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The map label for this gene is rnz [H]

Identifier: 226224575

GI number: 226224575

Start: 2038157

End: 2039077

Strand: Reverse

Name: rnz [H]

Synonym: Lm4b_01989

Alternate gene names: 226224575

Gene position: 2039077-2038157 (Counterclockwise)

Preceding gene: 226224579

Following gene: 226224574

Centisome position: 70.01

GC content: 38.11

Gene sequence:

>921_bases
ATGGAACTTGTTTTTCTAGGAACTGGAGCAGGCGTACCGTCGCGAGGCCGTAATGTCACATCCATCGCACTTTCAATGTT
AAATGAACGAAATACAATTTGGCTATTTGATTGCGGAGAGGCAACACAACACCAAATCATGCGAAGCCAAATTAAACTTA
GTAAACTAGAAAAAATCTTTATTACTCACATGCACGGAGATCACATTTTTGGACTGCCAGGCTTATTAAGTAGTCGTTCT
TTTCAAGGAGGGGACTCTAATTTGACTATTTACGGTCCTGCCGGAATAGCAGAATATGTCGAAACTTCCTTAAGACTCAG
TGGAACAAGGCTTACATATAAAATTAATTTTGAAGAAATAGAGCCAGGTTTGATTTTTGAAGATGAAATGTTTTTAGTTA
CAGCTGACGATCTTGATCATGGCGTACGTAGCTTTGGTTACCGTATTGTCGAAAAAGATAAACAAGGTGCACTCAATGCT
GAAAAATTAAAAGCGGACGGCGTCGAAGCGGGACCGGTTTTTCAAAAGCTGAAAAACGGCGAAATCGTAACATTAGCTGA
TGGACGCGTCATTGATGGTAAAAATTATATCGGAGAGCCGCAAAAAGGGAAGATTATTAGTATTTTTGGTGATACAAAAG
AAACTGCTAACGAACTAGAACTAGCACTGAATGCCGATATTTTGGTTCATGAAGCAACGTTTGAAGGCGATAAAGCAAAA
ATGGCAGGAGAATACATGCATTCTACAACGCTTCAAGCAGCCAATCTTGCTAAAACCGCCAATGTCAAAAAATTAATTTT
AACCCATATCAGCTCAAGATATGATCGAGATGCAAGTAAAGAACTATTAATAGAAGCAAAAACTATTTTTGAAAATACTG
AAATAGCTTATGACTTAGCTGTTTTTCCAATTGGAGAGTGA

Upstream 100 bases:

>100_bases
TTTCTGAATTATAAGGGTTTTTTCTTAGAAATGTTTATTTCCTGTTCGTTTAAGAATATGATAAAATAAGACACGAACTG
GGAAGGGAATGAAACAGATT

Downstream 100 bases:

>100_bases
CAAAATGAACCCTTTTTTGAAGAATAAAACGGTTTTAATTACCGGAGCTTCTAACGGTCTTGGAGCTGAAATCGCAAGAC
AAGTTGCTATGTCCGGTGGT

Product: hypothetical protein

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MELVFLGTGAGVPSRGRNVTSIALSMLNERNTIWLFDCGEATQHQIMRSQIKLSKLEKIFITHMHGDHIFGLPGLLSSRS
FQGGDSNLTIYGPAGIAEYVETSLRLSGTRLTYKINFEEIEPGLIFEDEMFLVTADDLDHGVRSFGYRIVEKDKQGALNA
EKLKADGVEAGPVFQKLKNGEIVTLADGRVIDGKNYIGEPQKGKIISIFGDTKETANELELALNADILVHEATFEGDKAK
MAGEYMHSTTLQAANLAKTANVKKLILTHISSRYDRDASKELLIEAKTIFENTEIAYDLAVFPIGE

Sequences:

>Translated_306_residues
MELVFLGTGAGVPSRGRNVTSIALSMLNERNTIWLFDCGEATQHQIMRSQIKLSKLEKIFITHMHGDHIFGLPGLLSSRS
FQGGDSNLTIYGPAGIAEYVETSLRLSGTRLTYKINFEEIEPGLIFEDEMFLVTADDLDHGVRSFGYRIVEKDKQGALNA
EKLKADGVEAGPVFQKLKNGEIVTLADGRVIDGKNYIGEPQKGKIISIFGDTKETANELELALNADILVHEATFEGDKAK
MAGEYMHSTTLQAANLAKTANVKKLILTHISSRYDRDASKELLIEAKTIFENTEIAYDLAVFPIGE
>Mature_306_residues
MELVFLGTGAGVPSRGRNVTSIALSMLNERNTIWLFDCGEATQHQIMRSQIKLSKLEKIFITHMHGDHIFGLPGLLSSRS
FQGGDSNLTIYGPAGIAEYVETSLRLSGTRLTYKINFEEIEPGLIFEDEMFLVTADDLDHGVRSFGYRIVEKDKQGALNA
EKLKADGVEAGPVFQKLKNGEIVTLADGRVIDGKNYIGEPQKGKIISIFGDTKETANELELALNADILVHEATFEGDKAK
MAGEYMHSTTLQAANLAKTANVKKLILTHISSRYDRDASKELLIEAKTIFENTEIAYDLAVFPIGE

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA [H]

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family [H]

Homologues:

Organism=Homo sapiens, GI8922122, Length=360, Percent_Identity=36.6666666666667, Blast_Score=224, Evalue=9e-59,
Organism=Escherichia coli, GI87082078, Length=303, Percent_Identity=52.4752475247525, Blast_Score=320, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI71985814, Length=294, Percent_Identity=29.9319727891156, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71985808, Length=294, Percent_Identity=29.9319727891156, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24652403, Length=313, Percent_Identity=28.4345047923323, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR013471 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.26.11 [H]

Molecular weight: Translated: 33714; Mature: 33714

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVFLGTGAGVPSRGRNVTSIALSMLNERNTIWLFDCGEATQHQIMRSQIKLSKLEKIF
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCEEE
ITHMHGDHIFGLPGLLSSRSFQGGDSNLTIYGPAGIAEYVETSLRLSGTRLTYKINFEEI
EEEECCCEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHEECCEEEEEEEEHHHC
EPGLIFEDEMFLVTADDLDHGVRSFGYRIVEKDKQGALNAEKLKADGVEAGPVFQKLKNG
CCCEEEECCEEEEECCCHHHHHHHHCEEEEECCCCCCCCHHHHHCCCCCCCHHHHHHCCC
EIVTLADGRVIDGKNYIGEPQKGKIISIFGDTKETANELELALNADILVHEATFEGDKAK
CEEEEECCEEECCCCCCCCCCCCCEEEEECCCCHHHHHEEEEECCEEEEEEEECCCCHHH
MAGEYMHSTTLQAANLAKTANVKKLILTHISSRYDRDASKELLIEAKTIFENTEIAYDLA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHEEEEHHHHCCCCEEEEEE
VFPIGE
EEECCC
>Mature Secondary Structure
MELVFLGTGAGVPSRGRNVTSIALSMLNERNTIWLFDCGEATQHQIMRSQIKLSKLEKIF
CEEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCEEE
ITHMHGDHIFGLPGLLSSRSFQGGDSNLTIYGPAGIAEYVETSLRLSGTRLTYKINFEEI
EEEECCCEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHEECCEEEEEEEEHHHC
EPGLIFEDEMFLVTADDLDHGVRSFGYRIVEKDKQGALNAEKLKADGVEAGPVFQKLKNG
CCCEEEECCEEEEECCCHHHHHHHHCEEEEECCCCCCCCHHHHHCCCCCCCHHHHHHCCC
EIVTLADGRVIDGKNYIGEPQKGKIISIFGDTKETANELELALNADILVHEATFEGDKAK
CEEEEECCEEECCCCCCCCCCCCCEEEEECCCCHHHHHEEEEECCEEEEEEEECCCCHHH
MAGEYMHSTTLQAANLAKTANVKKLILTHISSRYDRDASKELLIEAKTIFENTEIAYDLA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHEEEEHHHHCCCCEEEEEE
VFPIGE
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]