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The map label for this gene is lysC [H]

Identifier: 226223835

GI number: 226223835

Start: 1242587

End: 1243801

Strand: Direct

Name: lysC [H]

Synonym: Lm4b_01240

Alternate gene names: 226223835

Gene position: 1242587-1243801 (Clockwise)

Preceding gene: 226223834

Following gene: 226223837

Centisome position: 42.66

GC content: 40.33

Gene sequence:

>1215_bases
TTGGGACTAGTCGTATTAAAATTTGGCGGAACATCAGTGAGCACGGTGGATAAAATCGGGAAAACAGCAGATCAAGCCAT
TTATGAAAAAAAACAAGGAAATAAAGTCATCGTCGTCGTTTCCGCAATGGGGAAATCAACCGACAAATTAGTCGCGATGG
CAGAAGAAATTAGCGAGGCACCAGATAAACGCGAAATGGATATGCTTCTTTCTACTGGTGAGCAAATCACAATTGCTTTA
CTTGCGATGACATTAAAAGAAAAAGGGCACGATGCTATATCCTATACAGGATGGCAAGCGGGAATTGAAACAGAAGCCGT
ACATAGTAATGCTAGAATTGCCGATATTGATAGCGCTCGTATTGAATCTGCGCTTGATGCGGGACAAATTGTTGTTGTAG
CAGGTTTTCAAGGTTTCACGACAGATGGTGAAATTACTACATTAGGACGAGGTGGATCAGACACAACCGCCGTCGCCATC
GCTTCTGCTCTAAATGCCGTAAAATGCGCGATTTGTACGGATGTAGTCGGTGTATTTACGACAGATCCACGCTACGTGAA
AAAAGCCCAAAAGTTAGAACAAATCACCTATGATGAAATGTTAGAATTAGCTAATCTCGGTGCCGGTGTTTTACATCCAC
GCTCCGTTGAATATGCCAAAAATTTCCGAATTCCGCTTGAAGTCAGAGCGAGCCACGAACAAGTACCAGGAACAATGATT
GAGGAGGATTTAACAATGGAAAATACCAAAGTAGTCCGTGGAATTGCTTTTGAAGACCAAATCACCCGTGTAACTATCCA
CTGGAAACAAAAAGAAGCAATGCGAGTTTCTAAAGTCTTTACAAAATTAGCAGAAAATAATATTGATGTAGATATTATTA
TCCAAGGAATTACTGGACTAGGTCACGGTAATCTATCCTTCACTATCAAAACAAGCGCACTCTTAGCCACACTAGCAGTT
TTAGAAGAAAGCAAAGAATTACTTCAAATTGAAAAACTAGAATCCGAACAAGATTTAGCGAAGGTGTCCATTGTTGGCTC
TGGAATGGTAAGTAATCCAGGTGTAGCTGCACAGATGTTTGAAGCTTTAACAGAAAATAATATTCCAATAAAAATGATTA
GCACATCAGAAATCAAAGTTTCCACTGTCGTACCAGCGCGCAAAATGGTCGAAGCAGCCCAAGTTTTACATGACCAATTT
GAACTCGATAAATAA

Upstream 100 bases:

>100_bases
TTAAATAGCTTTTACATATAATAAACTTCCCTTCAACACATAAATGCGCTAAAATATAAACATTGATAAAAAAGAACTAA
CAAGGCAGGAGGGAACGAGA

Downstream 100 bases:

>100_bases
AAATAAGCTTGTAGCATCAAAGAGGATTTCTCTTTGTTACTGCAAGCTTATTGTTTTAATAGCGTTCTTCGTTATCAATC
GTTTCTTTACGATCCCATTT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MGLVVLKFGGTSVSTVDKIGKTADQAIYEKKQGNKVIVVVSAMGKSTDKLVAMAEEISEAPDKREMDMLLSTGEQITIAL
LAMTLKEKGHDAISYTGWQAGIETEAVHSNARIADIDSARIESALDAGQIVVVAGFQGFTTDGEITTLGRGGSDTTAVAI
ASALNAVKCAICTDVVGVFTTDPRYVKKAQKLEQITYDEMLELANLGAGVLHPRSVEYAKNFRIPLEVRASHEQVPGTMI
EEDLTMENTKVVRGIAFEDQITRVTIHWKQKEAMRVSKVFTKLAENNIDVDIIIQGITGLGHGNLSFTIKTSALLATLAV
LEESKELLQIEKLESEQDLAKVSIVGSGMVSNPGVAAQMFEALTENNIPIKMISTSEIKVSTVVPARKMVEAAQVLHDQF
ELDK

