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The map label for this gene is nfrA1 [H]

Identifier: 226222931

GI number: 226222931

Start: 328304

End: 329041

Strand: Direct

Name: nfrA1 [H]

Synonym: Lm4b_00323

Alternate gene names: 226222931

Gene position: 328304-329041 (Clockwise)

Preceding gene: 226222929

Following gene: 226222932

Centisome position: 11.27

GC content: 32.66

Gene sequence:

>738_bases
ATGAATACAACCATTCAACAATTGGTTAAACATGTTTCAGTAAGAGAATTTAAAAATGAAAGATTATCAGATGAAATCAA
ACAACATTTATTAACAGCTGCGAGAAGTGCATCTAGTTCGCATTTTGTACAATCTTTTTCTATTTTAGAAATCACAGATG
AAAAACTAAGAAAGGAACTAGCTGAAATTACAAATAGTGCTTCTTATGTAAATCAAACGGGGACCTTTTATGTGTTTGTA
GGGGATTTATACCGACAATCAAAACTTTTACTGGAGAATAATAGAACTTTAGATGGGTTGAGAAATATGGAATCATTACT
CGTCTCGGTGATAGATGCTACAATTGCAGCTCAAAATATGGTTATTGCTGCTGAGTCTCTTGATTTAGGAATCTGTTATA
TCGGTGGAATAAGAAATGATATTGAAAAAGTAGCTGAATTGCTAAACTTACCACCATTTACGATACCTGTTTTCGGATTA
ACTGTAGGCGTTCCAGAATATAAAAATCAAGTAAAACCACGGCTATTACTAGAAAATCAAGTAGGAGAAAATCAATATCC
ACATGAACAATTTACGGATTTAAAAGCGTATGAGGAATTGACCAAAGATTATTATGCTTTACGTGAAAAAAATCAGCATC
AAACTTCATGGGGAGACAAGAATGTAGCGTTTTTTGAAGAAATACGAAGACCAGAAATAGCTGGCTTTTTGAAAAAACAA
GGGTTTACATTGGATTAA

Upstream 100 bases:

>100_bases
TCAAAAATAATGATACCGGCATATGATTTTCGCATTTCAAATGATAGCGCAGATAGATTATACTTTATTTACAATATAAA
TTCAATTTGGGGGTTATGTT

Downstream 100 bases:

>100_bases
TTAATTTTCGTTGTAGGGCGTATAAAAAATAATAGTATTAAATTTATGAGAGGCTGGAAGAGTATTTTTTCCGGCTTTTT
ATTTGATTGGGGCTATTATT

Product: FMN-containing NADPH-linked nitro/flavin reductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNTTIQQLVKHVSVREFKNERLSDEIKQHLLTAARSASSSHFVQSFSILEITDEKLRKELAEITNSASYVNQTGTFYVFV
GDLYRQSKLLLENNRTLDGLRNMESLLVSVIDATIAAQNMVIAAESLDLGICYIGGIRNDIEKVAELLNLPPFTIPVFGL
TVGVPEYKNQVKPRLLLENQVGENQYPHEQFTDLKAYEELTKDYYALREKNQHQTSWGDKNVAFFEEIRRPEIAGFLKKQ
GFTLD

Sequences:

>Translated_245_residues
MNTTIQQLVKHVSVREFKNERLSDEIKQHLLTAARSASSSHFVQSFSILEITDEKLRKELAEITNSASYVNQTGTFYVFV
GDLYRQSKLLLENNRTLDGLRNMESLLVSVIDATIAAQNMVIAAESLDLGICYIGGIRNDIEKVAELLNLPPFTIPVFGL
TVGVPEYKNQVKPRLLLENQVGENQYPHEQFTDLKAYEELTKDYYALREKNQHQTSWGDKNVAFFEEIRRPEIAGFLKKQ
GFTLD
>Mature_245_residues
MNTTIQQLVKHVSVREFKNERLSDEIKQHLLTAARSASSSHFVQSFSILEITDEKLRKELAEITNSASYVNQTGTFYVFV
GDLYRQSKLLLENNRTLDGLRNMESLLVSVIDATIAAQNMVIAAESLDLGICYIGGIRNDIEKVAELLNLPPFTIPVFGL
TVGVPEYKNQVKPRLLLENQVGENQYPHEQFTDLKAYEELTKDYYALREKNQHQTSWGDKNVAFFEEIRRPEIAGFLKKQ
GFTLD

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=243, Percent_Identity=38.2716049382716, Blast_Score=154, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 27948; Mature: 27948

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTIQQLVKHVSVREFKNERLSDEIKQHLLTAARSASSSHFVQSFSILEITDEKLRKEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AEITNSASYVNQTGTFYVFVGDLYRQSKLLLENNRTLDGLRNMESLLVSVIDATIAAQNM
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIAAESLDLGICYIGGIRNDIEKVAELLNLPPFTIPVFGLTVGVPEYKNQVKPRLLLENQ
EEEECCCCEEEEEECCHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHHCCCCHHEECCC
VGENQYPHEQFTDLKAYEELTKDYYALREKNQHQTSWGDKNVAFFEEIRRPEIAGFLKKQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHC
GFTLD
CCCCC
>Mature Secondary Structure
MNTTIQQLVKHVSVREFKNERLSDEIKQHLLTAARSASSSHFVQSFSILEITDEKLRKEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AEITNSASYVNQTGTFYVFVGDLYRQSKLLLENNRTLDGLRNMESLLVSVIDATIAAQNM
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIAAESLDLGICYIGGIRNDIEKVAELLNLPPFTIPVFGLTVGVPEYKNQVKPRLLLENQ
EEEECCCCEEEEEECCHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHHCCCCHHEECCC
VGENQYPHEQFTDLKAYEELTKDYYALREKNQHQTSWGDKNVAFFEEIRRPEIAGFLKKQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHC
GFTLD
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]