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The map label for this gene is mraW

Identifier: 225629940

GI number: 225629940

Start: 83387

End: 84268

Strand: Reverse

Name: mraW

Synonym: WRi_000850

Alternate gene names: 225629940

Gene position: 84268-83387 (Counterclockwise)

Preceding gene: 225629942

Following gene: 225629939

Centisome position: 5.83

GC content: 36.96

Gene sequence:

>882_bases
ATGACACACACTCCAGTTTTGTTAAAAGAGATGCTATCACTACTATCACCGCAGGATGGTGGCATATACGTGGACGCCAC
ATTTGGAGCTGGAGGGTATAGCAAAGCAATATTGGAGTCAGCTGATTGCAAAGTATATGCAATTGATAGAGATGAAACGG
TAACTAAGTTTTATGATGATTTAAGCGTTAGATATCCAGATAGAATAAAACTATTCATTGAGAAGTTTAGTAATATTAAA
AATTTATTAGATAGCAACAATATTGAAGGTATCGATGGAATAGTTTTTGACATCGGAGTTTCATCTATGCAGCTTGATAA
CGGGGATAGAGGGTTCTCATTTTTACATGATGGTCCACTCGATATGAGTATGGATAACTCTTCTTATATAAACGCTTCAA
CGTTTGTTAACGCTTTACGCGAAGAAGAAATCGCAAATACTATATATAACTACGGAGGCGAACGTCATTCTCGCAAAATC
GCAAGAGCAATAGTAAATGCACGGAAGAAGAAAACTATCAAAACTACATTTGAGCTTGCGGATATTGTACGTTCCGTGGT
ATTTCGTGGAAAAAGCAAAATTGATCCTGCAACCAGAACATTTCAGGCAATCAGAATATGGGTAAACGATGAGCTGGGAG
AACTTGAAAAGGGTATTAAAGCTGCATCTGAGATTTTAAGTGAGAATGGCAAATTAATTGTCGTCACCTTTCATTCCTTA
GAAGATCGTATAGTCAAAACTTTTTTTAAAGATTTATGTGCTACTGATTGCAAGACATTCTCTCTGCTAAATAAAAAAGT
GATCGAAGCAAGTATAGAAGAAGTAAGTGCAAATCCGCGTTCACGCTCAGCAAAACTAAGAGCTATACAGAGGTTGTCGT
GA

Upstream 100 bases:

>100_bases
TACCATATTTTGGCAAGAAAATCTATGTACATAAATAAAATTGATGTGAAGAGTAGAGTAGAGTAGGATATCTACACCAA
CTGTACTTAAATTTTTTGAT

Downstream 100 bases:

>100_bases
GAACTTTCTGCATCATCTCAATAGTTATGTTTTTTCTTAGCATTGTAGGACTATTTAAGGTAAAATTACATGTTCAGTCG
TTAAATAGAGAGCTAATAAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTHTPVLLKEMLSLLSPQDGGIYVDATFGAGGYSKAILESADCKVYAIDRDETVTKFYDDLSVRYPDRIKLFIEKFSNIK
NLLDSNNIEGIDGIVFDIGVSSMQLDNGDRGFSFLHDGPLDMSMDNSSYINASTFVNALREEEIANTIYNYGGERHSRKI
ARAIVNARKKKTIKTTFELADIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSL
EDRIVKTFFKDLCATDCKTFSLLNKKVIEASIEEVSANPRSRSAKLRAIQRLS

Sequences:

>Translated_293_residues
MTHTPVLLKEMLSLLSPQDGGIYVDATFGAGGYSKAILESADCKVYAIDRDETVTKFYDDLSVRYPDRIKLFIEKFSNIK
NLLDSNNIEGIDGIVFDIGVSSMQLDNGDRGFSFLHDGPLDMSMDNSSYINASTFVNALREEEIANTIYNYGGERHSRKI
ARAIVNARKKKTIKTTFELADIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSL
EDRIVKTFFKDLCATDCKTFSLLNKKVIEASIEEVSANPRSRSAKLRAIQRLS
>Mature_292_residues
THTPVLLKEMLSLLSPQDGGIYVDATFGAGGYSKAILESADCKVYAIDRDETVTKFYDDLSVRYPDRIKLFIEKFSNIKN
LLDSNNIEGIDGIVFDIGVSSMQLDNGDRGFSFLHDGPLDMSMDNSSYINASTFVNALREEEIANTIYNYGGERHSRKIA
RAIVNARKKKTIKTTFELADIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSLE
DRIVKTFFKDLCATDCKTFSLLNKKVIEASIEEVSANPRSRSAKLRAIQRLS

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=40.1785714285714, Blast_Score=230, Evalue=9e-61,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=44.3850267379679, Blast_Score=161, Evalue=8e-40,
Organism=Escherichia coli, GI1786270, Length=310, Percent_Identity=39.3548387096774, Blast_Score=208, Evalue=4e-55,
Organism=Drosophila melanogaster, GI62472493, Length=325, Percent_Identity=38.1538461538462, Blast_Score=198, Evalue=3e-51,
Organism=Drosophila melanogaster, GI28571637, Length=325, Percent_Identity=38.1538461538462, Blast_Score=198, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_WOLWR (C0R598)

Other databases:

- EMBL:   CP001391
- RefSeq:   YP_002726731.1
- ProteinModelPortal:   C0R598
- SMR:   C0R598
- EnsemblBacteria:   EBWOLT00000002454
- GeneID:   7668836
- GenomeReviews:   CP001391_GR
- KEGG:   wri:WRi_000850
- GeneTree:   EBGT00050000031416
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 32796; Mature: 32665

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: BINDING 49-49 BINDING 76-76 BINDING 97-97 BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHTPVLLKEMLSLLSPQDGGIYVDATFGAGGYSKAILESADCKVYAIDRDETVTKFYDD
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
LSVRYPDRIKLFIEKFSNIKNLLDSNNIEGIDGIVFDIGVSSMQLDNGDRGFSFLHDGPL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCC
DMSMDNSSYINASTFVNALREEEIANTIYNYGGERHSRKIARAIVNARKKKTIKTTFELA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSL
HHHHHHHHCCCCCCCHHHHHEEHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCH
EDRIVKTFFKDLCATDCKTFSLLNKKVIEASIEEVSANPRSRSAKLRAIQRLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
THTPVLLKEMLSLLSPQDGGIYVDATFGAGGYSKAILESADCKVYAIDRDETVTKFYDD
CCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHH
LSVRYPDRIKLFIEKFSNIKNLLDSNNIEGIDGIVFDIGVSSMQLDNGDRGFSFLHDGPL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCC
DMSMDNSSYINASTFVNALREEEIANTIYNYGGERHSRKIARAIVNARKKKTIKTTFELA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIVRSVVFRGKSKIDPATRTFQAIRIWVNDELGELEKGIKAASEILSENGKLIVVTFHSL
HHHHHHHHCCCCCCCHHHHHEEHEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCH
EDRIVKTFFKDLCATDCKTFSLLNKKVIEASIEEVSANPRSRSAKLRAIQRLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA