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The map label for this gene is guaA

Identifier: 226223707

GI number: 226223707

Start: 1124379

End: 1125935

Strand: Direct

Name: guaA

Synonym: Lm4b_01109

Alternate gene names: 226223707

Gene position: 1124379-1125935 (Clockwise)

Preceding gene: 226223706

Following gene: 226223708

Centisome position: 38.6

GC content: 42.97

Gene sequence:

>1557_bases
TTGTTTAAAATTATGAAAGACTTTACCGAGCAAGAGAAAATTATCGTTCTAGATTTTGGTAGTCAGTACAATCAACTAAT
TACGCGCCGCATTCGTGAATTCGGTGTGTATAGTGAATTACATCCGCATACTATTACAGTTGAAGAAATGAAAGCACTAA
ATCCAACGGGGATTATTTTTTCAGGAGGACCTAACAGTGTGTATGACGAAGATGCCTTCCGCGCTGACGAAAGAATCTTT
GACATGGGAATTCCGATTTTGGGAATTTGTTACGGCATGCAATTAATGACAACGCACTTCGGTGGCAAAGTAGAACGTGC
GAAAGACCGCGAATACGGGAAAGCGGACATTCACGTTGAGAAGCCAAACCGTTTATTTGCTGGACTACCAACCGATCAAG
TTGTTTGGATGAGTCACGGCGATTTAGTCGTTGAGGAGCCTGCTGGTTTTGAAGTAACAATTACTAGTAAATCTTGCCCA
ATTGCAGGTATTGCGGACGAAGAACGTTCACTATACGGCGTGCAATTCCACCCAGAAGTACGTCACTCAGTTTACGGCAA
CGAATTACTGAAGAATTTTGCATTAAATGTTTGTGGCTGTAAAGGCGACTGGACAATGGAGAACTTTAGTGAAGTAGAAA
TTGCTAAAATCCAAGAAATCGTAGGCGACAAAAAAGTCTTGCTTGCCCTTTCTGGCGGTGTAGATTCCTCTGTTGTTGGT
GTGTTAATTCATAAAGCAATCGGCGACCAACTAACTTGTATTTTCGTAGACCATGGCCTTCTTCGCAAAGGGGAAGCTGA
CCAAGTAATGGCAACATTACAAGGCGAATTCAACATGAACATCATCAAAGTAGATGCGAAAAAACGCTTCATGGACAAAC
TTGCTGGCGTTTCTGACCCAGAGCAAAAACGTAAAATCATCGGTAACGAATTCATTTACGTATTCGACGATGAAGCTAAC
AAACTAGACGGCGTAGAATTCCTAGCTCAAGGAACACTTTACACAGACATCATCGAAAGTGGTACAGCAACTGCCCAAAC
AATCAAGTCTCACCACAATGTAGGCGGCTTACCAGAAGATATGCAATTTAAACTAATCGAACCATTAAATACACTTTTCA
AAGATGAAGTTCGCGCACTAGGAACAGAACTTGGCATGCCTGACGCAATCGTTTGGCGCCAACCATTCCCAGGCCCAGGC
TTAGGAATCCGCGTTCTTGGCGAAATTACGGAAGAGAAATTAGAGATTGTCCGCGATTCTGATTACATCTTGCGTGAAGA
AATCAAAAACGCAGGTCTAGAACGCGAAATCTGGCAATACTTTACTGCTCTTCCAAATATCCGTAGCGTTGGTGTTATGG
GTGATGGCAGAACGTATGACCATACTGTGGTTGTTCGTGCAGTGACGAGTATTGATGGTATGACAGCGGATTGGGCACGT
ATTCCGTGGGATGTACTGGAGAAAATTTCGGTACGGATTGTGAATGAAGTGGATCACGTGAATCGTGTTGTTTATGATAT
TACGAGTAAACCACCAGCTACGGTTGAGTGGGAGTAA

Upstream 100 bases:

>100_bases
AAAATGAAAGAAGATGAAAAGGACAAGTGATTTGTCCTTTTCTTATATTATTGTGAAGTAACCGCGATTTTGCGGTTTCA
TTTTATAGATAAGGGTGGTT

Downstream 100 bases:

>100_bases
AAAGCTAACATAATAAGAATAGGAATATCAATAAATCAACGTTTTTCAACTTATGAAATGCTTGATTTTCATTAAAAATG
ATATGGTTTCCTGCCACAAA

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIFSGGPNSVYDEDAFRADERIF
DMGIPILGICYGMQLMTTHFGGKVERAKDREYGKADIHVEKPNRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTITSKSCP
IAGIADEERSLYGVQFHPEVRHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDPEQKRKIIGNEFIYVFDDEAN
KLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPG
LGIRVLGEITEEKLEIVRDSDYILREEIKNAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE

Sequences:

>Translated_518_residues
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIFSGGPNSVYDEDAFRADERIF
DMGIPILGICYGMQLMTTHFGGKVERAKDREYGKADIHVEKPNRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTITSKSCP
IAGIADEERSLYGVQFHPEVRHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDPEQKRKIIGNEFIYVFDDEAN
KLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPG
LGIRVLGEITEEKLEIVRDSDYILREEIKNAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE
>Mature_518_residues
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIFSGGPNSVYDEDAFRADERIF
DMGIPILGICYGMQLMTTHFGGKVERAKDREYGKADIHVEKPNRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTITSKSCP
IAGIADEERSLYGVQFHPEVRHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDPEQKRKIIGNEFIYVFDDEAN
KLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPG
LGIRVLGEITEEKLEIVRDSDYILREEIKNAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4504035, Length=546, Percent_Identity=38.4615384615385, Blast_Score=308, Evalue=6e-84,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=50.4761904761905, Blast_Score=550, Evalue=1e-157,
Organism=Escherichia coli, GI1789760, Length=154, Percent_Identity=39.6103896103896, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71992717, Length=566, Percent_Identity=37.6325088339223, Blast_Score=335, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI71992710, Length=566, Percent_Identity=37.6325088339223, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI133901714, Length=566, Percent_Identity=37.6325088339223, Blast_Score=334, Evalue=8e-92,
Organism=Saccharomyces cerevisiae, GI6323873, Length=516, Percent_Identity=54.4573643410853, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6322638, Length=186, Percent_Identity=31.7204301075269, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281365319, Length=547, Percent_Identity=36.1974405850091, Blast_Score=289, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 58071; Mature: 58071

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIF
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCEEEE
SGGPNSVYDEDAFRADERIFDMGIPILGICYGMQLMTTHFGGKVERAKDREYGKADIHVE
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
KPNRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTITSKSCPIAGIADEERSLYGVQFHPEV
CCCCEEECCCCCCEEEEECCCEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHH
RHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
HHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHH
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDP
HHHHHHCCCCEEEEEEECCCCCCCCHHHHHHEECCCCCEEEEEECHHHHHHHHHCCCCCH
EQKRKIIGNEFIYVFDDEANKLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPED
HHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
MQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPGLGIRVLGEITEEKLEIVRDS
CCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
DYILREEIKNAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
CHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEHHCCCCCCCHHC
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE
CCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEECC
>Mature Secondary Structure
MFKIMKDFTEQEKIIVLDFGSQYNQLITRRIREFGVYSELHPHTITVEEMKALNPTGIIF
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCCCEEEE
SGGPNSVYDEDAFRADERIFDMGIPILGICYGMQLMTTHFGGKVERAKDREYGKADIHVE
CCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
KPNRLFAGLPTDQVVWMSHGDLVVEEPAGFEVTITSKSCPIAGIADEERSLYGVQFHPEV
CCCCEEECCCCCCEEEEECCCEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCHH
RHSVYGNELLKNFALNVCGCKGDWTMENFSEVEIAKIQEIVGDKKVLLALSGGVDSSVVG
HHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHH
VLIHKAIGDQLTCIFVDHGLLRKGEADQVMATLQGEFNMNIIKVDAKKRFMDKLAGVSDP
HHHHHHCCCCEEEEEEECCCCCCCCHHHHHHEECCCCCEEEEEECHHHHHHHHHCCCCCH
EQKRKIIGNEFIYVFDDEANKLDGVEFLAQGTLYTDIIESGTATAQTIKSHHNVGGLPED
HHHHHHCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCC
MQFKLIEPLNTLFKDEVRALGTELGMPDAIVWRQPFPGPGLGIRVLGEITEEKLEIVRDS
CCEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
DYILREEIKNAGLEREIWQYFTALPNIRSVGVMGDGRTYDHTVVVRAVTSIDGMTADWAR
CHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEEHHCCCCCCCHHC
IPWDVLEKISVRIVNEVDHVNRVVYDITSKPPATVEWE
CCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]