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The map label for this gene is dapE [H]

Identifier: 226222900

GI number: 226222900

Start: 294473

End: 295612

Strand: Direct

Name: dapE [H]

Synonym: Lm4b_00292

Alternate gene names: 226222900

Gene position: 294473-295612 (Clockwise)

Preceding gene: 226222899

Following gene: 226222901

Centisome position: 10.11

GC content: 38.33

Gene sequence:

>1140_bases
ATGGAACGACAAAGAAAAATTCAAATCTTAAAAGACATTGTGAATATTGATTCGACTAATGGGCATGAAGAACAAGTTGC
GAACTATTTGCAAAAGTTGTTAGCTGAACACGGTATTGAATCCGAAAAGGTACAATACGACGTAGACAGAGCCAGCCTAG
TTAGCGAAATTGGTTCCAGTGACGAGAAAGTTTTGGCGTTTTCAGGGCATATGGATGTCGTTGATGCGGGTGATGTCTCG
AAGTGGAAGTTCCCACCTTTTGAAGCAGCAGAGCATGAAGGGAAAATATACGGACGTGGCGCGACGGATATGAAGTCAGG
TCTAGCGGCGATGATTATTGCAATGATTGAGCTTCATGAAGAAAAACAAAAACTAAATGGCAAAATTAGATTATTAGCAA
CGGTTGGTGAAGAAGTCGGTGAACTTGGAGCCGAACAACTAACGCAAAAAGGTTACGCAGATGATTTAGATGGCTTGATT
ATCGGCGAACCGAGTGGACACCGGATTGTTTATGCGCATAAAGGTTCCATTAATTATACCGTTAAATCCACTGGTAAAAA
TGCGCATAGTTCGATGCCGGAATTCGGTGTTAATGCGATTGACAACTTGTTGCTATTTTATAATGAAGTAGAAAAATTCG
TGAAATCAATTGATGCTACTAACGAAATATTAGGCGATTTTATTCATAATGTCACCGTAATTGATGGTGGAAATCAAGTC
AATAGTATCCCTGAAAAAGCGCAACTAAAAGGGAATATTCGCTCGATTCCAGAAATGGATAACGAAACAGTGAAACAAGT
GCTAGTGAAGATTATCAATAAGTTAAATAAACAGGAAAATATGAATCTAGAATTAATATTTGATTATGACAAACAACCAG
TATTTAGTGATAAAAATTCGGATTTAGTCCACATTGCTAAGAGCGTAGCAAGCGACATTGTCAAAGAAGAAATCCCATTA
CTCGGTATTTCCGGAACAACCGATGCAGCAGAATTTACCAAAGCTAAGAAAGAGTTCCCAGTGATTATTTTTGGACCAGG
AAACGAAACCCCTCACCAAGTAAACGAAAATGTTTCTATAGAGAATTATTTGGAGATGGTAGATGTTTACAAACGAATTG
CCACCGAGTTTTTATCGTGA

Upstream 100 bases:

>100_bases
AGGAGCTAAGCCATTTTTTGATTGGAGATTAGGAGGATGAGTTTGCCTTTTTATGGAACGGGAAAACATAGAGTGGAATT
CAAAGAAAGAGGGCGTAAAC

Downstream 100 bases:

>100_bases
TGGAACTTTAACTTTACTTATTTTCCGCTATAAAATACGTAATTAAATAAAAGTCTAGTATTTGTTTGTAAACAGGTGCT
AGGCTTTTTTCTTGCTTTTA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase [H]

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MERQRKIQILKDIVNIDSTNGHEEQVANYLQKLLAEHGIESEKVQYDVDRASLVSEIGSSDEKVLAFSGHMDVVDAGDVS
KWKFPPFEAAEHEGKIYGRGATDMKSGLAAMIIAMIELHEEKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLI
IGEPSGHRIVYAHKGSINYTVKSTGKNAHSSMPEFGVNAIDNLLLFYNEVEKFVKSIDATNEILGDFIHNVTVIDGGNQV
NSIPEKAQLKGNIRSIPEMDNETVKQVLVKIINKLNKQENMNLELIFDYDKQPVFSDKNSDLVHIAKSVASDIVKEEIPL
LGISGTTDAAEFTKAKKEFPVIIFGPGNETPHQVNENVSIENYLEMVDVYKRIATEFLS

