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The map label for this gene is fadH [C]

Identifier: 225686076

GI number: 225686076

Start: 212747

End: 214021

Strand: Reverse

Name: fadH [C]

Synonym: BMEA_B0225

Alternate gene names: 225686076

Gene position: 214021-212747 (Counterclockwise)

Preceding gene: 225686078

Following gene: 225686073

Centisome position: 18.05

GC content: 62.98

Gene sequence:

>1275_bases
ATGTCGCTTTTTTCTCCGCTGGTGCTGAACAATGGCGCCACCATACCAAACCGTATTGCCAAGGCCGCCATGGAGGAGAA
GATGGCCGATGCCGACCACGCCCCTTCCGCCGGTCTTTTGCGGCTGTACAAGGCGTGGGCCGAAGGAGAGACCGGGCTTA
TCATCACCGGCAATGTGATGGTTGATGCCCGCGCCATGACAGGCCCCGGCGGGGTTGTGCTGGAAGACGACCGCCATCTC
GACCGTTTCCAGGCATGGGCTGAAGCGGGCCGGACGCGGGGGGCGCATATCTGGATGCAGATCAACCATCCGGGCCGGCA
GATGCCGGCCGCGCTTGGGCAGGAAACGCTGGCCCCTTCCGCAATTGCCCTTGATCTCGGCGCGCAATCGAAGCGGTTTC
CGATACCGCGCGAAATGAGCGAAGCCGATATTGCCGATGTGGAGCGCCGCTTCATCAACACGGCGCAGCTTGCAGAACGC
GCGGACTTTACCGGCGTCGAAATCCATGCGGCGCACGGCTATCTTCTAAGCCAGTTCCTCTCGCCGCTTTCAAACCGGCG
ACAGGACCGCTGGGGCGGCAGCCTCGAAAACCGCGCGCGCCTGCTTGTCAATATCGTGCGCGGCATTCGCGCGGCGGTTT
CGCCGGGCTTTTGCGTTGCCGTGAAGCTTAATTCGGCTGATTTCCAGCGCGGCGGCTTTTTGCCGGAAGATGCCCGCGCC
GTGGTCATGATGCTGGCGCCGCTCGGTGTCGATCTGGTGGAGCTTTCCGGCGGCAGCTACGAGGCGCCCGCCATGATGGG
ATCCGCCCGCGATGAGCGGACATTGGCGCGCGAGGCCTATTTTCTCGATTTCGCCCGCGATATTGCGGAAATTGCCACGA
TGCCGCTGATGGTCACCGGGGGTATCCGCCGCCGTGAGGTGGCAGAACAGGTTATCGAAAGCGGAATAGCCATGGCAGGC
ATTGCCACCGCACTTGCAATCGAACCCAACCTGCCGCGCAACTGGCGGCTGGGGCGCGCCGATGCGCCGAAACTGAAGCC
CATTGCGTGGAAGAACAAGCCGCTTGCCGCTTCGGCCCATATGGCCGCCGTCAAATATCAATTGACACGGTTGAGCTGCC
AACGGCGCACCGCGCCCGGCATTTCGCCGGTCTGGGCGCTTGCCGTTTCACAGATCGATGCGTTTTTCCGCGCCCGCCGT
TACCGGCAATGGATGGTGGATCGGCGGGCGACACCCGGCAGGTATCGCCCGGACCAGTTCATTCGCCAGAGTTGA

Upstream 100 bases:

>100_bases
CTCGCCGATTTTCATATCGACCCACCTTTCAGAAATTGCTTGACCTTAAACTTAACTTAAACGTTAAGCTCTGTCCAAAT
CTATTTCTGGAGTATTCTCA

Downstream 100 bases:

>100_bases
ACTGCTCGATAATGCCGCGCGACTTGAGGTCGTTCAGCCTATCATCGTCCAGTTTCGCCAATTGCTGGAGAAGCGTGTCG
GTATGTTCACCCAGCATGGG

Product: NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSLFSPLVLNNGATIPNRIAKAAMEEKMADADHAPSAGLLRLYKAWAEGETGLIITGNVMVDARAMTGPGGVVLEDDRHL
DRFQAWAEAGRTRGAHIWMQINHPGRQMPAALGQETLAPSAIALDLGAQSKRFPIPREMSEADIADVERRFINTAQLAER
ADFTGVEIHAAHGYLLSQFLSPLSNRRQDRWGGSLENRARLLVNIVRGIRAAVSPGFCVAVKLNSADFQRGGFLPEDARA
VVMMLAPLGVDLVELSGGSYEAPAMMGSARDERTLAREAYFLDFARDIAEIATMPLMVTGGIRRREVAEQVIESGIAMAG
IATALAIEPNLPRNWRLGRADAPKLKPIAWKNKPLAASAHMAAVKYQLTRLSCQRRTAPGISPVWALAVSQIDAFFRARR
YRQWMVDRRATPGRYRPDQFIRQS

Sequences:

>Translated_424_residues
MSLFSPLVLNNGATIPNRIAKAAMEEKMADADHAPSAGLLRLYKAWAEGETGLIITGNVMVDARAMTGPGGVVLEDDRHL
DRFQAWAEAGRTRGAHIWMQINHPGRQMPAALGQETLAPSAIALDLGAQSKRFPIPREMSEADIADVERRFINTAQLAER
ADFTGVEIHAAHGYLLSQFLSPLSNRRQDRWGGSLENRARLLVNIVRGIRAAVSPGFCVAVKLNSADFQRGGFLPEDARA
VVMMLAPLGVDLVELSGGSYEAPAMMGSARDERTLAREAYFLDFARDIAEIATMPLMVTGGIRRREVAEQVIESGIAMAG
IATALAIEPNLPRNWRLGRADAPKLKPIAWKNKPLAASAHMAAVKYQLTRLSCQRRTAPGISPVWALAVSQIDAFFRARR
YRQWMVDRRATPGRYRPDQFIRQS
>Mature_423_residues
SLFSPLVLNNGATIPNRIAKAAMEEKMADADHAPSAGLLRLYKAWAEGETGLIITGNVMVDARAMTGPGGVVLEDDRHLD
RFQAWAEAGRTRGAHIWMQINHPGRQMPAALGQETLAPSAIALDLGAQSKRFPIPREMSEADIADVERRFINTAQLAERA
DFTGVEIHAAHGYLLSQFLSPLSNRRQDRWGGSLENRARLLVNIVRGIRAAVSPGFCVAVKLNSADFQRGGFLPEDARAV
VMMLAPLGVDLVELSGGSYEAPAMMGSARDERTLAREAYFLDFARDIAEIATMPLMVTGGIRRREVAEQVIESGIAMAGI
ATALAIEPNLPRNWRLGRADAPKLKPIAWKNKPLAASAHMAAVKYQLTRLSCQRRTAPGISPVWALAVSQIDAFFRARRY
RQWMVDRRATPGRYRPDQFIRQS

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=343, Percent_Identity=28.2798833819242, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1787939, Length=217, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=348, Percent_Identity=36.4942528735632, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17564188, Length=349, Percent_Identity=34.9570200573066, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI72001454, Length=346, Percent_Identity=34.6820809248555, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17559804, Length=350, Percent_Identity=34, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17540738, Length=352, Percent_Identity=34.0909090909091, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17565138, Length=343, Percent_Identity=33.2361516034985, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17566914, Length=368, Percent_Identity=35.054347826087, Blast_Score=171, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17566916, Length=374, Percent_Identity=29.6791443850267, Blast_Score=131, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6325086, Length=81, Percent_Identity=39.5061728395062, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=77, Percent_Identity=41.5584415584416, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 46599; Mature: 46468

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFSPLVLNNGATIPNRIAKAAMEEKMADADHAPSAGLLRLYKAWAEGETGLIITGNVM
CCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCEE
VDARAMTGPGGVVLEDDRHLDRFQAWAEAGRTRGAHIWMQINHPGRQMPAALGQETLAPS
EEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCHHHCCCC
AIALDLGAQSKRFPIPREMSEADIADVERRFINTAQLAERADFTGVEIHAAHGYLLSQFL
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHH
SPLSNRRQDRWGGSLENRARLLVNIVRGIRAAVSPGFCVAVKLNSADFQRGGFLPEDARA
HHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHH
VVMMLAPLGVDLVELSGGSYEAPAMMGSARDERTLAREAYFLDFARDIAEIATMPLMVTG
HHHHHHHCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
GIRRREVAEQVIESGIAMAGIATALAIEPNLPRNWRLGRADAPKLKPIAWKNKPLAASAH
CCHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHH
MAAVKYQLTRLSCQRRTAPGISPVWALAVSQIDAFFRARRYRQWMVDRRATPGRYRPDQF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IRQS
HCCC
>Mature Secondary Structure 
SLFSPLVLNNGATIPNRIAKAAMEEKMADADHAPSAGLLRLYKAWAEGETGLIITGNVM
CCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCEE
VDARAMTGPGGVVLEDDRHLDRFQAWAEAGRTRGAHIWMQINHPGRQMPAALGQETLAPS
EEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHCHHHCCCC
AIALDLGAQSKRFPIPREMSEADIADVERRFINTAQLAERADFTGVEIHAAHGYLLSQFL
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHH
SPLSNRRQDRWGGSLENRARLLVNIVRGIRAAVSPGFCVAVKLNSADFQRGGFLPEDARA
HHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHH
VVMMLAPLGVDLVELSGGSYEAPAMMGSARDERTLAREAYFLDFARDIAEIATMPLMVTG
HHHHHHHCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
GIRRREVAEQVIESGIAMAGIATALAIEPNLPRNWRLGRADAPKLKPIAWKNKPLAASAH
CCHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHH
MAAVKYQLTRLSCQRRTAPGISPVWALAVSQIDAFFRARRYRQWMVDRRATPGRYRPDQF
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IRQS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]