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The map label for this gene is ackA [H]

Identifier: 225861863

GI number: 225861863

Start: 1900315

End: 1901505

Strand: Reverse

Name: ackA [H]

Synonym: SPT_2039

Alternate gene names: 225861863

Gene position: 1901505-1900315 (Counterclockwise)

Preceding gene: 225861864

Following gene: 225861862

Centisome position: 90.03

GC content: 40.47

Gene sequence:

>1191_bases
ATGACAAAAACAATTGCAATCAATGCAGGAAGTTCAAGTTTGAAATGGCAATTATACTTAATGCCAGAAGAAAAAGTATT
GGCGAAAGGTTTGATTGAACGTATCGGTTTGAAAGATTCAATTTCAACTGTAAAATTTGACGGCCGTTCTGAACAACAAA
TTTTGGATATTGAAAATCATACACAAGCCGTTAAAATTTTATTGGATGACTTGATTCGTTTCGATATTATCAAGGCTTAT
GACGAGATTACAGGTGTTGGACATCGTGTTGTTGCTGGTGGAGAATATTTCAAAGAATCAACAGTTGTTGAGGGAGATGT
TTTAGAAAAAGTTGAAGAGTTGAGTTTGTTGGCTCCTCTACACAACCCGGCCAATGCAGCAGGTGTTCGTGCCTTCAAGG
AATTGTTGCCAGACATTACCAGTGTAGTTGTTTTTGATACTTCCTTCCACACAAGTATGCCAGAGAAAGCTTATCGCTAC
CCTCTACCAACAAAATATTACACAGAAAACAAGGTTCGTAAATACGGTGCTCATGGTACAAGTCACCAGTTTGTAGCAGG
AGAAGCTGCAAAACTCTTGGGACGTCCATTAGAAGACTTGAAGTTAATTACCTGTCATATTGGTAACGGAGGCTCAATTA
CAGCTGTGAAAGCCGGCAAATCTGTAGACACTTCTATGGGGTTCACTCCTCTTGGTGGTATTATGATGGGAACGCGTACA
GGGGATATTGATCCAGCTATCATTCCTTATTTAATGCAATATACAGAGGATTTTAACACACCAGAAGATATCAGTCGTGT
TCTTAACCGTGAATCAGGTCTTTTGGGAGTTTCTGCTAATTCTAGCGATATGCGCGATATAGAAGCAGCTGTAGCAGAAG
GGAATCACGAGGCTAGCTTGGCTTATGAAATGTATGTTGACCGTATCCAAAAACATATCGGTCAGTACCTTGCAGTGCTA
AATGGAGCAGATGCCATTGTTTTCACAGCAGGTGTCGGTGAAAATGCAGAGAGTTTCCGTCGTGATGTAATCTCAGGGAT
TTCGTGGTTTGGTTGTGATGTTGATGATGAAAAGAATGTCTTTGGCGTTACAGGAGACATCTCAACAGAGGCAGCTAAAA
TCCGTGTCTTGGTTATTCCAACAGATGAAGAATTAGTCATTGCCCGTGACGTTGAACGCTTGAAAAAATAA

Upstream 100 bases:

>100_bases
TGAAATTTAAAGAAAATTTTCAAAAATGGACTCAAGGTACTGAAATATAAAATAGATTTTGTTATAATAGTTGAAAACGC
TTAAAAAGGGGTATCATGTT

Downstream 100 bases:

>100_bases
GTGAAACTAAAAAAATATTCAATACAAGGAGTTGGGAAAGTTATTTTTCCAGCTTCTTTTTCTGATGAAATTGTCCAAAA
CCTTGCTATGATTGGCTTTT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAY
DEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRT
GDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVL
NGADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK

Sequences:

>Translated_396_residues
MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAY
DEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRT
GDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVL
NGADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK
>Mature_395_residues
TKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYD
EITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRYP
LPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRTG
DIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLN
GADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=41.3098236775819, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI145693184, Length=395, Percent_Identity=40.7594936708861, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43331; Mature: 43200

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENH
CCEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECHHH
TQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCEECHHHHHHHHHHHHHHCC
HNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRYPLPTKYYTENKVRKYGAHGT
CCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHCCCCC
SHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRT
CCCEECCHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
GDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASL
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHH
AYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCCE
FGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK
EEEECCCCCCCEEEEEEEEECCCCEEHHHHHHHHCC
>Mature Secondary Structure 
TKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTVKFDGRSEQQILDIENH
CEEEEEECCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECHHH
TQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTVVEGDVLEKVEELSLLAPL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCEECHHHHHHHHHHHHHHCC
HNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRYPLPTKYYTENKVRKYGAHGT
CCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHCCCCC
SHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKAGKSVDTSMGFTPLGGIMMGTRT
CCCEECCHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCC
GDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLGVSANSSDMRDIEAAVAEGNHEASL
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHH
AYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGENAESFRRDVISGISWFGCDVDDEKNV
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCEECCCCCCCCCE
FGVTGDISTEAAKIRVLVIPTDEELVIARDVERLKK
EEEECCCCCCCEEEEEEEEECCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA