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The map label for this gene is mdeA [H]

Identifier: 226223224

GI number: 226223224

Start: 640080

End: 641357

Strand: Reverse

Name: mdeA [H]

Synonym: Lm4b_00621

Alternate gene names: 226223224

Gene position: 641357-640080 (Counterclockwise)

Preceding gene: 226223226

Following gene: 226223223

Centisome position: 22.02

GC content: 42.02

Gene sequence:

>1278_bases
ATGAGTAATGAGTATAAATTCGAAACAATTCAAGTACACGGCGGACACACACCGGACGGAGATACGCATTCTAGAGCCGT
ACCAATTTATCAAACGACGTCATACACATTTGATAGCCCGGAACATGCCGCAGCCTTATTTGGTTTACAAGAAACTGGAA
ATATTTATACACGAATTATGAATCCGACTACAGCTGTTTTAGAAGAACGGTTAACGCTACTTGAAGGCGGTATTGGTGCG
GTTGCAACTGCTTCTGGGATGGCTGCTATTACTTACTCTATTTTAAATATTGCGGGTTCTGGAGATCATATCGTCGCAGC
CGCAACACTCTATGGCGGAACGCACACGCTATTTTCCCATACGTTTAAAACTTTTGGGATTGACGTAACGTTTGTTGACC
CTAATGAACCTGAAAATTTTGAAAAAGCGATTAAAGATAATACGAAAGCTGTTTTTATAGAAACAATTGGGAATCCTGAT
ATTAATATTGTCGATATCGAAAAAGTAGCAGAAATTGCGCACGCATCCGATATCCCGCTCATCGTTGACAACACATTCGC
GACCGCTTATTTGAATCGCCCGTTTGATTTTGGCGCAGATATCGTCGTTTACTCGGCAACAAAATTCATTGGTGGTCACG
GGGTTGCAATTGGTGGTGCGGTTATTGACTCAGGTAAATTTAACTGGGCAAATGGCAAATTTCCAAAGCTAGTGGTACCA
GATGATAGCTACAATGGTTTGTCTTATACAAATGACGTTGGCGCGGCGGCATATATTACCAAACTACGCGTCTCACTCCT
TCGTGATACTGGCGCAGCACTTTCTCCGTTCAACGCCTTCTTACTCATTTTAGGCCTAGAAACACTATCCTTACGCCTTG
AGCAACACGTGAAAAATGCGAAACAAGTTGCCAATTTCTTAAATGACCATCCAAAAGTAGCTTGGGTGAATTATCCAGGT
TTGAGCGACAACAAATACCACGAATTAGCCCAAAAATATTTACCAAAAGGACCTGGCTCCATTTTCACATTTGGAGTTAA
AGGTGGCTACGAGGCTGGTAAAAAAGTGATTGAATCCGTGGAATTATTCTCGCATTTAGCGAATGTTGGCGACGCAAAAT
CGCTTATCATCCATCCAGCTTCCACGACCCATCAACAACTAAGCGAAGAACAACAATTAACAGCTGGCGTGAAACCAGAG
TCCATTCGCCTATCTATCGGAATCGAAAATGCCGATGATATTATCCAAGATTTAACGAAAGCATTAGAGCAAATTTAG

Upstream 100 bases:

>100_bases
AAGTTTAGGAAACTCTATCCTCTCTGGCGGCTTATATACTGCTAGGGAGGTTTTTTGATGGAAATTACTGATAAATACAT
ATTAAAGAGGAGTGGATTTT

Downstream 100 bases:

>100_bases
GAGGAAGTGAAGAGAAGTGACCTTACAACAAAAAGAACTATTTCAAAAAAGTCCTCTTCTGCTTGAAAATGGAGAAACAT
TAAGCCCTGTTTTAGTCGGC

Product: hypothetical protein

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSNEYKFETIQVHGGHTPDGDTHSRAVPIYQTTSYTFDSPEHAAALFGLQETGNIYTRIMNPTTAVLEERLTLLEGGIGA
VATASGMAAITYSILNIAGSGDHIVAAATLYGGTHTLFSHTFKTFGIDVTFVDPNEPENFEKAIKDNTKAVFIETIGNPD
INIVDIEKVAEIAHASDIPLIVDNTFATAYLNRPFDFGADIVVYSATKFIGGHGVAIGGAVIDSGKFNWANGKFPKLVVP
DDSYNGLSYTNDVGAAAYITKLRVSLLRDTGAALSPFNAFLLILGLETLSLRLEQHVKNAKQVANFLNDHPKVAWVNYPG
LSDNKYHELAQKYLPKGPGSIFTFGVKGGYEAGKKVIESVELFSHLANVGDAKSLIIHPASTTHQQLSEEQQLTAGVKPE
SIRLSIGIENADDIIQDLTKALEQI

Sequences:

