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The map label for this gene is birA

Identifier: 226224508

GI number: 226224508

Start: 1965793

End: 1966770

Strand: Reverse

Name: birA

Synonym: Lm4b_01921

Alternate gene names: 226224508

Gene position: 1966770-1965793 (Counterclockwise)

Preceding gene: 226224509

Following gene: 226224507

Centisome position: 67.52

GC content: 39.26

Gene sequence:

>978_bases
ATGAAGAATAATCGAGAAAAATTACTTGCTTTATTTACAGAAAGTGATGGTACTTATTTATCAGGGCAAGAAATTGCGGA
TAGTCTAGGATGCTCGCGCACAGCCGTTTGGAAGCAAATGGAAGCTCTCCGTAAAGAAGGATTTGAAATCGAAGCAGTCA
GAAACCGTGGTTACCGTTTGTCAGCAACAGCAGAACAGTACACGAAAGATGCACTACTACTCGGTTTAGAAACCAAATTT
ATCGGTCAGCATTTGGAAATCCATGAATCGGTTAGCTCTACACAAATCATCGCCCATCAGCAAATCGAATCTAGCCCTGA
AGGAACCGTTATCGTAGCCGATGAGCAAACAGCTGGAAAAGGTCGCTTGCTTCGCCCTTGGAGTTCCAAAAAAGGGGAAG
GTATTTGGATGAGTGTAATTTTAAAACCACAAATCCCTATTCAAAAAGTACCCCAGTTCACGTTTATCGCCTCACTCGCA
ATCACAGAAGCGATTGAAAATAGCACAAAGCTCGAACCGAAAATCAAATGGCCGAATGATATTTACATTGGTAAACGAAA
AATCTGTGGTGTTTTAACGGAAATGCAAGCGGAGGCAGAAACGATTCACGCAGTAATTATCGGTATGGGAATCAATGTAA
ATCAACAGGAATTCCCAGAAGAAATTAAAGACAAAGCAAGTTCTCTAAAACTGGAATTAGGCGAAAGCGTCTCAAGAAAA
GCTTTACTACAAGAAATTCTCACTTCTTTAGAAAAATATTATGAGCTTTTTCTAGATAAAGGATTCGCACCGATAAAATT
ACTTTGGGAAACAAAAGCCATTCCATTTGGCGAAAAATTAACTGCTAGCACAACAAAAGGCAAAATTCATGGACAAGTAA
AAGGGATTTCTGATGAAGGTGTACTCTTACTCCAAGACGCACTCGGTGAAGTACATTCAATCTATTCCGCAGATATTTTG
CTAGATAATGAAAAATAA

Upstream 100 bases:

>100_bases
GGTCCATGGGTAAAAGAGACGCTTGATAAAGTGGAATGTGGTGTCCTTTCCAATGAAATAAACAATGAAAAAATTCAGAT
TAAAAGGTGGCTAGGCTATC

Downstream 100 bases:

>100_bases
CGAAACACTATAGAAAAGAACGTTTTAACCCTAATAAAATACCCGAAAACATATCTTTTAACTCGAAAACATAGTGTGAA
AATCACTTTTTCGGGTTTTT

Product: transcriptional regulator and biotin acetyl-CoA-carboxylase synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKNNREKLLALFTESDGTYLSGQEIADSLGCSRTAVWKQMEALRKEGFEIEAVRNRGYRLSATAEQYTKDALLLGLETKF
IGQHLEIHESVSSTQIIAHQQIESSPEGTVIVADEQTAGKGRLLRPWSSKKGEGIWMSVILKPQIPIQKVPQFTFIASLA
ITEAIENSTKLEPKIKWPNDIYIGKRKICGVLTEMQAEAETIHAVIIGMGINVNQQEFPEEIKDKASSLKLELGESVSRK
ALLQEILTSLEKYYELFLDKGFAPIKLLWETKAIPFGEKLTASTTKGKIHGQVKGISDEGVLLLQDALGEVHSIYSADIL
LDNEK

