Click here to switch to the map view.

The map label for this gene is lutR [H]

Identifier: 225685891

GI number: 225685891

Start: 19834

End: 20574

Strand: Reverse

Name: lutR [H]

Synonym: BMEA_B0025

Alternate gene names: 225685891

Gene position: 20574-19834 (Counterclockwise)

Preceding gene: 225685903

Following gene: 225685888

Centisome position: 1.74

GC content: 58.7

Gene sequence:

>741_bases
ATGAAGGAAATAGCCACCCGCGCGGCGGGGCGCGCGACCGGCCATTTGCCTGAAGAAATTGCTCGACAGATTGAAAAACG
CATTCTCGACGGCGAGTTTTCCGTCGGCTCCAAATTGCCGAGCGAGCGGGAGCTTTCCGTGGAATACGGTGTCAGCCGCC
CGGTGGTGCGCGAGGCGCTTTCCCAGCTCAAGCTGGACGGCCTTGTGGAAGCGCGCGCGGGCAGCGGCGTTTTCGTGATC
GACGAAAGCAACCAGAAAGCCTTCCGAATTCAGCCGCTTGTCGTGGAAGAAGCGCAATCGCTCGAACAGCTCATGGAACT
GCTGGTGGCCATTGAGGTTGCCGCCACGCGCATTGCTGCCGTCAACCGTACGCCTGAAGACCTGAAGCAGATCAAACGCG
CGCTGATCGGGCTGGAATATGCCATCATCAGCGACAAGCTGGGCGACGAGGAGGATTATGCCTTCCACGATGCCATCGTG
ATCGCCACGCATAATCCGCACTTCATCGCCTTGTGCAAACATCTCGAATATGGCGCGCGCCATGTGATCCGGCAGGCGCG
CACCAATACGCGGTCCAACCTGAAAGACATGCTGGACGCCGTTCAGGACGAGCACAAGGCGATCTATGAAGCGATAGAAC
TGGGCGACCCGGAACAGGCTGGAATTGCAGCCGAGCGCCATTTGCGCAATGCCACGCAGCGCATGAAAGTCTATCTTTCA
GCCGATAACCGGACCAACTGA

Upstream 100 bases:

>100_bases
TTGACATTAACGGTGGAGTCTGGTTGACTTGTTTGACCAGTTAAACCATCTTCAAGCGAAGGATCGGTTTCCAGTGGAAT
TGAAGTCGAGGAGGAATGAC

Downstream 100 bases:

>100_bases
TAGCAAAACCCGTCAGGCCGGTTGATCCGCGCTTCTTCCCCGCGATTACCAGCGCAGGCAGAAGCGCCCGCCAAGCGCAC
CCAGCACGCCTGTTCCCACA

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKEIATRAAGRATGHLPEEIARQIEKRILDGEFSVGSKLPSERELSVEYGVSRPVVREALSQLKLDGLVEARAGSGVFVI
DESNQKAFRIQPLVVEEAQSLEQLMELLVAIEVAATRIAAVNRTPEDLKQIKRALIGLEYAIISDKLGDEEDYAFHDAIV
IATHNPHFIALCKHLEYGARHVIRQARTNTRSNLKDMLDAVQDEHKAIYEAIELGDPEQAGIAAERHLRNATQRMKVYLS
ADNRTN

Sequences:

>Translated_246_residues
MKEIATRAAGRATGHLPEEIARQIEKRILDGEFSVGSKLPSERELSVEYGVSRPVVREALSQLKLDGLVEARAGSGVFVI
DESNQKAFRIQPLVVEEAQSLEQLMELLVAIEVAATRIAAVNRTPEDLKQIKRALIGLEYAIISDKLGDEEDYAFHDAIV
IATHNPHFIALCKHLEYGARHVIRQARTNTRSNLKDMLDAVQDEHKAIYEAIELGDPEQAGIAAERHLRNATQRMKVYLS
ADNRTN
>Mature_246_residues
MKEIATRAAGRATGHLPEEIARQIEKRILDGEFSVGSKLPSERELSVEYGVSRPVVREALSQLKLDGLVEARAGSGVFVI
DESNQKAFRIQPLVVEEAQSLEQLMELLVAIEVAATRIAAVNRTPEDLKQIKRALIGLEYAIISDKLGDEEDYAFHDAIV
IATHNPHFIALCKHLEYGARHVIRQARTNTRSNLKDMLDAVQDEHKAIYEAIELGDPEQAGIAAERHLRNATQRMKVYLS
ADNRTN

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786303, Length=218, Percent_Identity=34.8623853211009, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790780, Length=237, Percent_Identity=31.6455696202532, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790032, Length=233, Percent_Identity=28.3261802575107, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994955, Length=216, Percent_Identity=28.7037037037037, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI87082208, Length=227, Percent_Identity=28.6343612334802, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789621, Length=240, Percent_Identity=27.9166666666667, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789353, Length=231, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI48994961, Length=223, Percent_Identity=28.6995515695067, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27427; Mature: 27427

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIATRAAGRATGHLPEEIARQIEKRILDGEFSVGSKLPSERELSVEYGVSRPVVREAL
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHH
SQLKLDGLVEARAGSGVFVIDESNQKAFRIQPLVVEEAQSLEQLMELLVAIEVAATRIAA
HHHHHHHHHEECCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRTPEDLKQIKRALIGLEYAIISDKLGDEEDYAFHDAIVIATHNPHFIALCKHLEYGAR
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHH
HVIRQARTNTRSNLKDMLDAVQDEHKAIYEAIELGDPEQAGIAAERHLRNATQRMKVYLS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEE
ADNRTN
CCCCCC
>Mature Secondary Structure
MKEIATRAAGRATGHLPEEIARQIEKRILDGEFSVGSKLPSERELSVEYGVSRPVVREAL
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCHHHHHHH
SQLKLDGLVEARAGSGVFVIDESNQKAFRIQPLVVEEAQSLEQLMELLVAIEVAATRIAA
HHHHHHHHHEECCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNRTPEDLKQIKRALIGLEYAIISDKLGDEEDYAFHDAIVIATHNPHFIALCKHLEYGAR
HCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHH
HVIRQARTNTRSNLKDMLDAVQDEHKAIYEAIELGDPEQAGIAAERHLRNATQRMKVYLS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEE
ADNRTN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]