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The map label for this gene is sigB

Identifier: 226223514

GI number: 226223514

Start: 931026

End: 931805

Strand: Direct

Name: sigB

Synonym: Lm4b_00913

Alternate gene names: 226223514

Gene position: 931026-931805 (Clockwise)

Preceding gene: 226223513

Following gene: 226223515

Centisome position: 31.96

GC content: 38.85

Gene sequence:

>780_bases
ATGCCAAAAGTATCTCAACCTGATAAAGAGGCGAAAGAAAAAGTCTATATATGGATTGCCGCTTACCAAGAAAATGGCGA
TCAAGACGCCCAATATAATTTAGTTGTTCATTACAAAAACCTAGTAGAGTCTATTGCTCGCAAATATTCCCAAGGTAAAT
CTTTTCATGAAGATTTAGTTCAAGTTGGAAATATTGGCTTACTTGGAGCTATTAGAAGATATGATGCGACGTTTGGGAAA
AGCTTTGAAGCATTTGCTGTACCAACCATTGTTGGCGAAATTAAACGATTTTTACGTGATAAAACATGGAGTGTGCATGT
GCCGCGCCGAATCAAAGAATTAGGTCCGAAAATTAAAAATGCCGTAGAAGAGCTGACGAGAGAACTACAAAGCTCGCCGC
AAATTAGCGACATTGCTGATTTCATCGGTGTCACGGAGGAAGAAGTTTTAGAAGCAATGGAAATGGGAAAAAGCTACCAA
GCGCTTTCTGTAGATCATTCGATTGAAGCTGATTCGGATGGAAGTACAATTACCTTATTAGATGTTGTTGGTGGTACGGA
TGATGGATTTGAACGTGTGAATCAACGTATGCTCTTAGAGAAAGTTCTCCCTGTTTTAGATGAGCGAGAACAGAAGATTT
TGCAATTTACATTTATTGAAAATCGCAGTCAAAAAGAAACGGGTGAACTACTCGATATATCACAAATGCATGTGTCTAGA
ATCCAACGTCAAGCTATTAAAAAACTTCGAGAGGCGTTGCAGAATGAGGAAGTGGAGTAA

Upstream 100 bases:

>100_bases
TTTTTAATTGAAACATTAATGGATGACGTGAAACTTTATTATGATGAAGGGGTTTCTGTCGTAATGACCAAATATATTAA
TGAAAAGCAGGTGGAGGAGA

Downstream 100 bases:

>100_bases
ATGAACAAGGCAGTTGAATCAAATAATTTATTTGTATTTCAACGTTCTAAAGCATTACAACAATACTGCGGGGATGTTTA
TTATACCCATGAAGACGAAA

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MPKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLVQVGNIGLLGAIRRYDATFGK
SFEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKNAVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQ
ALSVDHSIEADSDGSTITLLDVVGGTDDGFERVNQRMLLEKVLPVLDEREQKILQFTFIENRSQKETGELLDISQMHVSR
IQRQAIKKLREALQNEEVE

Sequences:

>Translated_259_residues
MPKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLVQVGNIGLLGAIRRYDATFGK
SFEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKNAVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQ
ALSVDHSIEADSDGSTITLLDVVGGTDDGFERVNQRMLLEKVLPVLDEREQKILQFTFIENRSQKETGELLDISQMHVSR
IQRQAIKKLREALQNEEVE
>Mature_258_residues
PKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLVQVGNIGLLGAIRRYDATFGKS
FEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKNAVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQA
LSVDHSIEADSDGSTITLLDVVGGTDDGFERVNQRMLLEKVLPVLDEREQKILQFTFIENRSQKETGELLDISQMHVSRI
QRQAIKKLREALQNEEVE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=200, Percent_Identity=29.5, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789098, Length=260, Percent_Identity=25.7692307692308, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=25.3333333333333, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789871, Length=254, Percent_Identity=29.5275590551181, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29510; Mature: 29379

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLV
CCCCCCCCHHHHCEEEEEEEEEECCCCCCCEEEHEEHHHHHHHHHHHHHHCCCHHHHHHH
QVGNIGLLGAIRRYDATFGKSFEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKN
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCHHHHH
AVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQALSVDHSIEADSDGSTITLL
HHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEE
DVVGGTDDGFERVNQRMLLEKVLPVLDEREQKILQFTFIENRSQKETGELLDISQMHVSR
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IQRQAIKKLREALQNEEVE
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PKVSQPDKEAKEKVYIWIAAYQENGDQDAQYNLVVHYKNLVESIARKYSQGKSFHEDLV
CCCCCCCHHHHCEEEEEEEEEECCCCCCCEEEHEEHHHHHHHHHHHHHHCCCHHHHHHH
QVGNIGLLGAIRRYDATFGKSFEAFAVPTIVGEIKRFLRDKTWSVHVPRRIKELGPKIKN
HHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCHHHHH
AVEELTRELQSSPQISDIADFIGVTEEEVLEAMEMGKSYQALSVDHSIEADSDGSTITLL
HHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCCEEEEE
DVVGGTDDGFERVNQRMLLEKVLPVLDEREQKILQFTFIENRSQKETGELLDISQMHVSR
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IQRQAIKKLREALQNEEVE
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]