Sequences:

>Translated_379_residues
MERQRKIQILKDIVNIDSTNGHEEQVANYLQKLLAEHGIESEKVQYDVDRASLVSEIGSSDEKVLAFSGHMDVVDAGDVS
KWKFPPFEAAEHEGKIYGRGATDMKSGLAAMIIAMIELHEEKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLI
IGEPSGHRIVYAHKGSINYTVKSTGKNAHSSMPEFGVNAIDNLLLFYNEVEKFVKSIDATNEILGDFIHNVTVIDGGNQV
NSIPEKAQLKGNIRSIPEMDNETVKQVLVKIINKLNKQENMNLELIFDYDKQPVFSDKNSDLVHIAKSVASDIVKEEIPL
LGISGTTDAAEFTKAKKEFPVIIFGPGNETPHQVNENVSIENYLEMVDVYKRIATEFLS
>Mature_379_residues
MERQRKIQILKDIVNIDSTNGHEEQVANYLQKLLAEHGIESEKVQYDVDRASLVSEIGSSDEKVLAFSGHMDVVDAGDVS
KWKFPPFEAAEHEGKIYGRGATDMKSGLAAMIIAMIELHEEKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLI
IGEPSGHRIVYAHKGSINYTVKSTGKNAHSSMPEFGVNAIDNLLLFYNEVEKFVKSIDATNEILGDFIHNVTVIDGGNQV
NSIPEKAQLKGNIRSIPEMDNETVKQVLVKIINKLNKQENMNLELIFDYDKQPVFSDKNSDLVHIAKSVASDIVKEEIPL
LGISGTTDAAEFTKAKKEFPVIIFGPGNETPHQVNENVSIENYLEMVDVYKRIATEFLS

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; fifth step. [C]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=326, Percent_Identity=26.9938650306748, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1790395, Length=376, Percent_Identity=26.3297872340426, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789236, Length=392, Percent_Identity=22.7040816326531, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17551016, Length=228, Percent_Identity=28.0701754385965, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR010182
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 42012; Mature: 42012

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERQRKIQILKDIVNIDSTNGHEEQVANYLQKLLAEHGIESEKVQYDVDRASLVSEIGSS
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHEEECHHHHHHHHHHCCC
DEKVLAFSGHMDVVDAGDVSKWKFPPFEAAEHEGKIYGRGATDMKSGLAAMIIAMIELHE
CCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
EKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLIIGEPSGHRIVYAHKGSINYT
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCEEEE
VKSTGKNAHSSMPEFGVNAIDNLLLFYNEVEKFVKSIDATNEILGDFIHNVTVIDGGNQV
EECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCEEEEECCCCC
NSIPEKAQLKGNIRSIPEMDNETVKQVLVKIINKLNKQENMNLELIFDYDKQPVFSDKNS
CCCCHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCCCC
DLVHIAKSVASDIVKEEIPLLGISGTTDAAEFTKAKKEFPVIIFGPGNETPHQVNENVSI
HHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCHHCCCCCCH
ENYLEMVDVYKRIATEFLS
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERQRKIQILKDIVNIDSTNGHEEQVANYLQKLLAEHGIESEKVQYDVDRASLVSEIGSS
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHEEECHHHHHHHHHHCCC
DEKVLAFSGHMDVVDAGDVSKWKFPPFEAAEHEGKIYGRGATDMKSGLAAMIIAMIELHE
CCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
EKQKLNGKIRLLATVGEEVGELGAEQLTQKGYADDLDGLIIGEPSGHRIVYAHKGSINYT
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCEEEE
VKSTGKNAHSSMPEFGVNAIDNLLLFYNEVEKFVKSIDATNEILGDFIHNVTVIDGGNQV
EECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCEEEEECCCCC
NSIPEKAQLKGNIRSIPEMDNETVKQVLVKIINKLNKQENMNLELIFDYDKQPVFSDKNS
CCCCHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCCCC
DLVHIAKSVASDIVKEEIPLLGISGTTDAAEFTKAKKEFPVIIFGPGNETPHQVNENVSI
HHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCEEEECCCCCCCHHCCCCCCH
ENYLEMVDVYKRIATEFLS
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]