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The map label for this gene is nth

Identifier: 226224498

GI number: 226224498

Start: 1955363

End: 1956022

Strand: Reverse

Name: nth

Synonym: Lm4b_01911

Alternate gene names: 226224498

Gene position: 1956022-1955363 (Counterclockwise)

Preceding gene: 226224499

Following gene: 226224497

Centisome position: 67.16

GC content: 38.48

Gene sequence:

>660_bases
TTGTTATCGAATAAACAAACCGTATTATGCATAGAAGAAATGGCAAAAATGTTTCCAGCAGCACACTGTGAGCTAGTTCA
TAAAAATACGTTTGAATTACTGGTGGCTGTTGTCTTATCCGCTCAATGTACAGATGTGTTAGTAAATCGCGTGACTGCCT
CGCTGTTTGAAAAATATCACTCACCAGAAGATTATTTGGCTGTTCCACTGGAAGAATTGATGGAAGATATTCGCTCTATT
GGCTTATATCGTAATAAAGCAAAAAATATTCAAGGATTATCCGAGAAAATTCTAATCGAATTTAATGGGGAAGTACCAAA
GACGCATGCGGAACTTGAGAGCCTTCCAGGTGTAGGTAGAAAAACGGCCAATGTTGTTTTATCAGTTGGCTTTGGTATTC
CAGCAATCGCAGTAGATACACATGTCGAACGAATTAGTAAACGCTTAGGGATTTGTAGATGGAAAGATTCTGTTGTAGAA
GTGGAAGAAACGTTAAAAAGAAAGCTACCAAAAGAACTCTGGTCTGATGCACATCATTATATGATTTTCTTTGGAAGATA
TCATTGTAAAGCAAGAAACCCAGAATGCCCGACCTGTCCCTTACTCTATTTATGCAGAGAAGGAAAAAAACAAGCGAAAG
TGAGGGGATTCGATGGCTGA

Upstream 100 bases:

>100_bases
CGCACAAGCCAAGCAATTAACCAAACGGAAGTGAAAAAAAGCGCAGGTTCGATTCCTCTATACGACTGGCTTGAAAAAAG
AAAAGGGTGAGGTACACAAA

Downstream 100 bases:

>100_bases
ATTTAAATATGCAGTAGAATTAACACATCCTGATTTCCCAGCACCGGATGTTATTTTAGAAGAATATGATCCAGAATCCA
TTTATGTTAGTGGTCTACCT

Product: endonuclease III (DNA repair)

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLSNKQTVLCIEEMAKMFPAAHCELVHKNTFELLVAVVLSAQCTDVLVNRVTASLFEKYHSPEDYLAVPLEELMEDIRSI
GLYRNKAKNIQGLSEKILIEFNGEVPKTHAELESLPGVGRKTANVVLSVGFGIPAIAVDTHVERISKRLGICRWKDSVVE
VEETLKRKLPKELWSDAHHYMIFFGRYHCKARNPECPTCPLLYLCREGKKQAKVRGFDG

Sequences:

>Translated_219_residues
MLSNKQTVLCIEEMAKMFPAAHCELVHKNTFELLVAVVLSAQCTDVLVNRVTASLFEKYHSPEDYLAVPLEELMEDIRSI
GLYRNKAKNIQGLSEKILIEFNGEVPKTHAELESLPGVGRKTANVVLSVGFGIPAIAVDTHVERISKRLGICRWKDSVVE
VEETLKRKLPKELWSDAHHYMIFFGRYHCKARNPECPTCPLLYLCREGKKQAKVRGFDG
>Mature_219_residues
MLSNKQTVLCIEEMAKMFPAAHCELVHKNTFELLVAVVLSAQCTDVLVNRVTASLFEKYHSPEDYLAVPLEELMEDIRSI
GLYRNKAKNIQGLSEKILIEFNGEVPKTHAELESLPGVGRKTANVVLSVGFGIPAIAVDTHVERISKRLGICRWKDSVVE
VEETLKRKLPKELWSDAHHYMIFFGRYHCKARNPECPTCPLLYLCREGKKQAKVRGFDG

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=180, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI115298650, Length=194, Percent_Identity=25.2577319587629, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI115298654, Length=194, Percent_Identity=25.2577319587629, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI115298652, Length=194, Percent_Identity=25.2577319587629, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI190358497, Length=194, Percent_Identity=25.2577319587629, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI6912520, Length=194, Percent_Identity=25.2577319587629, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI115298648, Length=194, Percent_Identity=25.2577319587629, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787920, Length=192, Percent_Identity=46.875, Blast_Score=164, Evalue=5e-42,
Organism=Escherichia coli, GI1789331, Length=193, Percent_Identity=23.3160621761658, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=183, Percent_Identity=34.9726775956284, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319304, Length=178, Percent_Identity=30.8988764044944, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324530, Length=184, Percent_Identity=26.6304347826087, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45550361, Length=174, Percent_Identity=32.7586206896552, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24782; Mature: 24782

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNKQTVLCIEEMAKMFPAAHCELVHKNTFELLVAVVLSAQCTDVLVNRVTASLFEKYH
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPEDYLAVPLEELMEDIRSIGLYRNKAKNIQGLSEKILIEFNGEVPKTHAELESLPGVGR
CCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCC
KTANVVLSVGFGIPAIAVDTHVERISKRLGICRWKDSVVEVEETLKRKLPKELWSDAHHY
HHHHEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEE
MIFFGRYHCKARNPECPTCPLLYLCREGKKQAKVRGFDG
EEEEEEEEECCCCCCCCCCHHHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure
MLSNKQTVLCIEEMAKMFPAAHCELVHKNTFELLVAVVLSAQCTDVLVNRVTASLFEKYH
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPEDYLAVPLEELMEDIRSIGLYRNKAKNIQGLSEKILIEFNGEVPKTHAELESLPGVGR
CCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCC
KTANVVLSVGFGIPAIAVDTHVERISKRLGICRWKDSVVEVEETLKRKLPKELWSDAHHY
HHHHEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHCCCCEE
MIFFGRYHCKARNPECPTCPLLYLCREGKKQAKVRGFDG
EEEEEEEEECCCCCCCCCCHHHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]