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The map label for this gene is hepT [H]

Identifier: 226224534

GI number: 226224534

Start: 1992842

End: 1993807

Strand: Reverse

Name: hepT [H]

Synonym: Lm4b_01947

Alternate gene names: 226224534

Gene position: 1993807-1992842 (Counterclockwise)

Preceding gene: 226224535

Following gene: 226224533

Centisome position: 68.45

GC content: 38.51

Gene sequence:

>966_bases
ATGAAACTTAACTTTTTATATGCAAATATGCAAAAAGACATTGACTCAGTTGAAAAAGAACTTAAAAAGGCCCTTTCTGG
CGCAGCTGCTGAAACTACTTCTGATGCAGCACTTCATTTATTAGAGGCTGGTGGAAAACGAATTAGACCAATGTTCGTTT
GCTTATCAGCAAGACTTGCTCCTAATGCAGACTTTGATGCAGTAAAAAATGCGAGTGTAGCGATTGAATTAATTCATATG
GCGTCGATTGTGCATGATGATGTGGTGGATAATGCGGATTTAAGACGCGGACGTGAGACGATTAAATCGAAGTGGGGCAA
CCATATTGCGATGTATACCGGTGATTTTTTGTTTGCCAAATCACTGGAATATATGACAGAAATTAAAGACGTTGCTGCGC
ATAAAATGCTATCACATGTTACGGTGGAGCTTTCTACGGGTGAAATCGAACAATTAAAAGATAAATATAATTTTGACCAA
AGTGTGCGTAATTATTTACGCCGAATTAAACGGAAAACAGCTTTACTGATAGCAGCTAGTTGCGGACTCGGTGGTGTTGT
TTCTGGTCAAAGTGAAGCTGATTATCAGAAGTTATATCGTTTTGGTTATTATGTTGGCATGGCGTTTCAAATTACGGATG
ATGTACTTGATTTTGTTGGAACAGAAAAAGAACTCGGTAAGCCAGCTGGGGAAGATTTAAGACAAGGAAATGTGACATTG
CCAGTATTCTTTGCAATGGAAGATCCTTTTCTAAAAAAACGCATCAGCCAGGTTACGGAGGAAACTCCAGCAGAAGAAAT
TGCTGTTTTAGTGGAAGAAGTCAAAAAAGCGGGCGCAAAACAGGCCGAAGATGTAGCAACAGCCTACTTAAAGAAAGCTG
TAGAAATACTGGATTCTTTGCCGCAAGTGCCTGAATTAAAACCATTAAAACAAATCGTTCGTGTTTTAGATAAAAGAAAT
TATTAA

Upstream 100 bases:

>100_bases
CAAGAAGCCGGATTTTCATACGTAAGATATATTTCACACAGTGGCGGCGCGAGTGCAACCCACTTTGGATTTAAAAAGAA
GGAACAATAAGGTGGCAAGC

Downstream 100 bases:

>100_bases
CGTGAGGGAGGATTCTTTATGGAACAAACTTATGTAATGGTTAAGCCGGATGGTGTAGAACGAGGACTTATTGGTGAAAT
TGTCACAAGAATAGAGAAAA

Product: heptaprenyl diphosphate synthase component II (menaquinone biosynthesis)

Products: NA

Alternate protein names: HEPPP synthase subunit 2 [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKLNFLYANMQKDIDSVEKELKKALSGAAAETTSDAALHLLEAGGKRIRPMFVCLSARLAPNADFDAVKNASVAIELIHM
ASIVHDDVVDNADLRRGRETIKSKWGNHIAMYTGDFLFAKSLEYMTEIKDVAAHKMLSHVTVELSTGEIEQLKDKYNFDQ
SVRNYLRRIKRKTALLIAASCGLGGVVSGQSEADYQKLYRFGYYVGMAFQITDDVLDFVGTEKELGKPAGEDLRQGNVTL
PVFFAMEDPFLKKRISQVTEETPAEEIAVLVEEVKKAGAKQAEDVATAYLKKAVEILDSLPQVPELKPLKQIVRVLDKRN
Y

