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The map label for this gene is aspS [H]

Identifier: 225861929

GI number: 225861929

Start: 1977714

End: 1979477

Strand: Reverse

Name: aspS [H]

Synonym: SPT_2122

Alternate gene names: 225861929

Gene position: 1979477-1977714 (Counterclockwise)

Preceding gene: 225861930

Following gene: 225861928

Centisome position: 93.72

GC content: 45.75

Gene sequence:

>1764_bases
ATGAAACGTAGTATGTATGCTGGTCGTGTTCGTGAGGAACACATCGGACAAGAAATAACCTTGAAAGGATGGGTTGGCCG
TCGTCGTGACCTTGGTGGTTTGATCTTTATCGATCTTCGTGACCGTGAAGGAATCATGCAGTTGGTTATCAACCCTGAAA
AAGTATCTGCAGAGGTTATGGCAACAGCTGAAAGCCTTCGTAGCGAATTTGTTATTGAGGTGACTGGTCAGGTCGCTGCG
CGTGAGCAAGCCAATGATAAGTTGCCAACTGGTGCGGTTGAGTTAAACGTGACAGCTCTTATTGTGCTTAACACAGCTAA
GACAACACCATTTGAGATTAAGGATGGCATTGAGGCAAATGACGATACACGTTTGCGTTACCGTTACCTTGACCTTCGTC
GTCCAGAAATGTTGGAAAATCTTAAACTTCGTGCCAAGGTGACCCACTCTATCCGCAACTACTTGGATGAGTTGGAGTTT
ATCGACGTGGAGACACCATTCCTTTCTAAGTCAACGCCTGAAGGGGCGCGTGATTATTTAGTGCCGTCTCGTGTTAATAA
GGGGCATTTTTACGCTCTTCCTCAAAGTCCACAAATCACGAAACAGCTCTTGATGAATGCTGGTTTTGACCGTTACTACC
AAATCGTTAAATGTTTCCGTGACGAAGACTTGCGTGGAGACCGCCAGCCTGAATTTACTCAGGTCGACTTGGAAACGTCC
TTCCTTACTGAGCAAGAAATCCAAGATATTACAGAAGGCTTGATCGCGCGCGTGATGAAGGAAACAAAAGGCATCGAAGT
AACGCTACCGTTCCCTCGTGTGAAATACGATGATGCTATGGCTCTTTACGGTTCTGACAAGCCAGATACTCGTTTTGACA
TGTTGCTTCAGGACTTGACAGAAGTGGTCAAAGGTGTAGACTTTAAAGTCTTTTCAGAAGCACCTGCTGTAAAAGCGATT
GTGGTCAAAGGAGCTGCGGACAACTATTCACGTAAAGACATCGACAAGATGACGGAAGTAGCCAAACAGTATGGTGCCAA
AGGTCTTGCTTGGGTTAAGGTGGTTGATGGAGAATTAAACGGACCAGTTGCCAAGTTCTTGACTGGTATCCAAGAAGAAT
TGACAACAGCGCTTGCTCTTGAAGATAAGGACTTGGTTCTCTTTGTGGCGGATACGCTTGAAGTGGCTAATGCAACACTG
GGTGCCCTTCGTGGACGTATTGCTAAAGAGCTTGGCTTGATTGATAATGATAAGTTCAACTTCCTTTGGGTGGTTGACTG
GCCGATGTTTGAATGGTCTGAAGAAGAAGGCCGCTACATGAGCGCCCACCATCCTTTCACCCTTCCACAGGAAGAGACTG
CTCACGAATTAGAAGGTGATTTGGCTAAGGTTCGTGCCATTGCTTACGATATCGTCTTGAACGGTTATGAGCTTGGTGGT
GGTAGCCTTCGTATCAACCAAAAAGACCTTCAAGAACGCATGTTCAAGGCTCTTGGTTTCTCAGCTGAAGAAGCCAATGA
CCAGTTTGGTTTCCTTCTTGAAGCCATGGACTATGGTTTCCCACCACACGGTGGTTTGGCTATCGGGCTTGACCGTTTTG
TCATGTTGCTTGCTGGAGAAGAAAATATCCGTGAAGTCATTGCCTTTCCTAAGAACAACAAGGCAACTGACCCAATGACA
CAAGCTCCATCAACAGTCGCTCTCAAACAACTAGAGGAACTCAGCTTACAAGTAGAAGAAGATGAAACAAGCAAAACGAA
TTAA

Upstream 100 bases:

>100_bases
TCATCTGACTTTTAAAGACTGGCAAACTTTTCTGATGATTTTCATGAAGAGCCTGCGCTTTTATGGTAAAATAGTAACAG
AATAAAAGAGGAGAGAAATA

Downstream 100 bases:

>100_bases
GCGGTGGCGCTATTATCTGCGCCGCTTTGCTTATCAGATAAAAATTTTACGTGTCTTACAAAGTATCTCTCGAGAAAAGT
ATGATGAGAAGATTTCGGCC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAA
REQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLTEQEIQDITEGLIARVMKETKGIEVTLPFPRVKYDDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAI
VVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATL
GALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMT
QAPSTVALKQLEELSLQVEEDETSKTN

Sequences:

>Translated_587_residues
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAA
REQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLTEQEIQDITEGLIARVMKETKGIEVTLPFPRVKYDDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAI
VVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATL
GALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMT
QAPSTVALKQLEELSLQVEEDETSKTN
>Mature_587_residues
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAA
REQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
FLTEQEIQDITEGLIARVMKETKGIEVTLPFPRVKYDDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAI
VVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATL
GALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMT
QAPSTVALKQLEELSLQVEEDETSKTN

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=40.9015025041736, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI45439306, Length=296, Percent_Identity=26.0135135135135, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI194272210, Length=255, Percent_Identity=28.2352941176471, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI5031815, Length=255, Percent_Identity=28.2352941176471, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI4758762, Length=286, Percent_Identity=25.8741258741259, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788173, Length=592, Percent_Identity=50.6756756756757, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1789256, Length=295, Percent_Identity=26.1016949152542, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790571, Length=291, Percent_Identity=25.085910652921, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI32566633, Length=600, Percent_Identity=35.5, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=309, Percent_Identity=23.3009708737864, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=309, Percent_Identity=23.3009708737864, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=311, Percent_Identity=23.1511254019293, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=300, Percent_Identity=25, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=302, Percent_Identity=25.8278145695364, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=628, Percent_Identity=31.5286624203822, Blast_Score=259, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6323011, Length=243, Percent_Identity=29.2181069958848, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=253, Percent_Identity=26.8774703557312, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=37.0860927152318, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=28.4518828451883, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24640849, Length=255, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640851, Length=255, Percent_Identity=26.6666666666667, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66150; Mature: 66150

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVM
CCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHH
ATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEAN
HHHHHHHCCEEEEECCCHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCC
DDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEFIDVETPFLSKSTPEGARDYL
CCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCHHCC
VPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLTEQEIQDITEGLIARVMKETKGIEVTLPFPRVKYDDAMALYGSDKPDTRFDMLLQDLT
HCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
EVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELN
HHHHCCCEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
GPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATLGALRGRIAKELGLIDNDKFN
CCHHHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
FLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
EEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECEEECC
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGE
CEEEECHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHCCCCCCCCEEECHHHHEEEECCC
ENIREVIAFPKNNKATDPMTQAPSTVALKQLEELSLQVEEDETSKTN
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCC
>Mature Secondary Structure
MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREGIMQLVINPEKVSAEVM
CCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHH
ATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIVLNTAKTTPFEIKDGIEAN
HHHHHHHCCEEEEECCCHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCC
DDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEFIDVETPFLSKSTPEGARDYL
CCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCHHCC
VPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKCFRDEDLRGDRQPEFTQVDLETS
CCCCCCCCEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHH
FLTEQEIQDITEGLIARVMKETKGIEVTLPFPRVKYDDAMALYGSDKPDTRFDMLLQDLT
HCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHH
EVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDIDKMTEVAKQYGAKGLAWVKVVDGELN
HHHHCCCEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
GPVAKFLTGIQEELTTALALEDKDLVLFVADTLEVANATLGALRGRIAKELGLIDNDKFN
CCHHHHHHHHHHHHHHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
FLWVVDWPMFEWSEEEGRYMSAHHPFTLPQEETAHELEGDLAKVRAIAYDIVLNGYELGG
EEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECEEECC
GSLRINQKDLQERMFKALGFSAEEANDQFGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGE
CEEEECHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHCCCCCCCCEEECHHHHEEEECCC
ENIREVIAFPKNNKATDPMTQAPSTVALKQLEELSLQVEEDETSKTN
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA