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The map label for this gene is glpK1 [H]

Identifier: 226223652

GI number: 226223652

Start: 1064099

End: 1065562

Strand: Direct

Name: glpK1 [H]

Synonym: Lm4b_01054

Alternate gene names: 226223652

Gene position: 1064099-1065562 (Clockwise)

Preceding gene: 226223651

Following gene: 226223653

Centisome position: 36.53

GC content: 37.98

Gene sequence:

>1464_bases
ATGAAGAAACACTATCAAATCGCTATTGATCAAAGCACATCTGGAACGAAAGTATTATTGTTTAAAGCAGGTGAACTTGT
AGATCGTTTAGATAAAAAGCATCGACAGTTGTATCCGAAGAAAGGCTGGGTAGAGCATAATCCAATCGAAATTTGTCAAA
ATGTCCGTACATTAATTGTGGACATATTGGCAAAACATGATTTAATTCCAGCGGATATCGAGCGACTTGCTTTGACGAAT
CAACGGGAAACAATTGTAGCTTGGGATAAACAAACGGGCAAGCCACTTTATAATGCGATTGTATGGCAATGTAATCGAAC
AAAAGAACTTTGCGAAACATTAAAAAAAGCTGGTTATGAAAAAAGAATTAAACAGCTTACCGGATTGAAAATTGATTCTT
ATTTTTCTGCTTCTAAAATGAAGTGGTTATTGGAAAATAGTTCTGCGGTGCGGGATGCAGCAAGTCGTAATCAATTGGCA
TTTGGTACAATGGATGCTTGGTTGTTATTTTCGCTTACGGATGATAGTAATTATTTCACAGACCGAACAAACGCCAGCCG
CACACTCCTTTATGATATTGAAAAAAATGATTGGTCAGATGAGTTACTCGAACTTTTTGGAGTTAAACGTGCTTATTTAC
CAGAAGTGAAGCAATCTGCTGGAAGTTTTGGAAGTTATCAGGAGATACCGATTCATAGTGTGATGGCTGATTCAGAAGCG
GCACTTTATGGACAAGGCTGTGATGCATTTGGGGCAGTGAAGGCGACGCTTGGGACAGGATGCTCTGTTATGATGCAAAT
TGGTGAGAACAGATTACCTGAAAACGATGCTATTTTGACAACTCTTGCCTGGGATGTTGCAGGCGTGAACCAGTTTGCGT
TAGAAGGAATTATTCGTTCTTGTGGAGACACATTGGTTTTTTTATCCGAACAATTAGAACTATTTAATGATTACGATACA
GCTTGTAAGCGAGCATTTCAATTACCATGCAATGAGGGTGTTGTGCTAATTCCTGGACAATTAGGTTTAGGTTCGCCTTA
TTGGAATACAGATATACGAGCAGAGATTCTTGGTCTCACACGAGAACATACAAAATGGCATATTATTCGTGCCGGTTTTA
CAAGTATTGCATTTCAAATTAAGGCTGTTATTGACCAAATGGAGTTAATGACAGGTCAAAATATTAAGCGCTTACAAGTA
GACGGTGGATTAACAAAGCAACATGAGTTGATGCAATATTTAGCAGATGTTTTAAAAGCAGAAGTGGCAGTCAGTCCCGC
GGAAGAACTTTCGGCAATTGGTGCTATTGAAATTGCCGACAATAAACTACAAAATAAGAGAACGATAGCAAAATTGTACC
AACCAAATATCACTCATACACATGCTTTGGAAGATTTTCAAGAATGGGAAAAACAAGTGAAAAGCAGTATTCTAAAATTA
CAGAAGGTGAGGAATTTTAAATGA

Upstream 100 bases:

>100_bases
CTGCTATCGCTGGAACGAGCCAAGAAATCTTTGATTATTATGGCTTATCTGCTACTGGTATTGTTGCTACAGTTATGAAA
AACCTTGGAGACGAAAGAAG

Downstream 100 bases:

>100_bases
ATAATTCGGATCTTGCAAAAGAGGTTGTAAAGCTTGTTGGTGGAAAAGAAAACATTTTGAGTGTAATTCACTGTGTGACA
AGACTACGGTTTAAGTTACG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase 1; Glycerokinase 1; GK 1 [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKKHYQIAIDQSTSGTKVLLFKAGELVDRLDKKHRQLYPKKGWVEHNPIEICQNVRTLIVDILAKHDLIPADIERLALTN
QRETIVAWDKQTGKPLYNAIVWQCNRTKELCETLKKAGYEKRIKQLTGLKIDSYFSASKMKWLLENSSAVRDAASRNQLA
FGTMDAWLLFSLTDDSNYFTDRTNASRTLLYDIEKNDWSDELLELFGVKRAYLPEVKQSAGSFGSYQEIPIHSVMADSEA
ALYGQGCDAFGAVKATLGTGCSVMMQIGENRLPENDAILTTLAWDVAGVNQFALEGIIRSCGDTLVFLSEQLELFNDYDT
ACKRAFQLPCNEGVVLIPGQLGLGSPYWNTDIRAEILGLTREHTKWHIIRAGFTSIAFQIKAVIDQMELMTGQNIKRLQV
DGGLTKQHELMQYLADVLKAEVAVSPAEELSAIGAIEIADNKLQNKRTIAKLYQPNITHTHALEDFQEWEKQVKSSILKL
QKVRNFK

Sequences:

>Translated_487_residues
MKKHYQIAIDQSTSGTKVLLFKAGELVDRLDKKHRQLYPKKGWVEHNPIEICQNVRTLIVDILAKHDLIPADIERLALTN
QRETIVAWDKQTGKPLYNAIVWQCNRTKELCETLKKAGYEKRIKQLTGLKIDSYFSASKMKWLLENSSAVRDAASRNQLA
FGTMDAWLLFSLTDDSNYFTDRTNASRTLLYDIEKNDWSDELLELFGVKRAYLPEVKQSAGSFGSYQEIPIHSVMADSEA
ALYGQGCDAFGAVKATLGTGCSVMMQIGENRLPENDAILTTLAWDVAGVNQFALEGIIRSCGDTLVFLSEQLELFNDYDT
ACKRAFQLPCNEGVVLIPGQLGLGSPYWNTDIRAEILGLTREHTKWHIIRAGFTSIAFQIKAVIDQMELMTGQNIKRLQV
DGGLTKQHELMQYLADVLKAEVAVSPAEELSAIGAIEIADNKLQNKRTIAKLYQPNITHTHALEDFQEWEKQVKSSILKL
QKVRNFK
>Mature_487_residues
MKKHYQIAIDQSTSGTKVLLFKAGELVDRLDKKHRQLYPKKGWVEHNPIEICQNVRTLIVDILAKHDLIPADIERLALTN
QRETIVAWDKQTGKPLYNAIVWQCNRTKELCETLKKAGYEKRIKQLTGLKIDSYFSASKMKWLLENSSAVRDAASRNQLA
FGTMDAWLLFSLTDDSNYFTDRTNASRTLLYDIEKNDWSDELLELFGVKRAYLPEVKQSAGSFGSYQEIPIHSVMADSEA
ALYGQGCDAFGAVKATLGTGCSVMMQIGENRLPENDAILTTLAWDVAGVNQFALEGIIRSCGDTLVFLSEQLELFNDYDT
ACKRAFQLPCNEGVVLIPGQLGLGSPYWNTDIRAEILGLTREHTKWHIIRAGFTSIAFQIKAVIDQMELMTGQNIKRLQV
DGGLTKQHELMQYLADVLKAEVAVSPAEELSAIGAIEIADNKLQNKRTIAKLYQPNITHTHALEDFQEWEKQVKSSILKL
QKVRNFK

