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The map label for this gene is cca

Identifier: 226224509

GI number: 226224509

Start: 1966760

End: 1967941

Strand: Reverse

Name: cca

Synonym: Lm4b_01922

Alternate gene names: 226224509

Gene position: 1967941-1966760 (Counterclockwise)

Preceding gene: 226224510

Following gene: 226224508

Centisome position: 67.56

GC content: 38.92

Gene sequence:

>1182_bases
ATGAATGACGTTTTCCTAAAAGCGCTTCCTGTATTGCAAAAATTAACTACAGCGGGTTTTGAAGCGTATTTTGTTGGCGG
ATCTGTCAGAGATTACTTGCTAAATAGAACGATTTCCGATGTGGATATTGCAACTAGTGCTTTCCCAGAAGAAGTAAAAG
AGATTTTTCAGACAAGTTATGACACTGGGATTGCACATGGGACTGTTACAGTTAGAGAAAATAATGAATTTTATGAAGTG
ACCACTTTTCGAACGGAAGGGACGTATGAAGATTTCAGGCGCCCAAGCGAGGTAACCTTTATTCGCTCCCTAGAAGAGGA
TTTAAAACGTCGCGATTTTACGATGAACGCCATTGCGATGGATGAACATTTTGCGCTACAAGACCCGTTTTCTGGGCAAC
TAGCGATTCAAAATAAAGAAATCAAAGCAGTTGGTAAAGCCTCCGAACGTTTTCATGAAGATGCGCTTAGAATGATGCGA
GCAGTCCGCTTTCTGAGTCAACTTGACTTTGAGCTTGATAAAGAAACCGAAAAAGCTTTAGAAAGCCAAATAGAGTTGTT
ACAGCATACTTCCGTTGAACGAATTACGGTTGAATGGCTAAAAATGATGAAAGGCAAAGCAGCAAAACGTGCGATAGAAC
TACTTTTAAAAGTCAAAATGGAAACTTATTTACCAGGCTTAAAAGACGAGAAATCAGCACTAAGCGAGTTTGCTAGTTGG
GACTGGGAAAAACGAACAACCGAAGAATCGATTTGGCTTGGACTCGTCGTTGCTGTCAAACCTAACAATGTTAACGCATT
TCTTAAAGCTTGGAAATTACCCAATAAAACAATTCAACTTGTGAATAAAGCATATCAATACGCGCTAAACATGAAAGAGA
CATGGCTAACAGAAGAACTTTATCATGCTGGCAAGGCAGTTTTCTCATTAGTAAATGAACTAAATGTTATTCGAGGGCAA
GAAAACAACCAACATAAAGTAAGTCAAGCATACGAGGCGTTACCAATTCACTCCAAAAAAGACTTAGCTATTACTGGAGC
TGATTTATTAAAATGGTCTGGAGAAAGCGCAGGTCCATGGGTAAAAGAGACGCTTGATAAAGTGGAATGTGGTGTCCTTT
CCAATGAAATAAACAATGAAAAAATTCAGATTAAAAGGTGGCTAGGCTATCATGAAGAATAA

Upstream 100 bases:

>100_bases
CAAATATTGGCACAGCAGAAATTTTAATTCGTGGTTTAGGAGCTGGTTTCCTAGATTGGCGAGACTTAAGACGGAATGAT
GAATAAAGGAGCGATGACAG

Downstream 100 bases:

>100_bases
TCGAGAAAAATTACTTGCTTTATTTACAGAAAGTGATGGTACTTATTTATCAGGGCAAGAAATTGCGGATAGTCTAGGAT
GCTCGCGCACAGCCGTTTGG

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNDVFLKALPVLQKLTTAGFEAYFVGGSVRDYLLNRTISDVDIATSAFPEEVKEIFQTSYDTGIAHGTVTVRENNEFYEV
TTFRTEGTYEDFRRPSEVTFIRSLEEDLKRRDFTMNAIAMDEHFALQDPFSGQLAIQNKEIKAVGKASERFHEDALRMMR
AVRFLSQLDFELDKETEKALESQIELLQHTSVERITVEWLKMMKGKAAKRAIELLLKVKMETYLPGLKDEKSALSEFASW
DWEKRTTEESIWLGLVVAVKPNNVNAFLKAWKLPNKTIQLVNKAYQYALNMKETWLTEELYHAGKAVFSLVNELNVIRGQ
ENNQHKVSQAYEALPIHSKKDLAITGADLLKWSGESAGPWVKETLDKVECGVLSNEINNEKIQIKRWLGYHEE

Sequences:

>Translated_393_residues
MNDVFLKALPVLQKLTTAGFEAYFVGGSVRDYLLNRTISDVDIATSAFPEEVKEIFQTSYDTGIAHGTVTVRENNEFYEV
TTFRTEGTYEDFRRPSEVTFIRSLEEDLKRRDFTMNAIAMDEHFALQDPFSGQLAIQNKEIKAVGKASERFHEDALRMMR
AVRFLSQLDFELDKETEKALESQIELLQHTSVERITVEWLKMMKGKAAKRAIELLLKVKMETYLPGLKDEKSALSEFASW
DWEKRTTEESIWLGLVVAVKPNNVNAFLKAWKLPNKTIQLVNKAYQYALNMKETWLTEELYHAGKAVFSLVNELNVIRGQ
ENNQHKVSQAYEALPIHSKKDLAITGADLLKWSGESAGPWVKETLDKVECGVLSNEINNEKIQIKRWLGYHEE
>Mature_393_residues
MNDVFLKALPVLQKLTTAGFEAYFVGGSVRDYLLNRTISDVDIATSAFPEEVKEIFQTSYDTGIAHGTVTVRENNEFYEV
TTFRTEGTYEDFRRPSEVTFIRSLEEDLKRRDFTMNAIAMDEHFALQDPFSGQLAIQNKEIKAVGKASERFHEDALRMMR
AVRFLSQLDFELDKETEKALESQIELLQHTSVERITVEWLKMMKGKAAKRAIELLLKVKMETYLPGLKDEKSALSEFASW
DWEKRTTEESIWLGLVVAVKPNNVNAFLKAWKLPNKTIQLVNKAYQYALNMKETWLTEELYHAGKAVFSLVNELNVIRGQ
ENNQHKVSQAYEALPIHSKKDLAITGADLLKWSGESAGPWVKETLDKVECGVLSNEINNEKIQIKRWLGYHEE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=248, Percent_Identity=33.0645161290323, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI87081691, Length=229, Percent_Identity=32.3144104803493, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789436, Length=184, Percent_Identity=32.0652173913043, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=370, Percent_Identity=27.5675675675676, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281360112, Length=195, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21357337, Length=195, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_LISMC (C1KWK5)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002758616.1
- GeneID:   7703217
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_01922
- OMA:   GKASERF
- ProtClustDB:   PRK13299
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45144; Mature: 45144

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: BINDING 27-27 BINDING 30-30 BINDING 111-111 BINDING 154-154 BINDING 157-157 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDVFLKALPVLQKLTTAGFEAYFVGGSVRDYLLNRTISDVDIATSAFPEEVKEIFQTSY
CCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCC
DTGIAHGTVTVRENNEFYEVTTFRTEGTYEDFRRPSEVTFIRSLEEDLKRRDFTMNAIAM
CCCCCCCEEEEEECCCEEEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEE
DEHFALQDPFSGQLAIQNKEIKAVGKASERFHEDALRMMRAVRFLSQLDFELDKETEKAL
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ESQIELLQHTSVERITVEWLKMMKGKAAKRAIELLLKVKMETYLPGLKDEKSALSEFASW
HHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
DWEKRTTEESIWLGLVVAVKPNNVNAFLKAWKLPNKTIQLVNKAYQYALNMKETWLTEEL
CCHHCCCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
YHAGKAVFSLVNELNVIRGQENNQHKVSQAYEALPIHSKKDLAITGADLLKWSGESAGPW
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHCCCCCCCCH
VKETLDKVECGVLSNEINNEKIQIKRWLGYHEE
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MNDVFLKALPVLQKLTTAGFEAYFVGGSVRDYLLNRTISDVDIATSAFPEEVKEIFQTSY
CCCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHCC
DTGIAHGTVTVRENNEFYEVTTFRTEGTYEDFRRPSEVTFIRSLEEDLKRRDFTMNAIAM
CCCCCCCEEEEEECCCEEEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEE
DEHFALQDPFSGQLAIQNKEIKAVGKASERFHEDALRMMRAVRFLSQLDFELDKETEKAL
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
ESQIELLQHTSVERITVEWLKMMKGKAAKRAIELLLKVKMETYLPGLKDEKSALSEFASW
HHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
DWEKRTTEESIWLGLVVAVKPNNVNAFLKAWKLPNKTIQLVNKAYQYALNMKETWLTEEL
CCHHCCCCCCEEEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
YHAGKAVFSLVNELNVIRGQENNQHKVSQAYEALPIHSKKDLAITGADLLKWSGESAGPW
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHCCCCCCCCH
VKETLDKVECGVLSNEINNEKIQIKRWLGYHEE
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA