Click here to switch to the map view.

The map label for this gene is ubiE

Identifier: 226224535

GI number: 226224535

Start: 1993818

End: 1994531

Strand: Reverse

Name: ubiE

Synonym: Lm4b_01948

Alternate gene names: 226224535

Gene position: 1994531-1993818 (Counterclockwise)

Preceding gene: 226224536

Following gene: 226224534

Centisome position: 68.48

GC content: 39.08

Gene sequence:

>714_bases
ATGACGGAAACTAAAGAAGAAAAAGTACATAAAGTATTTGAGAAAATTTCTCCAAGTTATGACCGAATGAATAGTGTTAT
TAGTTTTAAACTACATGTGAAATGGCGTAAAGAGACAATGAAGCTAATGCGTGTTCAAAAAGGGACGAATGTTCTTGATG
TTTGCTGTGGAACGGCAGATTGGTCGATTATGATGGCGGAAGAAATTGGTCCTGAAGGTCATGTGACGGGGCTTGATTTT
AGTGAAAATATGCTGAAGGTTGGTCGCGAAAAAGTAAAAGAAGCGGATTTGCACAATGTAGAACTTATTCATGGGAATGC
AATGGAATTACCTTTTCCAGATAATAGTTTTGACTATGTGACGATTGGCTTTGGGCTTAGAAATGTACCGGATTATATGC
AAGTGCTGCGAGAAATGTACCGTGTGCTAAAACCAGGTGGACAGCTGGCATGTATTGATACGTCTCAACCCAATATTCCT
GGCTGGAAACAAGTGTTTAATGCTTATTTTCGCTATGTAATGCCTGTTTTCGGGAAGTTTTTTGCGAAAAGTTATAAAGA
ATATAGCTGGTTACAAGAATCAACGCGTGAGTTTCCAGGAATGGCTAGACTTGCGGAAATGTTTCAAGAAGCCGGATTTT
CATACGTAAGATATATTTCACACAGTGGCGGCGCGAGTGCAACCCACTTTGGATTTAAAAAGAAGGAACAATAA

Upstream 100 bases:

>100_bases
GTGAAACGGTTGAAAGATAAATCAGAACCGCTTTCTAATATACTAGGAAAAAGAATCATCGAAGTTATTAACGACTGATA
AATAGAAGGAGATTCGGGAT

Downstream 100 bases:

>100_bases
GGTGGCAAGCATGAAACTTAACTTTTTATATGCAAATATGCAAAAAGACATTGACTCAGTTGAAAAAGAACTTAAAAAGG
CCCTTTCTGGCGCAGCTGCT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTETKEEKVHKVFEKISPSYDRMNSVISFKLHVKWRKETMKLMRVQKGTNVLDVCCGTADWSIMMAEEIGPEGHVTGLDF
SENMLKVGREKVKEADLHNVELIHGNAMELPFPDNSFDYVTIGFGLRNVPDYMQVLREMYRVLKPGGQLACIDTSQPNIP
GWKQVFNAYFRYVMPVFGKFFAKSYKEYSWLQESTREFPGMARLAEMFQEAGFSYVRYISHSGGASATHFGFKKKEQ

Sequences:

>Translated_237_residues
MTETKEEKVHKVFEKISPSYDRMNSVISFKLHVKWRKETMKLMRVQKGTNVLDVCCGTADWSIMMAEEIGPEGHVTGLDF
SENMLKVGREKVKEADLHNVELIHGNAMELPFPDNSFDYVTIGFGLRNVPDYMQVLREMYRVLKPGGQLACIDTSQPNIP
GWKQVFNAYFRYVMPVFGKFFAKSYKEYSWLQESTREFPGMARLAEMFQEAGFSYVRYISHSGGASATHFGFKKKEQ
>Mature_236_residues
TETKEEKVHKVFEKISPSYDRMNSVISFKLHVKWRKETMKLMRVQKGTNVLDVCCGTADWSIMMAEEIGPEGHVTGLDFS
ENMLKVGREKVKEADLHNVELIHGNAMELPFPDNSFDYVTIGFGLRNVPDYMQVLREMYRVLKPGGQLACIDTSQPNIPG
WKQVFNAYFRYVMPVFGKFFAKSYKEYSWLQESTREFPGMARLAEMFQEAGFSYVRYISHSGGASATHFGFKKKEQ

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=265, Percent_Identity=35.0943396226415, Blast_Score=153, Evalue=1e-37,
Organism=Escherichia coli, GI48994983, Length=232, Percent_Identity=41.3793103448276, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17557039, Length=235, Percent_Identity=36.1702127659575, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17561380, Length=116, Percent_Identity=31.8965517241379, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323526, Length=249, Percent_Identity=29.3172690763052, Blast_Score=105, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24641702, Length=243, Percent_Identity=34.5679012345679, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_LISIN (P67056)

Other databases:

- EMBL:   AL596170
- PIR:   AC1688
- RefSeq:   NP_471379.1
- ProteinModelPortal:   P67056
- SMR:   P67056
- GeneID:   1130756
- GenomeReviews:   AL592022_GR
- KEGG:   lin:lin2045
- GenoList:   LIN2045
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- BioCyc:   LINN272626:LIN2045-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR014122
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR02752
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 27331; Mature: 27200

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETKEEKVHKVFEKISPSYDRMNSVISFKLHVKWRKETMKLMRVQKGTNVLDVCCGTAD
CCCCHHHHHHHHHHHHCCCHHHHHHEEEEEEEEHHHHHHHHHHHHHCCCCHHHHHCCCCC
WSIMMAEEIGPEGHVTGLDFSENMLKVGREKVKEADLHNVELIHGNAMELPFPDNSFDYV
CEEEEHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCEE
TIGFGLRNVPDYMQVLREMYRVLKPGGQLACIDTSQPNIPGWKQVFNAYFRYVMPVFGKF
EECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
FAKSYKEYSWLQESTREFPGMARLAEMFQEAGFSYVRYISHSGGASATHFGFKKKEQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHCCCCCCCC
>Mature Secondary Structure 
TETKEEKVHKVFEKISPSYDRMNSVISFKLHVKWRKETMKLMRVQKGTNVLDVCCGTAD
CCCHHHHHHHHHHHHCCCHHHHHHEEEEEEEEHHHHHHHHHHHHHCCCCHHHHHCCCCC
WSIMMAEEIGPEGHVTGLDFSENMLKVGREKVKEADLHNVELIHGNAMELPFPDNSFDYV
CEEEEHHHCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCCCCCEE
TIGFGLRNVPDYMQVLREMYRVLKPGGQLACIDTSQPNIPGWKQVFNAYFRYVMPVFGKF
EECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
FAKSYKEYSWLQESTREFPGMARLAEMFQEAGFSYVRYISHSGGASATHFGFKKKEQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669