Click here to switch to the map view.

The map label for this gene is liaR [H]

Identifier: 226223640

GI number: 226223640

Start: 1052089

End: 1052727

Strand: Direct

Name: liaR [H]

Synonym: Lm4b_01042

Alternate gene names: 226223640

Gene position: 1052089-1052727 (Clockwise)

Preceding gene: 226223639

Following gene: 226223641

Centisome position: 36.12

GC content: 38.34

Gene sequence:

>639_bases
ATGATAAAAGTATTACTTGTAGATGATCATGAAATGGTACGTATAGGTGTCTCAGCTTATCTTTCTGTGCAAGATGACAT
GGAAGTGGTTGGCGAGGCAGAGAACGGTCGTGAAGGGGCTGATATGGCTCTAGAACTACGCCCAGATATTATTTTAATGG
ATTTAGTTATGGATGAAATGGACGGCATCGAAGCCACAAAAGAAATTATGCAAAACTGGAAAGAAGCAAAAATAATTATC
GTTACAAGTTTTATTGATGATGAAAAAGTATACCCGGCACTTGAAGCTGGAGCGAGCAGTTATATGCTTAAAACTTCAAC
AGCGAGCGAAATTGCAGATGCGATTCGAGCTACATACGGCGGAGACTCTGTTCTTGAACCAGAAGTAACTGGCAAAATGA
TGCAACGGCTAACCGCTAAACCAGAGAAAAACTTACATGATGATTTAACGAATCGTGAAAATGAAATCTTACTTTTAATC
GCGGAAGGTAAATCCAACCAAGAAATCGCAGACGAACTTTTCATCACTTTAAAAACGGTGAAGACGCATGTAAGTAATAT
TTTATCGAAATTAGATGTGCAAGATCGTACGCAAGCAGCAATCTATGCATTTAAACATGACTTAGTAGAAAAAAAGTAG

Upstream 100 bases:

>100_bases
CTGAATTCGGCGGCACCATCAAAATAATTAGTTTCCCAGGAAGAGGAACTAGTGTAGAAATAAAAATCCCACTTGTTGCG
AAAAAGGACGTGGAAAGCGA

Downstream 100 bases:

>100_bases
CAAGGAGGTAACGTACACATGAAAGAAGGATTTGCAGTCATCGGTCTTGGGCGATTCGGCGGAAGTATTTGTCGTTCACT
TGTCGAGCAAGGAATGGAAG

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEMDGIEATKEIMQNWKEAKIII
VTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYGGDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLI
AEGKSNQEIADELFITLKTVKTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK

Sequences:

>Translated_212_residues
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEMDGIEATKEIMQNWKEAKIII
VTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYGGDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLI
AEGKSNQEIADELFITLKTVKTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK
>Mature_212_residues
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEMDGIEATKEIMQNWKEAKIII
VTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYGGDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLI
AEGKSNQEIADELFITLKTVKTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=35.609756097561, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=30.952380952381, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787473, Length=204, Percent_Identity=36.7647058823529, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790102, Length=209, Percent_Identity=30.1435406698565, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1786747, Length=209, Percent_Identity=28.7081339712919, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788712, Length=190, Percent_Identity=27.3684210526316, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=36.5384615384615, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788550, Length=102, Percent_Identity=34.3137254901961, Blast_Score=64, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23672; Mature: 23672

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEM
CEEEEEECCCCEEEEEHHHEEEECCCHHHHCCCCCCCCCCCEEEEECCCEEEHHHHHHHH
DGIEATKEIMQNWKEAKIIIVTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYG
CCHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHCC
GDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLIAEGKSNQEIADELFITLKTV
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
KTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKVLLVDDHEMVRIGVSAYLSVQDDMEVVGEAENGREGADMALELRPDIILMDLVMDEM
CEEEEEECCCCEEEEEHHHEEEECCCHHHHCCCCCCCCCCCEEEEECCCEEEHHHHHHHH
DGIEATKEIMQNWKEAKIIIVTSFIDDEKVYPALEAGASSYMLKTSTASEIADAIRATYG
CCHHHHHHHHHHHHCCEEEEEEEECCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHHCC
GDSVLEPEVTGKMMQRLTAKPEKNLHDDLTNRENEILLLIAEGKSNQEIADELFITLKTV
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
KTHVSNILSKLDVQDRTQAAIYAFKHDLVEKK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]