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The map label for this gene is inlE

Identifier: 226222899

GI number: 226222899

Start: 292840

End: 294339

Strand: Direct

Name: inlE

Synonym: Lm4b_00291

Alternate gene names: 226222899

Gene position: 292840-294339 (Clockwise)

Preceding gene: 226222898

Following gene: 226222900

Centisome position: 10.05

GC content: 36.13

Gene sequence:

>1500_bases
ATGAAAAGAAACAAAGTAATATTAAGAATCGTAGTGACTTTAGCAGCAGTATTGGCAATTACTTTTTGGGTTGGGATGAG
CTCAAAAGAAGTACAAGCAGCGGTAATTGAACAACCAACCCCTATCAATGAAATTTTTACTGATCCAGTGGTTGCTGATA
ATGTGAAAACACTTCTCGAAAAAGCGGATGTAACAGATGAAGTTACGCAAACTGATTTAGATAGTATAACTCAATTATCA
GCAAAATCTGCGGGAATAACAACGATAGAAGGAATGCAGTACTTAACTAATTTATCAGAACTAGAACTAACAGATAACCA
AATAACCGATGTAAGTCCGCTCGCAAATTTAACGAAAATAACAGAGCTTGGATTATCCGGAAATCCATTAAAAGATGTAA
GTGCGCTTGCGGGATTAAAAAGTCTAAAAATGTTGCATCTTATTTATACAGATATTACCGATGTGACTTCCCTTGCAGGT
CTTACCAATTTGCAGGAATTAAATTTGGATATCAATCAAATAACGGATATAAGTCCACTAGCAGCTTTATCTAACTTGCA
AACATTATCACTCGGATATACCCAAGTGAGCGATTTAACCCCAATTGCTAATTTATCTAAACTAACCATATTAAACGCTG
AAAATTGTAAAGTAAGCGATATCTCGCCACTTGCTAGTTTATCTAGTCTTACAGAAGTTTACTTGAGAGAGAATCAAATT
AGCGATGTGAGTCCCCTTGCGAATATTCCCAACTTATCTATTATTGAGTTGACGGATCAAATAATTACCAACCAACCCGT
ATATTATCAAAATAAAATCATTGTTCCAAATGTGATAAAAGGCCTTTCTGGTGAGCTTATTGCACCTGATACTATTAGTG
ACAATGGAACATATACTAGTCCTGATTTAACGTGGGATTTAAACAGCTACATTAATAGTGTTAGTTACACATTTAACCAA
TCAGTCGTTTTCAAAAATGCAACAGTTCCTTTCAGTGGAACAGTCACCCAACCATTAACAGAGGCTTACACTGCCGTTTT
TGATGTGGACGGAGAGCAAACAAGTTTGACAGTAGTTGTGAATGAATTAATTGAAGAACCAACAGTTCCAACCAAGGAAG
GATATACATTCGATGGCTGGTATGACGCAGAAACGAATGGGAATAAATGGGACTTTGCGGTAGATAAAATGCCTGCTAGT
GATATAACTTTATATGCTAAGTTCACTGAGAACGAAGAACCAAATGCTAGTAGTTCAATTAATGTGGAACCAAACGATGA
TAATTCCATCAATGTAGAACCCGACGCTAGCAATTCCATCACTGTAAAACCAACAGAAAATAACGATGGAACAAATAATC
CGAACAGTTCAAGTGATGATAAAGTTAACATCAAGTTACCAATTACTGGGGACGAATGGAATGTGCTTCCCATTTTTGTA
GGAGCAGTTCTTATCGGTATGGGCTTAGTTTTATTCCGCAAAAAACGTCAAACAAAATAA

Upstream 100 bases:

>100_bases
AGTGATTGAATGAAAACCTACTACGCTATAATATAGTAACTCAACTACGAGAACATACAAATTTCTAAAAGCAGAACGAT
CATACAAAGGAGATAGTTTA

Downstream 100 bases:

>100_bases
TTCGATTAATGGCTTGGCTTCTGTGAATTATGCAGGAGCTAAGCCATTTTTTGATTGGAGATTAGGAGGATGAGTTTGCC
TTTTTATGGAACGGGAAAAC

Product: internalin E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKRNKVILRIVVTLAAVLAITFWVGMSSKEVQAAVIEQPTPINEIFTDPVVADNVKTLLEKADVTDEVTQTDLDSITQLS
AKSAGITTIEGMQYLTNLSELELTDNQITDVSPLANLTKITELGLSGNPLKDVSALAGLKSLKMLHLIYTDITDVTSLAG
LTNLQELNLDINQITDISPLAALSNLQTLSLGYTQVSDLTPIANLSKLTILNAENCKVSDISPLASLSSLTEVYLRENQI
SDVSPLANIPNLSIIELTDQIITNQPVYYQNKIIVPNVIKGLSGELIAPDTISDNGTYTSPDLTWDLNSYINSVSYTFNQ
SVVFKNATVPFSGTVTQPLTEAYTAVFDVDGEQTSLTVVVNELIEEPTVPTKEGYTFDGWYDAETNGNKWDFAVDKMPAS
DITLYAKFTENEEPNASSSINVEPNDDNSINVEPDASNSITVKPTENNDGTNNPNSSSDDKVNIKLPITGDEWNVLPIFV
GAVLIGMGLVLFRKKRQTK

Sequences:

>Translated_499_residues
MKRNKVILRIVVTLAAVLAITFWVGMSSKEVQAAVIEQPTPINEIFTDPVVADNVKTLLEKADVTDEVTQTDLDSITQLS
AKSAGITTIEGMQYLTNLSELELTDNQITDVSPLANLTKITELGLSGNPLKDVSALAGLKSLKMLHLIYTDITDVTSLAG
LTNLQELNLDINQITDISPLAALSNLQTLSLGYTQVSDLTPIANLSKLTILNAENCKVSDISPLASLSSLTEVYLRENQI
SDVSPLANIPNLSIIELTDQIITNQPVYYQNKIIVPNVIKGLSGELIAPDTISDNGTYTSPDLTWDLNSYINSVSYTFNQ
SVVFKNATVPFSGTVTQPLTEAYTAVFDVDGEQTSLTVVVNELIEEPTVPTKEGYTFDGWYDAETNGNKWDFAVDKMPAS
DITLYAKFTENEEPNASSSINVEPNDDNSINVEPDASNSITVKPTENNDGTNNPNSSSDDKVNIKLPITGDEWNVLPIFV
GAVLIGMGLVLFRKKRQTK
>Mature_499_residues
MKRNKVILRIVVTLAAVLAITFWVGMSSKEVQAAVIEQPTPINEIFTDPVVADNVKTLLEKADVTDEVTQTDLDSITQLS
AKSAGITTIEGMQYLTNLSELELTDNQITDVSPLANLTKITELGLSGNPLKDVSALAGLKSLKMLHLIYTDITDVTSLAG
LTNLQELNLDINQITDISPLAALSNLQTLSLGYTQVSDLTPIANLSKLTILNAENCKVSDISPLASLSSLTEVYLRENQI
SDVSPLANIPNLSIIELTDQIITNQPVYYQNKIIVPNVIKGLSGELIAPDTISDNGTYTSPDLTWDLNSYINSVSYTFNQ
SVVFKNATVPFSGTVTQPLTEAYTAVFDVDGEQTSLTVVVNELIEEPTVPTKEGYTFDGWYDAETNGNKWDFAVDKMPAS
DITLYAKFTENEEPNASSSINVEPNDDNSINVEPDASNSITVKPTENNDGTNNPNSSSDDKVNIKLPITGDEWNVLPIFV
GAVLIGMGLVLFRKKRQTK

Specific function: Mediates the entry of Listeria monocytogenes into cells [H]

COG id: COG4886

COG function: function code S; Leucine-rich repeat (LRR) protein

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 14 LRR (leucine-rich) repeats [H]

Homologues:

