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The map label for this gene is pbpF [H]

Identifier: 225852112

GI number: 225852112

Start: 595596

End: 597752

Strand: Reverse

Name: pbpF [H]

Synonym: BMEA_A0622

Alternate gene names: 225852112

Gene position: 597752-595596 (Counterclockwise)

Preceding gene: 225852140

Following gene: 225852111

Centisome position: 28.12

GC content: 59.25

Gene sequence:

>2157_bases
ATGCGTAATACGGCAGAAAAAGATCGCGGAAACGACAAGCGTAAGCCCTTCCGGGTTTCGCGCCTGATCGGACTCGACGC
CTGGATTGATTCCGCGCTCTACAATTTACATTTCCGCTTGGGCGAATGGTGGGAAAACATCACGATTTTCTCCCGGCGTT
TTCGCGTGCGCGGCTTTCGCCGTTTCACGGTGGAAGTGCTGGACGAAGGCTTTACGCTCGGCGTCGCCGGTTCCGTGCTG
ATGTTGATGCTGGCGCTGCCAGCCTTTGAGGAAACCAAGAAGGACTGGCGCGCGCAGGACGATTATGCCGTCACCTTTCT
TGACCGTTATGGCAACGAGATTGGCCGGCGCGGTATTCTGCACCGGCAGGCGGTGCCTATCGATGAATTGCCGGATCATG
TCATCAAGGCTGTGCTCGGCACCGAGGACCGTCGCTTCTTCGACCATTACGGCATTGATCTCATGGGGCTTAGCCGAGCG
TTAAGCCAGAACATGCGCGCCAACGGCGTGGTTCAGGGCGGCTCGACCATCACGCAGCAGCTTGCCAAAAACCTGTTTCT
TTCCAATGAGCGCACCATCGAACGCAAGGTCAAGGAAGCCTTTCTCGCGCTCTGGCTTGAAAGCAATCTCAGCAAGAAGG
AAATCCTCCAGCTTTACCTCGACCGTGCCTATATGGGCGGCGGCACTTTCGGCATTGCAGCCGCGTCCGAGTTTTATTTC
GGCAAGAATGTAAAGGACATCTCGCTGGCCGAGGCAGCCATGCTGGCCGGCCTGTTCAAGGCTCCGGCGAAATTCGCGCC
GCACGTCAACCTGCCTGCAGCTCGCGCCCGCGCCAATGTCGTTCTCTCCAACATGGTGGAAAGCGGCTTTCTCAGCGAAG
GTCAGGTTGCCGTCGCGCGCCGCCACCCGGCCAGCGTCATAGATCGCGCCAAGGATGAAAGCCCCGACTATTTCCTGGAC
TGGGCCTTCGACGAGGTGAAAAAGGTTGCAGACAGGTTCAACCAGCATACGCTGATCGTGCGCACCACGCTTGACCGCAA
TATCCAGAAAGCCGCCGAAGAATCGCTTGAATTTCACCTTCGCCAATATGGCAAGGAATATAACGTATCGGAGGCGGCCA
CCGTCGTGCTTGCCAATGACGGTTCCGTGCGCGCGCTCGTTGGCGGGCGCGACTATGGCGAAAGCCAGTTCAACCGCGCA
ACCAGGGCGCTGCGGCAGGCCGGTTCATCCTTCAAGCCCTATGTCTATGCGGCTGCCATGGAAAAGGGGCTGACACCAAG
CACCATCGTTTCCGATGCACCGATCAGCTGGGGCAACTGGTCGCCGCGCAATTACGGGCGCAGTTTTGCTGGCCGGGTTG
ATCTCACCACCGCGCTCGTCCGCTCGCTCAACAGCGTGCCGGTGCGCCTTGCCCGCGATTATCTGACCACGGCGCCCGTT
GTCGCGCTCACAAAGGCAATGGGTGTGGAATCGCACATCTCCTCCCATAAGACGATGGTGCTCGGCACATCCGAAATGAC
CGTCATGGATCAGGCAACAGGCTTTAACGTCTTCGCCAATGCGGGCATGGCCGGAAACCGTCACGCTTTCACGCAAATCC
TGGCATCCGACGGAAAGGTTCTTTGGGATTTTGGCCGTGACGCACCAAAGCCGCACCGCGCCCTTTCGGAAAAGGCCGCA
CTTGAGATGAACTCCATGCTGGTGCAGGTGCCCGAGCGCGGCACGGGCCGGCGCGCCGCACTTACGATGACGCGCGTGGC
AGGCAAAACGGGTACGACCCAGAACTATCGCGATGCCTGGTTCGTGGGCTTTACCGGAAATTTCACCGCCGCCGTCTGGT
TCGGCAACGATAATTTCACGCCCATGAAGGAACTGACCGGCGGTGTTCTGCCAGCCATGGCCTGGCAGCGCATGATGGCA
TACGCCCATCAGAATATCGAATTGAAGCCGCTGCCGGGCGTCAACCCGCCCTTCCCCGCCCAGCCGAAGAACCCGCCCGC
ACAGGTGGCCGATACGAGGCCGCACGAAACAATGGCTGCCCCACCACGGGTTTTATCGCCGCTGGCGACAAAAATTCTCA
AGGAACTGCATGACCGCTTCCTTGCCGCGCCGCCTCTGCCTACGATAGCCGAGCGCACAAAAGTTTCGGTACTCTGA

Upstream 100 bases:

>100_bases
TTTCATTTAGCTATTTTGCGTGAGCTAGCTTATTTATCTGTTATGATAATGAGGCAAATCTCGCCTTGTCTCCGACAACC
CTATGCCGAAGTCAGTGTTC

Downstream 100 bases:

>100_bases
GGGGCGATGTTCAAAACGATTTTCAAGGTAGTGGCTGTGCTTGCGCTCGCGCTTGGCGGGGGCATCTGGAGCGTGGATTA
CGCACTGGACAGTTTTGAAG

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MRNTAEKDRGNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFRRFTVEVLDEGFTLGVAGSVL
MLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGILHRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRA
LSQNMRANGVVQGGSTITQQLAKNLFLSNERTIERKVKEAFLALWLESNLSKKEILQLYLDRAYMGGGTFGIAAASEFYF
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVARRHPASVIDRAKDESPDYFLD
WAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHLRQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRA
TRALRQAGSSFKPYVYAAAMEKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
VALTKAMGVESHISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKVLWDFGRDAPKPHRALSEKAA
LEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAWFVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMA
YAHQNIELKPLPGVNPPFPAQPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL

Sequences:

>Translated_718_residues
MRNTAEKDRGNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFRRFTVEVLDEGFTLGVAGSVL
MLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGILHRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRA
LSQNMRANGVVQGGSTITQQLAKNLFLSNERTIERKVKEAFLALWLESNLSKKEILQLYLDRAYMGGGTFGIAAASEFYF
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVARRHPASVIDRAKDESPDYFLD
WAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHLRQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRA
TRALRQAGSSFKPYVYAAAMEKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
VALTKAMGVESHISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKVLWDFGRDAPKPHRALSEKAA
LEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAWFVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMA
YAHQNIELKPLPGVNPPFPAQPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL
>Mature_718_residues
MRNTAEKDRGNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFRRFTVEVLDEGFTLGVAGSVL
MLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGILHRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRA
LSQNMRANGVVQGGSTITQQLAKNLFLSNERTIERKVKEAFLALWLESNLSKKEILQLYLDRAYMGGGTFGIAAASEFYF
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVARRHPASVIDRAKDESPDYFLD
WAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHLRQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRA
TRALRQAGSSFKPYVYAAAMEKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
VALTKAMGVESHISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKVLWDFGRDAPKPHRALSEKAA
LEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAWFVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMA
YAHQNIELKPLPGVNPPFPAQPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=541, Percent_Identity=34.0110905730129, Blast_Score=245, Evalue=6e-66,
Organism=Escherichia coli, GI1788867, Length=519, Percent_Identity=31.2138728323699, Blast_Score=199, Evalue=7e-52,
Organism=Escherichia coli, GI87082258, Length=288, Percent_Identity=36.8055555555556, Blast_Score=182, Evalue=8e-47,
Organism=Escherichia coli, GI1789601, Length=183, Percent_Identity=36.6120218579235, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79757; Mature: 79757

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTAEKDRGNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFTVEVLDEGFTLGVAGSVLMLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGIL
HHHHHHHHCCCEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCC
HRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRALSQNMRANGVVQGGSTITQQ
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCHHHHHH
LAKNLFLSNERTIERKVKEAFLALWLESNLSKKEILQLYLDRAYMGGGTFGIAAASEFYF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVAR
CCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEC
RHPASVIDRAKDESPDYFLDWAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHL
CCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHH
RQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRATRALRQAGSSFKPYVYAAAM
HHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHH
EKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
HCCCCCHHEECCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VALTKAMGVESHISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKV
HHHHHHHCHHHHHCCCCEEEEECCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCE
LWDFGRDAPKPHRALSEKAALEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAW
EEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHEEHHHHCCCCCCCCCCCCEE
FVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMAYAHQNIELKPLPGVNPPFPA
EEEEECCEEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
QPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MRNTAEKDRGNDKRKPFRVSRLIGLDAWIDSALYNLHFRLGEWWENITIFSRRFRVRGFR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFTVEVLDEGFTLGVAGSVLMLMLALPAFEETKKDWRAQDDYAVTFLDRYGNEIGRRGIL
HHHHHHHHCCCEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCC
HRQAVPIDELPDHVIKAVLGTEDRRFFDHYGIDLMGLSRALSQNMRANGVVQGGSTITQQ
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCEECCCHHHHHH
LAKNLFLSNERTIERKVKEAFLALWLESNLSKKEILQLYLDRAYMGGGTFGIAAASEFYF
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GKNVKDISLAEAAMLAGLFKAPAKFAPHVNLPAARARANVVLSNMVESGFLSEGQVAVAR
CCCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEC
RHPASVIDRAKDESPDYFLDWAFDEVKKVADRFNQHTLIVRTTLDRNIQKAAEESLEFHL
CCCHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHH
RQYGKEYNVSEAATVVLANDGSVRALVGGRDYGESQFNRATRALRQAGSSFKPYVYAAAM
HHHCCCCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHH
EKGLTPSTIVSDAPISWGNWSPRNYGRSFAGRVDLTTALVRSLNSVPVRLARDYLTTAPV
HCCCCCHHEECCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VALTKAMGVESHISSHKTMVLGTSEMTVMDQATGFNVFANAGMAGNRHAFTQILASDGKV
HHHHHHHCHHHHHCCCCEEEEECCCEEEEECCCCCEEEECCCCCCCHHHHHHHHHCCCCE
LWDFGRDAPKPHRALSEKAALEMNSMLVQVPERGTGRRAALTMTRVAGKTGTTQNYRDAW
EEECCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHEEHHHHCCCCCCCCCCCCEE
FVGFTGNFTAAVWFGNDNFTPMKELTGGVLPAMAWQRMMAYAHQNIELKPLPGVNPPFPA
EEEEECCEEEEEEECCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
QPKNPPAQVADTRPHETMAAPPRVLSPLATKILKELHDRFLAAPPLPTIAERTKVSVL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]