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The map label for this gene is yjbJ [H]

Identifier: 226223348

GI number: 226223348

Start: 757021

End: 757689

Strand: Direct

Name: yjbJ [H]

Synonym: Lm4b_00745

Alternate gene names: 226223348

Gene position: 757021-757689 (Clockwise)

Preceding gene: 226223347

Following gene: 226223349

Centisome position: 25.99

GC content: 41.55

Gene sequence:

>669_bases
ATGAATCCAGTAGAACAAGTAATAAGTGCTAGACAAGCCGAGTTTCAAAGCGCGTTCGAAGCAGCGAAACAATCGGCTAC
CACTTTTGAAACAAAATTAAACGAACGCTTAAATGAAACCACAACTGCCAAAACAACAAGTGTACAAACTGTCACAGATG
CCGAACTTACGCGCGCTCGAGAAGCATATGAAGCATTAATCAATCAATCCGACACCAATTCTGCCGCGTCTGCTTCAAGC
ACAACAACCGAGACTCCCACTACATCAACAGGTGAATCAACTAACTGGAACAACTACCAAATCAAACCAATCAGTGCGGA
AAATGAAGGAAAATATAGTGATTTAATCAAAACTTCGGCCGCGAAATATGGCGTTCCAGAAGCACTCATCAAACGAGTCA
TCCAAGTAGAATCCAATTTTAACCCGAACGTTGTATCAAGTGCTGGTGCAACAGGTTTAATGCAGTTGATGTACGGCTCG
AATCGTACAGACCCAGCAACTAATATCGACGCTGGAACAAAACAATTGGCAGGTTATATTAAAAAATATGATGGCGATCT
CAAACTGGCATTAGCAGCTTATAATGCTGGGCCAGGAAATGTTCGCAAATATGGCGGCGTACCACCATTCAAAGAAACAC
AAAACTATTTAACGAAAATTATTGGGTAA

Upstream 100 bases:

>100_bases
TTCATCCATCAATTTTTAAAACAATTGCGGGATGAAAGTGTCACGCTTGAAGAAACGAGTAAGTTGATGGAAACGTTAGT
ATAACAAAGGAGTGCCGTGG

Downstream 100 bases:

>100_bases
GGGAGAATAAACCATGGGAAACTGGCAACAAAAGTGGGAATCATGGCGCGATATGACTGCAGCAATGCAACAAGAAATCA
AAATTGAAGCAGCCGAAAAA

Product: transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNPVEQVISARQAEFQSAFEAAKQSATTFETKLNERLNETTTAKTTSVQTVTDAELTRAREAYEALINQSDTNSAASASS
TTTETPTTSTGESTNWNNYQIKPISAENEGKYSDLIKTSAAKYGVPEALIKRVIQVESNFNPNVVSSAGATGLMQLMYGS
NRTDPATNIDAGTKQLAGYIKKYDGDLKLALAAYNAGPGNVRKYGGVPPFKETQNYLTKIIG

Sequences:

>Translated_222_residues
MNPVEQVISARQAEFQSAFEAAKQSATTFETKLNERLNETTTAKTTSVQTVTDAELTRAREAYEALINQSDTNSAASASS
TTTETPTTSTGESTNWNNYQIKPISAENEGKYSDLIKTSAAKYGVPEALIKRVIQVESNFNPNVVSSAGATGLMQLMYGS
NRTDPATNIDAGTKQLAGYIKKYDGDLKLALAAYNAGPGNVRKYGGVPPFKETQNYLTKIIG
>Mature_222_residues
MNPVEQVISARQAEFQSAFEAAKQSATTFETKLNERLNETTTAKTTSVQTVTDAELTRAREAYEALINQSDTNSAASASS
TTTETPTTSTGESTNWNNYQIKPISAENEGKYSDLIKTSAAKYGVPEALIKRVIQVESNFNPNVVSSAGATGLMQLMYGS
NRTDPATNIDAGTKQLAGYIKKYDGDLKLALAAYNAGPGNVRKYGGVPPFKETQNYLTKIIG

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=141, Percent_Identity=30.4964539007092, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082191, Length=118, Percent_Identity=34.7457627118644, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 23898; Mature: 23898

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVEQVISARQAEFQSAFEAAKQSATTFETKLNERLNETTTAKTTSVQTVTDAELTRAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
EAYEALINQSDTNSAASASSTTTETPTTSTGESTNWNNYQIKPISAENEGKYSDLIKTSA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
AKYGVPEALIKRVIQVESNFNPNVVSSAGATGLMQLMYGSNRTDPATNIDAGTKQLAGYI
HHCCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KKYDGDLKLALAAYNAGPGNVRKYGGVPPFKETQNYLTKIIG
HHCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNPVEQVISARQAEFQSAFEAAKQSATTFETKLNERLNETTTAKTTSVQTVTDAELTRAR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHH
EAYEALINQSDTNSAASASSTTTETPTTSTGESTNWNNYQIKPISAENEGKYSDLIKTSA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
AKYGVPEALIKRVIQVESNFNPNVVSSAGATGLMQLMYGSNRTDPATNIDAGTKQLAGYI
HHCCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
KKYDGDLKLALAAYNAGPGNVRKYGGVPPFKETQNYLTKIIG
HHCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]