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The map label for this gene is topA [H]

Identifier: 222527443

GI number: 222527443

Start: 5251288

End: 5253618

Strand: Reverse

Name: topA [H]

Synonym: Chy400_4234

Alternate gene names: 222527443

Gene position: 5253618-5251288 (Counterclockwise)

Preceding gene: 222527455

Following gene: 222527441

Centisome position: 99.71

GC content: 58.34

Gene sequence:

>2331_bases
ATGGGTGAAAAAGTTGTGATTGTTGAGTCGCCGGCAAAGGCGCGGACGATTCAAAAATATCTGGGCAAAGGCTACAAGGT
TGCTTCGAGTATGGGGCACGTGCGTGATCTGCCCAAGAGCGGCCTTGCAATCGATATTGAACACGATTTTGCGCCTTCGT
ATGAGATTGTCAAGCCAAAGGTTGTCAGCGAGTTGAGCCAGGCTCTGCGCAACGCCGAGGCGGTCTACCTGGCGACCGAC
CCGGATCGGGAAGGCGAGGCGATTGCCTGGCATATTACGCAGGCGGTCAAGCTGCCGAAGAAGACGCCGGTGTATCGGGT
GGTCTTCCAGGAGATTACCCGCAATGCTGTGCAACAGGCGCTGCAACAACCGCGCCAGATCAATCAGAATCTGGTGGAAG
CGCAGCAGGCCCGGCGCGTACTTGATCGGCTGGTCGGCTACCAGTTAAGCCCGTTGCTTTGGGATAAAGTAAAGCGAGGC
TTAAGCGCCGGACGTGTGCAGTCGGTTGCCGTGCGCTTGATTGTCGAGCGTGAACGGGAGATCGAGCAGTTTCAACCGCA
GGAATACTGGACTATCGAGGCCGATCTGCTGAAAGATGCCGGTGTCGCCCCGCGTGATCTCTTCCGGGCGGTGCTGATTG
AGCGCGATGGGAAGAAGCTTGAAAAGTTTTCAATCGAGAATCGCGAACAGGCCGAGGCGATTGTGGCCGATCTGCAGGGG
GCTGCCTATACCGTCGTGCGGGTGACGCGGCGCGACAAGCGGCGCAGCCCGGCTCCTCCCTTTACCACCAGCACCCTGCA
ACAAGAGGCAGCGCGCAAGTTGGGCTTTAGTGCCAAGAAGACGATGACCCTTGCCCAGCGCCTGTACGAAGGCGTTGATA
TTGGTGGTGAGGACGGGATGGTCGGTCTGATTACCTATATGCGTACCGACAGCGTCCAGGTTGCTGCGGAAGCGCAGACT
GAAGCGCGTGATGTGATTGGCAAGCGTTTCGGCAAAGAGTATCTGCCCGATCAGCCGCCGGTGTACAAGACAAAGGCGAA
GGGTGCCCAGGAGGCACACGAGGCGATTCGACCAACCAGCAGCGCCCGCTTGCCCGAACAGTTGAGTGGAAAGCTTGAAA
ACGATTTGTGGCGGCTCTACGATCTGATCTGGAAGCGGTTTATTGCCTCGCAGATGGCACCGGCAGTCTTCGATAGCACA
ACCGTTGACATTGCAGCAACACCGGTCGTTGCCGGTGCGCCGGCCTACCTCTTCCGCGCTACCGGATCGGTGCTCAAGTT
CCCCGGTTTTCTCGCCGTCTATAACGTCAGCCTCGATGAAGGCGAAGAAGACGAAGACAGTGAACGGCGTTTGCCGCCGC
TGGCTGAAGGGGAAGCGTTGCAACTGGTTGAGCTGTTGCCGATCCAGCACTTCACCGAACCACCGCCCCGCTACACCGAG
GCCAGCCTGGTGAAAGAGCTGGAGCGGCTCGGCATCGGACGGCCCAGTACCTACGCCTCGATACTCTCGACGATTCAAGA
ACGCGAATATGTCGAGATGGTCGATAAAAAGCTGATCCCGACAACGCTAGGGCGGGTGGTGACCGATTTGCTGGTGGAGC
ATTTCGGCAACATTGTCGATTACGATTTTACCTCGGCCCTGGAACAGCAGCTCGATGATATTGCGGAAGGATCGAAGAAG
TGGGTGCCGGTGCTGCGCGAGTTCTACGGCCCCTTCCGTTCAACGCTTGAACAGGCGCAGCGCCAGATGCGCAATGTCAA
ACGCGAAGAGATCGTCACCGATCTCGACTGTCCGAAGTGTGGTCAGGGGAAACTGGTGATTCGCTTTGGCCGGAACGGTG
AATTTCTGGCCTGCTCGCGCTACAACCGCGAAGGCGACGGTGAGTCGTGCGATTTTACCAGTGATTTTCACCGTGATGAA
CAAGGTCAGATTGTGATCGACAAAGCCAGTGCGCCTGAGACGAGCGATGTGCTGTGTAATGTCTGTGGCCGGCCCATGGT
GATCAAAAAGAGCCGCTTCGGCCCCTTCCTCGGCTGTTCGGGCTACCCGGAATGCAACAACACCCGCCGGATCGGGCGTG
ACGGCAAACCAGTGCCGCTTCCCGAACCGACCGGTGTCCAATGCCCGAAGTGCGGCGAGGGTGAATTGCTGCGCCGGCGA
GGAAAATTTGGCCGCCCGTTCTACGGCTGTTCGCGCTACCCGAAGTGTGACTACATCACCAATACGCTCGATGAGGCGCA
GGCGGGTGAGGCACCGGCAGAGCCGGCAAAGAACGAGCCTGCGTTGCCCTCACCTTCCCGTTCATCAAAGCGCACCCGCA
AGAGTGCTTGA

