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The map label for this gene is ygcA [C]

Identifier: 226224354

GI number: 226224354

Start: 1806563

End: 1807924

Strand: Reverse

Name: ygcA [C]

Synonym: Lm4b_01765

Alternate gene names: 226224354

Gene position: 1807924-1806563 (Counterclockwise)

Preceding gene: 226224355

Following gene: 226224353

Centisome position: 62.07

GC content: 38.99

Gene sequence:

>1362_bases
TTGGAAGCATCCCTTTTAAAAAAGAACCAATCCATTGAACTTACTATTGAAGATTTAACGCATGACGGCAGTGGGGTTGG
CAAAATTGACGGATACCCACTTTTCATTCCAAATACGTTACCTGGCGAAAAAGTGACAGCCAAAATAATAAAATTAAATA
AAAATTATGGCTTTGCTCGGATGGAGAATATTGAAACTGTTAGTGCTGATCGTGTAGAGCCTCCTTGTGCGGTTTATTCC
AAATGTGGCGGTTGTAGCTTGCAACACTTAAGCTACGATGGCCAGCTTGAATTCAAACGAAATCAAGTGGAAGAAACGAT
GAAACGTATTGGCAAACTAAACGTAGAAGTTCCTGAAACACTAGGCATGGAAAACCCGTGGCGCTACCGGAATAAATCAC
AAGTACCAGTAGGATTTGTTAATGGTAAGTTAACAGCAGGTTTTTACCAAAAAAGAAGCCATGCCATTATCGACATGTCC
ACTTGTTTAATTCATAATGAGCAAGGTGATTTTGCTGTTCAAAAAACACGTGAAATCCTAGCTAAATACGGAACAGAGCC
TTATGATGAACAAACAGGAAAAGGCGATATCCGCCATATTATGACAAGATTTGCGCATACAACTGGACAGTTAATGATTG
TCCTTGTAACAACAAAAGAACGTATGCCATTTAAAGAAGAAATCGTACGGGAATTGGTCGAACAATTGGAATTAACCTCG
ATTGTTCAAAATATTAATCCACATAAAACAAATGTGATTTTTGGAGATCGTACGAAAACACTTTGGGGAAAAGATATCAT
TGAGGATACGATTCATGGCATTCGATTTGCAATTTCGGCTCGTTCATTTTATCAAGTAAACCCAATCCAAACAGAAGTTT
TGTATCAACAAGCAATCGATGCGGCTGAGTTAACAGGAGAAGAAACAGTGATTGACGCCTATTGCGGCATTGGTTCTATT
TCTCTTTGTCTCGCGAAAAAAGCCAAGCATGTCTACGGGGTAGAAATTGTGGATCAAGCGATACAAGATGCTCGTGCAAA
TGCAGAACTCAATGAATTAGCGAATACTACTTTTGAGACAGGGAAAGCAGAAGAGGTTATTCCGGCGTGGTACAAAGCTG
GAATTGTAGCAGACGTCTTAGTTGTCGATCCACCACGAAAAGGTTGCGATGAAAAATTACTGGAAACAATTTTAGCAATG
AAACCGAAGAAAGTAGTGTATGTTTCTTGTAATCCAGGGACATTAGCCCGTGATATGAAAATCTTAACGGATGGCGGCTA
TGTAGCGAAAAAAGTTCAGCCTGTAGATATGTTTCCAATGACAACACATATAGAAGCTGTGACTGTTCTACATTTAAATT
AA

Upstream 100 bases:

>100_bases
ACACCATGCTTGTTTTTAAAGAGCAAGAAGAAGCAGTAATTAAAGAGCCAATCGGCATCGAATATATTATTAAGTAAAGC
AAAGAGAGGGGCAAGGGACT

Downstream 100 bases:

>100_bases
GTATACAAAAACCGCCATAATGGGATAAATGGCGGTTTTTGCTTTTCGAGTGTGTGGTTGACTAATAAATTAACAGACGA
GATTAATAAAAACCAAGTCC

Product: hypothetical RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MEASLLKKNQSIELTIEDLTHDGSGVGKIDGYPLFIPNTLPGEKVTAKIIKLNKNYGFARMENIETVSADRVEPPCAVYS
KCGGCSLQHLSYDGQLEFKRNQVEETMKRIGKLNVEVPETLGMENPWRYRNKSQVPVGFVNGKLTAGFYQKRSHAIIDMS
TCLIHNEQGDFAVQKTREILAKYGTEPYDEQTGKGDIRHIMTRFAHTTGQLMIVLVTTKERMPFKEEIVRELVEQLELTS
IVQNINPHKTNVIFGDRTKTLWGKDIIEDTIHGIRFAISARSFYQVNPIQTEVLYQQAIDAAELTGEETVIDAYCGIGSI
SLCLAKKAKHVYGVEIVDQAIQDARANAELNELANTTFETGKAEEVIPAWYKAGIVADVLVVDPPRKGCDEKLLETILAM
KPKKVVYVSCNPGTLARDMKILTDGGYVAKKVQPVDMFPMTTHIEAVTVLHLN

Sequences:

>Translated_453_residues
MEASLLKKNQSIELTIEDLTHDGSGVGKIDGYPLFIPNTLPGEKVTAKIIKLNKNYGFARMENIETVSADRVEPPCAVYS
KCGGCSLQHLSYDGQLEFKRNQVEETMKRIGKLNVEVPETLGMENPWRYRNKSQVPVGFVNGKLTAGFYQKRSHAIIDMS
TCLIHNEQGDFAVQKTREILAKYGTEPYDEQTGKGDIRHIMTRFAHTTGQLMIVLVTTKERMPFKEEIVRELVEQLELTS
IVQNINPHKTNVIFGDRTKTLWGKDIIEDTIHGIRFAISARSFYQVNPIQTEVLYQQAIDAAELTGEETVIDAYCGIGSI
SLCLAKKAKHVYGVEIVDQAIQDARANAELNELANTTFETGKAEEVIPAWYKAGIVADVLVVDPPRKGCDEKLLETILAM
KPKKVVYVSCNPGTLARDMKILTDGGYVAKKVQPVDMFPMTTHIEAVTVLHLN
>Mature_453_residues
MEASLLKKNQSIELTIEDLTHDGSGVGKIDGYPLFIPNTLPGEKVTAKIIKLNKNYGFARMENIETVSADRVEPPCAVYS
KCGGCSLQHLSYDGQLEFKRNQVEETMKRIGKLNVEVPETLGMENPWRYRNKSQVPVGFVNGKLTAGFYQKRSHAIIDMS
TCLIHNEQGDFAVQKTREILAKYGTEPYDEQTGKGDIRHIMTRFAHTTGQLMIVLVTTKERMPFKEEIVRELVEQLELTS
IVQNINPHKTNVIFGDRTKTLWGKDIIEDTIHGIRFAISARSFYQVNPIQTEVLYQQAIDAAELTGEETVIDAYCGIGSI
SLCLAKKAKHVYGVEIVDQAIQDARANAELNELANTTFETGKAEEVIPAWYKAGIVADVLVVDPPRKGCDEKLLETILAM
KPKKVVYVSCNPGTLARDMKILTDGGYVAKKVQPVDMFPMTTHIEAVTVLHLN

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=198, Percent_Identity=36.8686868686869, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI51173878, Length=198, Percent_Identity=36.8686868686869, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI269784680, Length=209, Percent_Identity=31.1004784688995, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI269784676, Length=209, Percent_Identity=31.1004784688995, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI20149688, Length=209, Percent_Identity=31.1004784688995, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI269784678, Length=209, Percent_Identity=31.1004784688995, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1789148, Length=448, Percent_Identity=30.1339285714286, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787083, Length=325, Percent_Identity=26.4615384615385, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790403, Length=239, Percent_Identity=27.1966527196653, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71997438, Length=163, Percent_Identity=30.6748466257669, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322909, Length=432, Percent_Identity=25.2314814814815, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24666579, Length=414, Percent_Identity=29.2270531400966, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 50577; Mature: 50577

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEASLLKKNQSIELTIEDLTHDGSGVGKIDGYPLFIPNTLPGEKVTAKIIKLNKNYGFAR
CCCCHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHEEEEEEEECCCCCHH
MENIETVSADRVEPPCAVYSKCGGCSLQHLSYDGQLEFKRNQVEETMKRIGKLNVEVPET
HCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCCHHH
LGMENPWRYRNKSQVPVGFVNGKLTAGFYQKRSHAIIDMSTCLIHNEQGDFAVQKTREIL
CCCCCCCCCCCCCCCCEEEECCEEEECHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHHH
AKYGTEPYDEQTGKGDIRHIMTRFAHTTGQLMIVLVTTKERMPFKEEIVRELVEQLELTS
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
IVQNINPHKTNVIFGDRTKTLWGKDIIEDTIHGIRFAISARSFYQVNPIQTEVLYQQAID
HHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCEEEECCHHHHHHHHHHCC
AAELTGEETVIDAYCGIGSISLCLAKKAKHVYGVEIVDQAIQDARANAELNELANTTFET
HHHCCCCCEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
GKAEEVIPAWYKAGIVADVLVVDPPRKGCDEKLLETILAMKPKKVVYVSCNPGTLARDMK
CCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
ILTDGGYVAKKVQPVDMFPMTTHIEAVTVLHLN
EEECCCEEEEEECCCCCCCCEECEEEEEEEEEC
>Mature Secondary Structure
MEASLLKKNQSIELTIEDLTHDGSGVGKIDGYPLFIPNTLPGEKVTAKIIKLNKNYGFAR
CCCCHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCHHEEEEEEEECCCCCHH
MENIETVSADRVEPPCAVYSKCGGCSLQHLSYDGQLEFKRNQVEETMKRIGKLNVEVPET
HCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCCCEEEEHHHHHHHHHHHHHCCCCCCHHH
LGMENPWRYRNKSQVPVGFVNGKLTAGFYQKRSHAIIDMSTCLIHNEQGDFAVQKTREIL
CCCCCCCCCCCCCCCCEEEECCEEEECHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHHH
AKYGTEPYDEQTGKGDIRHIMTRFAHTTGQLMIVLVTTKERMPFKEEIVRELVEQLELTS
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHH
IVQNINPHKTNVIFGDRTKTLWGKDIIEDTIHGIRFAISARSFYQVNPIQTEVLYQQAID
HHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHEEEEECCCEEEECCHHHHHHHHHHCC
AAELTGEETVIDAYCGIGSISLCLAKKAKHVYGVEIVDQAIQDARANAELNELANTTFET
HHHCCCCCEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
GKAEEVIPAWYKAGIVADVLVVDPPRKGCDEKLLETILAMKPKKVVYVSCNPGTLARDMK
CCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
ILTDGGYVAKKVQPVDMFPMTTHIEAVTVLHLN
EEECCCEEEEEECCCCCCCCEECEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]