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The map label for this gene is pyrF [H]

Identifier: 222527451

GI number: 222527451

Start: 5260550

End: 5261278

Strand: Direct

Name: pyrF [H]

Synonym: Chy400_4242

Alternate gene names: 222527451

Gene position: 5260550-5261278 (Clockwise)

Preceding gene: 222527450

Following gene: 222527452

Centisome position: 99.84

GC content: 60.77

Gene sequence:

>729_bases
ATGAGTCTCGCGCCACGCGAACGGATACTGGTTGCGCTCGATACACCCGATCTGGATGCAGCAGTTGCTTTAGCAACCCG
GTTGCGCGGGCAGGTTGGCGGCTATAAAGTCGGCCTGGAAATCTGCTCTGCGGTCGGCGTACCCCAGATTGTATCCGCCA
TGACTGCGGTTGGCGGAAGTGTCTTTCTCGATCTCAAACTGCACGACATTCCGAACACGGTCGCCGGTGCAGTCCGCGCC
GTCTGCAACCTCGGCCCGGCAGTCCAGATGTTGACACTCCACTGTAGTGGAGGTAGCGCAATGCTCCGCGCCGCAGTCAA
TGCAGCCCAAAGCATGCCCTACCGTCCACTGCTGTTGGGCGTTACCGTGCTCACCAGTCTCGATGCCACAGCACTCGCCG
GCGAACTGCGTGTCACGTCGGACATCACCGACTACGTAGTTCATCTGGCTCGCATGGCGCAGGATTGTGGTCTCGACGGC
GTGGTAGCTTCGCCGCACGAAGTGAGTGCCATTCGTACTGCCTGCCCTCACGTGCGGATAGTCACCCCCGGTATTCGCCC
ACAGTGGGCCGCAGAAGGTGACCAGCGTCGCGTCATGACACCGGCAGCCGCCCTACGCGCTGGTGCCGACTATCTGGTCA
TCGGACGACCAATTACTGCGCCACCGGCAATCATCGGCGATCCGGTCACCGCAGTCGCACGGATTCTTGCAGAATTGGAA
CAGGAATGA

Upstream 100 bases:

>100_bases
GCCTGTATCTGGCCGCAACCATTATTGGGGTTGCGGTGTATGTTTTGATCCACACCTGATAACGTTGTTCGACCTGAGAT
AGACTGGAGCGGTTCATCGT

Downstream 100 bases:

>100_bases
TAGAAGCTTATGACCGAGGCGAATCTCTTAACCATCCTTCAGGAATTAGGAGCAATTGTCACCAACGACCATATCGTGTA
CACCTCTGGGCGCCACGGCA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSLAPRERILVALDTPDLDAAVALATRLRGQVGGYKVGLEICSAVGVPQIVSAMTAVGGSVFLDLKLHDIPNTVAGAVRA
VCNLGPAVQMLTLHCSGGSAMLRAAVNAAQSMPYRPLLLGVTVLTSLDATALAGELRVTSDITDYVVHLARMAQDCGLDG
VVASPHEVSAIRTACPHVRIVTPGIRPQWAAEGDQRRVMTPAAALRAGADYLVIGRPITAPPAIIGDPVTAVARILAELE
QE

Sequences:

>Translated_242_residues
MSLAPRERILVALDTPDLDAAVALATRLRGQVGGYKVGLEICSAVGVPQIVSAMTAVGGSVFLDLKLHDIPNTVAGAVRA
VCNLGPAVQMLTLHCSGGSAMLRAAVNAAQSMPYRPLLLGVTVLTSLDATALAGELRVTSDITDYVVHLARMAQDCGLDG
VVASPHEVSAIRTACPHVRIVTPGIRPQWAAEGDQRRVMTPAAALRAGADYLVIGRPITAPPAIIGDPVTAVARILAELE
QE
>Mature_241_residues
SLAPRERILVALDTPDLDAAVALATRLRGQVGGYKVGLEICSAVGVPQIVSAMTAVGGSVFLDLKLHDIPNTVAGAVRAV
CNLGPAVQMLTLHCSGGSAMLRAAVNAAQSMPYRPLLLGVTVLTSLDATALAGELRVTSDITDYVVHLARMAQDCGLDGV
VASPHEVSAIRTACPHVRIVTPGIRPQWAAEGDQRRVMTPAAALRAGADYLVIGRPITAPPAIIGDPVTAVARILAELEQ
E

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=237, Percent_Identity=43.8818565400844, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25105; Mature: 24973

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAPRERILVALDTPDLDAAVALATRLRGQVGGYKVGLEICSAVGVPQIVSAMTAVGGS
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCE
VFLDLKLHDIPNTVAGAVRAVCNLGPAVQMLTLHCSGGSAMLRAAVNAAQSMPYRPLLLG
EEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHH
VTVLTSLDATALAGELRVTSDITDYVVHLARMAQDCGLDGVVASPHEVSAIRTACPHVRI
HHHHHHCCHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCEEE
VTPGIRPQWAAEGDQRRVMTPAAALRAGADYLVIGRPITAPPAIIGDPVTAVARILAELE
ECCCCCCCCCCCCCCCEEECHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
QE
CC
>Mature Secondary Structure 
SLAPRERILVALDTPDLDAAVALATRLRGQVGGYKVGLEICSAVGVPQIVSAMTAVGGS
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCCE
VFLDLKLHDIPNTVAGAVRAVCNLGPAVQMLTLHCSGGSAMLRAAVNAAQSMPYRPLLLG
EEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCHHHHH
VTVLTSLDATALAGELRVTSDITDYVVHLARMAQDCGLDGVVASPHEVSAIRTACPHVRI
HHHHHHCCHHHHHCCEEEHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCCEEE
VTPGIRPQWAAEGDQRRVMTPAAALRAGADYLVIGRPITAPPAIIGDPVTAVARILAELE
ECCCCCCCCCCCCCCCEEECHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
QE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA