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The map label for this gene is bepF [H]

Identifier: 225686174

GI number: 225686174

Start: 314909

End: 316132

Strand: Reverse

Name: bepF [H]

Synonym: BMEA_B0335

Alternate gene names: 225686174

Gene position: 316132-314909 (Counterclockwise)

Preceding gene: 225686175

Following gene: 225686173

Centisome position: 26.67

GC content: 59.64

Gene sequence:

>1224_bases
ATGGTTGCCTTTTGGACGTGCCGAAACGCTTGGTTCCAACACCTTCCTTTTGCGAAGCGCGGCGACGAAAATGCGCCATC
TGGACCACGCCGGCTGCGCCCCTGGTTCCTCGTTCTGGCTCTGGGGCTTGCAGCGTGCAGCGAAGACAAGTCAGCTCCGC
AGCAAGCCGCTCCCCTGCCCCCGATACCTGTCGGCGTCATCAAGATAACAGAGCGCCCCACCCATCCCCAGCTCTCCTTC
GTCGGACGTGTCGAGGCAACCGATAGCGTCGATCTGATAGCCCGCGTCGATGGCTTTCTCGACAAGCGCACCTTCACCGA
AGGTCAGGCCGTAAAGACCGGGGATTTGCTGTTTGTCCTGCAAAAGGATGCGCTTCAGGCGGCGCAGGCCAATCTTGCAA
AAGCGCAGGCCGATGCCGACAACCTGAAACTCCAGACCGAGCGCGCCCGCTCGCTCTACAAGCAGAAGACCGTTTCGCAA
GCCATGCTTGATGATCGTGTGGCAGCGGAAAAGCAGGCTTTGGCCGTCGTGCAGCAGGCGCAGGCTTCGCTGGAACGGGC
GCAAATCAATCTCGGCTATACCGATATACGCGCGCCTTTTTCCGGACGCATCGGCATGGCCAATTTCAGCGTCGGCGCCC
TTGTCGGGCCGTCCTCAGGCCCTCTGGCAACGATCGTCAGCCAGGACCCGATTTACGTCACCTTCCCCGTCAGCGACAAG
ACCATTCTCGATTTAACCGAAGGCGGGAGAACGGCCACAGACCGCAGCAATGTAGCCGTCAGCCTGAAGCTCTCCAACGG
CATGACATATCCGCAAACGGGGGCCATCGACTTTACCGGGATCAAGATTAATCCCAATACCGATACGCTGATGGTCCGGG
CACAGTTTCCCAATCCGAACAATGTCCTGATCGACGGGCAGTATGTTCAGGTAACGGCGGCGAGCAAACACCCCGTTGAA
GCCCTGCTCGTCCCGCAAAAGGCAATCATGACCGACCAGAGCGGCAATTACGTTCTGGCCGTCGGTGAAGATAACAAGGT
CATCCAGCGCCAGATCACGCAAGGTTCGACCTTTGGCAGCAATGTTGTGGTCAAGTCCGGCCTCGCGGTGGGCGATCAGG
TCGTGGTCGATGGCCTCCAGCGCATCCGGCCCGGCCAGAAGGTCGATCCGCAGATCGTCGATGCAACCACGCCCGCCCAG
AAAGCCATGTCGGTGGGGAATTGA

Upstream 100 bases:

>100_bases
AACCGGCAGCCCTGCAAAAATCGCACGGCGCTTAGCTGGGCCTTAGCGAAATATAACCCGGCCTGTTGACGAACGGGTCG
ATATGCATGGAGTGCTGACG

Downstream 100 bases:

>100_bases
TCCATGCTGTCATCCGTCTTCATTAACAGGCCGCGCCTTGCCATTGTGATCGCTATCGTCATCACGCTGGCAGGGCTGAT
CGCGGTCACACGCATTCCCG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLPPIPVGVIKITERPTHPQLSF
VGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFVLQKDALQAAQANLAKAQADADNLKLQTERARSLYKQKTVSQ
AMLDDRVAAEKQALAVVQQAQASLERAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFPVSDK
TILDLTEGGRTATDRSNVAVSLKLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYVQVTAASKHPVE
ALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGSTFGSNVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDATTPAQ
KAMSVGN

Sequences:

>Translated_407_residues
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLPPIPVGVIKITERPTHPQLSF
VGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFVLQKDALQAAQANLAKAQADADNLKLQTERARSLYKQKTVSQ
AMLDDRVAAEKQALAVVQQAQASLERAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFPVSDK
TILDLTEGGRTATDRSNVAVSLKLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYVQVTAASKHPVE
ALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGSTFGSNVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDATTPAQ
KAMSVGN
>Mature_407_residues
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLPPIPVGVIKITERPTHPQLSF
VGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFVLQKDALQAAQANLAKAQADADNLKLQTERARSLYKQKTVSQ
AMLDDRVAAEKQALAVVQQAQASLERAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFPVSDK
TILDLTEGGRTATDRSNVAVSLKLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPNNVLIDGQYVQVTAASKHPVE
ALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGSTFGSNVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDATTPAQ
KAMSVGN

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=367, Percent_Identity=34.6049046321526, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1786668, Length=386, Percent_Identity=34.7150259067358, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789665, Length=366, Percent_Identity=32.7868852459016, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI87082036, Length=415, Percent_Identity=30.6024096385542, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87081951, Length=172, Percent_Identity=31.3953488372093, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43680; Mature: 43680

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLP
CEEEEEHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCC
PIPVGVIKITERPTHPQLSFVGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFVL
CCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEECCCEEEEE
QKDALQAAQANLAKAQADADNLKLQTERARSLYKQKTVSQAMLDDRVAAEKQALAVVQQA
EHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QASLERAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFPVSDK
HHHHHHHEEECCCCEECCCCCCCEEEEECCEEEEECCCCCCEEEEECCCCEEEEEECCCC
TILDLTEGGRTATDRSNVAVSLKLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPN
EEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCC
NVLIDGQYVQVTAASKHPVEALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGSTFGS
EEEECCEEEEEEECCCCCCEEEECCCHHHEECCCCCEEEEECCCCHHHHHHHHCCCCCCC
NVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDATTPAQKAMSVGN
CEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHCCCC
>Mature Secondary Structure
MVAFWTCRNAWFQHLPFAKRGDENAPSGPRRLRPWFLVLALGLAACSEDKSAPQQAAPLP
CEEEEEHHHHHHHHCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCC
PIPVGVIKITERPTHPQLSFVGRVEATDSVDLIARVDGFLDKRTFTEGQAVKTGDLLFVL
CCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEECCCEEEEE
QKDALQAAQANLAKAQADADNLKLQTERARSLYKQKTVSQAMLDDRVAAEKQALAVVQQA
EHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QASLERAQINLGYTDIRAPFSGRIGMANFSVGALVGPSSGPLATIVSQDPIYVTFPVSDK
HHHHHHHEEECCCCEECCCCCCCEEEEECCEEEEECCCCCCEEEEECCCCEEEEEECCCC
TILDLTEGGRTATDRSNVAVSLKLSNGMTYPQTGAIDFTGIKINPNTDTLMVRAQFPNPN
EEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCC
NVLIDGQYVQVTAASKHPVEALLVPQKAIMTDQSGNYVLAVGEDNKVIQRQITQGSTFGS
EEEECCEEEEEEECCCCCCEEEECCCHHHEECCCCCEEEEECCCCHHHHHHHHCCCCCCC
NVVVKSGLAVGDQVVVDGLQRIRPGQKVDPQIVDATTPAQKAMSVGN
CEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]