Organism=Homo sapiens, GI4506013, Length=186, Percent_Identity=31.7204301075269, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI55743114, Length=174, Percent_Identity=32.7586206896552, Blast_Score=67, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17531555, Length=216, Percent_Identity=31.9444444444444, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17554124, Length=177, Percent_Identity=29.9435028248588, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358617, Length=185, Percent_Identity=31.3513513513514, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014755
- InterPro:   IPR019948
- InterPro:   IPR014756
- InterPro:   IPR001611
- InterPro:   IPR013378
- InterPro:   IPR019931
- InterPro:   IPR012569
- InterPro:   IPR001899 [H]

Pfam domain/function: PF09479 Flg_new; PF00746 Gram_pos_anchor; PF00560 LRR_1; PF08191 LRR_adjacent [H]

EC number: NA

Molecular weight: Translated: 54244; Mature: 54244

Theoretical pI: Translated: 3.97; Mature: 3.97

Prosite motif: PS50847 GRAM_POS_ANCHORING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNKVILRIVVTLAAVLAITFWVGMSSKEVQAAVIEQPTPINEIFTDPVVADNVKTLLE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHH
KADVTDEVTQTDLDSITQLSAKSAGITTIEGMQYLTNLSELELTDNQITDVSPLANLTKI
HCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHEEECCCCCCCCHHHHHHHHH
TELGLSGNPLKDVSALAGLKSLKMLHLIYTDITDVTSLAGLTNLQELNLDINQITDISPL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCHH
AALSNLQTLSLGYTQVSDLTPIANLSKLTILNAENCKVSDISPLASLSSLTEVYLRENQI
HHHHCCEEEECCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
SDVSPLANIPNLSIIELTDQIITNQPVYYQNKIIVPNVIKGLSGELIAPDTISDNGTYTS
CCCCHHCCCCCCEEEEEHHHHHCCCCEEEECEEECHHHHCCCCCCEECCCCCCCCCCCCC
PDLTWDLNSYINSVSYTFNQSVVFKNATVPFSGTVTQPLTEAYTAVFDVDGEQTSLTVVV
CCCEEEHHHHHHHHEEEECCEEEEECCCCCCCCCCCHHHHHHHEEEEECCCCCCCHHHHH
NELIEEPTVPTKEGYTFDGWYDAETNGNKWDFAVDKMPASDITLYAKFTENEEPNASSSI
HHHHHCCCCCCCCCEEECCEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCEE
NVEPNDDNSINVEPDASNSITVKPTENNDGTNNPNSSSDDKVNIKLPITGDEWNVLPIFV
EECCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHH
GAVLIGMGLVLFRKKRQTK
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRNKVILRIVVTLAAVLAITFWVGMSSKEVQAAVIEQPTPINEIFTDPVVADNVKTLLE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHH
KADVTDEVTQTDLDSITQLSAKSAGITTIEGMQYLTNLSELELTDNQITDVSPLANLTKI
HCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHEEECCCCCCCCHHHHHHHHH
TELGLSGNPLKDVSALAGLKSLKMLHLIYTDITDVTSLAGLTNLQELNLDINQITDISPL
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCHH
AALSNLQTLSLGYTQVSDLTPIANLSKLTILNAENCKVSDISPLASLSSLTEVYLRENQI
HHHHCCEEEECCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCC
SDVSPLANIPNLSIIELTDQIITNQPVYYQNKIIVPNVIKGLSGELIAPDTISDNGTYTS
CCCCHHCCCCCCEEEEEHHHHHCCCCEEEECEEECHHHHCCCCCCEECCCCCCCCCCCCC
PDLTWDLNSYINSVSYTFNQSVVFKNATVPFSGTVTQPLTEAYTAVFDVDGEQTSLTVVV
CCCEEEHHHHHHHHEEEECCEEEEECCCCCCCCCCCHHHHHHHEEEEECCCCCCCHHHHH
NELIEEPTVPTKEGYTFDGWYDAETNGNKWDFAVDKMPASDITLYAKFTENEEPNASSSI
HHHHHCCCCCCCCCEEECCEEECCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCCCEE
NVEPNDDNSINVEPDASNSITVKPTENNDGTNNPNSSSDDKVNIKLPITGDEWNVLPIFV
EECCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHH
GAVLIGMGLVLFRKKRQTK
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9541569 [H]