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The map label for this gene is ispE [H]

Identifier: 225851931

GI number: 225851931

Start: 419300

End: 420199

Strand: Reverse

Name: ispE [H]

Synonym: BMEA_A0426

Alternate gene names: 225851931

Gene position: 420199-419300 (Counterclockwise)

Preceding gene: 225851932

Following gene: 225851930

Centisome position: 19.77

GC content: 60.22

Gene sequence:

>900_bases
ATGACAACATTCCAGAACGCCGGACCATCGATAACGCGCCTTGCGCCTGCAAAGATCAATCTGGCGCTGCATGTGACCGG
GCGACGTGACGACGGCTATCATCTTCTCGACATGCTGGTGGTCTTTGCCGATCAGGGCGATCGCATCCACATCGAAAAAG
CCGGCAGCGACAGTTTTACGGTCAGCGGCCCTTTTGCAAGCGGCATCCCCGCCGGGCGGGGCAATCTGGTGCTGAAAGCG
CGCGATGCGCTGCGCCAGCATGGCGGGCCAGACCTTTCACCTGTTGCCATTCATCTGGAAAAGAACCTGCCTATTGCTTC
CGGCATTGGCGGCGGATCGAGCGATGCGGCAGCAACCCTGCTTGCGCTCAACACGCTCTGGCAACTCGATCTCGATTTTG
AAATGCTGGCCGCTATCGGCCTTTCCCTCGGTGCAGACCTTCCCATGTGCCTGCATGGCGCAGCCCATGGCACACCGCTG
ATCGCGCGCGGCATTGGTGAAGAGCTGAACGATGTTTCCGGAATTGCGGCCCTTCCCATGCTGCTCGTCAATGACGGTAC
CGCGCTTGCCACGCCCGACGTGTTCCGCGCGCTCACCCGCCGCGAGAACGCCCCCCTGCCGCCTCCGGCCTGTGAAGGGA
CAGACGCCCTTTGCGCCTATCTGCGTGAAACACGCAATGATCTTCTGCCCGCAGCCATCAGCCTTGCACCGCAGATCGAA
CCCAAACTCGCCCTGCTTCGCGCCAAGGGTGCACTTTATGCGCGAATGTCCGGCTCCGGCGCAACATGCTTTGCCATTTT
TAGCGATGAATCAGCCCTGACACGCGCGGCAAAAGAGATTGCGGATGAAAATCCGGGCTGGTTTGCAGTTCCTTCGCACA
GCTTTCCTTCCAGAGCCTGA

Upstream 100 bases:

>100_bases
CACAGAACCGTGCCCATGGCACGCTGGTGCAAGATCCCAAGACCGGCGAATATTACGTCCGCAAGGATTGAGCCCCCGGC
TGTAGTGACGAATTACCCCG

Downstream 100 bases:

>100_bases
TCTTTCTCCCATCGTCTTCGTTTTAAGTACGGATCGTTACGCCGCGCCAGTTTTTGCGCGAATTTTCGCGCGAAATGGTA
TTGCAAAATAGCCCTATTGT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTTFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADQGDRIHIEKAGSDSFTVSGPFASGIPAGRGNLVLKA
RDALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATLLALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPL
IARGIGEELNDVSGIAALPMLLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIE
PKLALLRAKGALYARMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA

Sequences:

>Translated_299_residues
MTTFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADQGDRIHIEKAGSDSFTVSGPFASGIPAGRGNLVLKA
RDALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATLLALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPL
IARGIGEELNDVSGIAALPMLLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIE
PKLALLRAKGALYARMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA
>Mature_298_residues
TTFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADQGDRIHIEKAGSDSFTVSGPFASGIPAGRGNLVLKAR
DALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATLLALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPLI
ARGIGEELNDVSGIAALPMLLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIEP
KLALLRAKGALYARMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=283, Percent_Identity=32.8621908127208, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 31238; Mature: 31107

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADQGDRIHIEKAGSDSFT
CCCCCCCCCCEEEECCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEE
VSGPFASGIPAGRGNLVLKARDALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATL
EECCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCCHHHHHH
LALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPLIARGIGEELNDVSGIAALPM
HHHHHHHEECCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHCE
LLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIE
EEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PKLALLRAKGALYARMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA
HHHEEEEECCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
TTFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADQGDRIHIEKAGSDSFT
CCCCCCCCCEEEECCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEE
VSGPFASGIPAGRGNLVLKARDALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATL
EECCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCCHHHHHH
LALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPLIARGIGEELNDVSGIAALPM
HHHHHHHEECCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHCE
LLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIE
EEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PKLALLRAKGALYARMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA
HHHEEEEECCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA