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The map label for this gene is map [H]

Identifier: 225629942

GI number: 225629942

Start: 85762

End: 86544

Strand: Reverse

Name: map [H]

Synonym: WRi_000870

Alternate gene names: 225629942

Gene position: 86544-85762 (Counterclockwise)

Preceding gene: 225629943

Following gene: 225629940

Centisome position: 5.99

GC content: 37.8

Gene sequence:

>783_bases
ATGAACATAACTATACACTCACAAGAAGATTTTGAATTTATGCGCAAGGCTGGCAGGCTTGCTGCTAAAACTCTTGATTT
CATTGCACCGTACGTAAAGGTAGGGGTGACAACTAATGAGTTAAATGATCTATGTCATGATTTTATAATTAATGCAGGTG
CAATTCCAGCACCACTAAACTATAGGGGGTATCCAAAATCGATTTGCACTTCAAAAAATGCAGTTGTGTGCCATGGTGTT
CCCGATAATAAACCACTTAAAGATGGAGATATTTTAAATATTGATGTCACGGTGATTTTAAATGGCTGGCATGGTGATAC
AAGTCGCATGTTTTGGGTTGGCAAGCCATCAATAAAAGCAAAACGCTTGTGTGACGCTACTTATCATGCATTGATGGAAG
CAATCAAACAAGTTAAGCCCGGCAATAAGTTAAATGAAATAGGGCTTGCTATAGAGAAATATATTAGAGATTTTGGATAT
TCTATCGTACGCAGCTACTGCGGACATGGTATAGGAAAAGTCTTTCATGCTCCACCAAATGTAGTGCATTTCTATGATCA
AGATGAAAATCTCGTCTTGAAGGAAGGTATGTTTTTTACAATAGAGCCAATGATTAATGCTGGAAAACATGAGACTCTGC
TCAGTAAACTCGATGACTGGACAGTAACAACACGCGATCTTTCACTTTCTGCTCAATTTGAGCATACGATTGGAGTAACA
AAAGATGGTGCTGAAATATTCACATTATCACCTAAGAATTGGCATTTCCCACCTTATAATTAG

Upstream 100 bases:

>100_bases
TTGACACTAGGATCCATAGGACTTATTTTTATAATATGTAAAATTATTATGTTTATGTACTTGCGAGTAAGCGTACCAAT
TACAATAAGACATGGAAAAT

Downstream 100 bases:

>100_bases
ATTTTTTTAAATTCTGCTATACTGCAATTATACTTGGTTTACTTTAGGTGATTTTGAAGAAAGCTCACCCTTAATTTGCG
ATATAGCAACTAGCCCAATT

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNITIHSQEDFEFMRKAGRLAAKTLDFIAPYVKVGVTTNELNDLCHDFIINAGAIPAPLNYRGYPKSICTSKNAVVCHGV
PDNKPLKDGDILNIDVTVILNGWHGDTSRMFWVGKPSIKAKRLCDATYHALMEAIKQVKPGNKLNEIGLAIEKYIRDFGY
SIVRSYCGHGIGKVFHAPPNVVHFYDQDENLVLKEGMFFTIEPMINAGKHETLLSKLDDWTVTTRDLSLSAQFEHTIGVT
KDGAEIFTLSPKNWHFPPYN

Sequences:

>Translated_260_residues
MNITIHSQEDFEFMRKAGRLAAKTLDFIAPYVKVGVTTNELNDLCHDFIINAGAIPAPLNYRGYPKSICTSKNAVVCHGV
PDNKPLKDGDILNIDVTVILNGWHGDTSRMFWVGKPSIKAKRLCDATYHALMEAIKQVKPGNKLNEIGLAIEKYIRDFGY
SIVRSYCGHGIGKVFHAPPNVVHFYDQDENLVLKEGMFFTIEPMINAGKHETLLSKLDDWTVTTRDLSLSAQFEHTIGVT
KDGAEIFTLSPKNWHFPPYN
>Mature_260_residues
MNITIHSQEDFEFMRKAGRLAAKTLDFIAPYVKVGVTTNELNDLCHDFIINAGAIPAPLNYRGYPKSICTSKNAVVCHGV
PDNKPLKDGDILNIDVTVILNGWHGDTSRMFWVGKPSIKAKRLCDATYHALMEAIKQVKPGNKLNEIGLAIEKYIRDFGY
SIVRSYCGHGIGKVFHAPPNVVHFYDQDENLVLKEGMFFTIEPMINAGKHETLLSKLDDWTVTTRDLSLSAQFEHTIGVT
KDGAEIFTLSPKNWHFPPYN

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=249, Percent_Identity=48.5943775100402, Blast_Score=234, Evalue=4e-62,
Organism=Homo sapiens, GI40385867, Length=250, Percent_Identity=42, Blast_Score=200, Evalue=1e-51,
Organism=Escherichia coli, GI1786364, Length=251, Percent_Identity=58.9641434262948, Blast_Score=298, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71996291, Length=247, Percent_Identity=44.1295546558704, Blast_Score=207, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6323273, Length=252, Percent_Identity=45.6349206349206, Blast_Score=227, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21355531, Length=253, Percent_Identity=49.4071146245059, Blast_Score=237, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24583427, Length=244, Percent_Identity=43.8524590163934, Blast_Score=215, Evalue=3e-56,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 29166; Mature: 29166

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITIHSQEDFEFMRKAGRLAAKTLDFIAPYVKVGVTTNELNDLCHDFIINAGAIPAPLN
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
YRGYPKSICTSKNAVVCHGVPDNKPLKDGDILNIDVTVILNGWHGDTSRMFWVGKPSIKA
CCCCCHHHHCCCCCEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCHH
KRLCDATYHALMEAIKQVKPGNKLNEIGLAIEKYIRDFGYSIVRSYCGHGIGKVFHAPPN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
VVHFYDQDENLVLKEGMFFTIEPMINAGKHETLLSKLDDWTVTTRDLSLSAQFEHTIGVT
EEEEEECCCCEEEECCCEEEEECCCCCCCHHHHHHHCCCCEEEEECEEEEEEEHEECCCC
KDGAEIFTLSPKNWHFPPYN
CCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MNITIHSQEDFEFMRKAGRLAAKTLDFIAPYVKVGVTTNELNDLCHDFIINAGAIPAPLN
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
YRGYPKSICTSKNAVVCHGVPDNKPLKDGDILNIDVTVILNGWHGDTSRMFWVGKPSIKA
CCCCCHHHHCCCCCEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEEECCCCHH
KRLCDATYHALMEAIKQVKPGNKLNEIGLAIEKYIRDFGYSIVRSYCGHGIGKVFHAPPN
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
VVHFYDQDENLVLKEGMFFTIEPMINAGKHETLLSKLDDWTVTTRDLSLSAQFEHTIGVT
EEEEEECCCCEEEECCCEEEEECCCCCCCHHHHHHHCCCCEEEEECEEEEEEEHEECCCC
KDGAEIFTLSPKNWHFPPYN
CCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]