>Translated_425_residues
MSNEYKFETIQVHGGHTPDGDTHSRAVPIYQTTSYTFDSPEHAAALFGLQETGNIYTRIMNPTTAVLEERLTLLEGGIGA
VATASGMAAITYSILNIAGSGDHIVAAATLYGGTHTLFSHTFKTFGIDVTFVDPNEPENFEKAIKDNTKAVFIETIGNPD
INIVDIEKVAEIAHASDIPLIVDNTFATAYLNRPFDFGADIVVYSATKFIGGHGVAIGGAVIDSGKFNWANGKFPKLVVP
DDSYNGLSYTNDVGAAAYITKLRVSLLRDTGAALSPFNAFLLILGLETLSLRLEQHVKNAKQVANFLNDHPKVAWVNYPG
LSDNKYHELAQKYLPKGPGSIFTFGVKGGYEAGKKVIESVELFSHLANVGDAKSLIIHPASTTHQQLSEEQQLTAGVKPE
SIRLSIGIENADDIIQDLTKALEQI
>Mature_424_residues
SNEYKFETIQVHGGHTPDGDTHSRAVPIYQTTSYTFDSPEHAAALFGLQETGNIYTRIMNPTTAVLEERLTLLEGGIGAV
ATASGMAAITYSILNIAGSGDHIVAAATLYGGTHTLFSHTFKTFGIDVTFVDPNEPENFEKAIKDNTKAVFIETIGNPDI
NIVDIEKVAEIAHASDIPLIVDNTFATAYLNRPFDFGADIVVYSATKFIGGHGVAIGGAVIDSGKFNWANGKFPKLVVPD
DSYNGLSYTNDVGAAAYITKLRVSLLRDTGAALSPFNAFLLILGLETLSLRLEQHVKNAKQVANFLNDHPKVAWVNYPGL
SDNKYHELAQKYLPKGPGSIFTFGVKGGYEAGKKVIESVELFSHLANVGDAKSLIIHPASTTHQQLSEEQQLTAGVKPES
IRLSIGIENADDIIQDLTKALEQI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=405, Percent_Identity=34.8148148148148, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI299473759, Length=405, Percent_Identity=31.6049382716049, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI25453487, Length=404, Percent_Identity=29.7029702970297, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790375, Length=414, Percent_Identity=31.6425120772947, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789383, Length=433, Percent_Identity=26.7898383371824, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=431, Percent_Identity=34.1067285382831, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17538055, Length=421, Percent_Identity=33.0166270783848, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=421, Percent_Identity=33.2541567695962, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=48.6175115207373, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319307, Length=426, Percent_Identity=33.8028169014084, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321254, Length=443, Percent_Identity=27.5395033860045, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=26.9662921348315, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=429, Percent_Identity=25.8741258741259, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922554, Length=423, Percent_Identity=34.7517730496454, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45839; Mature: 45708

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEYKFETIQVHGGHTPDGDTHSRAVPIYQTTSYTFDSPEHAAALFGLQETGNIYTRIM
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCEEEEEC
NPTTAVLEERLTLLEGGIGAVATASGMAAITYSILNIAGSGDHIVAAATLYGGTHTLFSH
CCHHHHHHHHHHHHHCCCCHHHHHCCHHHHHEEHHHCCCCCCEEEEEEEECCCHHHHHHH
TFKTFGIDVTFVDPNEPENFEKAIKDNTKAVFIETIGNPDINIVDIEKVAEIAHASDIPL
HHHHHCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHCCCCCCE
IVDNTFATAYLNRPFDFGADIVVYSATKFIGGHGVAIGGAVIDSGKFNWANGKFPKLVVP
EEECCEEEEEECCCCCCCCCEEEEEEHHHCCCCCEEECCEEEECCCEECCCCCCCEEEEC
DDSYNGLSYTNDVGAAAYITKLRVSLLRDTGAALSPFNAFLLILGLETLSLRLEQHVKNA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQVANFLNDHPKVAWVNYPGLSDNKYHELAQKYLPKGPGSIFTFGVKGGYEAGKKVIESV
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
ELFSHLANVGDAKSLIIHPASTTHQQLSEEQQLTAGVKPESIRLSIGIENADDIIQDLTK
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
ALEQI
HHHCC
>Mature Secondary Structure 
SNEYKFETIQVHGGHTPDGDTHSRAVPIYQTTSYTFDSPEHAAALFGLQETGNIYTRIM
CCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCCCEEEEEC
NPTTAVLEERLTLLEGGIGAVATASGMAAITYSILNIAGSGDHIVAAATLYGGTHTLFSH
CCHHHHHHHHHHHHHCCCCHHHHHCCHHHHHEEHHHCCCCCCEEEEEEEECCCHHHHHHH
TFKTFGIDVTFVDPNEPENFEKAIKDNTKAVFIETIGNPDINIVDIEKVAEIAHASDIPL
HHHHHCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHCCCCCCE
IVDNTFATAYLNRPFDFGADIVVYSATKFIGGHGVAIGGAVIDSGKFNWANGKFPKLVVP
EEECCEEEEEECCCCCCCCCEEEEEEHHHCCCCCEEECCEEEECCCEECCCCCCCEEEEC
DDSYNGLSYTNDVGAAAYITKLRVSLLRDTGAALSPFNAFLLILGLETLSLRLEQHVKNA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQVANFLNDHPKVAWVNYPGLSDNKYHELAQKYLPKGPGSIFTFGVKGGYEAGKKVIESV
HHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
ELFSHLANVGDAKSLIIHPASTTHQQLSEEQQLTAGVKPESIRLSIGIENADDIIQDLTK
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHH
ALEQI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]