Upstream 100 bases:

>100_bases
CCCGAATCGTTCTTGACAAGCTGGCACGGGCCGCGTATAGTGCGAAAGCAAGATGGACGAGGCTGAAAGACGGACATTAC
ATAATAATAGGAATTATCAC

Downstream 100 bases:

>100_bases
GTACTCGTCTGGATCATCTATATTGGTTTTACGGTATGGGCAGGCGGATATAACATCCTTGCCTGCCCTGTTCACATCTG
GGTACAAGCCCGCCCTTCTG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MGEKVVIVESPAKARTIQKYLGKGYKVASSMGHVRDLPKSGLAIDIEHDFAPSYEIVKPKVVSELSQALRNAEAVYLATD
PDREGEAIAWHITQAVKLPKKTPVYRVVFQEITRNAVQQALQQPRQINQNLVEAQQARRVLDRLVGYQLSPLLWDKVKRG
LSAGRVQSVAVRLIVEREREIEQFQPQEYWTIEADLLKDAGVAPRDLFRAVLIERDGKKLEKFSIENREQAEAIVADLQG
AAYTVVRVTRRDKRRSPAPPFTTSTLQQEAARKLGFSAKKTMTLAQRLYEGVDIGGEDGMVGLITYMRTDSVQVAAEAQT
EARDVIGKRFGKEYLPDQPPVYKTKAKGAQEAHEAIRPTSSARLPEQLSGKLENDLWRLYDLIWKRFIASQMAPAVFDST
TVDIAATPVVAGAPAYLFRATGSVLKFPGFLAVYNVSLDEGEEDEDSERRLPPLAEGEALQLVELLPIQHFTEPPPRYTE
ASLVKELERLGIGRPSTYASILSTIQEREYVEMVDKKLIPTTLGRVVTDLLVEHFGNIVDYDFTSALEQQLDDIAEGSKK
WVPVLREFYGPFRSTLEQAQRQMRNVKREEIVTDLDCPKCGQGKLVIRFGRNGEFLACSRYNREGDGESCDFTSDFHRDE
QGQIVIDKASAPETSDVLCNVCGRPMVIKKSRFGPFLGCSGYPECNNTRRIGRDGKPVPLPEPTGVQCPKCGEGELLRRR
GKFGRPFYGCSRYPKCDYITNTLDEAQAGEAPAEPAKNEPALPSPSRSSKRTRKSA

Sequences:

>Translated_776_residues
MGEKVVIVESPAKARTIQKYLGKGYKVASSMGHVRDLPKSGLAIDIEHDFAPSYEIVKPKVVSELSQALRNAEAVYLATD
PDREGEAIAWHITQAVKLPKKTPVYRVVFQEITRNAVQQALQQPRQINQNLVEAQQARRVLDRLVGYQLSPLLWDKVKRG
LSAGRVQSVAVRLIVEREREIEQFQPQEYWTIEADLLKDAGVAPRDLFRAVLIERDGKKLEKFSIENREQAEAIVADLQG
AAYTVVRVTRRDKRRSPAPPFTTSTLQQEAARKLGFSAKKTMTLAQRLYEGVDIGGEDGMVGLITYMRTDSVQVAAEAQT
EARDVIGKRFGKEYLPDQPPVYKTKAKGAQEAHEAIRPTSSARLPEQLSGKLENDLWRLYDLIWKRFIASQMAPAVFDST
TVDIAATPVVAGAPAYLFRATGSVLKFPGFLAVYNVSLDEGEEDEDSERRLPPLAEGEALQLVELLPIQHFTEPPPRYTE
ASLVKELERLGIGRPSTYASILSTIQEREYVEMVDKKLIPTTLGRVVTDLLVEHFGNIVDYDFTSALEQQLDDIAEGSKK
WVPVLREFYGPFRSTLEQAQRQMRNVKREEIVTDLDCPKCGQGKLVIRFGRNGEFLACSRYNREGDGESCDFTSDFHRDE
QGQIVIDKASAPETSDVLCNVCGRPMVIKKSRFGPFLGCSGYPECNNTRRIGRDGKPVPLPEPTGVQCPKCGEGELLRRR
GKFGRPFYGCSRYPKCDYITNTLDEAQAGEAPAEPAKNEPALPSPSRSSKRTRKSA
>Mature_775_residues
GEKVVIVESPAKARTIQKYLGKGYKVASSMGHVRDLPKSGLAIDIEHDFAPSYEIVKPKVVSELSQALRNAEAVYLATDP
DREGEAIAWHITQAVKLPKKTPVYRVVFQEITRNAVQQALQQPRQINQNLVEAQQARRVLDRLVGYQLSPLLWDKVKRGL
SAGRVQSVAVRLIVEREREIEQFQPQEYWTIEADLLKDAGVAPRDLFRAVLIERDGKKLEKFSIENREQAEAIVADLQGA
AYTVVRVTRRDKRRSPAPPFTTSTLQQEAARKLGFSAKKTMTLAQRLYEGVDIGGEDGMVGLITYMRTDSVQVAAEAQTE
ARDVIGKRFGKEYLPDQPPVYKTKAKGAQEAHEAIRPTSSARLPEQLSGKLENDLWRLYDLIWKRFIASQMAPAVFDSTT
VDIAATPVVAGAPAYLFRATGSVLKFPGFLAVYNVSLDEGEEDEDSERRLPPLAEGEALQLVELLPIQHFTEPPPRYTEA
SLVKELERLGIGRPSTYASILSTIQEREYVEMVDKKLIPTTLGRVVTDLLVEHFGNIVDYDFTSALEQQLDDIAEGSKKW
VPVLREFYGPFRSTLEQAQRQMRNVKREEIVTDLDCPKCGQGKLVIRFGRNGEFLACSRYNREGDGESCDFTSDFHRDEQ
GQIVIDKASAPETSDVLCNVCGRPMVIKKSRFGPFLGCSGYPECNNTRRIGRDGKPVPLPEPTGVQCPKCGEGELLRRRG
KFGRPFYGCSRYPKCDYITNTLDEAQAGEAPAEPAKNEPALPSPSRSSKRTRKSA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=597, Percent_Identity=25.963149078727, Blast_Score=132, Evalue=2e-30,
Organism=Homo sapiens, GI4507635, Length=682, Percent_Identity=24.4868035190616, Blast_Score=124, Evalue=4e-28,
Organism=Escherichia coli, GI1787529, Length=830, Percent_Identity=40.1204819277108, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI48994931, Length=156, Percent_Identity=37.1794871794872, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1788061, Length=569, Percent_Identity=24.9560632688928, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=558, Percent_Identity=25.4480286738351, Blast_Score=135, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=568, Percent_Identity=25.1760563380282, Blast_Score=126, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=585, Percent_Identity=25.6410256410256, Blast_Score=107, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24585251, Length=718, Percent_Identity=26.0445682451253, Blast_Score=150, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24640096, Length=542, Percent_Identity=23.2472324723247, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 86766; Mature: 86635

