Click here to switch to the map view.

The map label for this gene is levE [H]

Identifier: 226222662

GI number: 226222662

Start: 27431

End: 27919

Strand: Direct

Name: levE [H]

Synonym: Lm4b_00025

Alternate gene names: 226222662

Gene position: 27431-27919 (Clockwise)

Preceding gene: 226222661

Following gene: 226222663

Centisome position: 0.94

GC content: 39.47

Gene sequence:

>489_bases
ATGGGAAGCAACGGAATTAAACACGTACGCGTAGATGAGAGACTAATTCACGGGCAAGTAGCAACAATGTGGACCAATAC
TATAAAAGCAACGCGAATCATGATTGTTGACGATGCGGTTGTAAAAAATGAAATGGAAAAAGTTGCCTTAAAAACCGCAG
TACCGGCTGGTGTCAAACTAAGCATTCTGACCGTAAAAGGTGCAGCAAATAATATCAATAATGATAAATACGCAGGGCAG
CAAGTTTTCTTAATCGTAAAATCACCTCATGCTCTTCGCGGTTTAGTAGATGCGGGGGTAGAACTTCCCCAAATCAATGT
TGGAAATATGTCGACTAAAGCAGGGAGCCGACAAATCAAAAAATCAGTTAGCGTGACGGATGAAAATTTGGAAGATTTCG
ATTACTTGTCTCAAAAAGGCATTAAAATCACTGCCCAAATGGTTCCGAGTGAAGATGCGGTTGAATTTGCAAGTTTATTA
AAAAAATAA

Upstream 100 bases:

>100_bases
GTTTAGAGGAGGCGATGCTTGGAGCAAAAGAAAATATTCAATTTGTAGAGCAAAAAGCGAGTTATACGATAGTAAGTGAT
GATGGGGAGGAAGAGGACTG

Downstream 100 bases:

>100_bases
GTGATAAGAAGGGAGAACTACAATGGATTTAGCAGTTTGGCAGATTATATTGTTAGTTATACTCGCAGCTTGTACCATTC
TTGATGCTTTAACTTTAGTA

Product: PTS system, fructose-specific IIB component

Products: NA

Alternate protein names: EIIB-Fru; PTS system fructose-specific EIIB component; p18 [H]

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MGSNGIKHVRVDERLIHGQVATMWTNTIKATRIMIVDDAVVKNEMEKVALKTAVPAGVKLSILTVKGAANNINNDKYAGQ
QVFLIVKSPHALRGLVDAGVELPQINVGNMSTKAGSRQIKKSVSVTDENLEDFDYLSQKGIKITAQMVPSEDAVEFASLL
KK

Sequences:

>Translated_162_residues
MGSNGIKHVRVDERLIHGQVATMWTNTIKATRIMIVDDAVVKNEMEKVALKTAVPAGVKLSILTVKGAANNINNDKYAGQ
QVFLIVKSPHALRGLVDAGVELPQINVGNMSTKAGSRQIKKSVSVTDENLEDFDYLSQKGIKITAQMVPSEDAVEFASLL
KK
>Mature_161_residues
GSNGIKHVRVDERLIHGQVATMWTNTIKATRIMIVDDAVVKNEMEKVALKTAVPAGVKLSILTVKGAANNINNDKYAGQQ
VFLIVKSPHALRGLVDAGVELPQINVGNMSTKAGSRQIKKSVSVTDENLEDFDYLSQKGIKITAQMVPSEDAVEFASLLK
K

Specific function: LevD and levE act as negative regulators of the levanase operon. They may be involved in a PTS-mediated phosphorylation of a regulator [H]

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=165, Percent_Identity=35.1515151515151, Blast_Score=109, Evalue=9e-26,
Organism=Escherichia coli, GI87082217, Length=153, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=8e-21,
Organism=Escherichia coli, GI1789527, Length=152, Percent_Identity=36.1842105263158, Blast_Score=92, Evalue=1e-20,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17572; Mature: 17440

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSNGIKHVRVDERLIHGQVATMWTNTIKATRIMIVDDAVVKNEMEKVALKTAVPAGVKL
CCCCCCCEEEHHHHHHCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCEE
SILTVKGAANNINNDKYAGQQVFLIVKSPHALRGLVDAGVELPQINVGNMSTKAGSRQIK
EEEEEECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCCCEECCCCCCCCCHHHHHH
KSVSVTDENLEDFDYLSQKGIKITAQMVPSEDAVEFASLLKK
HHHCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
GSNGIKHVRVDERLIHGQVATMWTNTIKATRIMIVDDAVVKNEMEKVALKTAVPAGVKL
CCCCCCEEEHHHHHHCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCEE
SILTVKGAANNINNDKYAGQQVFLIVKSPHALRGLVDAGVELPQINVGNMSTKAGSRQIK
EEEEEECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCCCEECCCCCCCCCHHHHHH
KSVSVTDENLEDFDYLSQKGIKITAQMVPSEDAVEFASLLKK
HHHCCCCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117666; 9141695; 9384377; 9551099 [H]