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The map label for this gene is yrkH [H]

Identifier: 222527422

GI number: 222527422

Start: 5220515

End: 5221915

Strand: Reverse

Name: yrkH [H]

Synonym: Chy400_4213

Alternate gene names: 222527422

Gene position: 5221915-5220515 (Counterclockwise)

Preceding gene: 222527424

Following gene: 222527421

Centisome position: 99.11

GC content: 56.32

Gene sequence:

>1401_bases
ATGTTGCTGAAGTATTTCTACGACGAGATGCTGGCGCAGGCTTCGTACCTCATCGGCTGCGCTAAGACCGGTGAGGCAAT
GGTCATCGATCCAATGCGCGATGTAGAGCCGTATTTGCGCGTAGCCGCCAAAGAAGGATTGCGGATTACCCATGTAACGG
AAACTCATATTCACGCCGATTTTGTCAGCGGTGTCCGTGAGCTGGCTGCCCGTACCGGTGCCCAGATGTATCTGAGCGAT
ATGGGCGATGCCAACTGGAAGTATGCCTACCCAGAAATTGAGAAGGCCATTCTGGTGAAGGATGGCGATACGTGGATGGT
TGGCAACATTAAGGTACAGGTGATTGCAACCCCCGGCCATACCCCAGAGCACATTGCTTTCATGATTACCGATACTGCCG
GTGCCGATCAGCCGATGGGTGTGTTCACCGGTGATTTTCTGTTCGTCGGTGATGTTGGGCGCCCTGATTTGCTGGAAGAG
GCCGCCGGTATTTCTGGTACGAAGGAGCCGGGTGCACGCAAACAGTTCCAGTCGGTGCGACGGTTTGCCGAACTTCCTGA
CTATTTGCAGGTGTGGCCGGCTCACGGTGCAGGCAGCGCGTGTGGCAAGGCGTTGGGCGCCATTCCATCGAGCACACTCG
GTTACGAGAAGCGTTTCAACCCGGCGTTCCAGTTTGACGATGAAGATGCGTTTGTGCGCTGGTTGCTGAAGGGTCAACCG
GAACCACCGCGTTACTTTGCCCGCATGAAGTATGTCAACAAAGTCGGGCCGGCGCTTTTGCACGATCTGGCAACCCCGGT
AGAGGTCGAGCGGCCACTTCTCGATCAGGCAATGGCAGAAGGGGCAATGGTCTTTGATCTGCGCAGTGCTGCCGAGTTTG
TCGCCGGTCACGTTCCGGGCAGCATCAGTGTTCCCATTCGCAAGATGTACAGCACATATGTAGGCTGGTTCGTCGATTTC
AACAAGCCCACCTACCTGGTTGTGCCGGATGGCGCCGACATGAATCAGATTTTGAAGGATTTGCGGGCGATTGGGGTGGA
TGATATTCCCGGTTACCTGCCGGCGTCAGCGCTCGGTGATAATCTCGTGCAGTTGCCGACCGCTACGGTGAAAGACCTGA
GCGCCGCTCTGAGCGATCATTCGGCAGTCATTCTCGATATGCGTAACCTGACCGAATACGAAGAGATTCATCTGCCCGGT
GCGCTGCACATTCCGCTCGGCTATCTGCCGCGCCGCCTGGACGACATTCCACGGGATACCCCCATTATTGCGCACTGTGC
AACCGGCTATCGCTCACAGGTTGGCACGAGCTTACTGCGCCGGTTTGGTTTTACCAACGTAGCGACGCTGGTCGATCCGC
AGGGCACCTGGCGAGAACTGGCTTCAGCAGTTGCTGTTTGA

Upstream 100 bases:

>100_bases
ACTGTCAAATCCCAACACTCTCAACAAGCGATTGGCGCCAAAGCTGTCATACGGGATCGGGTACGGCTAATCATACGTGA
AAAAGAAGGGAAGCAAAGCG

Downstream 100 bases:

>100_bases
CAGCGTGCTGTTTCTGCCGGTAGGAGTGGCCATTACTACCGGCAGAAACAACAGCGAGGCGAATAGTGGTTAGCCCGCTG
CGTAGCGGGCGCAAGACGCA

Product: beta-lactamase domain-containing protein

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MLLKYFYDEMLAQASYLIGCAKTGEAMVIDPMRDVEPYLRVAAKEGLRITHVTETHIHADFVSGVRELAARTGAQMYLSD
MGDANWKYAYPEIEKAILVKDGDTWMVGNIKVQVIATPGHTPEHIAFMITDTAGADQPMGVFTGDFLFVGDVGRPDLLEE
AAGISGTKEPGARKQFQSVRRFAELPDYLQVWPAHGAGSACGKALGAIPSSTLGYEKRFNPAFQFDDEDAFVRWLLKGQP
EPPRYFARMKYVNKVGPALLHDLATPVEVERPLLDQAMAEGAMVFDLRSAAEFVAGHVPGSISVPIRKMYSTYVGWFVDF
NKPTYLVVPDGADMNQILKDLRAIGVDDIPGYLPASALGDNLVQLPTATVKDLSAALSDHSAVILDMRNLTEYEEIHLPG
ALHIPLGYLPRRLDDIPRDTPIIAHCATGYRSQVGTSLLRRFGFTNVATLVDPQGTWRELASAVAV

Sequences:

>Translated_466_residues
MLLKYFYDEMLAQASYLIGCAKTGEAMVIDPMRDVEPYLRVAAKEGLRITHVTETHIHADFVSGVRELAARTGAQMYLSD
MGDANWKYAYPEIEKAILVKDGDTWMVGNIKVQVIATPGHTPEHIAFMITDTAGADQPMGVFTGDFLFVGDVGRPDLLEE
AAGISGTKEPGARKQFQSVRRFAELPDYLQVWPAHGAGSACGKALGAIPSSTLGYEKRFNPAFQFDDEDAFVRWLLKGQP
EPPRYFARMKYVNKVGPALLHDLATPVEVERPLLDQAMAEGAMVFDLRSAAEFVAGHVPGSISVPIRKMYSTYVGWFVDF
NKPTYLVVPDGADMNQILKDLRAIGVDDIPGYLPASALGDNLVQLPTATVKDLSAALSDHSAVILDMRNLTEYEEIHLPG
ALHIPLGYLPRRLDDIPRDTPIIAHCATGYRSQVGTSLLRRFGFTNVATLVDPQGTWRELASAVAV
>Mature_466_residues
MLLKYFYDEMLAQASYLIGCAKTGEAMVIDPMRDVEPYLRVAAKEGLRITHVTETHIHADFVSGVRELAARTGAQMYLSD
MGDANWKYAYPEIEKAILVKDGDTWMVGNIKVQVIATPGHTPEHIAFMITDTAGADQPMGVFTGDFLFVGDVGRPDLLEE
AAGISGTKEPGARKQFQSVRRFAELPDYLQVWPAHGAGSACGKALGAIPSSTLGYEKRFNPAFQFDDEDAFVRWLLKGQP
EPPRYFARMKYVNKVGPALLHDLATPVEVERPLLDQAMAEGAMVFDLRSAAEFVAGHVPGSISVPIRKMYSTYVGWFVDF
NKPTYLVVPDGADMNQILKDLRAIGVDDIPGYLPASALGDNLVQLPTATVKDLSAALSDHSAVILDMRNLTEYEEIHLPG
ALHIPLGYLPRRLDDIPRDTPIIAHCATGYRSQVGTSLLRRFGFTNVATLVDPQGTWRELASAVAV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=238, Percent_Identity=26.890756302521, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI94538322, Length=142, Percent_Identity=31.6901408450704, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI94538320, Length=142, Percent_Identity=31.6901408450704, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1787158, Length=228, Percent_Identity=28.9473684210526, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17538952, Length=252, Percent_Identity=26.5873015873016, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI221330176, Length=237, Percent_Identity=26.1603375527426, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667703, Length=231, Percent_Identity=32.034632034632, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI21356335, Length=231, Percent_Identity=32.034632034632, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24667711, Length=231, Percent_Identity=32.034632034632, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 51143; Mature: 51143

