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The map label for this gene is pth [H]

Identifier: 225629936

GI number: 225629936

Start: 81182

End: 81730

Strand: Reverse

Name: pth [H]

Synonym: WRi_000810

Alternate gene names: 225629936

Gene position: 81730-81182 (Counterclockwise)

Preceding gene: 225629937

Following gene: 225629927

Centisome position: 5.65

GC content: 34.06

Gene sequence:

>549_bases
GTGCACCTGATAGTTGGGCTTGGTAACCCTGGTAGTCAATATGAGTTAACTCATCATAATATCGGCTTCATCACCGTTGA
TGCAATCTGCAAATATTGGGGTTTTCAGTTGTTCTCTAAAAAAGCCGATTATCTGATAACCTCTGGCATGATTAATGATA
ATAAAATCATGTTAATAAAGCCTTATTCATTTATGAATAATTCAGGCATCCCTGTTGCAAAAATACGAAACTTTTACAAA
TTATCGCTAGATAATATCGTTGTCATACACGATGACGCTGATTTGGAACTTGGAAGAATAAAAGTAAAGAAAGGTGGTAG
CTCTGCTGGACATAATGGACTTAAATCCATAGATAGTTTTATTGGCAACGATTATTGGCGCTTGAGATTTGGAGTGGGTA
GACCTGAAGATCAAAGAAGCTTAGCAGATTATGTGTTATCAAAATTTTCAAATTTTGATAATGTTACTCCCTTAGTGGAA
AAAATAGCAAAAAATATACACTTAATGCTGCAAGGAGATAATACAGCTTTTATCAATTTGATTGTATAA

Upstream 100 bases:

>100_bases
GTGTTAAGTTTGTAGCTCATGAAGAAGAAAATTTTACCATTGTTACAATTTCTGCTGCCGATAGTGACGTTGAAGAACCT
CAAGCAGAAACAGAGGAATA

Downstream 100 bases:

>100_bases
TATTACGAAACACAGGGAGTGTATGGTCCCAGAGTGTGATGGTGTGGATTTAGTATGAACAATTCAGGAGATTTTGTCCA
CTGTAAAGATATAAACTCAA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MHLIVGLGNPGSQYELTHHNIGFITVDAICKYWGFQLFSKKADYLITSGMINDNKIMLIKPYSFMNNSGIPVAKIRNFYK
LSLDNIVVIHDDADLELGRIKVKKGGSSAGHNGLKSIDSFIGNDYWRLRFGVGRPEDQRSLADYVLSKFSNFDNVTPLVE
KIAKNIHLMLQGDNTAFINLIV

Sequences:

>Translated_182_residues
MHLIVGLGNPGSQYELTHHNIGFITVDAICKYWGFQLFSKKADYLITSGMINDNKIMLIKPYSFMNNSGIPVAKIRNFYK
LSLDNIVVIHDDADLELGRIKVKKGGSSAGHNGLKSIDSFIGNDYWRLRFGVGRPEDQRSLADYVLSKFSNFDNVTPLVE
KIAKNIHLMLQGDNTAFINLIV
>Mature_182_residues
MHLIVGLGNPGSQYELTHHNIGFITVDAICKYWGFQLFSKKADYLITSGMINDNKIMLIKPYSFMNNSGIPVAKIRNFYK
LSLDNIVVIHDDADLELGRIKVKKGGSSAGHNGLKSIDSFIGNDYWRLRFGVGRPEDQRSLADYVLSKFSNFDNVTPLVE
KIAKNIHLMLQGDNTAFINLIV

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=158, Percent_Identity=31.0126582278481, Blast_Score=92, Evalue=3e-19,
Organism=Escherichia coli, GI1787455, Length=151, Percent_Identity=39.7350993377483, Blast_Score=87, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 20437; Mature: 20437

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLIVGLGNPGSQYELTHHNIGFITVDAICKYWGFQLFSKKADYLITSGMINDNKIMLIK
CEEEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEECCEECCCEEEEEC
PYSFMNNSGIPVAKIRNFYKLSLDNIVVIHDDADLELGRIKVKKGGSSAGHNGLKSIDSF
CHHHCCCCCCCHHHHHHEEEEECCCEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHH
IGNDYWRLRFGVGRPEDQRSLADYVLSKFSNFDNVTPLVEKIAKNIHLMLQGDNTAFINL
HCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEEECCCEEEEEE
IV
EC
>Mature Secondary Structure
MHLIVGLGNPGSQYELTHHNIGFITVDAICKYWGFQLFSKKADYLITSGMINDNKIMLIK
CEEEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHCCCCCEEEECCEECCCEEEEEC
PYSFMNNSGIPVAKIRNFYKLSLDNIVVIHDDADLELGRIKVKKGGSSAGHNGLKSIDSF
CHHHCCCCCCCHHHHHHEEEEECCCEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHH
IGNDYWRLRFGVGRPEDQRSLADYVLSKFSNFDNVTPLVEKIAKNIHLMLQGDNTAFINL
HCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEEEECCCEEEEEE
IV
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA