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The map label for this gene is asnC

Identifier: 226224500

GI number: 226224500

Start: 1956902

End: 1958194

Strand: Reverse

Name: asnC

Synonym: Lm4b_01913

Alternate gene names: 226224500

Gene position: 1958194-1956902 (Counterclockwise)

Preceding gene: 226224501

Following gene: 226224499

Centisome position: 67.23

GC content: 39.91

Gene sequence:

>1293_bases
GTGAAAATTACAATCAATCAAGCATCCGAATTTGTCGGAAAAGAAGTAACTATTGGAGCATGGCTTGCGAATAAGCGTTC
AAGTGGTAAAATTGCTTTCTTACAATTACGTGATGGAACAGGATTTATGCAAGGCGTTGTCGTAAAAGCAGAAGTTGGCG
ATGATATTTTCGCAACTGCTAAAGCATTAACCCAAGAAACAAGTTTATATGTCACAGGTACAATTAACGAAGATACTCGT
TCACCATTTGGTTATGAAATGGCTGTATCTGGTGTAGAAGTAATTAGCGAATCACATGATTATCCAATTACACCAAAAGA
GCATGGCACGGAATTTTTAATGGACCACCGTCATTTATGGCTACGTTCTAACCGTCAACATGCGATTATGAAAATTCGTA
ACGAAATTATCCGCGCTAGCTATGAGTTTTTCAATAAAGAAGGATTCCTAAAAATCGATCCACCAATCTTGACCGGAAGT
GCTCCAGAAGGAACAACCGAACTTTTCCATACGAAATATTTTGATGAAGATGCGTTTCTATCTCAAAGTGGTCAATTATA
CATGGAAGCAGCAGCAATGGCTTTCGGTAAAGTATTCTCATTCGGTCCAACTTTCCGTGCTGAGAAATCAAAAACTCGCC
GTCACTTAATTGAATTCTGGATGATTGAACCGGAAATGGCATTTTACAAATTAGAAGATAGCTTACAAGTACAAGAAAAC
TACGTGGCATTCCTAGTAAAAGCAGTTCTTGATAACTGCCGCTTAGAACTAGATCGCCTTGGTCGTGATGTTAGTCATTT
AGAAAAAATGGTTGCACCGTTTCCGCGTATTACTTATACAGAAGCAATCGAACGTTTACACGAATTAGGCTTTGATGATA
TTGTTTGGGGAGATGACTTCGGGGCACCACATGAAACAGCGATTGCAGACAGCTTCGAAAAACCAGTTTTCATTACACAT
TATCCAAAAGCAATTAAACCATTTTATATGCCGGAAGATCCAGAAAACGATCAAGTTGTGTTGTGCGCTGATATGATTGC
TCCAGAAGGATACGGAGAAATCATTGGTGGATCGGAACGTATTCACGATTTAGAAACGCTTCAAGCTAGAATGGAAGACT
TCGATTTGGATCAAGAAGCCTACAGCTGGTACCTTGACTTAGCTCGTTATGGCTCTGTTCCACATTCCGGTTTTGGTCTT
GGCTTAGAACGTACAGTTGCTTGGATTTCGGGCACAGAACATGTACGTGAAACCATTCCGTTCCCACGTTTACTAAACCG
TTTATATCCGTAA

Upstream 100 bases:

>100_bases
ATGCCTGATTATATACGCCTTTCTTATGCAACAAATCCAGACTTATTCCAAGAAGCTATAAATCGAATCAAAAGTTTTAT
GAAATAGGGAGTGTAGACAA

Downstream 100 bases:

>100_bases
GTTAAAAAGTGAAGCCAACATACTGGCTTCACTTTTTTTGTGGACTTTAAATTGAAAATAATCTTTTCCGCGGGATTTCA
TGATTTTAGCAGTACTTTTA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKITINQASEFVGKEVTIGAWLANKRSSGKIAFLQLRDGTGFMQGVVVKAEVGDDIFATAKALTQETSLYVTGTINEDTR
SPFGYEMAVSGVEVISESHDYPITPKEHGTEFLMDHRHLWLRSNRQHAIMKIRNEIIRASYEFFNKEGFLKIDPPILTGS
APEGTTELFHTKYFDEDAFLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFYKLEDSLQVQEN
YVAFLVKAVLDNCRLELDRLGRDVSHLEKMVAPFPRITYTEAIERLHELGFDDIVWGDDFGAPHETAIADSFEKPVFITH
YPKAIKPFYMPEDPENDQVVLCADMIAPEGYGEIIGGSERIHDLETLQARMEDFDLDQEAYSWYLDLARYGSVPHSGFGL
GLERTVAWISGTEHVRETIPFPRLLNRLYP

Sequences:

>Translated_430_residues
MKITINQASEFVGKEVTIGAWLANKRSSGKIAFLQLRDGTGFMQGVVVKAEVGDDIFATAKALTQETSLYVTGTINEDTR
SPFGYEMAVSGVEVISESHDYPITPKEHGTEFLMDHRHLWLRSNRQHAIMKIRNEIIRASYEFFNKEGFLKIDPPILTGS
APEGTTELFHTKYFDEDAFLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFYKLEDSLQVQEN
YVAFLVKAVLDNCRLELDRLGRDVSHLEKMVAPFPRITYTEAIERLHELGFDDIVWGDDFGAPHETAIADSFEKPVFITH
YPKAIKPFYMPEDPENDQVVLCADMIAPEGYGEIIGGSERIHDLETLQARMEDFDLDQEAYSWYLDLARYGSVPHSGFGL
GLERTVAWISGTEHVRETIPFPRLLNRLYP
>Mature_430_residues
MKITINQASEFVGKEVTIGAWLANKRSSGKIAFLQLRDGTGFMQGVVVKAEVGDDIFATAKALTQETSLYVTGTINEDTR
SPFGYEMAVSGVEVISESHDYPITPKEHGTEFLMDHRHLWLRSNRQHAIMKIRNEIIRASYEFFNKEGFLKIDPPILTGS
APEGTTELFHTKYFDEDAFLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFYKLEDSLQVQEN
YVAFLVKAVLDNCRLELDRLGRDVSHLEKMVAPFPRITYTEAIERLHELGFDDIVWGDDFGAPHETAIADSFEKPVFITH
YPKAIKPFYMPEDPENDQVVLCADMIAPEGYGEIIGGSERIHDLETLQARMEDFDLDQEAYSWYLDLARYGSVPHSGFGL
GLERTVAWISGTEHVRETIPFPRLLNRLYP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=445, Percent_Identity=35.2808988764045, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI4758762, Length=447, Percent_Identity=32.4384787472036, Blast_Score=235, Evalue=7e-62,
Organism=Homo sapiens, GI45439306, Length=463, Percent_Identity=29.8056155507559, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787161, Length=455, Percent_Identity=38.021978021978, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1790571, Length=462, Percent_Identity=23.5930735930736, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789256, Length=465, Percent_Identity=23.2258064516129, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788173, Length=245, Percent_Identity=27.7551020408163, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=448, Percent_Identity=34.5982142857143, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17556372, Length=411, Percent_Identity=29.683698296837, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17551876, Length=453, Percent_Identity=27.5938189845475, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI71994340, Length=474, Percent_Identity=23.8396624472574, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535925, Length=474, Percent_Identity=23.8396624472574, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17535927, Length=474, Percent_Identity=23.8396624472574, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321807, Length=444, Percent_Identity=33.7837837837838, Blast_Score=250, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6319872, Length=452, Percent_Identity=30.9734513274336, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=455, Percent_Identity=28.7912087912088, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI19921528, Length=444, Percent_Identity=34.009009009009, Blast_Score=231, Evalue=1e-60,
Organism=Drosophila melanogaster, GI19922416, Length=443, Percent_Identity=30.0225733634311, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI17136276, Length=464, Percent_Identity=28.2327586206897, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 48955; Mature: 48955

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITINQASEFVGKEVTIGAWLANKRSSGKIAFLQLRDGTGFMQGVVVKAEVGDDIFATA
CEEEECCHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCCHHHHHH
KALTQETSLYVTGTINEDTRSPFGYEMAVSGVEVISESHDYPITPKEHGTEFLMDHRHLW
HHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHE
LRSNRQHAIMKIRNEIIRASYEFFNKEGFLKIDPPILTGSAPEGTTELFHTKYFDEDAFL
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCHHHHHHHHCCCHHHH
SQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFYKLEDSLQVQEN
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHH
YVAFLVKAVLDNCRLELDRLGRDVSHLEKMVAPFPRITYTEAIERLHELGFDDIVWGDDF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCC
GAPHETAIADSFEKPVFITHYPKAIKPFYMPEDPENDQVVLCADMIAPEGYGEIIGGSER
CCCCHHHHHHCCCCCEEEEECCHHCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCHH
IHDLETLQARMEDFDLDQEAYSWYLDLARYGSVPHSGFGLGLERTVAWISGTEHVRETIP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHCC
FPRLLNRLYP
HHHHHHHHCC
>Mature Secondary Structure
MKITINQASEFVGKEVTIGAWLANKRSSGKIAFLQLRDGTGFMQGVVVKAEVGDDIFATA
CEEEECCHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEEECCCHHHHHH
KALTQETSLYVTGTINEDTRSPFGYEMAVSGVEVISESHDYPITPKEHGTEFLMDHRHLW
HHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHE
LRSNRQHAIMKIRNEIIRASYEFFNKEGFLKIDPPILTGSAPEGTTELFHTKYFDEDAFL
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCCHHHHHHHHCCCHHHH
SQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFYKLEDSLQVQEN
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHH
YVAFLVKAVLDNCRLELDRLGRDVSHLEKMVAPFPRITYTEAIERLHELGFDDIVWGDDF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCC
GAPHETAIADSFEKPVFITHYPKAIKPFYMPEDPENDQVVLCADMIAPEGYGEIIGGSER
CCCCHHHHHHCCCCCEEEEECCHHCCCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCHH
IHDLETLQARMEDFDLDQEAYSWYLDLARYGSVPHSGFGLGLERTVAWISGTEHVRETIP
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHCC
FPRLLNRLYP
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]