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The map label for this gene is dppE [H]

Identifier: 226224804

GI number: 226224804

Start: 2290485

End: 2292161

Strand: Reverse

Name: dppE [H]

Synonym: Lm4b_02223

Alternate gene names: 226224804

Gene position: 2292161-2290485 (Counterclockwise)

Preceding gene: 226224807

Following gene: 226224803

Centisome position: 78.7

GC content: 36.14

Gene sequence:

>1677_bases
GTGAAAAAATCTAAATTATTTCTTACACTTGGATTAACACTATTACTAAGCTTAGTCTTGGTAGCATGCGGAGGCGGATC
AGATTCCAAATCCGACAAAAAAGGCTCAGATTCAGGAAAAGCTTCAGGAGAGCAAGTACTTAACTTGACAGAAAGCGCAC
TAATTCCTTCTGCAGACAGCACAAAAGCGGATGACCAAGTTGGTTTGAACGTTGTAAACCAAACAAACGAAGGTCTATAT
GCGCTTGACAAAGATGGTATTCCTGCCATTGCCGGTGCTGCTGAAGAGCCAAAAGTTAGCGATGACAAAACAGTTTATAC
AATCAAACTTCGTGAAGATGCAAAATGGTCAAACGGAGACCCTGTAACTGCAAATGACTATGTTTACTCATGGCGCCGTG
CAGTTGACCCTAACACTGCTGCAACATATTCTTACCTATTTGATGCAATCAAAAACGGTGGAGATATCGTAGCTGGCAAG
AAAAAACCTGAAGAATTAGGAATTAAAGCAGTAGATGATTATACTTTAGAAGTTACTCTATCTAAACCAACTGCTTACAT
TAACTCACTATTCGCATTCCCGACTTTCTTCCCACTTAACGAAAAATTCGTTACGGAAAAAGGCGAAAAATATGCACAAA
ATAGTGATAACATGTTATTCAATGGACCTTTCGAGTTGAAAGACTGGACTGGAACAAACAAAAAATGGACTTACGTAAAA
AATGATAAATATTGGGATAAAGATAAAGTTAAGTTGAAACAAATCAACGTACAAGTTGTTCAAGACTCCGGTACTGGACT
TAACTTATACAACACTGACAAAGTTGACCGTACTGTATTGAGTGCAGACTATGCTGCTCAAAACAAAAACAATAAAGACT
ATGTAACAGTGAATGATTCTTCTACTTTCTACATTAAATTTAACCAAAAACGTGCTGGCAAAGATACAGTATTTGCTAAC
AAAAACATCCGTAAAGCTATAGCTCTTGCGATTGACAAACAATCGTATACTGATACAGTTCTTAAAAACGGATCTAAACC
TGCAAACAACCTTGTACCAGAAGGTTTCACTTTTGACCCAGGTAACAAAGAAGATTATACAAAAGAATCTGGCAAACATT
TAGAATATGATGTAAAAGAAGCTCAAAAAGCTTGGAAAGCTGGTTTGAAAGAATTAGGAGTTAACGAAATTACAGTTGAA
TTCACTAGTGATGACACTGAAAATGCGAGAAAATCTTCTGAATTCATTCAAGACCAACTACAAAAGAACTTAGACGGTCT
TACAGTTAAACTTAAAAACGTACCATTTAAAGTTCGTTTACAAAATGACCAAAACCAAGATTACGATTTCTCTATGAGCG
GCTGGGGTCCTGACTATCAAGATCCATCCACTTTCTTAGATCTATTCGTAACTGATGGTGCACAAAACAGAATGAGTTAT
TCTAACAAAGACTACGACAAGATTTTAAATGATGCTTCTGTAACTTATGCAGCTGACGATCAAAAACGTTGGGATGAAAT
GGTTAAAGCAGAAAAAATTCTTCTTACGGATGATGTAGCTATTCAACCACTTTATCAACGTTCTACTGCATACCTACAAA
AAGACTACATTAAAAACTTGCAAAAAAATCCATTTGGTCCAGATTACACTTACAAAGAAACATACTTGACTAAATAA

Upstream 100 bases:

>100_bases
TTCATTTATCTTGTAAAAATAATTTACAAAGCCAGAATTCTTAGAGTATAATAGGTATCGAAACAATTTTTCAGAAAAAT
AAAAAAGGGAGGTCTACTTA

Downstream 100 bases:

>100_bases
AATATTAGTCTTTAAGAAATGCTTGACGGAAAGAGAGAGTATATTTCGATATGCTCTCTCCTTTTGCTGATGATTTTCAT
GTGTTATAATTAAAAATTAA

Product: pheromone ABC transporter (binding protein)

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MKKSKLFLTLGLTLLLSLVLVACGGGSDSKSDKKGSDSGKASGEQVLNLTESALIPSADSTKADDQVGLNVVNQTNEGLY
ALDKDGIPAIAGAAEEPKVSDDKTVYTIKLREDAKWSNGDPVTANDYVYSWRRAVDPNTAATYSYLFDAIKNGGDIVAGK
KKPEELGIKAVDDYTLEVTLSKPTAYINSLFAFPTFFPLNEKFVTEKGEKYAQNSDNMLFNGPFELKDWTGTNKKWTYVK
NDKYWDKDKVKLKQINVQVVQDSGTGLNLYNTDKVDRTVLSADYAAQNKNNKDYVTVNDSSTFYIKFNQKRAGKDTVFAN
KNIRKAIALAIDKQSYTDTVLKNGSKPANNLVPEGFTFDPGNKEDYTKESGKHLEYDVKEAQKAWKAGLKELGVNEITVE
FTSDDTENARKSSEFIQDQLQKNLDGLTVKLKNVPFKVRLQNDQNQDYDFSMSGWGPDYQDPSTFLDLFVTDGAQNRMSY
SNKDYDKILNDASVTYAADDQKRWDEMVKAEKILLTDDVAIQPLYQRSTAYLQKDYIKNLQKNPFGPDYTYKETYLTK

Sequences:

>Translated_558_residues
MKKSKLFLTLGLTLLLSLVLVACGGGSDSKSDKKGSDSGKASGEQVLNLTESALIPSADSTKADDQVGLNVVNQTNEGLY
ALDKDGIPAIAGAAEEPKVSDDKTVYTIKLREDAKWSNGDPVTANDYVYSWRRAVDPNTAATYSYLFDAIKNGGDIVAGK
KKPEELGIKAVDDYTLEVTLSKPTAYINSLFAFPTFFPLNEKFVTEKGEKYAQNSDNMLFNGPFELKDWTGTNKKWTYVK
NDKYWDKDKVKLKQINVQVVQDSGTGLNLYNTDKVDRTVLSADYAAQNKNNKDYVTVNDSSTFYIKFNQKRAGKDTVFAN
KNIRKAIALAIDKQSYTDTVLKNGSKPANNLVPEGFTFDPGNKEDYTKESGKHLEYDVKEAQKAWKAGLKELGVNEITVE
FTSDDTENARKSSEFIQDQLQKNLDGLTVKLKNVPFKVRLQNDQNQDYDFSMSGWGPDYQDPSTFLDLFVTDGAQNRMSY
SNKDYDKILNDASVTYAADDQKRWDEMVKAEKILLTDDVAIQPLYQRSTAYLQKDYIKNLQKNPFGPDYTYKETYLTK
>Mature_558_residues
MKKSKLFLTLGLTLLLSLVLVACGGGSDSKSDKKGSDSGKASGEQVLNLTESALIPSADSTKADDQVGLNVVNQTNEGLY
ALDKDGIPAIAGAAEEPKVSDDKTVYTIKLREDAKWSNGDPVTANDYVYSWRRAVDPNTAATYSYLFDAIKNGGDIVAGK
KKPEELGIKAVDDYTLEVTLSKPTAYINSLFAFPTFFPLNEKFVTEKGEKYAQNSDNMLFNGPFELKDWTGTNKKWTYVK
NDKYWDKDKVKLKQINVQVVQDSGTGLNLYNTDKVDRTVLSADYAAQNKNNKDYVTVNDSSTFYIKFNQKRAGKDTVFAN
KNIRKAIALAIDKQSYTDTVLKNGSKPANNLVPEGFTFDPGNKEDYTKESGKHLEYDVKEAQKAWKAGLKELGVNEITVE
FTSDDTENARKSSEFIQDQLQKNLDGLTVKLKNVPFKVRLQNDQNQDYDFSMSGWGPDYQDPSTFLDLFVTDGAQNRMSY
SNKDYDKILNDASVTYAADDQKRWDEMVKAEKILLTDDVAIQPLYQRSTAYLQKDYIKNLQKNPFGPDYTYKETYLTK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=525, Percent_Identity=31.4285714285714, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1789397, Length=563, Percent_Identity=29.3072824156306, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI87081878, Length=496, Percent_Identity=30.6451612903226, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1787762, Length=459, Percent_Identity=23.5294117647059, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787052, Length=374, Percent_Identity=25.9358288770053, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787551, Length=490, Percent_Identity=22.2448979591837, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62573; Mature: 62573

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSKLFLTLGLTLLLSLVLVACGGGSDSKSDKKGSDSGKASGEQVLNLTESALIPSADS
CCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TKADDQVGLNVVNQTNEGLYALDKDGIPAIAGAAEEPKVSDDKTVYTIKLREDAKWSNGD
CCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
PVTANDYVYSWRRAVDPNTAATYSYLFDAIKNGGDIVAGKKKPEELGIKAVDDYTLEVTL
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHCCCEEECCEEEEEEE
SKPTAYINSLFAFPTFFPLNEKFVTEKGEKYAQNSDNMLFNGPFELKDWTGTNKKWTYVK
CCCHHHHHHHHHCCCCCCCCHHHHHCCCHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEE
NDKYWDKDKVKLKQINVQVVQDSGTGLNLYNTDKVDRTVLSADYAAQNKNNKDYVTVNDS
CCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCC
STFYIKFNQKRAGKDTVFANKNIRKAIALAIDKQSYTDTVLKNGSKPANNLVPEGFTFDP
EEEEEEECCCCCCCCEEEECCCHHEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCEECC
GNKEDYTKESGKHLEYDVKEAQKAWKAGLKELGVNEITVEFTSDDTENARKSSEFIQDQL
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
QKNLDGLTVKLKNVPFKVRLQNDQNQDYDFSMSGWGPDYQDPSTFLDLFVTDGAQNRMSY
HCCCCCEEEEEECCCEEEEECCCCCCCCEEEECCCCCCCCCHHHEEEEEEECCCHHHCCC
SNKDYDKILNDASVTYAADDQKRWDEMVKAEKILLTDDVAIQPLYQRSTAYLQKDYIKNL
CCCCHHHHHCCCCEEEECCCHHHHHHHHHHCEEEEECCCHHCHHHHHHHHHHHHHHHHHH
QKNPFGPDYTYKETYLTK
HCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKSKLFLTLGLTLLLSLVLVACGGGSDSKSDKKGSDSGKASGEQVLNLTESALIPSADS
CCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
TKADDQVGLNVVNQTNEGLYALDKDGIPAIAGAAEEPKVSDDKTVYTIKLREDAKWSNGD
CCCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCC
PVTANDYVYSWRRAVDPNTAATYSYLFDAIKNGGDIVAGKKKPEELGIKAVDDYTLEVTL
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHCCCEEECCEEEEEEE
SKPTAYINSLFAFPTFFPLNEKFVTEKGEKYAQNSDNMLFNGPFELKDWTGTNKKWTYVK
CCCHHHHHHHHHCCCCCCCCHHHHHCCCHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEE
NDKYWDKDKVKLKQINVQVVQDSGTGLNLYNTDKVDRTVLSADYAAQNKNNKDYVTVNDS
CCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEECCC
STFYIKFNQKRAGKDTVFANKNIRKAIALAIDKQSYTDTVLKNGSKPANNLVPEGFTFDP
EEEEEEECCCCCCCCEEEECCCHHEEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCEECC
GNKEDYTKESGKHLEYDVKEAQKAWKAGLKELGVNEITVEFTSDDTENARKSSEFIQDQL
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHH
QKNLDGLTVKLKNVPFKVRLQNDQNQDYDFSMSGWGPDYQDPSTFLDLFVTDGAQNRMSY
HCCCCCEEEEEECCCEEEEECCCCCCCCEEEECCCCCCCCCHHHEEEEEEECCCHHHCCC
SNKDYDKILNDASVTYAADDQKRWDEMVKAEKILLTDDVAIQPLYQRSTAYLQKDYIKNL
CCCCHHHHHCCCCEEEECCCHHHHHHHHHHCEEEEECCCHHCHHHHHHHHHHHHHHHHHH
QKNPFGPDYTYKETYLTK
HCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]