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=437, Percent_Identity=36.6132723112128, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI189409120, Length=437, Percent_Identity=36.6132723112128, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI42794763, Length=443, Percent_Identity=36.117381489842, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI41393575, Length=432, Percent_Identity=35.8796296296296, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI88196792, Length=505, Percent_Identity=26.7326732673267, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=38.3367139959432, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1789987, Length=492, Percent_Identity=24.1869918699187, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789168, Length=389, Percent_Identity=21.3367609254499, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535599, Length=443, Percent_Identity=36.3431151241535, Blast_Score=263, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI71986140, Length=451, Percent_Identity=23.7250554323725, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71986147, Length=262, Percent_Identity=27.4809160305344, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321755, Length=490, Percent_Identity=29.5918367346939, Blast_Score=216, Evalue=5e-57,
Organism=Drosophila melanogaster, GI17864214, Length=499, Percent_Identity=33.0661322645291, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI24655555, Length=478, Percent_Identity=31.3807531380753, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24655560, Length=478, Percent_Identity=31.3807531380753, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24655570, Length=478, Percent_Identity=31.3807531380753, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24655574, Length=478, Percent_Identity=31.3807531380753, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24655565, Length=478, Percent_Identity=31.3807531380753, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24654573, Length=430, Percent_Identity=34.1860465116279, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19922058, Length=495, Percent_Identity=27.2727272727273, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24652871, Length=422, Percent_Identity=28.436018957346, Blast_Score=169, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24656367, Length=455, Percent_Identity=26.8131868131868, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI21357867, Length=467, Percent_Identity=25.6959314775161, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24656372, Length=324, Percent_Identity=27.7777777777778, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI45552909, Length=292, Percent_Identity=27.0547945205479, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54924; Mature: 54924

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00933 FGGY_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHYQIAIDQSTSGTKVLLFKAGELVDRLDKKHRQLYPKKGWVEHNPIEICQNVRTLIV
CCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DILAKHDLIPADIERLALTNQRETIVAWDKQTGKPLYNAIVWQCNRTKELCETLKKAGYE
HHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHEECCCHHHHHHHHHHCCHH
KRIKQLTGLKIDSYFSASKMKWLLENSSAVRDAASRNQLAFGTMDAWLLFSLTDDSNYFT
HHHHHHHCCCCHHHHCHHHHHHHHCCCHHHHHHHCCCCEEEECCCEEEEEEEECCCCCCC
DRTNASRTLLYDIEKNDWSDELLELFGVKRAYLPEVKQSAGSFGSYQEIPIHSVMADSEA
CCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCC
ALYGQGCDAFGAVKATLGTGCSVMMQIGENRLPENDAILTTLAWDVAGVNQFALEGIIRS
CEECCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHH
CGDTLVFLSEQLELFNDYDTACKRAFQLPCNEGVVLIPGQLGLGSPYWNTDIRAEILGLT
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHCC
REHTKWHIIRAGFTSIAFQIKAVIDQMELMTGQNIKRLQVDGGLTKQHELMQYLADVLKA
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
EVAVSPAEELSAIGAIEIADNKLQNKRTIAKLYQPNITHTHALEDFQEWEKQVKSSILKL
HHCCCCHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
QKVRNFK
HHHHCCC
>Mature Secondary Structure
MKKHYQIAIDQSTSGTKVLLFKAGELVDRLDKKHRQLYPKKGWVEHNPIEICQNVRTLIV
CCCEEEEEEECCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
DILAKHDLIPADIERLALTNQRETIVAWDKQTGKPLYNAIVWQCNRTKELCETLKKAGYE
HHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHEECCCHHHHHHHHHHCCHH
KRIKQLTGLKIDSYFSASKMKWLLENSSAVRDAASRNQLAFGTMDAWLLFSLTDDSNYFT
HHHHHHHCCCCHHHHCHHHHHHHHCCCHHHHHHHCCCCEEEECCCEEEEEEEECCCCCCC
DRTNASRTLLYDIEKNDWSDELLELFGVKRAYLPEVKQSAGSFGSYQEIPIHSVMADSEA
CCCCCCEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCC
ALYGQGCDAFGAVKATLGTGCSVMMQIGENRLPENDAILTTLAWDVAGVNQFALEGIIRS
CEECCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEHHHHHCCHHHHHHHHHHH
CGDTLVFLSEQLELFNDYDTACKRAFQLPCNEGVVLIPGQLGLGSPYWNTDIRAEILGLT
CCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHCC
REHTKWHIIRAGFTSIAFQIKAVIDQMELMTGQNIKRLQVDGGLTKQHELMQYLADVLKA
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
EVAVSPAEELSAIGAIEIADNKLQNKRTIAKLYQPNITHTHALEDFQEWEKQVKSSILKL
HHCCCCHHHHHHCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
QKVRNFK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]