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The map label for this gene is cbiP

Identifier: 226223808

GI number: 226223808

Start: 1211454

End: 1212989

Strand: Direct

Name: cbiP

Synonym: Lm4b_01212

Alternate gene names: 226223808

Gene position: 1211454-1212989 (Clockwise)

Preceding gene: 226223807

Following gene: 226223809

Centisome position: 41.59

GC content: 40.36

Gene sequence:

>1536_bases
ATGGTAAAGCAAATAATGATTCAAGGTACCGCTTCTGATGCCGGGAAAAGTGTACTGGTTGCAGGCCTATGCCGCCTATT
CAAAAATAAAGGAAAGCGAGTTGTTCCATTTAAATCTCAAAATATGTCACTCAATTCTTTCATAACGGCGACAGGAGATG
AAATGGGACGCGCACAAGTTTTTCAAGCAGAAGCAGCTGGTGTTTTTCCAGATGTCCGGATGAATCCCGTGTTACTTAAA
CCGACCAATGACAGGCAGTCGCAAGTGATTTTTATGGGTGCAATTTTAGATAATATGGATGCTGTGACGTACCACGATTT
CAAACAAACCCTTATCCCGAAAATCCAGGCAGTTTACCAGAGTTTAGCAGATGAAAATGATATTATTGTACTAGAAGGTG
CTGGAAGCCCAGCCGAAATCAACTTAAACGACCGAGATATCGTGAATATGGGCATGGCAAAAATGGTCGATGCCCCAGTT
GTCTTAGTTGCTGATATTGACAAAGGTGGCGTTTTTGCTTCGATATACGGCACCATTATGCTGTTAAATGAAGAAGAGCG
GGCTCGAATCAAAGGTGTTATTATCAATAAATTCCGTGGCGATGTAGCTCTTTTACAACCCGGAATTGATATGATTGAGG
AACTCACAAACGTCCCGGTTATCGGTGTGATTCCTTATGCCAATCTACAACTGGAAGAAGAGGACAGCGTTTCTCTAAGT
GGGAAAAATTACGTGCCGGATAGTAACGCACTACTTGATATCGCGATTATTTGTTTACCGAGGATTTCTAATTTCACAGA
TTTTCATATTCTGGAAATCCAGCCCGACATAAGTGTACGCTACATCCGTAATATAGCTGATTTTGGAAACCCAGATTTAG
TCATTATTCCTGGAAGTAAAAATACACTAGAAGATATGGCTTTCCTCGAAGAATCTGGACTTAAAAATGCCATTCAAAAC
TATGCTAAAAATGCCGGAAAAGTAATCGGGATTTGTGGTGGCTACCAAATGCTCGGTAAAAAAATGCTCGATCCGAATCA
AGTGGAGAGTAAACAATTAGAAATAGCCGGACTTGGTTTGCTAGATACCGAGACCATTTTTCTCGACCAAAAACGCACTA
CTCAAATTACAGGTGTGACGCATTCAGGTGAAGCAGTTGAAGGTTACGAAATCCATATGGGTGAGACGAAGCGAGGCGAG
AGCACTAGTCCATTTTGCGAAATTAAAGCGGTGAACGGAAACGAAGAAACCCACCAAGATGGCGCTATCTCTGTCAACAA
AAATATCATCGGCACATATATTCATGGTATTTTCGACAATGATGTTTTCTTAGGAAATCTATTTGACGAACTACTAACAG
GTAAAAACAAATCCGTTTATCCGCATGAAATCATTAATCTGAAAGAACACAAAGAACAAGAATACGACAAACTAGCGGCC
CTTTTAGAAGCAAATATTCAAATGGACCAACTAGAAAAAATCATGAAAGGAGAAAAAATATGCGTATCTACACAAAAACC
GGCGATAAAGGAATGA

Upstream 100 bases:

>100_bases
TAGTACAACCTTGGCTCATCAAATTACATCAACAAGCTGGCTATCCACTGTTCAAAAAAGAAGCCGATTTTTTCGCGCAT
ACGGGGAAGGTGACTAATTA

Downstream 100 bases:

>100_bases
CAAGAATTATCGGGGGTAGCAAAGTAAGTAAAGATAATATCCGTATCGATGCATATGGAACTTTGGATGAACTTAATTCG
CTGATTGGCTACACGATAAC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MVKQIMIQGTASDAGKSVLVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQVFQAEAAGVFPDVRMNPVLLK
PTNDRQSQVIFMGAILDNMDAVTYHDFKQTLIPKIQAVYQSLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPV
VLVADIDKGGVFASIYGTIMLLNEEERARIKGVIINKFRGDVALLQPGIDMIEELTNVPVIGVIPYANLQLEEEDSVSLS
GKNYVPDSNALLDIAIICLPRISNFTDFHILEIQPDISVRYIRNIADFGNPDLVIIPGSKNTLEDMAFLEESGLKNAIQN
YAKNAGKVIGICGGYQMLGKKMLDPNQVESKQLEIAGLGLLDTETIFLDQKRTTQITGVTHSGEAVEGYEIHMGETKRGE
STSPFCEIKAVNGNEETHQDGAISVNKNIIGTYIHGIFDNDVFLGNLFDELLTGKNKSVYPHEIINLKEHKEQEYDKLAA
LLEANIQMDQLEKIMKGEKICVSTQKPAIKE

Sequences:

>Translated_511_residues
MVKQIMIQGTASDAGKSVLVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQVFQAEAAGVFPDVRMNPVLLK
PTNDRQSQVIFMGAILDNMDAVTYHDFKQTLIPKIQAVYQSLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPV
VLVADIDKGGVFASIYGTIMLLNEEERARIKGVIINKFRGDVALLQPGIDMIEELTNVPVIGVIPYANLQLEEEDSVSLS
GKNYVPDSNALLDIAIICLPRISNFTDFHILEIQPDISVRYIRNIADFGNPDLVIIPGSKNTLEDMAFLEESGLKNAIQN
YAKNAGKVIGICGGYQMLGKKMLDPNQVESKQLEIAGLGLLDTETIFLDQKRTTQITGVTHSGEAVEGYEIHMGETKRGE
STSPFCEIKAVNGNEETHQDGAISVNKNIIGTYIHGIFDNDVFLGNLFDELLTGKNKSVYPHEIINLKEHKEQEYDKLAA
LLEANIQMDQLEKIMKGEKICVSTQKPAIKE
>Mature_511_residues
MVKQIMIQGTASDAGKSVLVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQVFQAEAAGVFPDVRMNPVLLK
PTNDRQSQVIFMGAILDNMDAVTYHDFKQTLIPKIQAVYQSLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPV
VLVADIDKGGVFASIYGTIMLLNEEERARIKGVIINKFRGDVALLQPGIDMIEELTNVPVIGVIPYANLQLEEEDSVSLS
GKNYVPDSNALLDIAIICLPRISNFTDFHILEIQPDISVRYIRNIADFGNPDLVIIPGSKNTLEDMAFLEESGLKNAIQN
YAKNAGKVIGICGGYQMLGKKMLDPNQVESKQLEIAGLGLLDTETIFLDQKRTTQITGVTHSGEAVEGYEIHMGETKRGE
STSPFCEIKAVNGNEETHQDGAISVNKNIIGTYIHGIFDNDVFLGNLFDELLTGKNKSVYPHEIINLKEHKEQEYDKLAA
LLEANIQMDQLEKIMKGEKICVSTQKPAIKE

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 56164; Mature: 56164

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKQIMIQGTASDAGKSVLVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQV
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCEE
FQAEAAGVFPDVRMNPVLLKPTNDRQSQVIFMGAILDNMDAVTYHDFKQTLIPKIQAVYQ
EEHHCCCCCCCCCCCCEEEECCCCCCCEEEEEEEHHCCCCCEEHHHHHHHHHHHHHHHHH
SLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPVVLVADIDKGGVFASIYGTIM
HHCCCCCEEEEECCCCCCEEECCCCHHHHHCHHHHCCCCEEEEEECCCCCEEEEEEEEEE
LLNEEERARIKGVIINKFRGDVALLQPGIDMIEELTNVPVIGVIPYANLQLEEEDSVSLS
EECCHHCCEEEEEEEECCCCCEEEECCCHHHHHHHHCCCEEEEECCCCEEECCCCCEEEC
GKNYVPDSNALLDIAIICLPRISNFTDFHILEIQPDISVRYIRNIADFGNPDLVIIPGSK
CCCCCCCCCCCEEEEEEEHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCC
NTLEDMAFLEESGLKNAIQNYAKNAGKVIGICGGYQMLGKKMLDPNQVESKQLEIAGLGL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCCEEEEEECCE
LDTETIFLDQKRTTQITGVTHSGEAVEGYEIHMGETKRGESTSPFCEIKAVNGNEETHQD
ECCCEEEECCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCC
GAISVNKNIIGTYIHGIFDNDVFLGNLFDELLTGKNKSVYPHEIINLKEHKEQEYDKLAA
CCEEECCCEEEEEEEEEECCCEEEHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHH
LLEANIQMDQLEKIMKGEKICVSTQKPAIKE
HHHCCCCHHHHHHHHCCCEEEEECCCCCCCC
>Mature Secondary Structure
MVKQIMIQGTASDAGKSVLVAGLCRLFKNKGKRVVPFKSQNMSLNSFITATGDEMGRAQV
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCCCCCCCEE
FQAEAAGVFPDVRMNPVLLKPTNDRQSQVIFMGAILDNMDAVTYHDFKQTLIPKIQAVYQ
EEHHCCCCCCCCCCCCEEEECCCCCCCEEEEEEEHHCCCCCEEHHHHHHHHHHHHHHHHH
SLADENDIIVLEGAGSPAEINLNDRDIVNMGMAKMVDAPVVLVADIDKGGVFASIYGTIM
HHCCCCCEEEEECCCCCCEEECCCCHHHHHCHHHHCCCCEEEEEECCCCCEEEEEEEEEE
LLNEEERARIKGVIINKFRGDVALLQPGIDMIEELTNVPVIGVIPYANLQLEEEDSVSLS
EECCHHCCEEEEEEEECCCCCEEEECCCHHHHHHHHCCCEEEEECCCCEEECCCCCEEEC
GKNYVPDSNALLDIAIICLPRISNFTDFHILEIQPDISVRYIRNIADFGNPDLVIIPGSK
CCCCCCCCCCCEEEEEEEHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEECCCC
NTLEDMAFLEESGLKNAIQNYAKNAGKVIGICGGYQMLGKKMLDPNQVESKQLEIAGLGL
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCCEEEEEECCE
LDTETIFLDQKRTTQITGVTHSGEAVEGYEIHMGETKRGESTSPFCEIKAVNGNEETHQD
ECCCEEEECCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCC
GAISVNKNIIGTYIHGIFDNDVFLGNLFDELLTGKNKSVYPHEIINLKEHKEQEYDKLAA
CCEEECCCEEEEEEEEEECCCEEEHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHH
LLEANIQMDQLEKIMKGEKICVSTQKPAIKE
HHHCCCCHHHHHHHHCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]