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The map label for this gene is yvdE [H]

Identifier: 226224735

GI number: 226224735

Start: 2212342

End: 2213307

Strand: Reverse

Name: yvdE [H]

Synonym: Lm4b_02152

Alternate gene names: 226224735

Gene position: 2213307-2212342 (Counterclockwise)

Preceding gene: 226224736

Following gene: 226224733

Centisome position: 75.99

GC content: 37.47

Gene sequence:

>966_bases
ATGGCGACTTTAGCAGATGTTGCGAAACGGGCAAATGTCTCCAAAATGACAGTTTCACGGGTTATTAATCATCCAGATCA
AGTCTCAGATGAACTGAAAATGCTTGTTTATAGTGCAATGGAAGCCTTAGAGTATGTACCAAATTATGCTGCAAGGGCGC
TTGTTCAAAATAGGACGCAAGTTATTAAATTTTTGATTTTAGAAGAAATTGATACAGTTGAACCATATTATATGAATTTA
TTAACAGGAATTAGTCGGGAGTTAGATAAATACTACTATTCGCTTCAACTCGTTACGCAAAGATCCAGGAATATCGGAGC
TTATGACGGGCTTATTGTCACGGGAATACGCGACAAAGATTATGATTTAGGACTTTTAGATATTGATAAACCAGTTATTT
TTTATGGTGAAAATAAACGTGGCTATGATTCTATTGATGTAGATAATAAAAAAGGGACTGCGCTCGCAACAGAGCATATG
GTAGAGATCGGTTTTTCGCGAATCGTCTTTTTGGGAATTGATTTATTAGATGAACAGTTTATGAAATCGCGCCTAGAAGG
CTATGAAGAAATAGTGAAAGAACATGGTTTGGAACCGGAAAGTTATTTTATTGCGAATAGTTCCAGTGTCGCGGAAGAAA
AAGCATTTGAATTACTACGCTATAACTCAGAAAAAATTGCGATTGTTTGCGCATCAGATCGAATTGCCATTGGGGTTGTT
CGTGCTGCCGCTGCTTTTGGTCGCCGTTTTGGTGAAAATATTGCTGTAACAGGGTTTGATGGTGTTTTTTTAGATAGAAT
TTCATCGCCAAAAATTACAACTGTTCAATCTCCCGTCGTTGAAATGGGGGAAGAACTCGCGAAGATGTTACTTGCGAAAA
TTAATGATCACGGAAAACCTCAAGGAAACCTATTATTTACTCCTGAATTACTGATAAGAGCCTCTACAACTGGAAAAGAA
GAGTAA

Upstream 100 bases:

>100_bases
CAGACTTTTAATGAGAATGCTTTTTTTGTGGGTTAATTCTTATTTTTTTTTGTGTAAAAGATGGTACAATAAGGAAGATT
ATGAATGGAGGAGGTTGGAG

Downstream 100 bases:

>100_bases
TAAAAACGCTAGGAATCTTTATTCCTAGCGTTTTTTTATTTGAGTAAAATGGGTAAAAATGCGATGAAATCAAAAATGAA
GTGAACGATGAAAGTAACCC

Product: transcription regulator, LacI family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MATLADVAKRANVSKMTVSRVINHPDQVSDELKMLVYSAMEALEYVPNYAARALVQNRTQVIKFLILEEIDTVEPYYMNL
LTGISRELDKYYYSLQLVTQRSRNIGAYDGLIVTGIRDKDYDLGLLDIDKPVIFYGENKRGYDSIDVDNKKGTALATEHM
VEIGFSRIVFLGIDLLDEQFMKSRLEGYEEIVKEHGLEPESYFIANSSSVAEEKAFELLRYNSEKIAIVCASDRIAIGVV
RAAAAFGRRFGENIAVTGFDGVFLDRISSPKITTVQSPVVEMGEELAKMLLAKINDHGKPQGNLLFTPELLIRASTTGKE
E

Sequences:

