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The map label for this gene is aceA [H]

Identifier: 222527412

GI number: 222527412

Start: 5212031

End: 5213305

Strand: Reverse

Name: aceA [H]

Synonym: Chy400_4203

Alternate gene names: 222527412

Gene position: 5213305-5212031 (Counterclockwise)

Preceding gene: 222527413

Following gene: 222527411

Centisome position: 98.94

GC content: 58.75

Gene sequence:

>1275_bases
ATGGATCGTGCCGCACAGATCAAGCAGATTGCCGACAGTTGGAACACGCCGCGTTTTGCCGGCATTGTGCGCCCCTACAC
GCCGGAGGATGTGTATCGTCTGCGGGGTTCGGTGCAGATTGAGTACACCCTGGCGCGGATGGGTGCCGAGCGCTTGTGGA
ACCTCTTGCACACCGAGCCGTACATCAATGCCCTGGGTGCCCTAACGGGTAATCAGGCAATGCAGCAGGTGAAAGCCGGT
CTGAAAGCCATCTACCTGAGTGGCTGGCAGGTAGCCGCCGATGCCAATCTGGCGGGGCAGATGTATCCCGATCAGAGCCT
CTATCCGGCAAATTCAGGCCCCCAACTGGTGCGCAACATCAACAATGCCCTCCGCCGTGCCGACCAGATTTATCACAGCG
AAGGGCGAAATGACATTTACTGGTTTGCCCCGATTGTGGCCGATGCCGAAGCCGGCTTTGGTGGCCCACTCAATGTCTTC
GAGATTATGAAGGCGTATATCGAAGCCGGTGCGGCAGGTGTACACTTCGAGGATCAGCTTGCCTCCGAAAAGAAGTGTGG
TCATATGGGCGGGAAGGTGTTGATCCCCACCCAGGCTGCGATTCGTAATCTGGTGGCGGCTCGTCTGGCAGCCGATGTGA
TGGGGGTGCCGACGATCATTGTGGCGCGCACCGACGCAAATGCGGCTACGTTGCTCACCAGTGATATTGATGAGCGGGAT
CGGCCCTTCTGCACCGGCGAGCGCACCAGCGAGGGCTTCTACCGGGTGCGGGCGGGCCTTGATCAGGCGATTGCCCGTGG
TCTGGCCTATGCCCCCTACGCCGATATGATCTGGTGTGAAACGAGCGAGCCAAATCTCGAAGAGGCACGCCGCTTTGCCG
AGGCTATTCACGCCCAATTCCCTGGCAAGTTGCTGGCCTACAACTGTTCGCCATCGTTCAACTGGAAGAAGAAGCTGGAC
GATGCGACGATTGCGGCCTTCCAGCGCGAATTGGGTGCCATGGGCTACAAGTTCCAATTCGTGACCCTGGCCGGCTTCCA
CGCGCTGAACTACAGCATGTTTGAACTGGCGCGCAACTATCGTGATCGGGGGATGGCTGCCTACAGCGAATTGCAACAGG
CGGAATTTGCCGCAGAAGCCTACGGTTACACGGCGACCCGTCACCAGCGTGAAGTTGGGACCGGTTATTTCGATGAGGTG
GCGCAGGTGATTGCCGGTGGCGAGATCAGCACAACGGCATTGACCGGCAGTACTGAGGAAGAGCAGTTCCACTAG

Upstream 100 bases:

>100_bases
AAGATGTAACTACCCCGTAACGCAGAGTGTTATACTGCTAAGCATCAATACTTTTGAGACCGTGTTCGTCAGATTCAGCG
CAGAAATTGGAGAAACGCAC

Downstream 100 bases:

>100_bases
CGGTAGCGGATTAATACCCGATCAACAGCTTCTGACGCGAATATCCGTCCAGGGGGCAACCATCTGATTGCCCCCTGGAT
GTTTTGTCCGTTGTGTGCAG

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MDRAAQIKQIADSWNTPRFAGIVRPYTPEDVYRLRGSVQIEYTLARMGAERLWNLLHTEPYINALGALTGNQAMQQVKAG
LKAIYLSGWQVAADANLAGQMYPDQSLYPANSGPQLVRNINNALRRADQIYHSEGRNDIYWFAPIVADAEAGFGGPLNVF
EIMKAYIEAGAAGVHFEDQLASEKKCGHMGGKVLIPTQAAIRNLVAARLAADVMGVPTIIVARTDANAATLLTSDIDERD
RPFCTGERTSEGFYRVRAGLDQAIARGLAYAPYADMIWCETSEPNLEEARRFAEAIHAQFPGKLLAYNCSPSFNWKKKLD
DATIAAFQRELGAMGYKFQFVTLAGFHALNYSMFELARNYRDRGMAAYSELQQAEFAAEAYGYTATRHQREVGTGYFDEV
AQVIAGGEISTTALTGSTEEEQFH

Sequences:

>Translated_424_residues
MDRAAQIKQIADSWNTPRFAGIVRPYTPEDVYRLRGSVQIEYTLARMGAERLWNLLHTEPYINALGALTGNQAMQQVKAG
LKAIYLSGWQVAADANLAGQMYPDQSLYPANSGPQLVRNINNALRRADQIYHSEGRNDIYWFAPIVADAEAGFGGPLNVF
EIMKAYIEAGAAGVHFEDQLASEKKCGHMGGKVLIPTQAAIRNLVAARLAADVMGVPTIIVARTDANAATLLTSDIDERD
RPFCTGERTSEGFYRVRAGLDQAIARGLAYAPYADMIWCETSEPNLEEARRFAEAIHAQFPGKLLAYNCSPSFNWKKKLD
DATIAAFQRELGAMGYKFQFVTLAGFHALNYSMFELARNYRDRGMAAYSELQQAEFAAEAYGYTATRHQREVGTGYFDEV
AQVIAGGEISTTALTGSTEEEQFH
>Mature_424_residues
MDRAAQIKQIADSWNTPRFAGIVRPYTPEDVYRLRGSVQIEYTLARMGAERLWNLLHTEPYINALGALTGNQAMQQVKAG
LKAIYLSGWQVAADANLAGQMYPDQSLYPANSGPQLVRNINNALRRADQIYHSEGRNDIYWFAPIVADAEAGFGGPLNVF
EIMKAYIEAGAAGVHFEDQLASEKKCGHMGGKVLIPTQAAIRNLVAARLAADVMGVPTIIVARTDANAATLLTSDIDERD
RPFCTGERTSEGFYRVRAGLDQAIARGLAYAPYADMIWCETSEPNLEEARRFAEAIHAQFPGKLLAYNCSPSFNWKKKLD
DATIAAFQRELGAMGYKFQFVTLAGFHALNYSMFELARNYRDRGMAAYSELQQAEFAAEAYGYTATRHQREVGTGYFDEV
AQVIAGGEISTTALTGSTEEEQFH

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=431, Percent_Identity=67.0533642691415, Blast_Score=587, Evalue=1e-169,
Organism=Escherichia coli, GI1786525, Length=262, Percent_Identity=28.2442748091603, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17561814, Length=427, Percent_Identity=66.0421545667447, Blast_Score=580, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI71982926, Length=427, Percent_Identity=66.0421545667447, Blast_Score=580, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6320908, Length=297, Percent_Identity=37.037037037037, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325263, Length=257, Percent_Identity=34.2412451361868, Blast_Score=152, Evalue=8e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 46833; Mature: 46833

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRAAQIKQIADSWNTPRFAGIVRPYTPEDVYRLRGSVQIEYTLARMGAERLWNLLHTEP
CCHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHCCCEEEEEHHHHHCHHHHHHHHHCCH
YINALGALTGNQAMQQVKAGLKAIYLSGWQVAADANLAGQMYPDQSLYPANSGPQLVRNI
HHHHHHHHCCHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
NNALRRADQIYHSEGRNDIYWFAPIVADAEAGFGGPLNVFEIMKAYIEAGAAGVHFEDQL
HHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
ASEKKCGHMGGKVLIPTQAAIRNLVAARLAADVMGVPTIIVARTDANAATLLTSDIDERD
HHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCC
RPFCTGERTSEGFYRVRAGLDQAIARGLAYAPYADMIWCETSEPNLEEARRFAEAIHAQF
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHC
PGKLLAYNCSPSFNWKKKLDDATIAAFQRELGAMGYKFQFVTLAGFHALNYSMFELARNY
CCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHH
RDRGMAAYSELQQAEFAAEAYGYTATRHQREVGTGYFDEVAQVIAGGEISTTALTGSTEE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCC
EQFH
CCCC
>Mature Secondary Structure
MDRAAQIKQIADSWNTPRFAGIVRPYTPEDVYRLRGSVQIEYTLARMGAERLWNLLHTEP
CCHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHCCCEEEEEHHHHHCHHHHHHHHHCCH
YINALGALTGNQAMQQVKAGLKAIYLSGWQVAADANLAGQMYPDQSLYPANSGPQLVRNI
HHHHHHHHCCHHHHHHHHHHHHEEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
NNALRRADQIYHSEGRNDIYWFAPIVADAEAGFGGPLNVFEIMKAYIEAGAAGVHFEDQL
HHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
ASEKKCGHMGGKVLIPTQAAIRNLVAARLAADVMGVPTIIVARTDANAATLLTSDIDERD
HHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCC
RPFCTGERTSEGFYRVRAGLDQAIARGLAYAPYADMIWCETSEPNLEEARRFAEAIHAQF
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHC
PGKLLAYNCSPSFNWKKKLDDATIAAFQRELGAMGYKFQFVTLAGFHALNYSMFELARNY
CCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHH
RDRGMAAYSELQQAEFAAEAYGYTATRHQREVGTGYFDEVAQVIAGGEISTTALTGSTEE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCC
EQFH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]