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKYFYDEMLAQASYLIGCAKTGEAMVIDPMRDVEPYLRVAAKEGLRITHVTETHIHAD
CHHHHHHHHHHHHHHHHEEECCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
FVSGVRELAARTGAQMYLSDMGDANWKYAYPEIEKAILVKDGDTWMVGNIKVQVIATPGH
HHHHHHHHHHHCCHHHHHHHCCCCCCEEECCCCCEEEEEECCCEEEEEEEEEEEEECCCC
TPEHIAFMITDTAGADQPMGVFTGDFLFVGDVGRPDLLEEAAGISGTKEPGARKQFQSVR
CCCEEEEEEEECCCCCCCCCEEECCEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHH
RFAELPDYLQVWPAHGAGSACGKALGAIPSSTLGYEKRFNPAFQFDDEDAFVRWLLKGQP
HHHHCCHHHEECCCCCCCHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHCCCC
EPPRYFARMKYVNKVGPALLHDLATPVEVERPLLDQAMAEGAMVFDLRSAAEFVAGHVPG
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCC
SISVPIRKMYSTYVGWFVDFNKPTYLVVPDGADMNQILKDLRAIGVDDIPGYLPASALGD
CCCHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHCC
NLVQLPTATVKDLSAALSDHSAVILDMRNLTEYEEIHLPGALHIPLGYLPRRLDDIPRDT
CCEECCCHHHHHHHHHHCCCCEEEEEHHCCCCHHHCCCCCEEECCHHHHHHHHHCCCCCC
PIIAHCATGYRSQVGTSLLRRFGFTNVATLVDPQGTWRELASAVAV
CEEEEHHCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCC
>Mature Secondary Structure
MLLKYFYDEMLAQASYLIGCAKTGEAMVIDPMRDVEPYLRVAAKEGLRITHVTETHIHAD
CHHHHHHHHHHHHHHHHEEECCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
FVSGVRELAARTGAQMYLSDMGDANWKYAYPEIEKAILVKDGDTWMVGNIKVQVIATPGH
HHHHHHHHHHHCCHHHHHHHCCCCCCEEECCCCCEEEEEECCCEEEEEEEEEEEEECCCC
TPEHIAFMITDTAGADQPMGVFTGDFLFVGDVGRPDLLEEAAGISGTKEPGARKQFQSVR
CCCEEEEEEEECCCCCCCCCEEECCEEEEECCCCCHHHHHHCCCCCCCCCCHHHHHHHHH
RFAELPDYLQVWPAHGAGSACGKALGAIPSSTLGYEKRFNPAFQFDDEDAFVRWLLKGQP
HHHHCCHHHEECCCCCCCHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHCCCC
EPPRYFARMKYVNKVGPALLHDLATPVEVERPLLDQAMAEGAMVFDLRSAAEFVAGHVPG
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCC
SISVPIRKMYSTYVGWFVDFNKPTYLVVPDGADMNQILKDLRAIGVDDIPGYLPASALGD
CCCHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHCC
NLVQLPTATVKDLSAALSDHSAVILDMRNLTEYEEIHLPGALHIPLGYLPRRLDDIPRDT
CCEECCCHHHHHHHHHHCCCCEEEEEHHCCCCHHHCCCCCEEECCHHHHHHHHHCCCCCC
PIIAHCATGYRSQVGTSLLRRFGFTNVATLVDPQGTWRELASAVAV
CEEEEHHCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]