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The map label for this gene is murF

Identifier: 226223474

GI number: 226223474

Start: 894203

End: 895576

Strand: Direct

Name: murF

Synonym: Lm4b_00872

Alternate gene names: 226223474

Gene position: 894203-895576 (Clockwise)

Preceding gene: 226223473

Following gene: 226223475

Centisome position: 30.7

GC content: 39.52

Gene sequence:

>1374_bases
TTGAAAAAAACAGTAGGCGAAGTAGTAGCAATGTTAGATAGTTCCATCCATGTTGATGCATTTCGCGATGTCGTCATTAC
TGGCGTTTGTTTTGATACAAGGCAAATAAAGTCAGGGGATTTATTTGTTCCATTTGTTGGGAATGTGCGCGATGGACATG
AATTTGTGAGTCAAGCACGAGAAATGGGTGCAGTGGCTACTTTCTGGCAAAAAGATGTACCCAATCCACCGATGGATTTC
CCGGTTATTTTAGTAGAAGATACTTTGCTCGCACTTCAAGAACTAGCGGCGAAATATATCCAACAAGTAAAGCCAAAAGT
TATTGCGATTACCGGAAGCAATGGTAAAACGACGACAAAAGATATTATGGCTGCGATTGTAGAAACGACGTATAAAGTTC
ATTATACTGGCGGGAATTTTAATAATCATATCGGCTTACCGTACACCATTTTAACGATGCCAGAAGATACCGAAGTTGCT
GTACTTGAAATGGGCATGAATCATCGTCATGAAATCGAAGTGCTTTCCAAAATTGCCAAACCCGATATCGCGATTATTAC
GAATATCGGCGAGGCGCATATTGAATATCTTGGTTCGCGTGAAGAAATTGCCAAAGCAAAACTGGAAATTACTGCCGGGC
TTAATCCAAGCGGGATTTTAATTTATCCGCACGAAGAAACTTTACTGCTAGGAAATATTAATGGCGATTTCAGACAGCTC
ACTTTTGGTAAGTCGGAAGCTGCGGAAATTTATCCGTTAGAAATTCGTGCGGAAGCGGAAGGCACTTCTTTTATAACTAA
TTGGGAGCCGGAGCTAGAAATTTTTGTCCCTATTATTGGTGAACATAATGTTTTCAATACAATGGCGGCGATGCTTGCGG
CAAGAGAAATTGGAATTGAAGGTGAAAAAATTCAATCTGCGCTGAAAAATATGGAACGTTCTAAAAGTCGTTTAGAATGG
ATTACAACGAAAAGTGGCGCGCGTATTTTGAATGACGCTTATAACTCTAGTCCAACAGCATTAAAAACGGTTTTAAAAAC
ATTTATGCATATGGATTCGAGCGGCAAACCTAAATATTTGGTCCTTGCTGATATGTTGGAACTTGGAGATTTGTCGACAA
AGTTACATCAAGAATCAGCCGAAGTATTAGAAAAGGGTATGATTGAGAAGATTTTCTTGTATGGTGAGGCGATGAAAGCT
TTTGGTGACATAGCGGAAGCCAAAATTGGTCAGGGAAAAGTTCATCATTTTAACACGAAAGAATCGCTTGAGACGGCGCT
TCTTTCAGAAATGAATGGAAATGAATGGATACTGGTCAAAGGTTCTTACGGAATGGGCTTGAAAGATGTGGTAGAAAATC
TTATTATTAAGTAA

Upstream 100 bases:

>100_bases
AGCCAAAAATGCACTTAAATATAAATTAGAAGACTAAAATAAACAAGAAAGCAGTTTTCATATGCGGAAGCTGCTTTTTT
ATGGAAGAGAGCGATTTAAT

Downstream 100 bases:

>100_bases
CGACTAAAATTGGACAGAACATATTACCGCTTAATTGGTAATAATTTGGACAGGAGGAAAGGCATAATGGTTGCTTGTTT
GTGTATTCATGGTTTTACTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diamin opimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKKTVGEVVAMLDSSIHVDAFRDVVITGVCFDTRQIKSGDLFVPFVGNVRDGHEFVSQAREMGAVATFWQKDVPNPPMDF
PVILVEDTLLALQELAAKYIQQVKPKVIAITGSNGKTTTKDIMAAIVETTYKVHYTGGNFNNHIGLPYTILTMPEDTEVA
VLEMGMNHRHEIEVLSKIAKPDIAIITNIGEAHIEYLGSREEIAKAKLEITAGLNPSGILIYPHEETLLLGNINGDFRQL
TFGKSEAAEIYPLEIRAEAEGTSFITNWEPELEIFVPIIGEHNVFNTMAAMLAAREIGIEGEKIQSALKNMERSKSRLEW
ITTKSGARILNDAYNSSPTALKTVLKTFMHMDSSGKPKYLVLADMLELGDLSTKLHQESAEVLEKGMIEKIFLYGEAMKA
FGDIAEAKIGQGKVHHFNTKESLETALLSEMNGNEWILVKGSYGMGLKDVVENLIIK

Sequences:

>Translated_457_residues
MKKTVGEVVAMLDSSIHVDAFRDVVITGVCFDTRQIKSGDLFVPFVGNVRDGHEFVSQAREMGAVATFWQKDVPNPPMDF
PVILVEDTLLALQELAAKYIQQVKPKVIAITGSNGKTTTKDIMAAIVETTYKVHYTGGNFNNHIGLPYTILTMPEDTEVA
VLEMGMNHRHEIEVLSKIAKPDIAIITNIGEAHIEYLGSREEIAKAKLEITAGLNPSGILIYPHEETLLLGNINGDFRQL
TFGKSEAAEIYPLEIRAEAEGTSFITNWEPELEIFVPIIGEHNVFNTMAAMLAAREIGIEGEKIQSALKNMERSKSRLEW
ITTKSGARILNDAYNSSPTALKTVLKTFMHMDSSGKPKYLVLADMLELGDLSTKLHQESAEVLEKGMIEKIFLYGEAMKA
FGDIAEAKIGQGKVHHFNTKESLETALLSEMNGNEWILVKGSYGMGLKDVVENLIIK
>Mature_457_residues
MKKTVGEVVAMLDSSIHVDAFRDVVITGVCFDTRQIKSGDLFVPFVGNVRDGHEFVSQAREMGAVATFWQKDVPNPPMDF
PVILVEDTLLALQELAAKYIQQVKPKVIAITGSNGKTTTKDIMAAIVETTYKVHYTGGNFNNHIGLPYTILTMPEDTEVA
VLEMGMNHRHEIEVLSKIAKPDIAIITNIGEAHIEYLGSREEIAKAKLEITAGLNPSGILIYPHEETLLLGNINGDFRQL
TFGKSEAAEIYPLEIRAEAEGTSFITNWEPELEIFVPIIGEHNVFNTMAAMLAAREIGIEGEKIQSALKNMERSKSRLEW
ITTKSGARILNDAYNSSPTALKTVLKTFMHMDSSGKPKYLVLADMLELGDLSTKLHQESAEVLEKGMIEKIFLYGEAMKA
FGDIAEAKIGQGKVHHFNTKESLETALLSEMNGNEWILVKGSYGMGLKDVVENLIIK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=440, Percent_Identity=31.8181818181818, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50526; Mature: 50526

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVGEVVAMLDSSIHVDAFRDVVITGVCFDTRQIKSGDLFVPFVGNVRDGHEFVSQAR
CCCHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHH
EMGAVATFWQKDVPNPPMDFPVILVEDTLLALQELAAKYIQQVKPKVIAITGSNGKTTTK
HHCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
DIMAAIVETTYKVHYTGGNFNNHIGLPYTILTMPEDTEVAVLEMGMNHRHEIEVLSKIAK
HHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHCC
PDIAIITNIGEAHIEYLGSREEIAKAKLEITAGLNPSGILIYPHEETLLLGNINGDFRQL
CCEEEEECCCHHHHHHCCCHHHHHHEEEEEEECCCCCCEEEEECCCEEEEECCCCCCEEE
TFGKSEAAEIYPLEIRAEAEGTSFITNWEPELEIFVPIIGEHNVFNTMAAMLAAREIGIE
ECCCCCCCEEEEEEEEECCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCC
GEKIQSALKNMERSKSRLEWITTKSGARILNDAYNSSPTALKTVLKTFMHMDSSGKPKYL
HHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEE
VLADMLELGDLSTKLHQESAEVLEKGMIEKIFLYGEAMKAFGDIAEAKIGQGKVHHFNTK
EEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCH
ESLETALLSEMNGNEWILVKGSYGMGLKDVVENLIIK
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MKKTVGEVVAMLDSSIHVDAFRDVVITGVCFDTRQIKSGDLFVPFVGNVRDGHEFVSQAR
CCCHHHHHHHHHCCCCCHHHHHHEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHH
EMGAVATFWQKDVPNPPMDFPVILVEDTLLALQELAAKYIQQVKPKVIAITGSNGKTTTK
HHCHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
DIMAAIVETTYKVHYTGGNFNNHIGLPYTILTMPEDTEVAVLEMGMNHRHEIEVLSKIAK
HHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHCC
PDIAIITNIGEAHIEYLGSREEIAKAKLEITAGLNPSGILIYPHEETLLLGNINGDFRQL
CCEEEEECCCHHHHHHCCCHHHHHHEEEEEEECCCCCCEEEEECCCEEEEECCCCCCEEE
TFGKSEAAEIYPLEIRAEAEGTSFITNWEPELEIFVPIIGEHNVFNTMAAMLAAREIGIE
ECCCCCCCEEEEEEEEECCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCC
GEKIQSALKNMERSKSRLEWITTKSGARILNDAYNSSPTALKTVLKTFMHMDSSGKPKYL
HHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEE
VLADMLELGDLSTKLHQESAEVLEKGMIEKIFLYGEAMKAFGDIAEAKIGQGKVHHFNTK
EEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCH
ESLETALLSEMNGNEWILVKGSYGMGLKDVVENLIIK
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]