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The map label for this gene is trmD

Identifier: 226224395

GI number: 226224395

Start: 1851095

End: 1851832

Strand: Reverse

Name: trmD

Synonym: Lm4b_01808

Alternate gene names: 226224395

Gene position: 1851832-1851095 (Counterclockwise)

Preceding gene: 226224396

Following gene: 226224394

Centisome position: 63.58

GC content: 42.41

Gene sequence:

>738_bases
ATGAAAATTGACATTCTATCCATTTTCCCAGATATGTTTTCGGGAGTGACCGGGAATTCCATTATAAAAAAAGCCATCGA
AAACGAGCGTGTCGCGGTTGAAGTGACTGATTTCAGGGAATATGCAGAAGGAAAGCATCATATTGTCGATGATTACCCTT
ATGGTGGCGGGGCAGGTATGTTACTCAAAGCGCAACCGATATTTGATGCCGTTCAAGCTGTCAAAGAGAAACAACCAGAA
ACAAAGCCAAGAGTTATTTTAATGGACCCAGCAGGCAAACGCTTCGACCAAAAAATGGCAGAAGAATTTGCCGAAGAAGA
GCATCTTGTTTTTATTTGCGGGCACTATGAAGGTTACGACGAACGAATTCGCGAACACCTTGTTACGGATGAAGTGTCGA
TTGGAGATTACATTTTAACTGGTGGCGAAATTGGTGCAATGATTGTAATGGACAGTGTTATCCGACTTTTACCGGGAGTA
CTTGGTAATAAAGATTCCGCTGTGACAGATTCTTTCTCGACCGGTTTGCTTGAACATCCACACTACACGCGACCAGCCGA
TTTTAGAGGAATGAAAGTGCCAGATATTTTACTCAGCGGAAATCATGCATGGATAGAAGAATGGCGCGACAAAGAATCGC
TCAAAAGAACCTACGAACGCCGCCCAGATTTACTTAAAAACTATCCCTTAACAGACAAGCAAAAAACTTGGCTCAAAGAA
TGGTCTGACAGTAAATAA

Upstream 100 bases:

>100_bases
AAAAAGAAAAACTAATTCCTTATATTGCCGACGTTGTAAAAGAAATCAATATTAATGACAAAAAAATTACAATCGAAGTC
ATGGAAGGACTGCTAGATTA

Downstream 100 bases:

>100_bases
TCAAAGGACGTGATTTTAAATGGCAATTTTTGGAACACCTGATGAAAAAGAAACCAAAAAATCCGAGCGTCAAAAAGAAC
GAGAAGCCTATGTACAAGAA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKIDILSIFPDMFSGVTGNSIIKKAIENERVAVEVTDFREYAEGKHHIVDDYPYGGGAGMLLKAQPIFDAVQAVKEKQPE
TKPRVILMDPAGKRFDQKMAEEFAEEEHLVFICGHYEGYDERIREHLVTDEVSIGDYILTGGEIGAMIVMDSVIRLLPGV
LGNKDSAVTDSFSTGLLEHPHYTRPADFRGMKVPDILLSGNHAWIEEWRDKESLKRTYERRPDLLKNYPLTDKQKTWLKE
WSDSK

Sequences:

>Translated_245_residues
MKIDILSIFPDMFSGVTGNSIIKKAIENERVAVEVTDFREYAEGKHHIVDDYPYGGGAGMLLKAQPIFDAVQAVKEKQPE
TKPRVILMDPAGKRFDQKMAEEFAEEEHLVFICGHYEGYDERIREHLVTDEVSIGDYILTGGEIGAMIVMDSVIRLLPGV
LGNKDSAVTDSFSTGLLEHPHYTRPADFRGMKVPDILLSGNHAWIEEWRDKESLKRTYERRPDLLKNYPLTDKQKTWLKE
WSDSK
>Mature_245_residues
MKIDILSIFPDMFSGVTGNSIIKKAIENERVAVEVTDFREYAEGKHHIVDDYPYGGGAGMLLKAQPIFDAVQAVKEKQPE
TKPRVILMDPAGKRFDQKMAEEFAEEEHLVFICGHYEGYDERIREHLVTDEVSIGDYILTGGEIGAMIVMDSVIRLLPGV
LGNKDSAVTDSFSTGLLEHPHYTRPADFRGMKVPDILLSGNHAWIEEWRDKESLKRTYERRPDLLKNYPLTDKQKTWLKE
WSDSK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=46.8879668049792, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27929; Mature: 27929

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDILSIFPDMFSGVTGNSIIKKAIENERVAVEVTDFREYAEGKHHIVDDYPYGGGAGM
CEEEHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCEECCCCCCCCCCE
LLKAQPIFDAVQAVKEKQPETKPRVILMDPAGKRFDQKMAEEFAEEEHLVFICGHYEGYD
EEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHH
ERIREHLVTDEVSIGDYILTGGEIGAMIVMDSVIRLLPGVLGNKDSAVTDSFSTGLLEHP
HHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCCCC
HYTRPADFRGMKVPDILLSGNHAWIEEWRDKESLKRTYERRPDLLKNYPLTDKQKTWLKE
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHH
WSDSK
CCCCC
>Mature Secondary Structure
MKIDILSIFPDMFSGVTGNSIIKKAIENERVAVEVTDFREYAEGKHHIVDDYPYGGGAGM
CEEEHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCEECCCCCCCCCCE
LLKAQPIFDAVQAVKEKQPETKPRVILMDPAGKRFDQKMAEEFAEEEHLVFICGHYEGYD
EEECCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHH
ERIREHLVTDEVSIGDYILTGGEIGAMIVMDSVIRLLPGVLGNKDSAVTDSFSTGLLEHP
HHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCCCC
HYTRPADFRGMKVPDILLSGNHAWIEEWRDKESLKRTYERRPDLLKNYPLTDKQKTWLKE
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHHHH
WSDSK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]