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The map label for this gene is pyrAa

Identifier: 226224439

GI number: 226224439

Start: 1899074

End: 1900165

Strand: Reverse

Name: pyrAa

Synonym: Lm4b_01852

Alternate gene names: 226224439

Gene position: 1900165-1899074 (Counterclockwise)

Preceding gene: 226224440

Following gene: 226224438

Centisome position: 65.24

GC content: 41.67

Gene sequence:

>1092_bases
ATGACAAAGCGAATTTTAATGCTAGAAGATGGCAATTATTTTATCGGTGATGCGATTGGTAGTGAAAAAGAAACCATTGG
TGAAGTTGTCTTCAATACTGGAATGACTGGCTACCAAGAAACAATCACTGACCCTTCGTACTATGGTCAAATTATTACTT
TTACGTATCCACTTGTTGGAAATTACGGTGTGAACCGCGACGATTTTGAATCTATCAATCCTGCTGTGAAAGGGGTTGTG
GTTCGTGAAGCTGCAGAATTTCCTTCCAACTGGCGCAATCAAATTACTTTAGATGAATTTTTGAAAGAAAAAGGCATCCC
GGGAATCGCGGGAATTGATACGCGTAAACTAACGAAACTAATTCGTAAAGAAGGAACGTTAAAAGGCATTTTAGCAGCTG
AAACTGCAAACAAAGAGGAATTGCTACACCACTTGCGCTCTGTACGTTTGCCAGTCGATCAAGTACATGAAGTTTCCTCT
GCTAAAGCATTTGCAAGCCCAGGAGATGGTAAACGAGTGGTGCTTGTTGATTATGGTGTGAAAAGTTCAATTTTACGGGA
ACTGAATAAACGTAACTGTTACGTGACGGTTGTTCCTTACAACACAACAGCCGAAGAAATTCTTGCAATGCATCCGGATG
GCGTGATGTTATCCAATGGACCTGGGGATCCGAAAGATGTGCCAGAAGCATTAGAAATGATACGCGGCATTCAAGGCAAG
CTCCCACTATTCGGAATTTGCTTAGGGCACCAACTGTTTGCACTTGCGAACGGAGCAGATACATTTAAACTGAAATTCGG
GCATCGTGGCGCGAATCATCCGGTGAAAGAACTAGCCACTGGACGTGTTGATTTCACTGCACAAAACCACGGTTACGCAG
TAGAAAAAGATTCACTTATTGGAACAGATTTAAAAGTAACACACATTGAATTAAATGATGAAACGGTAGAAGGGCTGGCA
CATAAAGAATATCCAGCCTATACAGTACAATATCACCCAGAAGCAAACCCAGGACCAAGTGACGTCAACTACTTATTTGA
TGAATTTATGGAAATGATGAATGGGAAAGAGGAGGGTGAACTACATGCCTAA

Upstream 100 bases:

>100_bases
CAAAAGGAAAAAACACACCATTTGTAGGAGAAACGTGTATTGGATGGCCAGTAGCAACGTTTTCGGAAGGCACACTTGTA
TACAATGAGGGGGAAATTAA

Downstream 100 bases:

>100_bases
ACGTGACGATATAAAAACAATTCTAGTAATCGGTTCTGGCCCAATTGTTATTGGACAAGCAGCAGAGTTCGATTATGCTG
GGACACAAGCATGCCTGAGT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTKRILMLEDGNYFIGDAIGSEKETIGEVVFNTGMTGYQETITDPSYYGQIITFTYPLVGNYGVNRDDFESINPAVKGVV
VREAAEFPSNWRNQITLDEFLKEKGIPGIAGIDTRKLTKLIRKEGTLKGILAAETANKEELLHHLRSVRLPVDQVHEVSS
AKAFASPGDGKRVVLVDYGVKSSILRELNKRNCYVTVVPYNTTAEEILAMHPDGVMLSNGPGDPKDVPEALEMIRGIQGK
LPLFGICLGHQLFALANGADTFKLKFGHRGANHPVKELATGRVDFTAQNHGYAVEKDSLIGTDLKVTHIELNDETVEGLA
HKEYPAYTVQYHPEANPGPSDVNYLFDEFMEMMNGKEEGELHA

Sequences:

>Translated_363_residues
MTKRILMLEDGNYFIGDAIGSEKETIGEVVFNTGMTGYQETITDPSYYGQIITFTYPLVGNYGVNRDDFESINPAVKGVV
VREAAEFPSNWRNQITLDEFLKEKGIPGIAGIDTRKLTKLIRKEGTLKGILAAETANKEELLHHLRSVRLPVDQVHEVSS
AKAFASPGDGKRVVLVDYGVKSSILRELNKRNCYVTVVPYNTTAEEILAMHPDGVMLSNGPGDPKDVPEALEMIRGIQGK
LPLFGICLGHQLFALANGADTFKLKFGHRGANHPVKELATGRVDFTAQNHGYAVEKDSLIGTDLKVTHIELNDETVEGLA
HKEYPAYTVQYHPEANPGPSDVNYLFDEFMEMMNGKEEGELHA
>Mature_362_residues
TKRILMLEDGNYFIGDAIGSEKETIGEVVFNTGMTGYQETITDPSYYGQIITFTYPLVGNYGVNRDDFESINPAVKGVVV
REAAEFPSNWRNQITLDEFLKEKGIPGIAGIDTRKLTKLIRKEGTLKGILAAETANKEELLHHLRSVRLPVDQVHEVSSA
KAFASPGDGKRVVLVDYGVKSSILRELNKRNCYVTVVPYNTTAEEILAMHPDGVMLSNGPGDPKDVPEALEMIRGIQGKL
PLFGICLGHQLFALANGADTFKLKFGHRGANHPVKELATGRVDFTAQNHGYAVEKDSLIGTDLKVTHIELNDETVEGLAH
KEYPAYTVQYHPEANPGPSDVNYLFDEFMEMMNGKEEGELHA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=366, Percent_Identity=40.9836065573771, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI21361331, Length=368, Percent_Identity=35.5978260869565, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI169790915, Length=368, Percent_Identity=35.5978260869565, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1786215, Length=372, Percent_Identity=45.6989247311828, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI1789760, Length=139, Percent_Identity=30.9352517985612, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=379, Percent_Identity=40.1055408970976, Blast_Score=262, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6324878, Length=369, Percent_Identity=39.5663956639566, Blast_Score=260, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=388, Percent_Identity=38.659793814433, Blast_Score=255, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6322638, Length=180, Percent_Identity=33.8888888888889, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45555749, Length=380, Percent_Identity=40.2631578947368, Blast_Score=251, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24642586, Length=380, Percent_Identity=40.2631578947368, Blast_Score=251, Evalue=5e-67,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40096; Mature: 39965

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRILMLEDGNYFIGDAIGSEKETIGEVVFNTGMTGYQETITDPSYYGQIITFTYPLVG
CCCEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHCCCHHHCCEEEEEEEECCC
NYGVNRDDFESINPAVKGVVVREAAEFPSNWRNQITLDEFLKEKGIPGIAGIDTRKLTKL
CCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHH
IRKEGTLKGILAAETANKEELLHHLRSVRLPVDQVHEVSSAKAFASPGDGKRVVLVDYGV
HHHCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
KSSILRELNKRNCYVTVVPYNTTAEEILAMHPDGVMLSNGPGDPKDVPEALEMIRGIQGK
HHHHHHHHHCCCEEEEEEECCCCHHHHHEECCCCEEECCCCCCHHHHHHHHHHHHCCCCC
LPLFGICLGHQLFALANGADTFKLKFGHRGANHPVKELATGRVDFTAQNHGYAVEKDSLI
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCCCEEEEECCCCEEEECCCCC
GTDLKVTHIELNDETVEGLAHKEYPAYTVQYHPEANPGPSDVNYLFDEFMEMMNGKEEGE
CCCEEEEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
LHA
CCC
>Mature Secondary Structure 
TKRILMLEDGNYFIGDAIGSEKETIGEVVFNTGMTGYQETITDPSYYGQIITFTYPLVG
CCEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHCCCHHHCCEEEEEEEECCC
NYGVNRDDFESINPAVKGVVVREAAEFPSNWRNQITLDEFLKEKGIPGIAGIDTRKLTKL
CCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHH
IRKEGTLKGILAAETANKEELLHHLRSVRLPVDQVHEVSSAKAFASPGDGKRVVLVDYGV
HHHCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCC
KSSILRELNKRNCYVTVVPYNTTAEEILAMHPDGVMLSNGPGDPKDVPEALEMIRGIQGK
HHHHHHHHHCCCEEEEEEECCCCHHHHHEECCCCEEECCCCCCHHHHHHHHHHHHCCCCC
LPLFGICLGHQLFALANGADTFKLKFGHRGANHPVKELATGRVDFTAQNHGYAVEKDSLI
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHCCCEEEEECCCCEEEECCCCC
GTDLKVTHIELNDETVEGLAHKEYPAYTVQYHPEANPGPSDVNYLFDEFMEMMNGKEEGE
CCCEEEEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCC
LHA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]