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The map label for this gene is wcaF [H]

Identifier: 225686233

GI number: 225686233

Start: 391082

End: 391696

Strand: Reverse

Name: wcaF [H]

Synonym: BMEA_B0402

Alternate gene names: 225686233

Gene position: 391696-391082 (Counterclockwise)

Preceding gene: 225686234

Following gene: 225686232

Centisome position: 33.04

GC content: 60.81

Gene sequence:

>615_bases
ATGACGATCACGCAAGCCGGTTCTCCACACTCCATTGCCGGCAGAAAACAAACCTCCCGCCCGCTTGAAGGCGGCGCAAC
CTTTACGCTGAAGCATCGCCTGCAGCGGCTTTTGTGGCAGGCTGCGTGGCTTGCCCTTGCAGCGTGGACCCCTTCATGCC
TGTGGCGATGGCGCGGCATGGTGCTAAACGCCTTCGGAGCCGATATCCACAAGAGCGCAATCGTTCGCGCAAGCGCACAT
ATCTGGTGGCCGGGCAATCTGTCCATGGGCGCACATTCCTCGCTTGGCCCCGGCGCGATCTGCTACAATGTTGCGCCGGT
AACGCTCGCCCCCTTCGCCATTGTCTCGCAGCGCGCGCATCTTTGCACGGCAGGCCACGATATCGATCAGGCGAGCTTTC
CCCTCACTGCCGCACCGATTCACATCGCTGCCTATGGCTGGGTGGCGGCAGAGGCTTTTGTCGGCCCTGGCGTACATATT
GGCGAGGGTGCAGTGCTGGGTGCGCGCAGCGTCAGCTTCCGCGATCTGGATGCATGGGCCGTTTACACAGGCAATCCAGC
AAAGCCCGTACGCCAGCGGCGCAAAAACACAGATTATTCAATCCACCGGTTTTAA

Upstream 100 bases:

>100_bases
ACATGGCCCTTTTCCTCATCCCCATCCTGTCGCTTTGTGTCATTCGCAATCGATCCGTCTATCTGCCGATGTCGCCGCAG
CTTTCCTGAAGGTATTTGCC

Downstream 100 bases:

>100_bases
AGAATAAAACACATGCCCTCCTGGCGAAGCAGCACTGCGTCTGCCCATTGAAAAAATATGATATGAAGCGCAACAATAAG
TAGTAGAAAAATCCGGAGGC

Product: chloramphenicol acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTITQAGSPHSIAGRKQTSRPLEGGATFTLKHRLQRLLWQAAWLALAAWTPSCLWRWRGMVLNAFGADIHKSAIVRASAH
IWWPGNLSMGAHSSLGPGAICYNVAPVTLAPFAIVSQRAHLCTAGHDIDQASFPLTAAPIHIAAYGWVAAEAFVGPGVHI
GEGAVLGARSVSFRDLDAWAVYTGNPAKPVRQRRKNTDYSIHRF

Sequences:

>Translated_204_residues
MTITQAGSPHSIAGRKQTSRPLEGGATFTLKHRLQRLLWQAAWLALAAWTPSCLWRWRGMVLNAFGADIHKSAIVRASAH
IWWPGNLSMGAHSSLGPGAICYNVAPVTLAPFAIVSQRAHLCTAGHDIDQASFPLTAAPIHIAAYGWVAAEAFVGPGVHI
GEGAVLGARSVSFRDLDAWAVYTGNPAKPVRQRRKNTDYSIHRF
>Mature_203_residues
TITQAGSPHSIAGRKQTSRPLEGGATFTLKHRLQRLLWQAAWLALAAWTPSCLWRWRGMVLNAFGADIHKSAIVRASAHI
WWPGNLSMGAHSSLGPGAICYNVAPVTLAPFAIVSQRAHLCTAGHDIDQASFPLTAAPIHIAAYGWVAAEAFVGPGVHIG
EGAVLGARSVSFRDLDAWAVYTGNPAKPVRQRRKNTDYSIHRF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=156, Percent_Identity=39.7435897435897, Blast_Score=127, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 22051; Mature: 21920

Theoretical pI: Translated: 10.78; Mature: 10.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITQAGSPHSIAGRKQTSRPLEGGATFTLKHRLQRLLWQAAWLALAAWTPSCLWRWRGM
CCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VLNAFGADIHKSAIVRASAHIWWPGNLSMGAHSSLGPGAICYNVAPVTLAPFAIVSQRAH
HHHHHCCHHHHHHHEEECEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCHH
LCTAGHDIDQASFPLTAAPIHIAAYGWVAAEAFVGPGVHIGEGAVLGARSVSFRDLDAWA
EEECCCCCCCCCCCCEECCEEEEEHHHHHHHHHCCCCCEECCCEEEECCCCCEECCCEEE
VYTGNPAKPVRQRRKNTDYSIHRF
EEECCCCHHHHHHHCCCCCEEECC
>Mature Secondary Structure 
TITQAGSPHSIAGRKQTSRPLEGGATFTLKHRLQRLLWQAAWLALAAWTPSCLWRWRGM
CCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VLNAFGADIHKSAIVRASAHIWWPGNLSMGAHSSLGPGAICYNVAPVTLAPFAIVSQRAH
HHHHHCCHHHHHHHEEECEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCHH
LCTAGHDIDQASFPLTAAPIHIAAYGWVAAEAFVGPGVHIGEGAVLGARSVSFRDLDAWA
EEECCCCCCCCCCCCEECCEEEEEHHHHHHHHHCCCCCEECCCEEEECCCCCEECCCEEE
VYTGNPAKPVRQRRKNTDYSIHRF
EEECCCCHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]