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The map label for this gene is mcbR [H]

Identifier: 225686204

GI number: 225686204

Start: 351832

End: 352509

Strand: Reverse

Name: mcbR [H]

Synonym: BMEA_B0370

Alternate gene names: 225686204

Gene position: 352509-351832 (Counterclockwise)

Preceding gene: 225686214

Following gene: 225686201

Centisome position: 29.73

GC content: 54.72

Gene sequence:

>678_bases
ATGACCGAAGCATCTGGAAATGAATGGATAAAGCCTATCCAACGGCAGAACTTGAGCGAACTCGCTTACACAACTCTGCG
TGAAGCCTTGATGGAGGGTCGGCTGAAACCAGGTGAAGACCTGCCGCTTCGTCCCATTTCCCGCAGTTTCGGAATCAGCG
CGACGCCCATGCGCGAGGCACTGTTGCGGCTGGTTGCAGAACATGCGCTGGAAATCGACGCGCGCGGCCGAATTTCAGTT
CCACATCTCACACGCGCCGAGCTTCTGGAAGTGAGGAAATTGCGTGGCTTTCTGGAAGGAAATGCCGCGCGTGAAGCCAC
TCTCAATGCCACTCCAGAGGCAGTGGACGCACTCGAACAAATCCACTTTGAACTGATCACTGCACAGGACGCAGGTGATT
TTCCTACGGCCATCGGCTTCAATACCAAGTTTCATCTGAAGATGTGCGAAATTGCCGGGCTGCCCGTAACGCACGAGTTG
GTTTCAAATCTTTGGGTTCGCTGCGGACCGCTGATCTCACATCTTTACGACGCAGGCATTCCAGCCAACTGGAACCCCCA
CCCCCACGTACTCATCGTCAAGGCCATCCGCGCTAGAGATCCCGATATGGCATATAAAGAACTGAACCGCGACATTGAAG
GGAGCGGCGCCGGGCTTCTGGCTCATGTGAAAGATTGA

Upstream 100 bases:

>100_bases
CCTCAACCGCTAGCGTGCCATCACATTTTCCGTTATAAGACACATGTTATAACAACAAGTCAATTTGAATCCGCAGGCGG
CGTCGACAGGAACGAATAAA

Downstream 100 bases:

>100_bases
ACCAGCCTTTACGAGCGAACCGAAACCCTCATGAAAGGGGCGTGCCAGCACGCGGGGTCGAGGCCGCGCGTCAGGAAATA
CGCGCGGGCAATCTCGAAAA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTEASGNEWIKPIQRQNLSELAYTTLREALMEGRLKPGEDLPLRPISRSFGISATPMREALLRLVAEHALEIDARGRISV
PHLTRAELLEVRKLRGFLEGNAAREATLNATPEAVDALEQIHFELITAQDAGDFPTAIGFNTKFHLKMCEIAGLPVTHEL
VSNLWVRCGPLISHLYDAGIPANWNPHPHVLIVKAIRARDPDMAYKELNRDIEGSGAGLLAHVKD

Sequences:

>Translated_225_residues
MTEASGNEWIKPIQRQNLSELAYTTLREALMEGRLKPGEDLPLRPISRSFGISATPMREALLRLVAEHALEIDARGRISV
PHLTRAELLEVRKLRGFLEGNAAREATLNATPEAVDALEQIHFELITAQDAGDFPTAIGFNTKFHLKMCEIAGLPVTHEL
VSNLWVRCGPLISHLYDAGIPANWNPHPHVLIVKAIRARDPDMAYKELNRDIEGSGAGLLAHVKD
>Mature_224_residues
TEASGNEWIKPIQRQNLSELAYTTLREALMEGRLKPGEDLPLRPISRSFGISATPMREALLRLVAEHALEIDARGRISVP
HLTRAELLEVRKLRGFLEGNAAREATLNATPEAVDALEQIHFELITAQDAGDFPTAIGFNTKFHLKMCEIAGLPVTHELV
SNLWVRCGPLISHLYDAGIPANWNPHPHVLIVKAIRARDPDMAYKELNRDIEGSGAGLLAHVKD

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=179, Percent_Identity=34.6368715083799, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24929; Mature: 24798

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEASGNEWIKPIQRQNLSELAYTTLREALMEGRLKPGEDLPLRPISRSFGISATPMREA
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHH
LLRLVAEHALEIDARGRISVPHLTRAELLEVRKLRGFLEGNAAREATLNATPEAVDALEQ
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHH
IHFELITAQDAGDFPTAIGFNTKFHLKMCEIAGLPVTHELVSNLWVRCGPLISHLYDAGI
HHHHHEECCCCCCCCCEECCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PANWNPHPHVLIVKAIRARDPDMAYKELNRDIEGSGAGLLAHVKD
CCCCCCCCCEEEEEEHHCCCCCHHHHHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
TEASGNEWIKPIQRQNLSELAYTTLREALMEGRLKPGEDLPLRPISRSFGISATPMREA
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHH
LLRLVAEHALEIDARGRISVPHLTRAELLEVRKLRGFLEGNAAREATLNATPEAVDALEQ
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHH
IHFELITAQDAGDFPTAIGFNTKFHLKMCEIAGLPVTHELVSNLWVRCGPLISHLYDAGI
HHHHHEECCCCCCCCCEECCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PANWNPHPHVLIVKAIRARDPDMAYKELNRDIEGSGAGLLAHVKD
CCCCCCCCCEEEEEEHHCCCCCHHHHHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]