Sequences:

>Translated_321_residues
MKLNFLYANMQKDIDSVEKELKKALSGAAAETTSDAALHLLEAGGKRIRPMFVCLSARLAPNADFDAVKNASVAIELIHM
ASIVHDDVVDNADLRRGRETIKSKWGNHIAMYTGDFLFAKSLEYMTEIKDVAAHKMLSHVTVELSTGEIEQLKDKYNFDQ
SVRNYLRRIKRKTALLIAASCGLGGVVSGQSEADYQKLYRFGYYVGMAFQITDDVLDFVGTEKELGKPAGEDLRQGNVTL
PVFFAMEDPFLKKRISQVTEETPAEEIAVLVEEVKKAGAKQAEDVATAYLKKAVEILDSLPQVPELKPLKQIVRVLDKRN
Y
>Mature_321_residues
MKLNFLYANMQKDIDSVEKELKKALSGAAAETTSDAALHLLEAGGKRIRPMFVCLSARLAPNADFDAVKNASVAIELIHM
ASIVHDDVVDNADLRRGRETIKSKWGNHIAMYTGDFLFAKSLEYMTEIKDVAAHKMLSHVTVELSTGEIEQLKDKYNFDQ
SVRNYLRRIKRKTALLIAASCGLGGVVSGQSEADYQKLYRFGYYVGMAFQITDDVLDFVGTEKELGKPAGEDLRQGNVTL
PVFFAMEDPFLKKRISQVTEETPAEEIAVLVEEVKKAGAKQAEDVATAYLKKAVEILDSLPQVPELKPLKQIVRVLDKRN
Y

Specific function: Supplies heptaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinone menaquinone-7 (MQ-7) [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=320, Percent_Identity=29.375, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI1789578, Length=290, Percent_Identity=32.0689655172414, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1786623, Length=223, Percent_Identity=30.4932735426009, Blast_Score=78, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=240, Percent_Identity=32.0833333333333, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=275, Percent_Identity=30.9090909090909, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325188, Length=216, Percent_Identity=25.462962962963, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24651612, Length=307, Percent_Identity=28.0130293159609, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014119
- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.30 [H]

Molecular weight: Translated: 35765; Mature: 35765

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00052 RIBOSOMAL_S7 ; PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNFLYANMQKDIDSVEKELKKALSGAAAETTSDAALHLLEAGGKRIRPMFVCLSARLA
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
PNADFDAVKNASVAIELIHMASIVHDDVVDNADLRRGRETIKSKWGNHIAMYTGDFLFAK
CCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHH
SLEYMTEIKDVAAHKMLSHVTVELSTGEIEQLKDKYNFDQSVRNYLRRIKRKTALLIAAS
HHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEC
CGLGGVVSGQSEADYQKLYRFGYYVGMAFQITDDVLDFVGTEKELGKPAGEDLRQGNVTL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHCCCCEE
PVFFAMEDPFLKKRISQVTEETPAEEIAVLVEEVKKAGAKQAEDVATAYLKKAVEILDSL
EEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PQVPELKPLKQIVRVLDKRNY
CCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLNFLYANMQKDIDSVEKELKKALSGAAAETTSDAALHLLEAGGKRIRPMFVCLSARLA
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCC
PNADFDAVKNASVAIELIHMASIVHDDVVDNADLRRGRETIKSKWGNHIAMYTGDFLFAK
CCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCHHHHH
SLEYMTEIKDVAAHKMLSHVTVELSTGEIEQLKDKYNFDQSVRNYLRRIKRKTALLIAAS
HHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEC
CGLGGVVSGQSEADYQKLYRFGYYVGMAFQITDDVLDFVGTEKELGKPAGEDLRQGNVTL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHCCCCEE
PVFFAMEDPFLKKRISQVTEETPAEEIAVLVEEVKKAGAKQAEDVATAYLKKAVEILDSL
EEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PQVPELKPLKQIVRVLDKRNY
CCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7629164 [H]