>Translated_321_residues
MATLADVAKRANVSKMTVSRVINHPDQVSDELKMLVYSAMEALEYVPNYAARALVQNRTQVIKFLILEEIDTVEPYYMNL
LTGISRELDKYYYSLQLVTQRSRNIGAYDGLIVTGIRDKDYDLGLLDIDKPVIFYGENKRGYDSIDVDNKKGTALATEHM
VEIGFSRIVFLGIDLLDEQFMKSRLEGYEEIVKEHGLEPESYFIANSSSVAEEKAFELLRYNSEKIAIVCASDRIAIGVV
RAAAAFGRRFGENIAVTGFDGVFLDRISSPKITTVQSPVVEMGEELAKMLLAKINDHGKPQGNLLFTPELLIRASTTGKE
E
>Mature_320_residues
ATLADVAKRANVSKMTVSRVINHPDQVSDELKMLVYSAMEALEYVPNYAARALVQNRTQVIKFLILEEIDTVEPYYMNLL
TGISRELDKYYYSLQLVTQRSRNIGAYDGLIVTGIRDKDYDLGLLDIDKPVIFYGENKRGYDSIDVDNKKGTALATEHMV
EIGFSRIVFLGIDLLDEQFMKSRLEGYEEIVKEHGLEPESYFIANSSSVAEEKAFELLRYNSEKIAIVCASDRIAIGVVR
AAAAFGRRFGENIAVTGFDGVFLDRISSPKITTVQSPVVEMGEELAKMLLAKINDHGKPQGNLLFTPELLIRASTTGKEE

Specific function: Repressor Of The Galactose Operon. Binds Galactose As An Inducer. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=339, Percent_Identity=29.2035398230088, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790194, Length=333, Percent_Identity=29.1291291291291, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=29.7058823529412, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994940, Length=309, Percent_Identity=27.831715210356, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790369, Length=333, Percent_Identity=26.4264264264264, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788474, Length=350, Percent_Identity=26.2857142857143, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789068, Length=319, Percent_Identity=26.6457680250784, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787906, Length=346, Percent_Identity=28.3236994219653, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=26.1682242990654, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789456, Length=335, Percent_Identity=26.865671641791, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790689, Length=314, Percent_Identity=23.8853503184713, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=27.8637770897833, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35943; Mature: 35811

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLADVAKRANVSKMTVSRVINHPDQVSDELKMLVYSAMEALEYVPNYAARALVQNRTQ
CCCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VIKFLILEEIDTVEPYYMNLLTGISRELDKYYYSLQLVTQRSRNIGAYDGLIVTGIRDKD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
YDLGLLDIDKPVIFYGENKRGYDSIDVDNKKGTALATEHMVEIGFSRIVFLGIDLLDEQF
CCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKSRLEGYEEIVKEHGLEPESYFIANSSSVAEEKAFELLRYNSEKIAIVCASDRIAIGVV
HHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
RAAAAFGRRFGENIAVTGFDGVFLDRISSPKITTVQSPVVEMGEELAKMLLAKINDHGKP
HHHHHHHHHHCCCEEEECCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCC
QGNLLFTPELLIRASTTGKEE
CCCEEECHHHEEEECCCCCCC
>Mature Secondary Structure 
ATLADVAKRANVSKMTVSRVINHPDQVSDELKMLVYSAMEALEYVPNYAARALVQNRTQ
CCHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VIKFLILEEIDTVEPYYMNLLTGISRELDKYYYSLQLVTQRSRNIGAYDGLIVTGIRDKD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
YDLGLLDIDKPVIFYGENKRGYDSIDVDNKKGTALATEHMVEIGFSRIVFLGIDLLDEQF
CCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKSRLEGYEEIVKEHGLEPESYFIANSSSVAEEKAFELLRYNSEKIAIVCASDRIAIGVV
HHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHH
RAAAAFGRRFGENIAVTGFDGVFLDRISSPKITTVQSPVVEMGEELAKMLLAKINDHGKP
HHHHHHHHHHCCCEEEECCCCHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCC
QGNLLFTPELLIRASTTGKEE
CCCEEECHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]