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The map label for this gene is ykvZ [H]

Identifier: 226223164

GI number: 226223164

Start: 576240

End: 577208

Strand: Reverse

Name: ykvZ [H]

Synonym: Lm4b_00561

Alternate gene names: 226223164

Gene position: 577208-576240 (Counterclockwise)

Preceding gene: 226223168

Following gene: 226223155

Centisome position: 19.82

GC content: 40.87

Gene sequence:

>969_bases
TTGGCAAATATACAAGAAATCGCGAAACTAGCGGGTGTTTCATCAGCAACGGTTTCCCGAGTGATTAATAAGCGCGACTA
TGTAAGCGATGAAACCAGAAAACGTGTTCAGACAATAATTGATCAGTTAGATTATGTTCCGAATATAAACGCCGTGTCTC
TGAAAAAAGGGGCAACGAAGCTAATTGGCATGGTTATTCCTAGCTTTACAGATTCTCTGAATGTATTTTTGAAAAGTTTT
ACGATGATAGCGCAAGAGCATGGTTATAACGTCACATTGTTTATGACGCGGATTGATCCAGATAAAGAACTAGAGGCCTT
AGAGATGCTGCGCCAAAAACAAATTGATGCGCTCGTCCTGGTCATTCGGTCAAATGATTGGAAAACGATTGAACATTACA
CCAAATATGGCCCGATTGTCACTTGGCAACGTGTCGAAAGTGAAAAAATCCCTTCTGTATTTATGAACCAATATGATGGA
TATACGCTTGCACTAGAACATTTATATGCAAAAGGATATCGAAAATTCGTGAATGTTTACGGGAATACGACCGGGCTCAA
TACGCAAAGTCGCATGCTTGCTTTTAAAGACTTTTGTGCGCGTTATGAGCTTGATCCGCATGAGTTTAGACAGTTTTACG
GGCAAGGTTCTCGGCAAGACGGGGAAAAGATTGCGCATTGGTTTGCGGAAACAGAGCATAAACCTGACGCCTTTTTGACA
CCGAATGATTATTTTGCGGCTGGGCTTTTGACGGAAGCAAGACGGCTTGGTTACAGTGTCCCGGAAGATTTTGCGATTTG
TGGGTTCGATAATATGGAAATCGCGCATTTGCTTGATATCACAACGATTCACTACCCGGTAAATTTGCAGGCTGAAAATG
CTTTTACGCTCATTATGAATCAATTATTTGGTAGCAATTTGCCACTGCTTGATTTAGATTTTCATTTAGTTGAACGAAAA
ACGACATAA

Upstream 100 bases:

>100_bases
ACAGGTCAAGCACTTATTTTAAATTTATCCAAACTGGCTATTTGGCGGGATTTTTTGGTATACTAATTAGAGAGCTACGA
GAAATCGAGGTGTTTTTTAA

Downstream 100 bases:

>100_bases
TAAAAGGTTTCCTCGCAAATTTGGAGGAAACCTTTTTTGTTAGTTTTTAAAAGAATGAATTGGTGCTGGGATTCGACCGC
CGCGAGCAATGAAGTCCACA

Product: transcription regulator LacI

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MANIQEIAKLAGVSSATVSRVINKRDYVSDETRKRVQTIIDQLDYVPNINAVSLKKGATKLIGMVIPSFTDSLNVFLKSF
TMIAQEHGYNVTLFMTRIDPDKELEALEMLRQKQIDALVLVIRSNDWKTIEHYTKYGPIVTWQRVESEKIPSVFMNQYDG
YTLALEHLYAKGYRKFVNVYGNTTGLNTQSRMLAFKDFCARYELDPHEFRQFYGQGSRQDGEKIAHWFAETEHKPDAFLT
PNDYFAAGLLTEARRLGYSVPEDFAICGFDNMEIAHLLDITTIHYPVNLQAENAFTLIMNQLFGSNLPLLDLDFHLVERK
TT

Sequences:

>Translated_322_residues
MANIQEIAKLAGVSSATVSRVINKRDYVSDETRKRVQTIIDQLDYVPNINAVSLKKGATKLIGMVIPSFTDSLNVFLKSF
TMIAQEHGYNVTLFMTRIDPDKELEALEMLRQKQIDALVLVIRSNDWKTIEHYTKYGPIVTWQRVESEKIPSVFMNQYDG
YTLALEHLYAKGYRKFVNVYGNTTGLNTQSRMLAFKDFCARYELDPHEFRQFYGQGSRQDGEKIAHWFAETEHKPDAFLT
PNDYFAAGLLTEARRLGYSVPEDFAICGFDNMEIAHLLDITTIHYPVNLQAENAFTLIMNQLFGSNLPLLDLDFHLVERK
TT
>Mature_321_residues
ANIQEIAKLAGVSSATVSRVINKRDYVSDETRKRVQTIIDQLDYVPNINAVSLKKGATKLIGMVIPSFTDSLNVFLKSFT
MIAQEHGYNVTLFMTRIDPDKELEALEMLRQKQIDALVLVIRSNDWKTIEHYTKYGPIVTWQRVESEKIPSVFMNQYDGY
TLALEHLYAKGYRKFVNVYGNTTGLNTQSRMLAFKDFCARYELDPHEFRQFYGQGSRQDGEKIAHWFAETEHKPDAFLTP
NDYFAAGLLTEARRLGYSVPEDFAICGFDNMEIAHLLDITTIHYPVNLQAENAFTLIMNQLFGSNLPLLDLDFHLVERKT
T

Specific function: Repressor That Binds To The Purf Operator And Coregulates Other Genes For De Novo Purine Nucleotide Synthesis. It Is Involved In Regulation Of Purb, Purc, Purek, Purhd, Purl, Purmn And Guaba Expression. Binds Hypoxanthine And Guanine As Inducers. [C]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=27.3529411764706, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=27.5080906148867, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=26.0479041916168, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788474, Length=318, Percent_Identity=27.0440251572327, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789202, Length=296, Percent_Identity=25.6756756756757, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786268, Length=303, Percent_Identity=28.3828382838284, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789068, Length=284, Percent_Identity=26.4084507042254, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=24.6951219512195, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI48994940, Length=320, Percent_Identity=23.75, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790715, Length=277, Percent_Identity=25.6317689530686, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790689, Length=253, Percent_Identity=26.4822134387352, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787580, Length=287, Percent_Identity=25.7839721254355, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36947; Mature: 36815

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIQEIAKLAGVSSATVSRVINKRDYVSDETRKRVQTIIDQLDYVPNINAVSLKKGATK
CCCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHH
LIGMVIPSFTDSLNVFLKSFTMIAQEHGYNVTLFMTRIDPDKELEALEMLRQKQIDALVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEEE
VIRSNDWKTIEHYTKYGPIVTWQRVESEKIPSVFMNQYDGYTLALEHLYAKGYRKFVNVY
EEECCCCHHHHHHHHCCCEEEEEHHCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GNTTGLNTQSRMLAFKDFCARYELDPHEFRQFYGQGSRQDGEKIAHWFAETEHKPDAFLT
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEC
PNDYFAAGLLTEARRLGYSVPEDFAICGFDNMEIAHLLDITTIHYPVNLQAENAFTLIMN
CCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHEEEEEEEEEEEECCCHHHHHHH
QLFGSNLPLLDLDFHLVERKTT
HHHCCCCCEEECCHHHHCCCCC
>Mature Secondary Structure 
ANIQEIAKLAGVSSATVSRVINKRDYVSDETRKRVQTIIDQLDYVPNINAVSLKKGATK
CCHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECHHHHH
LIGMVIPSFTDSLNVFLKSFTMIAQEHGYNVTLFMTRIDPDKELEALEMLRQKQIDALVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCEEEE
VIRSNDWKTIEHYTKYGPIVTWQRVESEKIPSVFMNQYDGYTLALEHLYAKGYRKFVNVY
EEECCCCHHHHHHHHCCCEEEEEHHCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GNTTGLNTQSRMLAFKDFCARYELDPHEFRQFYGQGSRQDGEKIAHWFAETEHKPDAFLT
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEC
PNDYFAAGLLTEARRLGYSVPEDFAICGFDNMEIAHLLDITTIHYPVNLQAENAFTLIMN
CCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHEEEEEEEEEEEECCCHHHHHHH
QLFGSNLPLLDLDFHLVERKTT
HHHCCCCCEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]