Click here to switch to the map view.

The map label for this gene is manX [H]

Identifier: 225861981

GI number: 225861981

Start: 2031190

End: 2031660

Strand: Reverse

Name: manX [H]

Synonym: SPT_2176

Alternate gene names: 225861981

Gene position: 2031660-2031190 (Counterclockwise)

Preceding gene: 225861982

Following gene: 225861980

Centisome position: 96.19

GC content: 32.27

Gene sequence:

>471_bases
ATGTCTATAGAGTTTGTTCGTATTGATGACCGTCTGGTACATGGTCAAGTTGTCACTACGTGGCTAAAAAAGTATGATAT
TGAGCAAGTTATCATTGTTAATGATCGCATCTCAGAAGATAAAACACGACAATCTATTTTAAAGATTTCTGCACCGGTAG
GTTTAAAAATTGTTTTCTTTAGTGTAAAACGGTTTGTGGAAGTTTTAAACTCTGTGCCAATAAAAAAGAGAACAATGCTG
ATATATACAAATCCAAAAGATGTGTATGATTCTATTGAAGGAAATTTAAAATTGGAGTACCTCAATGTAGGACAGATGAG
TAAAACGGAGGAAAATGAAAAGGTAACGGGAGGTGTAGCTCTAGGTGAAGAAGACAAATATTATTTTAAGAAAATAGTTG
ATAAGGGAACGAGAGTTGAAATTCAAATGGTTCCTAATGATAAAGTTACAATGTTGGAAAAATTTTTATAA

Upstream 100 bases:

>100_bases
GTTATGATTCAAAAATCAATTTAGAAGAAATTGTTCAAAATGCTCAAAATAGTTTGTTTAATGTTAAACAACAACTTAAC
GTAGAGGAGGAAGAGGATTT

Downstream 100 bases:

>100_bases
AAATAATTTAAGGAGGTACAGTATATGCTATTCACACAAGCATTACTGGTGACATTAGTTGGGATTATTGCCACTATTGA
CTATAATGGACCGTTATTTA

Product: PTS system, IIB component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQSILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTML
IYTNPKDVYDSIEGNLKLEYLNVGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL

Sequences:

>Translated_156_residues
MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQSILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTML
IYTNPKDVYDSIEGNLKLEYLNVGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL
>Mature_155_residues
SIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQSILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLI
YTNPKDVYDSIEGNLKLEYLNVGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=155, Percent_Identity=35.4838709677419, Blast_Score=103, Evalue=4e-24,
Organism=Escherichia coli, GI87082217, Length=155, Percent_Identity=23.8709677419355, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI1789527, Length=148, Percent_Identity=31.0810810810811, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 18047; Mature: 17916

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQSILKISAPVGLKIVFF
CCEEEEEECCEEECHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHEECCCCCEEHHHH
SVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLNVGQMSKTEENEKVTGGVA
HHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCEEEEEEECCCCCCCCCCCEEECCEE
LGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL
ECCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
SIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQSILKISAPVGLKIVFF
CEEEEEECCEEECHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHEECCCCCEEHHHH
SVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLNVGQMSKTEENEKVTGGVA
HHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCEEEEEEECCCCCCCCCCCEEECCEE
LGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL
ECCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]