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The map label for this gene is gatB [H]

Identifier: 225629961

GI number: 225629961

Start: 108426

End: 109847

Strand: Reverse

Name: gatB [H]

Synonym: WRi_001070

Alternate gene names: 225629961

Gene position: 109847-108426 (Counterclockwise)

Preceding gene: 225629962

Following gene: 225629960

Centisome position: 7.6

GC content: 37.41

Gene sequence:

>1422_bases
ATGACGAAAGAGGATTGGGAAGCGGTAATTGGGCTTGAGGTACACGCTCAAGTTTCTTCTAATACGAAGCTATTTTCTAG
CTCATCAACGGAATTTGGTGCTGAGCATAACACTCAAGTTTCTCTAGTTGATGCGGCAATGCCAGGTACGCTGCCAATAC
TAAATTATTACTGCATAGAGCAAGCAATACGCACTGGTCTTGCACTTTCTGCAGAAATTAATAAGTATTCTTACTTTGAT
CGGAAAAATTATTTTTATCCTGATTTACCGCAAGGTTACCAGATAACTCAGTTTTTTGAGCCAATAGTTAAAAACGGTAG
AGTATTTATCAACGACAATGAAAAAGAAATAAGAATTGCGAGAATTCATTTAGAGCAAGATGCAGGAAAGAGCGTTCATG
AGGAAAGCAAAACTTATGTAGATTTAAATCGTGCAGGGGTTGCTTTAATCGAAATTGTTTCAGAACCAGATCTCCGTTCA
TCTGCGGAAGCTGCAGAATGCATGAAAAAATTGAGGCAGATTTTGCGTTACACTGGTTCGTGTGATGGTGATATGGAAAA
GGGATCACTTCGTTGTGATGCAAATGTTTCTGTCCGCCTAAAAGGCAGTAATACATTTGGCACTCGTTGTGAGATAAAAA
ATCTGAACTCGATACGTCATATTGTGCAAGCTATAGACTATGAAATACAAAGACAAATTGAAATTTTAGAAAGTGGAGAA
GAAATAAGTCAAGATACCTTATTGTTTGACGTTGCTTCGGGAAAAACAAAAGTGATGAGAAGCAAAGAGGATGCAAGCGA
TTATAGATACTTCCCTGAGCCTGATTTATTACCTGTTGAGGTAAGCCAGGAGAAAATTGATTTAATCCAATCATCTTTGC
CCGAGTTGCCAGATCAAAAAAAGCTCCGATACATTGAGGAATTAGGTATCAATGAATACGATGCAAACGTCATTACTTCC
GATAAGGCGATTGCTGATTACTTTGAGGAATTGATAAAAAAACATGATTCAAAGCTCGCGGTCACCTGGTTAACTGTAGA
GCTTTTCGGTCGTTTAAATAAAGCAAATATTGATATTGTGAGCTCCCCAATCAAAGCAAATGCTTTGTCAGAACTCTTAG
ATTTTATCGTTGATGGAACAATCTCTGCTAAACTTGGTAAACAAGTTTTTGATATTATGTTTGAAACTGGAAAGCCTGCA
TCCCTTATTATAGAAGAGCAGGATCTCAAGCAAATAACTGACAAATGTCAAATTTCAGAGGTAATAGATAAAATCATCAA
TAGCAACCAAGATAAAGTTCAAGAATACAAAAGCGGTAAAACAAGATTATACGGATTCTTTGTCGGCGAAGTCATGAAAC
TCACCAAGGGAAAAGCCAGCCCTGATGTCGTGAATTCAATTTTGAGTGAGAGATTAGGATAA

Upstream 100 bases:

>100_bases
TGGGTAAAACAATCTGCATACCACTTAGCTAACATGAGTTTTGTTTTGACTTTTTGTGCTAATTTTGCAGAATATTAAGA
TTATCATTTAAATAAAATAC

Downstream 100 bases:

>100_bases
AAACAGGGGTAATGTATCTAGAAGATGATTCTACTTGGGGGGTCAAACGCATTTACCATGGTATTACCTTTCAAGAGTTA
TTGATCGTAATATCTCTAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTKEDWEAVIGLEVHAQVSSNTKLFSSSSTEFGAEHNTQVSLVDAAMPGTLPILNYYCIEQAIRTGLALSAEINKYSYFD
RKNYFYPDLPQGYQITQFFEPIVKNGRVFINDNEKEIRIARIHLEQDAGKSVHEESKTYVDLNRAGVALIEIVSEPDLRS
SAEAAECMKKLRQILRYTGSCDGDMEKGSLRCDANVSVRLKGSNTFGTRCEIKNLNSIRHIVQAIDYEIQRQIEILESGE
EISQDTLLFDVASGKTKVMRSKEDASDYRYFPEPDLLPVEVSQEKIDLIQSSLPELPDQKKLRYIEELGINEYDANVITS
DKAIADYFEELIKKHDSKLAVTWLTVELFGRLNKANIDIVSSPIKANALSELLDFIVDGTISAKLGKQVFDIMFETGKPA
SLIIEEQDLKQITDKCQISEVIDKIINSNQDKVQEYKSGKTRLYGFFVGEVMKLTKGKASPDVVNSILSERLG

Sequences:

>Translated_473_residues
MTKEDWEAVIGLEVHAQVSSNTKLFSSSSTEFGAEHNTQVSLVDAAMPGTLPILNYYCIEQAIRTGLALSAEINKYSYFD
RKNYFYPDLPQGYQITQFFEPIVKNGRVFINDNEKEIRIARIHLEQDAGKSVHEESKTYVDLNRAGVALIEIVSEPDLRS
SAEAAECMKKLRQILRYTGSCDGDMEKGSLRCDANVSVRLKGSNTFGTRCEIKNLNSIRHIVQAIDYEIQRQIEILESGE
EISQDTLLFDVASGKTKVMRSKEDASDYRYFPEPDLLPVEVSQEKIDLIQSSLPELPDQKKLRYIEELGINEYDANVITS
DKAIADYFEELIKKHDSKLAVTWLTVELFGRLNKANIDIVSSPIKANALSELLDFIVDGTISAKLGKQVFDIMFETGKPA
SLIIEEQDLKQITDKCQISEVIDKIINSNQDKVQEYKSGKTRLYGFFVGEVMKLTKGKASPDVVNSILSERLG
>Mature_472_residues
TKEDWEAVIGLEVHAQVSSNTKLFSSSSTEFGAEHNTQVSLVDAAMPGTLPILNYYCIEQAIRTGLALSAEINKYSYFDR
KNYFYPDLPQGYQITQFFEPIVKNGRVFINDNEKEIRIARIHLEQDAGKSVHEESKTYVDLNRAGVALIEIVSEPDLRSS
AEAAECMKKLRQILRYTGSCDGDMEKGSLRCDANVSVRLKGSNTFGTRCEIKNLNSIRHIVQAIDYEIQRQIEILESGEE
ISQDTLLFDVASGKTKVMRSKEDASDYRYFPEPDLLPVEVSQEKIDLIQSSLPELPDQKKLRYIEELGINEYDANVITSD
KAIADYFEELIKKHDSKLAVTWLTVELFGRLNKANIDIVSSPIKANALSELLDFIVDGTISAKLGKQVFDIMFETGKPAS
LIIEEQDLKQITDKCQISEVIDKIINSNQDKVQEYKSGKTRLYGFFVGEVMKLTKGKASPDVVNSILSERLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=40.2834008097166, Blast_Score=348, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI115534094, Length=305, Percent_Identity=41.3114754098361, Blast_Score=225, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=502, Percent_Identity=31.6733067729084, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI45550808, Length=487, Percent_Identity=37.5770020533881, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: NA

Molecular weight: Translated: 53261; Mature: 53130

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEDWEAVIGLEVHAQVSSNTKLFSSSSTEFGAEHNTQVSLVDAAMPGTLPILNYYCIE
CCCCCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
QAIRTGLALSAEINKYSYFDRKNYFYPDLPQGYQITQFFEPIVKNGRVFINDNEKEIRIA
HHHHHCCEEEEECCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCEEEEE
RIHLEQDAGKSVHEESKTYVDLNRAGVALIEIVSEPDLRSSAEAAECMKKLRQILRYTGS
EEEEECCCCCHHHHCCCCEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
CDGDMEKGSLRCDANVSVRLKGSNTFGTRCEIKNLNSIRHIVQAIDYEIQRQIEILESGE
CCCCCCCCCEEECCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
EISQDTLLFDVASGKTKVMRSKEDASDYRYFPEPDLLPVEVSQEKIDLIQSSLPELPDQK
HHCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCHH
KLRYIEELGINEYDANVITSDKAIADYFEELIKKHDSKLAVTWLTVELFGRLNKANIDIV
HHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCCCCEEEE
SSPIKANALSELLDFIVDGTISAKLGKQVFDIMFETGKPASLIIEEQDLKQITDKCQISE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VIDKIINSNQDKVQEYKSGKTRLYGFFVGEVMKLTKGKASPDVVNSILSERLG
HHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEDWEAVIGLEVHAQVSSNTKLFSSSSTEFGAEHNTQVSLVDAAMPGTLPILNYYCIE
CCCCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
QAIRTGLALSAEINKYSYFDRKNYFYPDLPQGYQITQFFEPIVKNGRVFINDNEKEIRIA
HHHHHCCEEEEECCHHEEECCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEECCCCEEEEE
RIHLEQDAGKSVHEESKTYVDLNRAGVALIEIVSEPDLRSSAEAAECMKKLRQILRYTGS
EEEEECCCCCHHHHCCCCEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCC
CDGDMEKGSLRCDANVSVRLKGSNTFGTRCEIKNLNSIRHIVQAIDYEIQRQIEILESGE
CCCCCCCCCEEECCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
EISQDTLLFDVASGKTKVMRSKEDASDYRYFPEPDLLPVEVSQEKIDLIQSSLPELPDQK
HHCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCHH
KLRYIEELGINEYDANVITSDKAIADYFEELIKKHDSKLAVTWLTVELFGRLNKANIDIV
HHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCCCCEEEE
SSPIKANALSELLDFIVDGTISAKLGKQVFDIMFETGKPASLIIEEQDLKQITDKCQISE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VIDKIINSNQDKVQEYKSGKTRLYGFFVGEVMKLTKGKASPDVVNSILSERLG
HHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA