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The map label for this gene is dosC [H]

Identifier: 222527438

GI number: 222527438

Start: 5239909

End: 5242074

Strand: Direct

Name: dosC [H]

Synonym: Chy400_4229

Alternate gene names: 222527438

Gene position: 5239909-5242074 (Clockwise)

Preceding gene: 222527437

Following gene: 222527442

Centisome position: 99.45

GC content: 54.76

Gene sequence:

>2166_bases
ATGACACCAGCAACCCCTCCATCCGATACCCCTGAGTCGCGCTGGCCGGAATTAAGCACTGTAGCGGCAATTGACATACT
TGATCAATTGCCTTTCGCAGTATGGATTGTCGATACCGGCTACCGTCTCGTCTACGCTAATCCGGCTTTCATTACTCAAT
CCCCACCAGACATACCTCGTCCAATTCGTACCGGTACACGAATGCTACCGGACGAAAGTGATGTCACTCAGCACCAGCGC
TGGCAGCAGAGCTACCGGCGTGCGCTAGATGGCGAGCATGTCACAATAACCACCAACACGACCACCTATCACCTGCAATT
GATCACGATAGACCCGGTACGGCAGTGGATTCTCGGCTACGCCGGCACCTTTCCAACAACGGAACATGACACGCGCTTCC
GGGCTGCGATTGAAACACTGCCTGATCCATTTGGCATGTATGAAGTCATTCGCAACCCGCGGAACCAGATTGTTGATGCA
CAAATCCTGGTTGTCAATGCGGCAATGTGTCAGATTACCGGGTATCGACGCGAAGAACTGCTCGGCCAGCGCATGCTCGA
TCTCTGGCCCGAATTTACCAACGACGGCACCTTTGCCAGTCTGCGTCACCTGGTTGAGTCAGGAGCACCGGTACGACGTA
CCACGACCCTCCTTGATCGTCATGGTGTGCAGCGGCATATCGAATTGCATGCCGGCAAGCTCGCCAATGGCTTTGTATCG
ATCTGGCGTGATATAACTGCTCAGCAGCAGCTCGATCAAAAGATACGACAGCAAGCCGAACTCCTTGATCAACTTGTTGA
TGCCGTCATTGCGACCGATCTCGATTATGTCGTTACCAGTTGGAACCGTGGAGCCGAACGCATATACGGCTGGTCGGCCA
GCGAAATGATTGGTCGGCGCTTAGGTGAACGTATTGAAACACGCTTCGCCGACACCAATTCCGAAGAGGCGACTGCAATT
TTGTGGCAGAGCGGACGCTGGGAAGGTGAAGTGGAGCAACGGCGACGCGATGGGCAGTATGTGCCGATCCATTCAATTGT
CGTACTGATTCGTGATAAACAAGGCACACCCACCGGCATCGTGGCCGTCAACCGTGACATCAGCGAGCGTCGTCATTTCG
AGCAGTTACTGATGAAAGCCAATCAGCGCCTCGAACAAGCCATCGTCGAAGCCCGCCGCCAGACCGCCGAAATCATGATG
GTTAACGAATTACACGACCTCTTGCAGGTTTGTCAGACCGCAACGGAAGCGGCGGAGGTCATCTCCTTCAGCCTGCAACG
CATCTTTCCACGCCAGAGTGGGTATCTGATGGTACGTCAGGCCGGAACGGCCAATCTCAATTTACTGGCCCAATGGGGCG
AAGCAGACCTTGCACCGGCAACCATGACCATCGATGAATGTTGGGCGCTACGCCGGGGATTAATCCATCGCACCTGTACC
AATGCAGGCATTCGCTGCCCACATATTCACAGCAACACCCCGGCCTGCACCTCCTGTATCCCTCTTGTTGTGCAGAGTGA
AATTTACGGTCTGTTGCACATTGCCGGCGAGGAACTACCACGAAGCGAACTGATTGTTATGACCGGCGATACGATTAAAC
TGGCGTTGTCCAACCTCGAACTGAGAGCCACCCTGCGTGAGCAGGCGATCATCGATCCGCTGACCGGCCTCTACAATCGC
CGCTATCTGGAGAGCAGTCTGCCACGGGAACTCCACCGGGCGCAGCGCGAGCAGTTACCGCTGACGATAGCGATGATTGA
TATTGATCATTTTAAGCAATTCAACGATACGTATGGCCACGACGCCGGCGACGTTCTCTTGCGCGAACTGGCCGTAATTT
TTCGCGAGCATCTGCGTCAAAGCGACCTGGCCTGCCGTTACGGTGGCGAGGAATTTGTCCTGATACTACCGGGAGTCAAT
CGGGAAACTGCCCGTACTCGCTTACAGCAACTCGCCAATACGGTACAGCAGCGCCGAATCACGTTCGCCAACCAACTGAT
AGGACCGGTGACCATATCGATTGGCTACATTACGTATGAGAGAGGTGAGTATCACATCACCACCCTCATCCAAAGCGCCG
ACGCCGCGCTTTATGCAGCCAAGCGCGGCGGACGCAATCGCGTGATTGATTTTGCCGATCTACCGCCCGACCAGGGGGAT
GAATGA

Upstream 100 bases:

>100_bases
TTATTCATTGCCTCTGAGTATCAATAAATCTTCTCGTTCAATATGATAGGAATGAGTTATGATATAAAAAAGCATCGATC
TCGCGTCGCAGGAGAGCGCG

Downstream 100 bases:

>100_bases
CTTGATGGGGGACTGCGTCACGGTGACTCCCACCCCATTACTGAAATCACTACATACCAGCTCATTGCAGGCACGCAGGC
GTAACGTATACTGCCCCTGA

Product: PAS/PAC sensor diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MTPATPPSDTPESRWPELSTVAAIDILDQLPFAVWIVDTGYRLVYANPAFITQSPPDIPRPIRTGTRMLPDESDVTQHQR
WQQSYRRALDGEHVTITTNTTTYHLQLITIDPVRQWILGYAGTFPTTEHDTRFRAAIETLPDPFGMYEVIRNPRNQIVDA
QILVVNAAMCQITGYRREELLGQRMLDLWPEFTNDGTFASLRHLVESGAPVRRTTTLLDRHGVQRHIELHAGKLANGFVS
IWRDITAQQQLDQKIRQQAELLDQLVDAVIATDLDYVVTSWNRGAERIYGWSASEMIGRRLGERIETRFADTNSEEATAI
LWQSGRWEGEVEQRRRDGQYVPIHSIVVLIRDKQGTPTGIVAVNRDISERRHFEQLLMKANQRLEQAIVEARRQTAEIMM
VNELHDLLQVCQTATEAAEVISFSLQRIFPRQSGYLMVRQAGTANLNLLAQWGEADLAPATMTIDECWALRRGLIHRTCT
NAGIRCPHIHSNTPACTSCIPLVVQSEIYGLLHIAGEELPRSELIVMTGDTIKLALSNLELRATLREQAIIDPLTGLYNR
RYLESSLPRELHRAQREQLPLTIAMIDIDHFKQFNDTYGHDAGDVLLRELAVIFREHLRQSDLACRYGGEEFVLILPGVN
RETARTRLQQLANTVQQRRITFANQLIGPVTISIGYITYERGEYHITTLIQSADAALYAAKRGGRNRVIDFADLPPDQGD
E

Sequences:

