAACTATGAACATATGCCGTCTGGGATTGATGA
TTTAACGATTATTATTCGCGAAAATCAGTTTGGCGAGGATACCGAGCAGACAATTATGTCTCGTTTGAAAGAAGAATTGA
ACGCAGATCAAGTGATTATGCAACACGGGATTTCGCTGATTATGGTTGTTGGTGAAACCATGCGCCATAATGTCGGGATT
ACGTCTCGTGCTTCAAAAGCTTTATCCGATGCGAAAGTAAATATTGAAATGATTAATCAAGGTTCTTCTGAGGTGAGCAT
AATGTTTGGTGTGAAAGAAGAACAAGAAAATACGGCCGTTCGCGCACTATACAATGAATTCTTTTCAGAAGTCTTAGTCT
AA

Upstream 100 bases:

>100_bases
CGATAATAGACAATAACTTTATCCGAAAGGTCAGAAAATTTCGCATCATCCAAAAACAACGCTTTCGGAAGCTAAAAAAT
TCAGATCAGGAGTGTGTTGC

Downstream 100 bases:

>100_bases
AAAAACAAGTTTCTCATCCTGATTGAGGAGAAACTTGTTTTTTATTATTTAATCACTTCTTCTAATTGATAGTCTTTGGC
AATTACGTCAATAATATAAT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKVIKFGGSSLASGIQLNKVFQLVAEDPDRKIVVVSAPGKRFKEDTKVTDLLIDCAAKALLGEDTSELFESVIARYAGIA
LDTGMSDEIINQIRADLQATISSDKSDPDKFLDRMKASGEDNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVL
PESYDRLFALREREGIIVFPGFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVGIASDDGFCSIYISKYLMNRE
IGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFGEDTEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGI
TSRASKALSDAKVNIEMINQGSSEVSIMFGVKEEQENTAVRALYNEFFSEVLV

Sequences:

>Translated_453_residues
MKVIKFGGSSLASGIQLNKVFQLVAEDPDRKIVVVSAPGKRFKEDTKVTDLLIDCAAKALLGEDTSELFESVIARYAGIA
LDTGMSDEIINQIRADLQATISSDKSDPDKFLDRMKASGEDNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVL
PESYDRLFALREREGIIVFPGFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVGIASDDGFCSIYISKYLMNRE
IGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFGEDTEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGI
TSRASKALSDAKVNIEMINQGSSEVSIMFGVKEEQENTAVRALYNEFFSEVLV
>Mature_453_residues
MKVIKFGGSSLASGIQLNKVFQLVAEDPDRKIVVVSAPGKRFKEDTKVTDLLIDCAAKALLGEDTSELFESVIARYAGIA
LDTGMSDEIINQIRADLQATISSDKSDPDKFLDRMKASGEDNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVL
PESYDRLFALREREGIIVFPGFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVGIASDDGFCSIYISKYLMNRE
IGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFGEDTEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGI
TSRASKALSDAKVNIEMINQGSSEVSIMFGVKEEQENTAVRALYNEFFSEVLV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=470, Percent_Identity=27.8723404255319, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1790455, Length=476, Percent_Identity=22.2689075630252, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790376, Length=317, Percent_Identity=24.9211356466877, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=375, Percent_Identity=21.8666666666667, Blast_Score=90, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49893; Mature: 49893

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKFGGSSLASGIQLNKVFQLVAEDPDRKIVVVSAPGKRFKEDTKVTDLLIDCAAKAL
CEEEEECCCHHHCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LGEDTSELFESVIARYAGIALDTGMSDEIINQIRADLQATISSDKSDPDKFLDRMKASGE
HCCCHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
DNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVLPESYDRLFALREREGIIVFP
CCCCEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEC
GFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
CCCCCCCCCCEEEECCCCCCEEEEEEECCCHHHHHHCCCCCCEEEECCCHHHCCHHHHHH
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVG
HHHHHHHHHHHCCHHHCCCCCCCHHHHCCCEEEECCCCCCCHHCCCEEEECCCCCCCEEE
IASDDGFCSIYISKYLMNREIGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFGED
EECCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCCH
TEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGITSRASKALSDAKVNIEMINQ
HHHHHHHHHHHHHCHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHCCEEEEEEECC
GSSEVSIMFGVKEEQENTAVRALYNEFFSEVLV
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVIKFGGSSLASGIQLNKVFQLVAEDPDRKIVVVSAPGKRFKEDTKVTDLLIDCAAKAL
CEEEEECCCHHHCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LGEDTSELFESVIARYAGIALDTGMSDEIINQIRADLQATISSDKSDPDKFLDRMKASGE
HCCCHHHHHHHHHHHHHCEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
DNNAKLIAAYFKFKGLNANYINPKDAGLLVTDEHASAQVLPESYDRLFALREREGIIVFP
CCCCEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEC
GFFGYTKDGEISTFSRSGSDITGAIVANGAQAELYENFTDVDAVYAVNPAIVKNPKKVLE
CCCCCCCCCCEEEECCCCCCEEEEEEECCCHHHHHHCCCCCCEEEECCCHHHCCHHHHHH
LTYREMRELSYAGFSVFHDEALIPAFHAGIPVHIKNTNNPDSCGTRVVHERENNNGPVVG
HHHHHHHHHHHCCHHHCCCCCCCHHHHCCCEEEECCCCCCCHHCCCEEEECCCCCCCEEE
IASDDGFCSIYISKYLMNREIGFGRKVLQILEDAGLNYEHMPSGIDDLTIIIRENQFGED
EECCCCEEEHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCCCCH
TEQTIMSRLKEELNADQVIMQHGISLIMVVGETMRHNVGITSRASKALSDAKVNIEMINQ
HHHHHHHHHHHHHCHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHCCEEEEEEECC
GSSEVSIMFGVKEEQENTAVRALYNEFFSEVLV
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]