Sequences:

>Translated_404_residues
MGLVVLKFGGTSVSTVDKIGKTADQAIYEKKQGNKVIVVVSAMGKSTDKLVAMAEEISEAPDKREMDMLLSTGEQITIAL
LAMTLKEKGHDAISYTGWQAGIETEAVHSNARIADIDSARIESALDAGQIVVVAGFQGFTTDGEITTLGRGGSDTTAVAI
ASALNAVKCAICTDVVGVFTTDPRYVKKAQKLEQITYDEMLELANLGAGVLHPRSVEYAKNFRIPLEVRASHEQVPGTMI
EEDLTMENTKVVRGIAFEDQITRVTIHWKQKEAMRVSKVFTKLAENNIDVDIIIQGITGLGHGNLSFTIKTSALLATLAV
LEESKELLQIEKLESEQDLAKVSIVGSGMVSNPGVAAQMFEALTENNIPIKMISTSEIKVSTVVPARKMVEAAQVLHDQF
ELDK
>Mature_403_residues
GLVVLKFGGTSVSTVDKIGKTADQAIYEKKQGNKVIVVVSAMGKSTDKLVAMAEEISEAPDKREMDMLLSTGEQITIALL
AMTLKEKGHDAISYTGWQAGIETEAVHSNARIADIDSARIESALDAGQIVVVAGFQGFTTDGEITTLGRGGSDTTAVAIA
SALNAVKCAICTDVVGVFTTDPRYVKKAQKLEQITYDEMLELANLGAGVLHPRSVEYAKNFRIPLEVRASHEQVPGTMIE
EDLTMENTKVVRGIAFEDQITRVTIHWKQKEAMRVSKVFTKLAENNIDVDIIIQGITGLGHGNLSFTIKTSALLATLAVL
EESKELLQIEKLESEQDLAKVSIVGSGMVSNPGVAAQMFEALTENNIPIKMISTSEIKVSTVVPARKMVEAAQVLHDQFE
LDK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=340, Percent_Identity=31.1764705882353, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1790455, Length=460, Percent_Identity=28.4782608695652, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1790376, Length=266, Percent_Identity=28.9473684210526, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320893, Length=376, Percent_Identity=28.1914893617021, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43607; Mature: 43475

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLVVLKFGGTSVSTVDKIGKTADQAIYEKKQGNKVIVVVSAMGKSTDKLVAMAEEISEA
CCEEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCC
PDKREMDMLLSTGEQITIALLAMTLKEKGHDAISYTGWQAGIETEAVHSNARIADIDSAR
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCEEEECCHHH
IESALDAGQIVVVAGFQGFTTDGEITTLGRGGSDTTAVAIASALNAVKCAICTDVVGVFT
HHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TDPRYVKKAQKLEQITYDEMLELANLGAGVLHPRSVEYAKNFRIPLEVRASHEQVPGTMI
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCHH
EEDLTMENTKVVRGIAFEDQITRVTIHWKQKEAMRVSKVFTKLAENNIDVDIIIQGITGL
HCCCCCCCCCEEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
GHGNLSFTIKTSALLATLAVLEESKELLQIEKLESEQDLAKVSIVGSGMVSNPGVAAQMF
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEECCCCCCCCHHHHHH
EALTENNIPIKMISTSEIKVSTVVPARKMVEAAQVLHDQFELDK
HHHHCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLVVLKFGGTSVSTVDKIGKTADQAIYEKKQGNKVIVVVSAMGKSTDKLVAMAEEISEA
CEEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCC
PDKREMDMLLSTGEQITIALLAMTLKEKGHDAISYTGWQAGIETEAVHSNARIADIDSAR
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHCCCCEEEECCHHH
IESALDAGQIVVVAGFQGFTTDGEITTLGRGGSDTTAVAIASALNAVKCAICTDVVGVFT
HHHHCCCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TDPRYVKKAQKLEQITYDEMLELANLGAGVLHPRSVEYAKNFRIPLEVRASHEQVPGTMI
CCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCHH
EEDLTMENTKVVRGIAFEDQITRVTIHWKQKEAMRVSKVFTKLAENNIDVDIIIQGITGL
HCCCCCCCCCEEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
GHGNLSFTIKTSALLATLAVLEESKELLQIEKLESEQDLAKVSIVGSGMVSNPGVAAQMF
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEECCCCCCCCHHHHHH
EALTENNIPIKMISTSEIKVSTVVPARKMVEAAQVLHDQFELDK
HHHHCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036830; 8969504; 9384377; 2837491; 2168395 [H]