>Translated_721_residues
MTPATPPSDTPESRWPELSTVAAIDILDQLPFAVWIVDTGYRLVYANPAFITQSPPDIPRPIRTGTRMLPDESDVTQHQR
WQQSYRRALDGEHVTITTNTTTYHLQLITIDPVRQWILGYAGTFPTTEHDTRFRAAIETLPDPFGMYEVIRNPRNQIVDA
QILVVNAAMCQITGYRREELLGQRMLDLWPEFTNDGTFASLRHLVESGAPVRRTTTLLDRHGVQRHIELHAGKLANGFVS
IWRDITAQQQLDQKIRQQAELLDQLVDAVIATDLDYVVTSWNRGAERIYGWSASEMIGRRLGERIETRFADTNSEEATAI
LWQSGRWEGEVEQRRRDGQYVPIHSIVVLIRDKQGTPTGIVAVNRDISERRHFEQLLMKANQRLEQAIVEARRQTAEIMM
VNELHDLLQVCQTATEAAEVISFSLQRIFPRQSGYLMVRQAGTANLNLLAQWGEADLAPATMTIDECWALRRGLIHRTCT
NAGIRCPHIHSNTPACTSCIPLVVQSEIYGLLHIAGEELPRSELIVMTGDTIKLALSNLELRATLREQAIIDPLTGLYNR
RYLESSLPRELHRAQREQLPLTIAMIDIDHFKQFNDTYGHDAGDVLLRELAVIFREHLRQSDLACRYGGEEFVLILPGVN
RETARTRLQQLANTVQQRRITFANQLIGPVTISIGYITYERGEYHITTLIQSADAALYAAKRGGRNRVIDFADLPPDQGD
E
>Mature_720_residues
TPATPPSDTPESRWPELSTVAAIDILDQLPFAVWIVDTGYRLVYANPAFITQSPPDIPRPIRTGTRMLPDESDVTQHQRW
QQSYRRALDGEHVTITTNTTTYHLQLITIDPVRQWILGYAGTFPTTEHDTRFRAAIETLPDPFGMYEVIRNPRNQIVDAQ
ILVVNAAMCQITGYRREELLGQRMLDLWPEFTNDGTFASLRHLVESGAPVRRTTTLLDRHGVQRHIELHAGKLANGFVSI
WRDITAQQQLDQKIRQQAELLDQLVDAVIATDLDYVVTSWNRGAERIYGWSASEMIGRRLGERIETRFADTNSEEATAIL
WQSGRWEGEVEQRRRDGQYVPIHSIVVLIRDKQGTPTGIVAVNRDISERRHFEQLLMKANQRLEQAIVEARRQTAEIMMV
NELHDLLQVCQTATEAAEVISFSLQRIFPRQSGYLMVRQAGTANLNLLAQWGEADLAPATMTIDECWALRRGLIHRTCTN
AGIRCPHIHSNTPACTSCIPLVVQSEIYGLLHIAGEELPRSELIVMTGDTIKLALSNLELRATLREQAIIDPLTGLYNRR
YLESSLPRELHRAQREQLPLTIAMIDIDHFKQFNDTYGHDAGDVLLRELAVIFREHLRQSDLACRYGGEEFVLILPGVNR
ETARTRLQQLANTVQQRRITFANQLIGPVTISIGYITYERGEYHITTLIQSADAALYAAKRGGRNRVIDFADLPPDQGDE

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=40.8536585365854, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786584, Length=156, Percent_Identity=38.4615384615385, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI87082007, Length=188, Percent_Identity=34.5744680851064, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=36.0248447204969, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=36.6279069767442, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=38.3647798742138, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=38.150289017341, Blast_Score=93, Evalue=8e-20,
Organism=Escherichia coli, GI1787802, Length=179, Percent_Identity=32.9608938547486, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787541, Length=169, Percent_Identity=31.3609467455621, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=31.5151515151515, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081974, Length=177, Percent_Identity=29.9435028248588, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=173, Percent_Identity=29.4797687861272, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788956, Length=163, Percent_Identity=32.5153374233129, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 81850; Mature: 81719

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPATPPSDTPESRWPELSTVAAIDILDQLPFAVWIVDTGYRLVYANPAFITQSPPDIPR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEEECCEEECCCCCCCCC
PIRTGTRMLPDESDVTQHQRWQQSYRRALDGEHVTITTNTTTYHLQLITIDPVRQWILGY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEEECHHHHHHHHH
AGTFPTTEHDTRFRAAIETLPDPFGMYEVIRNPRNQIVDAQILVVNAAMCQITGYRREEL
CCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCEEEEEEHHHHHHCCCHHHHH
LGQRMLDLWPEFTNDGTFASLRHLVESGAPVRRTTTLLDRHGVQRHIELHAGKLANGFVS
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHCCHHHHHHHH
IWRDITAQQQLDQKIRQQAELLDQLVDAVIATDLDYVVTSWNRGAERIYGWSASEMIGRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHCCCCHHHHHHHH
LGERIETRFADTNSEEATAILWQSGRWEGEVEQRRRDGQYVPIHSIVVLIRDKQGTPTGI
HHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHCCCCEEEHHHEEEEEECCCCCCCEE
VAVNRDISERRHFEQLLMKANQRLEQAIVEARRQTAEIMMVNELHDLLQVCQTATEAAEV
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISFSLQRIFPRQSGYLMVRQAGTANLNLLAQWGEADLAPATMTIDECWALRRGLIHRTCT
HHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHC
NAGIRCPHIHSNTPACTSCIPLVVQSEIYGLLHIAGEELPRSELIVMTGDTIKLALSNLE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEHHHCCH
LRATLREQAIIDPLTGLYNRRYLESSLPRELHRAQREQLPLTIAMIDIDHFKQFNDTYGH
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEEHHHHHHHCCCCCC
DAGDVLLRELAVIFREHLRQSDLACRYGGEEFVLILPGVNRETARTRLQQLANTVQQRRI
CHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
TFANQLIGPVTISIGYITYERGEYHITTLIQSADAALYAAKRGGRNRVIDFADLPPDQGD
HHHHHHCCCEEEEEEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
E
C
>Mature Secondary Structure 
TPATPPSDTPESRWPELSTVAAIDILDQLPFAVWIVDTGYRLVYANPAFITQSPPDIPR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCEEEEECCEEECCCCCCCCC
PIRTGTRMLPDESDVTQHQRWQQSYRRALDGEHVTITTNTTTYHLQLITIDPVRQWILGY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEEECHHHHHHHHH
AGTFPTTEHDTRFRAAIETLPDPFGMYEVIRNPRNQIVDAQILVVNAAMCQITGYRREEL
CCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCHHHHCCCEEEEEEHHHHHHCCCHHHHH
LGQRMLDLWPEFTNDGTFASLRHLVESGAPVRRTTTLLDRHGVQRHIELHAGKLANGFVS
HHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHCCHHHHHHHH
IWRDITAQQQLDQKIRQQAELLDQLVDAVIATDLDYVVTSWNRGAERIYGWSASEMIGRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHCCCCHHHHHHHH
LGERIETRFADTNSEEATAILWQSGRWEGEVEQRRRDGQYVPIHSIVVLIRDKQGTPTGI
HHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHCCCCEEEHHHEEEEEECCCCCCCEE
VAVNRDISERRHFEQLLMKANQRLEQAIVEARRQTAEIMMVNELHDLLQVCQTATEAAEV
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISFSLQRIFPRQSGYLMVRQAGTANLNLLAQWGEADLAPATMTIDECWALRRGLIHRTCT
HHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHC
NAGIRCPHIHSNTPACTSCIPLVVQSEIYGLLHIAGEELPRSELIVMTGDTIKLALSNLE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEHHHCCH
LRATLREQAIIDPLTGLYNRRYLESSLPRELHRAQREQLPLTIAMIDIDHFKQFNDTYGH
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCEEEEEEEHHHHHHHCCCCCC
DAGDVLLRELAVIFREHLRQSDLACRYGGEEFVLILPGVNRETARTRLQQLANTVQQRRI
CHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
TFANQLIGPVTISIGYITYERGEYHITTLIQSADAALYAAKRGGRNRVIDFADLPPDQGD
HHHHHHCCCEEEEEEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]