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The map label for this gene is prpB [C]

Identifier: 226222716

GI number: 226222716

Start: 82925

End: 83698

Strand: Direct

Name: prpB [C]

Synonym: Lm4b_00081

Alternate gene names: 226222716

Gene position: 82925-83698 (Clockwise)

Preceding gene: 226222715

Following gene: 226222717

Centisome position: 2.85

GC content: 45.61

Gene sequence:

>774_bases
ATGACAAAAAACTTTGAAAAACTAAAATCCCTACACGAACTACCCACCCCACTAATCCTCTACAACTGCTGGGACGTCGC
TTCCGCCAGAGCAATCCAACAAGGCGGAGCACCAGTAATAGCGACAAGCAGTTATGCGATTGCTGAGTCACTTGGCGTAG
TGGACGGGGAGCATTTAACTTTTGATGAAATGTTTTCAGTTATTTCGCGCATCGCCGAGAATGTGAGTTTGCCGCTAACG
GTGGATATAGAGACTGGGTATGCTAGAAATTTGGAGGAACTGGCTCAGAATGTGGAGCGGTTGCTTGTGATTGGTGTTTG
TGGTGTGAACTTGGAAGATCAGCTTATTGGCGTTACAAATCCGGGGTTATGCAGTACTGAGGAGCAATGTGATAAAATCG
AGACAATCAAAGAAACGGCGGTGAAAATGAGCACGGAAATTTTTATCAATGCGCGTATAGATGTCTTTTTCCAAGGGCGG
GATGAGACGGCTGACTTGGTGGAAGAAGCGATTAGCCGAGCGAATGCCTATAAAGAAGCCGGCGCGGATGCTATTTTCAT
TCCAGGGCTGTTATCGCCGAATTTAATTCGCGCATTTGTTGAGAAATCACCACTTCCGGTCAATGTCATGAGGATGGATG
GGATGCTCTCAAACGAGGACTTACAAAAAATCGGTGTGAAAAGAATCAGTTATGGACCGTTTAGTTTTTTTCAGGCTAAT
TTAGCGATTCAGCTGGAAGTTGAGCGGATTTTCGAAGGAGGGAAAGCCTTGTGA

Upstream 100 bases:

>100_bases
CAATCCAAAAAATAAAAAAACATCCATGTTAATTTTCCTGGTACCTCCAATCTCCCCACCTGCTATACTAACCCCATCCT
AACAAATGGAGTTGAAACCA

Downstream 100 bases:

>100_bases
TAACGAATCAGCGGGATATTGATAAATATTATGATATGTTAGTGGAGAAAAATTCGAATTATGAAGGCGTATTTTTTGTT
GGAGTGAAAACGACGGGAAT

Product: carboxyphosphonoenolpyruvate phosphonomutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTKNFEKLKSLHELPTPLILYNCWDVASARAIQQGGAPVIATSSYAIAESLGVVDGEHLTFDEMFSVISRIAENVSLPLT
VDIETGYARNLEELAQNVERLLVIGVCGVNLEDQLIGVTNPGLCSTEEQCDKIETIKETAVKMSTEIFINARIDVFFQGR
DETADLVEEAISRANAYKEAGADAIFIPGLLSPNLIRAFVEKSPLPVNVMRMDGMLSNEDLQKIGVKRISYGPFSFFQAN
LAIQLEVERIFEGGKAL

Sequences:

>Translated_257_residues
MTKNFEKLKSLHELPTPLILYNCWDVASARAIQQGGAPVIATSSYAIAESLGVVDGEHLTFDEMFSVISRIAENVSLPLT
VDIETGYARNLEELAQNVERLLVIGVCGVNLEDQLIGVTNPGLCSTEEQCDKIETIKETAVKMSTEIFINARIDVFFQGR
DETADLVEEAISRANAYKEAGADAIFIPGLLSPNLIRAFVEKSPLPVNVMRMDGMLSNEDLQKIGVKRISYGPFSFFQAN
LAIQLEVERIFEGGKAL
>Mature_256_residues
TKNFEKLKSLHELPTPLILYNCWDVASARAIQQGGAPVIATSSYAIAESLGVVDGEHLTFDEMFSVISRIAENVSLPLTV
DIETGYARNLEELAQNVERLLVIGVCGVNLEDQLIGVTNPGLCSTEEQCDKIETIKETAVKMSTEIFINARIDVFFQGRD
ETADLVEEAISRANAYKEAGADAIFIPGLLSPNLIRAFVEKSPLPVNVMRMDGMLSNEDLQKIGVKRISYGPFSFFQANL
AIQLEVERIFEGGKAL

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=234, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 28231; Mature: 28099

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNFEKLKSLHELPTPLILYNCWDVASARAIQQGGAPVIATSSYAIAESLGVVDGEHLT
CCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCC
FDEMFSVISRIAENVSLPLTVDIETGYARNLEELAQNVERLLVIGVCGVNLEDQLIGVTN
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHEEEEEECCCCCCCCEEECCC
PGLCSTEEQCDKIETIKETAVKMSTEIFINARIDVFFQGRDETADLVEEAISRANAYKEA
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GADAIFIPGLLSPNLIRAFVEKSPLPVNVMRMDGMLSNEDLQKIGVKRISYGPFSFFQAN
CCCEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCHHHCCCCCHHHEEEC
LAIQLEVERIFEGGKAL
EEEEEEHHHHHCCCCCC
>Mature Secondary Structure 
TKNFEKLKSLHELPTPLILYNCWDVASARAIQQGGAPVIATSSYAIAESLGVVDGEHLT
CCCHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCC
FDEMFSVISRIAENVSLPLTVDIETGYARNLEELAQNVERLLVIGVCGVNLEDQLIGVTN
HHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHEEEEEECCCCCCCCEEECCC
PGLCSTEEQCDKIETIKETAVKMSTEIFINARIDVFFQGRDETADLVEEAISRANAYKEA
CCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
GADAIFIPGLLSPNLIRAFVEKSPLPVNVMRMDGMLSNEDLQKIGVKRISYGPFSFFQAN
CCCEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCHHHCCCCCHHHEEEC
LAIQLEVERIFEGGKAL
EEEEEEHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]