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKVVIVESPAKARTIQKYLGKGYKVASSMGHVRDLPKSGLAIDIEHDFAPSYEIVKPK
CCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHCCHH
VVSELSQALRNAEAVYLATDPDREGEAIAWHITQAVKLPKKTPVYRVVFQEITRNAVQQA
HHHHHHHHHHCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LQQPRQINQNLVEAQQARRVLDRLVGYQLSPLLWDKVKRGLSAGRVQSVAVRLIVERERE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IEQFQPQEYWTIEADLLKDAGVAPRDLFRAVLIERDGKKLEKFSIENREQAEAIVADLQG
HHHCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCC
AAYTVVRVTRRDKRRSPAPPFTTSTLQQEAARKLGFSAKKTMTLAQRLYEGVDIGGEDGM
CEEEEHEEHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCC
VGLITYMRTDSVQVAAEAQTEARDVIGKRFGKEYLPDQPPVYKTKAKGAQEAHEAIRPTS
EEEHHHHHCCCEEEEHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHCCCC
SARLPEQLSGKLENDLWRLYDLIWKRFIASQMAPAVFDSTTVDIAATPVVAGAPAYLFRA
CCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEECCCCCCCCHHHHHH
TGSVLKFPGFLAVYNVSLDEGEEDEDSERRLPPLAEGEALQLVELLPIQHFTEPPPRYTE
CCCHHHCCCEEEEEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHH
ASLVKELERLGIGRPSTYASILSTIQEREYVEMVDKKLIPTTLGRVVTDLLVEHFGNIVD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YDFTSALEQQLDDIAEGSKKWVPVLREFYGPFRSTLEQAQRQMRNVKREEIVTDLDCPKC
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GQGKLVIRFGRNGEFLACSRYNREGDGESCDFTSDFHRDEQGQIVIDKASAPETSDVLCN
CCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCHHHHHHH
VCGRPMVIKKSRFGPFLGCSGYPECNNTRRIGRDGKPVPLPEPTGVQCPKCGEGELLRRR
HCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHC
GKFGRPFYGCSRYPKCDYITNTLDEAQAGEAPAEPAKNEPALPSPSRSSKRTRKSA
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
GEKVVIVESPAKARTIQKYLGKGYKVASSMGHVRDLPKSGLAIDIEHDFAPSYEIVKPK
CCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHCCHH
VVSELSQALRNAEAVYLATDPDREGEAIAWHITQAVKLPKKTPVYRVVFQEITRNAVQQA
HHHHHHHHHHCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LQQPRQINQNLVEAQQARRVLDRLVGYQLSPLLWDKVKRGLSAGRVQSVAVRLIVERERE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
IEQFQPQEYWTIEADLLKDAGVAPRDLFRAVLIERDGKKLEKFSIENREQAEAIVADLQG
HHHCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCC
AAYTVVRVTRRDKRRSPAPPFTTSTLQQEAARKLGFSAKKTMTLAQRLYEGVDIGGEDGM
CEEEEHEEHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCC
VGLITYMRTDSVQVAAEAQTEARDVIGKRFGKEYLPDQPPVYKTKAKGAQEAHEAIRPTS
EEEHHHHHCCCEEEEHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHCCCC
SARLPEQLSGKLENDLWRLYDLIWKRFIASQMAPAVFDSTTVDIAATPVVAGAPAYLFRA
CCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEEECCCCCCCCHHHHHH
TGSVLKFPGFLAVYNVSLDEGEEDEDSERRLPPLAEGEALQLVELLPIQHFTEPPPRYTE
CCCHHHCCCEEEEEEEECCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHH
ASLVKELERLGIGRPSTYASILSTIQEREYVEMVDKKLIPTTLGRVVTDLLVEHFGNIVD
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YDFTSALEQQLDDIAEGSKKWVPVLREFYGPFRSTLEQAQRQMRNVKREEIVTDLDCPKC
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GQGKLVIRFGRNGEFLACSRYNREGDGESCDFTSDFHRDEQGQIVIDKASAPETSDVLCN
CCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCEEEECCCCCCHHHHHHH
VCGRPMVIKKSRFGPFLGCSGYPECNNTRRIGRDGKPVPLPEPTGVQCPKCGEGELLRRR
HCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHC
GKFGRPFYGCSRYPKCDYITNTLDEAQAGEAPAEPAKNEPALPSPSRSSKRTRKSA
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]