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The map label for this gene is adhE [H]

Identifier: 226223780

GI number: 226223780

Start: 1189452

End: 1190912

Strand: Direct

Name: adhE [H]

Synonym: Lm4b_01184

Alternate gene names: 226223780

Gene position: 1189452-1190912 (Clockwise)

Preceding gene: 226223779

Following gene: 226223781

Centisome position: 40.84

GC content: 39.97

Gene sequence:

>1461_bases
GTGGCACTAGAAGATAAAGATTTACGCTCAATCCAAGAAGTTCGTAACCTCATTGAATCAGCTAACAAAGCACAAAAAGA
ACTTGCTGCAATGAGCCAACAACAAATTGATACAATTGTAAAAGCAATAGCTGATGCCGGTTATGGTGCTCGCGAAAAAC
TTGCGAAAATGGCCCATGAAGAAACTGGTTTCGGAATTTGGCAAGACAAAGTAATCAAAAACGTCTTTGCGTCGAAACAT
GTTTACAATTACATCAAAGATATGAAAACCATTGGTATGTTAAAAGAAGATAACGAAGAGAAAGTAATGGAGGTTGCAGT
TCCACTTGGCGTAGTAGCAGGATTAATTCCTTCTACAAACCCTACATCCACTGTTATTTACAAAACACTTATTTCGATTA
AAGCCGGAAATAGTATCGTATTTTCTCCACATCCAAACGCATTAAAAGCAATTCTTGAAACAGTAAGAATCATTAGTGAA
GCAGCAGAAAAAGCTGGTTGTCCAAAAGGCGCTATCAGCTGTATGACTGTTCCAACCATCCAAGGAACAGATCAACTGAT
GAAACACAAAGATACAGCAGTTATCCTTGCAACAGGTGGATCTGCAATGGTAAAAGCGGCTTATTCATCTGGTACTCCAG
CAATTGGAGTTGGTCCAGGTAATGGCCCAGCATTTATCGAACGCAGTGCTAACATTCCTCGCGCAGTGAAACATATTCTT
GATTCCAAAACATTCGATAACGGAACAATTTGCGCATCTGAGCAATCTGTCGTTGTGGAACGTGTGAATAAAGAAGCTGT
CATTGCTGAATTTAGAAAACAAGGAGCACACTTCTTATCCGATGCAGAAGCTGTTCAACTTGGTAAATTCATCTTACGTC
CAAATGGTTCGATGAATCCAGCAATCGTAGGTAAAAGCGTGCAACATATCGCTAACCTTGCTGGTCTAACTGTTCCAGCT
GACGCAAGAGTACTTATCGCTGAAGAAACAAAAGTTGGCGCTAAAATCCCTTATTCAAGAGAAAAATTAGCTCCAATCTT
GGCGTTCTATACAGCCGAAACTTGGCAAGAAGCTTGTGAACTTAGCATGGATATTCTTTATCATGAAGGAGCTGGACATA
CTTTAATCATCCACTCTGAAGATAAAGAAATCATTCGCGAATTCGCACTGAAAAAACCAGTTTCCCGTCTCTTAGTTAAT
ACACCAGGAGCACTTGGTGGAATTGGCGCAACAACAAATCTTGTACCTGCTTTAACACTTGGTTGTGGGGCAGTTGGAGG
AAGTTCATCATCTGATAATATCGGACCTGAAAATCTTTTCAACATTCGTCGCATCGCTACTGGCGTTTTAGAGTTAGAAG
ATATTCGTAAAGAAGAAAACCAAGCAACATCTGAACTTCCGGTTGATGCAGACGCACTCATCCAAAGTTTAGTCGAAAAA
GTTTTAGCAGAATTAAAATAA

Upstream 100 bases:

>100_bases
CATTAAATAATGTGCCTATCCCAAAAAGCAAGATTAAATATGCGAACAAGGATAAACTTGTTCACGCACTAAAAGATATT
TATGGAAGGAGTGAAAACTA

Downstream 100 bases:

>100_bases
AAAATAAAACACTAATTGGAGGAATTTTAAAATGGCAAACGCAAACGCATTAGGTATGATCGAAACTAAAGGTTTAGTAG
GAGCAGTAGAGGCAGCAGAC

Product: acetaldehyde dehydrogenase / alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MALEDKDLRSIQEVRNLIESANKAQKELAAMSQQQIDTIVKAIADAGYGAREKLAKMAHEETGFGIWQDKVIKNVFASKH
VYNYIKDMKTIGMLKEDNEEKVMEVAVPLGVVAGLIPSTNPTSTVIYKTLISIKAGNSIVFSPHPNALKAILETVRIISE
AAEKAGCPKGAISCMTVPTIQGTDQLMKHKDTAVILATGGSAMVKAAYSSGTPAIGVGPGNGPAFIERSANIPRAVKHIL
DSKTFDNGTICASEQSVVVERVNKEAVIAEFRKQGAHFLSDAEAVQLGKFILRPNGSMNPAIVGKSVQHIANLAGLTVPA
DARVLIAEETKVGAKIPYSREKLAPILAFYTAETWQEACELSMDILYHEGAGHTLIIHSEDKEIIREFALKKPVSRLLVN
TPGALGGIGATTNLVPALTLGCGAVGGSSSSDNIGPENLFNIRRIATGVLELEDIRKEENQATSELPVDADALIQSLVEK
VLAELK

Sequences:

>Translated_486_residues
MALEDKDLRSIQEVRNLIESANKAQKELAAMSQQQIDTIVKAIADAGYGAREKLAKMAHEETGFGIWQDKVIKNVFASKH
VYNYIKDMKTIGMLKEDNEEKVMEVAVPLGVVAGLIPSTNPTSTVIYKTLISIKAGNSIVFSPHPNALKAILETVRIISE
AAEKAGCPKGAISCMTVPTIQGTDQLMKHKDTAVILATGGSAMVKAAYSSGTPAIGVGPGNGPAFIERSANIPRAVKHIL
DSKTFDNGTICASEQSVVVERVNKEAVIAEFRKQGAHFLSDAEAVQLGKFILRPNGSMNPAIVGKSVQHIANLAGLTVPA
DARVLIAEETKVGAKIPYSREKLAPILAFYTAETWQEACELSMDILYHEGAGHTLIIHSEDKEIIREFALKKPVSRLLVN
TPGALGGIGATTNLVPALTLGCGAVGGSSSSDNIGPENLFNIRRIATGVLELEDIRKEENQATSELPVDADALIQSLVEK
VLAELK
>Mature_485_residues
ALEDKDLRSIQEVRNLIESANKAQKELAAMSQQQIDTIVKAIADAGYGAREKLAKMAHEETGFGIWQDKVIKNVFASKHV
YNYIKDMKTIGMLKEDNEEKVMEVAVPLGVVAGLIPSTNPTSTVIYKTLISIKAGNSIVFSPHPNALKAILETVRIISEA
AEKAGCPKGAISCMTVPTIQGTDQLMKHKDTAVILATGGSAMVKAAYSSGTPAIGVGPGNGPAFIERSANIPRAVKHILD
SKTFDNGTICASEQSVVVERVNKEAVIAEFRKQGAHFLSDAEAVQLGKFILRPNGSMNPAIVGKSVQHIANLAGLTVPAD
ARVLIAEETKVGAKIPYSREKLAPILAFYTAETWQEACELSMDILYHEGAGHTLIIHSEDKEIIREFALKKPVSRLLVNT
PGALGGIGATTNLVPALTLGCGAVGGSSSSDNIGPENLFNIRRIATGVLELEDIRKEENQATSELPVDADALIQSLVEKV
LAELK

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=463, Percent_Identity=45.3563714902808, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788797, Length=377, Percent_Identity=31.0344827586207, Blast_Score=152, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 51932; Mature: 51801

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEDKDLRSIQEVRNLIESANKAQKELAAMSQQQIDTIVKAIADAGYGAREKLAKMAHE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ETGFGIWQDKVIKNVFASKHVYNYIKDMKTIGMLKEDNEEKVMEVAVPLGVVAGLIPSTN
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PTSTVIYKTLISIKAGNSIVFSPHPNALKAILETVRIISEAAEKAGCPKGAISCMTVPTI
CCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
QGTDQLMKHKDTAVILATGGSAMVKAAYSSGTPAIGVGPGNGPAFIERSANIPRAVKHIL
CCHHHHHHCCCCEEEEEECCCHHEEEHHCCCCCEEECCCCCCCHHEECCCCCHHHHHHHH
DSKTFDNGTICASEQSVVVERVNKEAVIAEFRKQGAHFLSDAEAVQLGKFILRPNGSMNP
CCCCCCCCCEECCCHHHHHHHHCHHHHHHHHHHCCHHHHCCHHHHHHHHHEECCCCCCCH
AIVGKSVQHIANLAGLTVPADARVLIAEETKVGAKIPYSREKLAPILAFYTAETWQEACE
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSMDILYHEGAGHTLIIHSEDKEIIREFALKKPVSRLLVNTPGALGGIGATTNLVPALTL
HHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
GCGAVGGSSSSDNIGPENLFNIRRIATGVLELEDIRKEENQATSELPVDADALIQSLVEK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VLAELK
HHHHCC
>Mature Secondary Structure 
ALEDKDLRSIQEVRNLIESANKAQKELAAMSQQQIDTIVKAIADAGYGAREKLAKMAHE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ETGFGIWQDKVIKNVFASKHVYNYIKDMKTIGMLKEDNEEKVMEVAVPLGVVAGLIPSTN
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
PTSTVIYKTLISIKAGNSIVFSPHPNALKAILETVRIISEAAEKAGCPKGAISCMTVPTI
CCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
QGTDQLMKHKDTAVILATGGSAMVKAAYSSGTPAIGVGPGNGPAFIERSANIPRAVKHIL
CCHHHHHHCCCCEEEEEECCCHHEEEHHCCCCCEEECCCCCCCHHEECCCCCHHHHHHHH
DSKTFDNGTICASEQSVVVERVNKEAVIAEFRKQGAHFLSDAEAVQLGKFILRPNGSMNP
CCCCCCCCCEECCCHHHHHHHHCHHHHHHHHHHCCHHHHCCHHHHHHHHHEECCCCCCCH
AIVGKSVQHIANLAGLTVPADARVLIAEETKVGAKIPYSREKLAPILAFYTAETWQEACE
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSMDILYHEGAGHTLIIHSEDKEIIREFALKKPVSRLLVNTPGALGGIGATTNLVPALTL
HHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
GCGAVGGSSSSDNIGPENLFNIRRIATGVLELEDIRKEENQATSELPVDADALIQSLVEK
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VLAELK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]