Sequences:

>Translated_325_residues
MKNNREKLLALFTESDGTYLSGQEIADSLGCSRTAVWKQMEALRKEGFEIEAVRNRGYRLSATAEQYTKDALLLGLETKF
IGQHLEIHESVSSTQIIAHQQIESSPEGTVIVADEQTAGKGRLLRPWSSKKGEGIWMSVILKPQIPIQKVPQFTFIASLA
ITEAIENSTKLEPKIKWPNDIYIGKRKICGVLTEMQAEAETIHAVIIGMGINVNQQEFPEEIKDKASSLKLELGESVSRK
ALLQEILTSLEKYYELFLDKGFAPIKLLWETKAIPFGEKLTASTTKGKIHGQVKGISDEGVLLLQDALGEVHSIYSADIL
LDNEK
>Mature_325_residues
MKNNREKLLALFTESDGTYLSGQEIADSLGCSRTAVWKQMEALRKEGFEIEAVRNRGYRLSATAEQYTKDALLLGLETKF
IGQHLEIHESVSSTQIIAHQQIESSPEGTVIVADEQTAGKGRLLRPWSSKKGEGIWMSVILKPQIPIQKVPQFTFIASLA
ITEAIENSTKLEPKIKWPNDIYIGKRKICGVLTEMQAEAETIHAVIIGMGINVNQQEFPEEIKDKASSLKLELGESVSRK
ALLQEILTSLEKYYELFLDKGFAPIKLLWETKAIPFGEKLTASTTKGKIHGQVKGISDEGVLLLQDALGEVHSIYSADIL
LDNEK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=311, Percent_Identity=27.0096463022508, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1790408, Length=309, Percent_Identity=25.5663430420712, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36154; Mature: 36154

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNREKLLALFTESDGTYLSGQEIADSLGCSRTAVWKQMEALRKEGFEIEAVRNRGYRL
CCCCCEEEEEEEECCCCCEECHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCEEE
SATAEQYTKDALLLGLETKFIGQHLEIHESVSSTQIIAHQQIESSPEGTVIVADEQTAGK
ECCHHHHCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
GRLLRPWSSKKGEGIWMSVILKPQIPIQKVPQFTFIASLAITEAIENSTKLEPKIKWPND
CCEECCCCCCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCCCC
IYIGKRKICGVLTEMQAEAETIHAVIIGMGINVNQQEFPEEIKDKASSLKLELGESVSRK
EEECHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHEEEHHCCHHHHH
ALLQEILTSLEKYYELFLDKGFAPIKLLWETKAIPFGEKLTASTTKGKIHGQVKGISDEG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCC
VLLLQDALGEVHSIYSADILLDNEK
EEEEHHHHHHHHHHHEEEEEEECCC
>Mature Secondary Structure
MKNNREKLLALFTESDGTYLSGQEIADSLGCSRTAVWKQMEALRKEGFEIEAVRNRGYRL
CCCCCEEEEEEEECCCCCEECHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCEEE
SATAEQYTKDALLLGLETKFIGQHLEIHESVSSTQIIAHQQIESSPEGTVIVADEQTAGK
ECCHHHHCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCC
GRLLRPWSSKKGEGIWMSVILKPQIPIQKVPQFTFIASLAITEAIENSTKLEPKIKWPND
CCEECCCCCCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCCCCCCCEECCCCC
IYIGKRKICGVLTEMQAEAETIHAVIIGMGINVNQQEFPEEIKDKASSLKLELGESVSRK
EEECHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHEEEHHCCHHHHH
ALLQEILTSLEKYYELFLDKGFAPIKLLWETKAIPFGEKLTASTTKGKIHGQVKGISDEG
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCC
VLLLQDALGEVHSIYSADILLDNEK
EEEEHHHHHHHHHHHEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]